What Can an Organ-on-a-Chip Teach Us About Human Lung Pathophysiology? DOI
Haiqing Bai, Donald E. Ingber

Physiology, Journal Year: 2022, Volume and Issue: 37(5), P. 242 - 252

Published: June 6, 2022

The intertwined relationship between structure and function has been key to understanding human organ physiology disease pathogenesis. An organ-on-a-chip (organ chip) is a bioengineered microfluidic cell culture device lined by living cells tissues that recapitulates organ-level functions in vitro. This accomplished recreating organ-specific tissue-tissue interfaces microenvironmental biochemical mechanical cues while providing dynamic perfusion through endothelium-lined vascular channels. In this review, we discuss how emerging technology contributed the of lung structure-function relationships at cell, tissue, levels.

Language: Английский

Biomedical Applications of Microfluidic Devices: A Review DOI Creative Commons
Ghazaleh Gharib, İsmail Bütün, Zülâl Muganlı

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 1023 - 1023

Published: Nov. 16, 2022

Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.

Language: Английский

Citations

105
Near-infrared light-responsive multifunctional hydrogel releasing peptide-functionalized gold nanorods sequentially for diabetic wound healing DOI
Xiaoqiong Huang, Lei Xu,

Xiaoran Yu

et al.

Journal of Colloid and Interface Science, Journal Year: 2023, Volume and Issue: 639, P. 369 - 384

Published: Feb. 16, 2023

Language: Английский

Citations

50
Human organoids-on-chips for biomedical research and applications DOI Creative Commons
Hui Wang,

Xiufan Ning,

Feng Zhao

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(2), P. 788 - 818

Published: Jan. 1, 2024

Human organoids-on-chips (OrgOCs) are the synergism of human organoids (HOs) technology and microfluidic organs-on-chips (OOCs).OOCs can mimic extrinsic characteristics organs, such as environmental clues living tissue, while HOs more amenable to biological analysis genetic manipulation.By spatial cooperation, OrgOCs served 3D organotypic models allowing them recapitulate critical tissue-specific properties forecast responses outcomes.It represents a giant leap forward from regular 2D cell monolayers animal in improved ecological niche modeling.In recent years, have offered potential promises for clinical studies advanced preclinical-to-clinical translation medical industrial fields.In this review, we highlight cutting-edge achievements OrgOCs, introduce key features architectures, share revolutionary applications basic biology, disease modeling, preclinical assay precision medicine.Furthermore, discuss how combine wide range disciplines with accelerate translational applications, well challenges opportunities biomedical research applications.

Language: Английский

Citations

28
Hydrogel Strain Sensors for Integrating Into Dynamic Organ‐on‐a‐Chip DOI Open Access

Wenqi She,

Chong Shen,

Zhongying Xue

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Current hydrogel strain sensors have never been integrated into dynamic organ‐on‐a‐chip (OOC) due to the lack of sensitivity in aqueous cell culture systems. To enhance sensing performance, a novel sensor is presented which MXene layer coated on bottom surface pre‐stretched anti‐swelling substrate di‐acrylated Pluronic F127 (F127‐DA) and chitosan (CS) for isolation from top surface. The fabricated display high (gauge factor 290.96), wide range (0–100%), repeatability. demonstrate its application, alveolar epithelial cells are cultivated forming barriers, then lung‐on‐a‐chip (LOC) This system can sensitively monitor normal physiological breathing, pathological inflammation stimulated by lipopolysaccharide (LPS), alleviated through drug intervention.

Language: Английский

Citations

3
Pulmonary delivery nanomedicines towards circumventing physiological barriers: Strategies and characterization approaches DOI
Wenhao Wang, Zhengwei Huang, Ying Huang

et al.

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 185, P. 114309 - 114309

Published: April 22, 2022

Language: Английский

Citations

57
3D Inkjet-Bioprinted Lung-on-a-Chip DOI
Wookyeom Kim, Yunji Lee, Dayoon Kang

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(5), P. 2806 - 2815

Published: April 20, 2023

There is an urgent need for physiologically relevant and customizable biochip models of human lung tissue to provide a niche disease modeling drug efficacy. Although various lung-on-a-chips have been developed, the conventional fabrication method has limited in reconstituting very thin multilayered architecture spatial arrangements multiple cell types microfluidic device. To overcome these limitations, we developed alveolar lung-on-a-chip model, effectively integrated with inkjet-printed, micron-thick, three-layered tissue. After bioprinting tissues inside four culture inserts layer-by-layer, are implanted into that supplies flow medium. This modular implantation procedure enables formation facilitate 3D-structured inkjet-bioprinted under perfusion at air–liquid interface. The bioprinted cultured on chip maintained their structure three layers tens micrometers achieved tight junction epithelial layer, critical properties barrier. upregulation genes involved essential functions alveoli was also confirmed our model. Our insert-mountable organ-on-a-chip versatile platform can be applied organ by implanting replacing inserts. It amenable mass production development customized through convergence technology.

Language: Английский

Citations

34
Developing conductive hydrogels for biomedical applications DOI Creative Commons
Yu Wang, Jiahui Guo, Xinyue Cao

et al.

Smart Medicine, Journal Year: 2023, Volume and Issue: 3(1)

Published: Sept. 15, 2023

Conductive hydrogels have attracted copious attention owing to their grateful performances, such as similarity biological tissues, compliance, conductivity and biocompatibility. A diversity of conductive been developed showed versatile potentials in biomedical applications. In this review, we highlight the recent advances hydrogels, involving various types functionalities well applications fields. Furthermore, current challenges reasonable outlook are also given. It is expected that review will provide potential guidance for advancement next-generation hydrogels.

Language: Английский

Citations

27
Recent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and Therapeutics DOI
Ashwini Shinde, Kavitha Illath, Uvanesh Kasiviswanathan

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3121 - 3146

Published: Jan. 30, 2023

ADVERTISEMENT RETURN TO ISSUEReviewNEXTRecent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and TherapeuticsAshwini ShindeAshwini ShindeDepartment Engineering Design, Indian Institute Technology Madras, Chennai 600036, IndiaMore by Ashwini ShindeView Biography, Kavitha IllathKavitha IllathDepartment IllathView Uvanesh KasiviswanathanUvanesh KasiviswanathanDepartment KasiviswanathanView Biographyhttps://orcid.org/0000-0003-3535-2244, Shalini NagabooshanamShalini NagabooshanamDepartment NagabooshanamView Biographyhttps://orcid.org/0000-0003-4856-6815, Pallavi GuptaPallavi GuptaDepartment GuptaView Koyel DeyKoyel DeyDepartment DeyView Pulasta ChakrabartyPulasta ChakrabartyDepartment ChakrabartyView Moeto NagaiMoeto NagaiDepartment Mechanical Engineering, Toyohashi University Technology, 441-8580, JapanMore NagaiView Suresh RaoSuresh RaoDepartment RaoView Srabani Kar*Srabani KarDepartment Physics, Science Education Research (IISER), Tirupati, Andhra Pradesh 517507, India*[email protected]More KarView Tuhin Subhra Santra*Tuhin SantraDepartment protected]; [email SantraView Biographyhttps://orcid.org/0000-0002-9403-2155Cite this: Anal. Chem. 2023, 95, 6, 3121–3146Publication Date (Web):January 30, 2023Publication History Received12 November 2022Published online30 January 2023Published inissue 14 February 2023https://pubs.acs.org/doi/10.1021/acs.analchem.2c05036https://doi.org/10.1021/acs.analchem.2c05036review-articleACS PublicationsCopyright © 2023 American Chemical SocietyRequest reuse permissionsArticle Views3446Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Cells,Electrical properties,Electrodes,Sensors Get e-Alerts

Language: Английский

Citations

25
Organ-on-a-chip for dynamic tumor drug resistance investigation DOI
Changmin Shao, Yunru Yu, Xin Lei

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 460, P. 141739 - 141739

Published: Feb. 6, 2023

Language: Английский

Citations

24
Electroacoustic Responsive Cochlea‐on‐a‐Chip DOI Creative Commons
Yangnan Hu,

Jiayue Xing,

Hui Zhang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(24)

Published: March 16, 2024

Organ-on-chips can highly simulate the complex physiological functions of organs, exhibiting broad application prospects in developmental research, disease simulation, as well new drug research and development. However, there is still less concern about effectively constructing cochlea-on-chips. Here, a novel cochlear organoids-integrated conductive hydrogel biohybrid system with implant electroacoustic stimulation (EAS) for cochlea-on-a-chip construction high-throughput screening, presented. Benefiting from superior biocompatibility electrical property hydrogel, together EAS, inner ear progenitor cells proliferate spontaneously shape into spheres, finally forming organoids good cell viability structurally mature hair cells. By incorporating these cells-encapsulated hydrogels microfluidic-based culture chambers concentration gradient generator, dynamic evaluation disease-related drugs demonstrated. These results indicate that proposed platform has great potential organoid cultivation deafness evaluation.

Language: Английский

Citations

15