Magnesium Gradient‐Based Hierarchical Scaffold for Dual‐Lineage Regeneration of Osteochondral Defect

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(43)

Published: July 2, 2023

Abstract Osteochondral regeneration remains a great challenge due to the limited self‐healing ability and complexity of its hierarchical structure composition. Mg 2+ hypoxia are two effective modulators in boosting chondrogenesis. To this end, double‐layered scaffold (D) consisting hydrogel layer on porous cryogel is fabricated mimic osteochondral tissue. An gradient incorporated into with hypoxia‐mimicking deferoxamine (DFO) embedded (D‐Mg‐DFO), which remarkably augments dual‐lineage both cartilage subchondral bone. The higher supplementation from upper hydrogel, associated situation small pore size, exhibits promotive effects chondrogenic differentiation. lower bottom cryogel, interconnected macroporous structure, achieves multiple contributions stem cell migration bone marrow cavity, matrix mineralization, osteogenesis. Furthermore, rabbits’ trochlea defects established evaluate regenerative outcome. Compared control scaffolds containing only or DFO, D‐Mg‐DFO presents best effect under synergistic contribution factors. Overall, work provides new design toward an repair defect.

Language: Английский

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Full-thickness osteochondral defect repair using a biodegradable bilayered scaffold of porous zinc and chondroitin sulfate hydrogel

Bioactive Materials, Journal Year: 2023, Volume and Issue: 32, P. 400 - 414

Published: Oct. 24, 2023

The regeneration of osteochondral tissue necessitates the re-establishment a gradient owing to unique characteristics and healing potential chondral osseous phases. As self-healing capacity hyaline cartilage is limited, timely mechanical support during neo-cartilage formation crucial achieving optimal repair efficacy. In this study, we devised biodegradable bilayered scaffold, comprising chondroitin sulfate (CS) hydrogel regenerate porous pure zinc (Zn) scaffold for underlying bone as layer. photocured CS possessed compressive strength 82 kPa, while Zn exhibited yield 11 MPa stiffness 0.8 GPa. Such properties are similar values reported cancellous bone. vitro biological experiments demonstrated that displayed favorable cytocompatibility promoted chondrogenic osteogenic differentiation marrow stem cells. Upon implantation, facilitated simultaneous in porcine model, resulting (i) smoother surface, (ii) more hyaline-like cartilage, (iii) superior integration into adjacent host tissue. Our exhibits significant clinical application regeneration.

Language: Английский

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An Encapsulation‐Free and Hierarchical Porous Triboelectric Scaffold with Dynamic Hydrophilicity for Efficient Cartilage Regeneration

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(27)

Published: March 29, 2024

Abstract Tissue engineering and electrotherapy are two promising methods to promote tissue repair. However, their integration remains an underexplored area, because requirements on devices usually distinct. Triboelectric nanogenerators (TENGs) have shown great potential develop self‐powered devices. due susceptibility moisture, TENGs be encapsulated in vivo. Therefore, existing cannot employed as scaffolds, which require direct interaction with surrounding cells. Here, the concept of triboelectric scaffolds (TESs) is proposed. Poly(glycerol sebacate), a biodegradable relatively hydrophobic elastomer, selected matrix TESs. Each micropore multi‐hierarchical porous TESs efficiently serves moisture‐resistant working unit TENGs. Integration tons micropores ensures ability vivo without encapsulation. Originally degraded by surface erosion transformed into hydrophilic surfaces, facilitating role scaffolds. Notably, seeded chondrocytes obtain dense large matured cartilages after subcutaneous implantation nude mice. Importantly, rabbits osteochondral defects receiving TES show favorable hyaline cartilage regeneration complete healing. This work provides electronic biomedical device will inspire series new applications.

Language: Английский

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Mechanics‐Resilient HA/SIS‐Based Composite Scaffolds with ROS‐Scavenging and Bacteria‐Resistant Capacity to Address Infected Bone Regeneration

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(24)

Published: Jan. 23, 2024

Abstract To address and regenerate infected bone defects complicated by issues such as inflammation resorption, to promote regeneration, this study focuses on the development of a composite scaffold with reactive oxygen species (ROS)‐scavenging bacteria‐resistant properties. The integrates self‐assembled small intestinal submucosa (SIS) hydrogel pre‐adsorbed hydroxyapatite (HA) particles tannic acid (TA), demonstrating distinctive mechanical resilience porous structures, suitable for filling irregular cavities facilitating cell infiltration, while exhibiting broad‐spectrum antibacterial efficacy robust ROS‐scavenging capacity tissue regeneration. RNA‐sequencing analysis indicates underlying mechanism revealing disrupting arginine alanine amino biosynthesis. Furthermore, demonstrates excellent cytocompatibility, viability exceeding 70%. Remarkably, it exceptional anti‐inflammatory performances (≈5‐fold control). In an defect model, facilitates superior being ≈5‐fold greater than control, maintaining conducive environment adhesion infiltration without collapse. This multifunctional emerges promising candidate combating infections in showcasing its potential addressing complex bone‐related challenges.

Language: Английский

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Biodegradable Piezoelectric‐Conductive Integrated Hydrogel Scaffold for Repair of Osteochondral Defects

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(45)

Published: Sept. 13, 2024

Abstract Osteochondral injury is a prevalent condition for which no specific treatment currently available. This study presents piezoelectric‐conductive scaffold composed of piezoelectric cartilage‐decellularized extracellular matrix (dECM) and modified gelatin (Gel‐PC). The piezoelectricity the achieved through modification diphenylalanine (FF) assembly on pore surface, while conductive properties are by incorporating poly(3,4‐ethylenedioxythiophene). In vitro experiments demonstrate that bone marrow mesenchymal stem cells (BMSCs) undergo biphasic division during differentiation. vivo studies using Parma pig model osteochondral defects exhibits superior reparative efficacy. Notably, generation electrical stimulation linked to joint movement. During activity, mechanical forces compress scaffold, leading deformation subsequent an electric potential difference. positive charges accumulated upper layer attract BMSCs, promoting their migration chondrogenic Meanwhile, negative in lower induce osteogenic differentiation BMSCs. Overall, this piezoelectric‐conducive provides promising platform effective repair defects.

Language: Английский

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Injectable, High Specific Surface Area Cryogel Microscaffolds Integrated with Osteoinductive Bioceramic Fibers for Enhanced Bone Regeneration

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(17), P. 20661 - 20676

Published: April 21, 2023

Organic-inorganic composites with high specific surface area and osteoinductivity provide a suitable microenvironment for cell ingrowth effective ossification, which could greatly promote bone regeneration. Here, we report gelatin methacryloyl (GelMA) cryogel microspheres that are reinforced hydroxyapatite (HA) nanowires calcium silicate (CS) nanofibers to achieve the goal. The prepared composite open porous structure rough facilitate anchoring, simultaneously exhibiting excellent injectability. Compared only HA- or CS-containing counterparts, GelMA composited HA:CS (termed as GMHC) sustained release of bioactive Ca, P, Si elements, conducive osteogenic differentiation marrow mesenchymal stromal cells (BMSCs). These can prevent from forming peralkalic conditions, is beneficial growth. After injection into rat calvarial defects, neo-bone tissue grows their pores, showing tight integration. embedded bioceramic components significantly regeneration, GMHC achieving best regenerative outcomes. Promisingly, organic-inorganic microspheres, area, biodegradability, osteoinductivity, act injectable microscaffolds repair defects enhanced efficiency, may widen scaffold strategy engineering.

Language: Английский

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Organic–inorganic composite hydrogels: compositions, properties, and applications in regenerative medicine

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(5), P. 1079 - 1114

Published: Jan. 1, 2024

Hydrogels, formed from crosslinked hydrophilic macromolecules, provide a three-dimensional microenvironment that mimics the extracellular matrix. They served as scaffold materials in regenerative medicine with an ever-growing demand. However, hydrogels composed of only organic components may not fully meet performance and functionalization requirements for various tissue defects. Composite hydrogels, containing inorganic components, have attracted tremendous attention due to their unique compositions properties. Rigid particles, rods, fibers,

Language: Английский

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Ion‐Engineered Microcryogels via Osteogenesis‐Angiogenesis Coupling and Inflammation Reversing Augment Vascularized Bone Regeneration

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(34)

Published: March 10, 2024

Abstract Native bone inherently requires a balanced ionic microenvironment to maintain homeostasis. Hence, scaffolds designed for the sustained release of therapeutic ions into defects hold great promise regeneration. Magnesium (Mg) and silicon (Si) are essential elements, which play crucial roles in process regeneration, impacting immunomodulation, angiogenesis, osteogenesis. Herein, porous cryogel‐type organic–inorganic composite microspheres developed as injectable microscaffolds (denoted GMN). GMN enables Mg/Si at an optimized ratio, achieving most significant synergistic effect on vascularized Various conditioned media obtained explore angiogenesis‐osteogenesis coupling, well crosstalk between marrow mesenchymal stromal cells (BMSCs) macrophages. Meanwhile, autocrine paracrine effects simultaneously modulating functions determining cell fates under guidance biofactors secreted by cells. Overall, ion‐engineering create conducive efficiently augment regeneration tissue vivo, offering versatile platform engineering.

Language: Английский

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Tough Polyurethane Hydrogels with a Multiple Hydrogen‐Bond Interlocked Bicontinuous Phase Structure Prepared by In Situ Water‐Induced Microphase Separation

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Hydrogels with mechanical performances similar to load‐bearing tissues are in demand for vivo applications. In this work, inspired by the self‐assembly behavior of amphiphilic polymers, polyurethane‐based tough hydrogels a multiple hydrogen‐bond interlocked bicontinuous phase structure through situ water‐induced microphase separation strategy developed, which poly(ethylene glycol)‐based polyurethane (PEG‐PU, hydrophilic) and poly(ε‐caprolactone)‐based (PCL‐PU, hydrophobic) blended form dry films followed water swelling. A hydrogen bonding factor, imidazolidinyl urea, is introduced into synthesis two polyurethanes, formation bonds between PEG‐PU PCL‐PU can promote homogeneous construction structures hydrogel network, features break strength 12.9 MPa, fracture energy 2435 J m −2 , toughness 48.2 MJ −3 . As biomedical patch, outstanding withstand abdominal pressure prevent hernia wall defect model. Compared commercial PP mesh, tissue/organ adhesion reduce inflammatory responses angiogenesis, thereby accelerating repair defects. This work may provide useful inspiration researchers design different gel materials solvent‐induced separation.

Language: Английский

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Enhanced osteochondral regeneration with a 3D-Printed biomimetic scaffold featuring a calcified interfacial layer

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 317 - 329

Published: March 8, 2024

The integrative regeneration of both articular cartilage and subchondral bone remains an unmet clinical need due to the difficulties mimicking spatial complexity in native osteochondral tissues for artificial implants. Layer-by-layer fabrication strategies, such as 3D printing, have emerged a promising technology replicating stratified zonal architecture varying microstructures mechanical properties. However, dynamic circulating physiological environments, mass transportation or cell migration, usually distort pre-confined biological properties layered implants, leading undistinguished variations subsequently inefficient regenerations. This study introduced biomimetic calcified interfacial layer into scaffold compact barrier between facilitate osteogenic–chondrogenic repair. consisting polycaprolactone (PCL), nano-hydroxyapatite, tasquinimod (TA) can physically biologically separate (TA-mixed, chondrocytes-load gelatin methacrylate) from bond (porous PCL). introduction preserved as-designed independent environment each regeneration, successfully inhibiting vascular invasion preventing hyaluronic calcification owing devascularization TA. improved was validated through gross examination, micro-computed tomography (micro-CT), histological immunohistochemical analyses based on vivo rat model. Moreover, gene protein expression studies identified key role Caveolin (CAV-1) promoting angiogenesis Wnt/β-catenin pathway indicated that TA blocked by CAV-1.

Language: Английский

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