Bioinspired porous three-coordinated single-atom Fe nanozyme with oxidase-like activity for tumor visual identification via glutathione

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 6, 2023

Inspired by structures of natural metalloenzymes, a biomimetic synthetic strategy is developed for scalable synthesis porous Fe-N3 single atom nanozymes (pFeSAN) using hemoglobin as Fe-source and template. pFeSAN delivers 3.3- 8791-fold higher oxidase-like activity than Fe-N4 Fe3O4 nanozymes. The high catalytic performance attributed to (1) the suppressed aggregation atomically dispersed Fe; (2) facilitated mass transfer maximized exposure active sites created mesopores thermal removal (2 ~ 3 nm); (3) unique electronic configuration oxygen-to-water oxidation pathway (analogy with cytochrome c oxidase). successfully demonstrated rapid colorimetric detection glutathione low limit (2.4 nM) wide range (50 nM-1 mM), further real-time, facile, (~6 min) precise visualization analysis methodology tumors via level, showing its potentials diagnostic clinic applications.

Language: Английский

---

Nanozymes for nanohealthcare

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: May 30, 2024

Language: Английский

---

Designing nanozymes for in vivo applications

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(10), P. 849 - 868

Published: July 18, 2024

Language: Английский

---

Deep Insight of Design, Mechanism, and Cancer Theranostic Strategy of Nanozymes

Nano-Micro Letters, Journal Year: 2023, Volume and Issue: 16(1)

Published: Nov. 21, 2023

Since the discovery of enzyme-like activity Fe

Language: Английский

---

Regulating the H₂O₂ Activation Pathway on a Well-Defined CeO₂ Nanozyme Allows the Entire Steering of Its Specificity between Associated Enzymatic Reactions

ACS Nano, Journal Year: 2023, Volume and Issue: 17(17), P. 17383 - 17393

Published: Aug. 14, 2023

Nanozymes are promising alternatives to natural enzymes, but their use remains limited owing poor specificity. For example, CeO2 activates H2O2 and displays peroxidase (POD)-like, catalase (CAT)-like, haloperoxidase (HPO)-like activities. Since they unavoidably compete for H2O2, affecting its utilization in the target application, precise manipulation of reaction specificity is thus imperative. Herein, we showed that one can simply achieve this by manipulating activation pathway on pristine well-defined shapes. This because coordination electronic structures Ce sites vary with surfaces, wherein (100) (111) surfaces display nearly 100% toward POD-/CAT-like HPO-like activities, respectively. The antibacterial results suggest latter surface well-utilize kill bacteria (cf., former), which anti-biofouling applications. work provides atomic insights into synthesis nanozymes improved activity, specificity, utilization.

Language: Английский

---

Transition-Metal-Based Nanozymes: Synthesis, Mechanisms of Therapeutic Action, and Applications in Cancer Treatment

ACS Nano, Journal Year: 2024, Volume and Issue: 18(19), P. 12049 - 12095

Published: May 2, 2024

Cancer, as one of the leading causes death worldwide, drives advancement cutting-edge technologies for cancer treatment. Transition-metal-based nanozymes emerge promising therapeutic nanodrugs that provide a reference therapy. In this review, we present recent breakthrough First, comprehensively outline preparation strategies involved in creating transition-metal-based nanozymes, including hydrothermal method, solvothermal chemical reduction biomimetic mineralization and sol–gel method. Subsequently, elucidate catalytic mechanisms (catalase (CAT)-like activities), peroxidase (POD)-like oxidase (OXD)-like activities) superoxide dismutase (SOD)-like along with their activity regulation such morphology control, size manipulation, modulation, composition adjustment surface modification under environmental stimulation. Furthermore, elaborate on diverse applications anticancer therapies encompassing radiotherapy (RT), chemodynamic therapy (CDT), photodynamic (PDT), photothermal (PTT), sonodynamic (SDT), immunotherapy, synergistic Finally, challenges faced by are discussed alongside future research directions. The purpose review is to offer scientific guidance will enhance clinical based transition metals.

Language: Английский

---

N-Doped Carbon Nanotubes Supported Fe–Mn Dual-Single-Atoms Nanozyme with Synergistically Enhanced Peroxidase Activity for Sensitive Colorimetric Detection of Acetylcholinesterase and Its Inhibitor

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(22), P. 8640 - 8648

Published: May 23, 2023

Monitoring acetylcholinesterase (AChE) and its inhibitors is of importance for early diagnosis therapy neurological diseases. Herein, N-doped carbon nanotubes supported Fe–Mn dual-single-atoms (FeMn DSAs/N-CNTs) were fabricated by a simple pyrolysis, as thoroughly figured out series the characterization techniques. The peroxidase-like activity FeMn DSAs/N-CNTs was investigated catalytic oxidation 3,3′,5,5′-tetramethylbenzidine (TMB) to generate rich hydroxyl radicals (·OH) in H2O2 system, which effectively catalyzed colorless TMB blue oxidized (ox-TMB). Besides, greatly weakened thiocholine (derived from AChE), accompanied making ox-TMB fade. Impressively, highly improved property further evidenced density functional theory (DFT) calculations, where dual-single atoms show lower energy barrier (0.079 eV) their interactions with N-CNTs played critical roles producing oxygen radicals. By virtue nanozyme, low-cost, specific, sensitive colorimetric sensor built detection AChE broader linear range 0.1–30 U L–1 limit (LOD, 0.066 L–1), combined feasible analysis human serum samples. Also, this platform applied measuring huperzine A inhibitor wide scope 5–500 nM LOD down 4.17 nM. This strategy provides low-cost convenient approach clinical drug development.

Language: Английский

---

Recent advances in multi‐metallic‐based nanozymes for enhanced catalytic cancer therapy

BMEMat, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 19, 2023

Abstract Nanozymes have emerged as a promising alternative to natural enzymes, effectively addressing enzymes' inherent limitation. Versatility and potential applications of nanozyme span across various fields, with catalytic tumor therapy being one prominent area. This has sparked significant interest exploration in the utilization nanozymes for targeted cancer treatment. Recent advancements interdisciplinary research, nanotechnology, biotechnology, technology led emergence multi‐metallic‐based nanozymes, which exhibit tremendous further development. review focuses on investigating synergistic effects aiming enhance our understanding their activities facilitate broader applications. We comprehensively survey remarkable achievements synthesis, mechanisms, latest therapy. Furthermore, we identify current limitations prospects development new materials application novel technologies, along challenges associated underscores significance emphasizes need continued well impact realization breakthroughs

Language: Английский

---

Optimizing the standardized assays for determining the catalytic activity and kinetics of peroxidase-like nanozymes

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(12), P. 3470 - 3488

Published: Aug. 15, 2024

Language: Английский

---

Single-Atom Nanozymes: From Precisely Engineering to Extensive Applications

Accounts of Materials Research, Journal Year: 2024, Volume and Issue: 5(3), P. 347 - 357

Published: Feb. 3, 2024

ConspectusNanozymes are nanomaterials with intrinsic enzyme-like properties that can overcome the current limitations of natural enzymes, such as high preparation cost, instability, restricted application scenarios, etc. Since Fe3O4 nanoparticles (NPs) were shown to possess peroxidase (POD)-like activity in 2007, thousands reported mimic catalytic various types enzymes including catalase (CAT), haloperoxidase, superoxide dismutase (SOD), glucose oxidase, glutathione peroxidase, hydrolase, nuclease, nitroreductase, and others. Particularly, some nanozymes showed multienzyme-like activities regarding changes scenarios temperature, pH, Benefiting from their distinct physical-chemical characteristics properties, have been widely applied biomedical related fields vitro detections vivo therapeutic treatments. However, currently ambiguous structure–function correlations relatively inferior compared promote extensive efforts for modifications on development novel alternative nanozymes. The single-atom (SAzymes) present a unique way highly evolved enzyme active centers, because atomically dispersed sites, which leads atom utilization efficiency and, thus, potentially extraordinary activity. Also, abilities modify centers and/or tune interactions between metal supporting ligands provide precise engineer SAzymes at atomic levels. Given well-defined geometric electronic structures, thus serve exceptional templates deciphering relationships, is beneficial further improving performances.In this Account, we will review our recent other notable works developments effective mimics applications areas. We begin brief introduction why emergence SAzymes, artificial enzyme, tackles challenges facing. Next, focus systematic design, synthesis optimization especially impacts engineering environment an enzymologist perspective. For example, alternations first-shell ligand N P/S, SAzymes' CAT-like increased more than 4-fold. coordination numbers (x) Co–Nx(C) SAzyme significantly altered its oxidase (OXD)-like kinetics Then, discuss ways standardization specific kinetics. also wide ranges colorimetric biologicals, antibiosis treatments, cancer therapies. Finally, address future perspectives

Language: Английский

---