Light-based vat-polymerization bioprinting

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: June 22, 2023

Language: Английский

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Intelligent Vascularized 3D/4D/5D/6D-Printed Tissue Scaffolds

Nano-Micro Letters, Journal Year: 2023, Volume and Issue: 15(1)

Published: Oct. 31, 2023

Abstract Blood vessels are essential for nutrient and oxygen delivery waste removal. Scaffold-repairing materials with functional vascular networks widely used in bone tissue engineering. Additive manufacturing is a technology that creates three-dimensional solids by stacking substances layer layer, mainly including but not limited to 3D printing, also 4D 5D printing 6D printing. It can be effectively combined vascularization meet the needs of vascularized scaffolds precisely tuning mechanical structure biological properties smart scaffolds. Herein, development neovascularization engineering systematically discussed terms importance tissue. Additionally, research progress future prospects printed scaffold highlighted presented four categories: scaffolds, cell-based loaded specific carriers bionic Finally, brief review additive manufacturing-tissue related tissues such as engineering, cardiovascular system, skeletal muscle, soft discussion challenges efforts leading significant advances intelligent regeneration presented.

Language: Английский

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Multi‐Material Volumetric Additive Manufacturing of Hydrogels using Gelatin as a Sacrificial Network and 3D Suspension Bath

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(34)

Published: Jan. 20, 2024

Abstract Volumetric additive manufacturing (VAM) is an emerging layerless method for the rapid processing of reactive resins into 3D structures, where printing much faster (seconds) than other lithography and direct ink writing methods (minutes to hours). As a vial resin rotates in VAM process, patterned light exposure defines object then that has not undergone gelation can be washed away. Despite promise VAM, there are challenges with soft hydrogel materials from non‐viscous precursors, including multi‐material constructs. To address this, sacrificial gelatin used modulate viscosity support cytocompatible macromers based on poly(ethylene glycol) (PEG), hyaluronic acid (HA), polyacrylamide (PA). After printing, removed by washing at elevated temperature. print constructs, gelatin‐containing as shear‐yielding suspension bath (including HA further properties) extruded define processed defined object. Multi‐material constructs methacrylated (MeHA) methacrylamide (GelMA) printed (as proof‐of‐concept) encapsulated mesenchymal stromal cells (MSCs), local properties guide cell spreading behavior culture.

Language: Английский

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Materials and Strategies to Enhance Melt Electrowriting Potential

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(24)

Published: March 6, 2024

Melt electrowriting (MEW) is an emerging additive manufacturing (AM) technology that enables the precise deposition of continuous polymeric microfibers, allowing for creation high-resolution constructs. In recent years, MEW has undergone a revolution, with introduction active properties or additional functionalities through novel polymer processing strategies, incorporation functional fillers, postprocessing, combination other techniques. While extensively explored in biomedical applications, MEW's potential fields remains untapped. Thus, this review explores characteristics from materials science perspective, emphasizing diverse range and composites processed by technique their current applications. Additionally, prospects offered postprinting techniques are explored, together synergy achieved combining melt methods. By highlighting untapped potentials MEW, aims to inspire research groups across various leverage innovative endeavors.

Language: Английский

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Holographic tomographic volumetric additive manufacturing

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 11, 2025

Language: Английский

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A review of materials used in tomographic volumetric additive manufacturing

MRS Communications, Journal Year: 2023, Volume and Issue: 13(5), P. 764 - 785

Published: Aug. 29, 2023

Volumetric additive manufacturing is a novel fabrication method allowing rapid, freeform, layer-less 3D printing. Analogous to computer tomography (CT), the projects dynamic light patterns into rotating vat of photosensitive resin. These build up three-dimensional energy dose within resin, solidifying volume desired object seconds. Departing from established sequential methods like stereolithography or digital printing, volumetric offers new opportunities for materials that can be used include viscous acrylates and elastomers, epoxies (and orthogonal epoxy-acrylate formulations with spatially controlled stiffness) formulations, tunable stiffness thiol-enes shape memory foams, polymer derived ceramics, silica-nanocomposite based glass, gelatin-based hydrogels cell-laden biofabrication. Here we review these materials, highlight challenges adapt them manufacturing, discuss perspectives they present.

Language: Английский

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3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(23)

Published: June 23, 2023

Abstract 3D bioprinting has developed tremendously in the last couple of years and enables fabrication simple, as well complex, tissue models. The international space agencies have recognized unique opportunities these technologies for manufacturing cell models basic research space, particular investigating effects microgravity cosmic radiation on different types human tissues. In addition, is capable producing clinically applicable grafts, its implementation therefore can support autonomous medical treatment options astronauts future long term far‐distant missions. article discusses but also challenges operating bioprinters under conditions, mainly microgravity. While some process steps, most which involving handling liquids, are challenging microgravity, this environment help overcome problems such sedimentation low viscous bioinks. Hopefully, publication will motivate more researchers to engage topic, with publicly available becoming at International Space Station (ISS) imminent future.

Language: Английский

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Advances in volumetric bioprinting

Biofabrication, Journal Year: 2023, Volume and Issue: 16(1), P. 012004 - 012004

Published: Nov. 3, 2023

The three-dimensional (3D) bioprinting technologies are suitable for biomedical applications owing to their ability manufacture complex and high-precision tissue constructs. However, the slow printing speed of current layer-by-layer (bio)printing modality is major limitation in biofabrication field. To overcome this issue, volumetric (VBP) developed. VBP changes layer-wise operation conventional devices, permitting creation geometrically complex, centimeter-scale constructs tens seconds. next step onward from sequential methods, opening new avenues fast additive manufacturing fields engineering, regenerative medicine, personalized drug testing, soft robotics, etc. Therefore, review introduces principles hardware designs VBP-based techniques; then focuses on recent advances (bio)inks applications. Lastly, limitations discussed together with future direction research.

Language: Английский

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Materials Design Innovations in Optimizing Cellular Behavior on Melt Electrowritten (MEW) Scaffolds

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(18)

Published: Jan. 21, 2024

Abstract The field of melt electrowriting (MEW) has seen significant progress, bringing innovative advancements to the fabrication biomaterial scaffolds, and creating new possibilities for applications in tissue engineering beyond. Multidisciplinary collaboration across materials science, computational modeling, AI, bioprinting, microfluidics, dynamic culture systems offers promising opportunities gain deeper insights into complex biological systems. As focus shifts towards personalized medicine reduced reliance on animal models, multidisciplinary approach becomes indispensable. This review provides a concise overview current strategies innovations controlling optimizing cellular responses MEW highlighting potential scaffold material, architecture, modeling tools accelerate development efficient biomimetic Innovations material science incorporation biologics scaffolds have shown great adding complexity engineered These techniques pave way exciting regeneration, drug screening, cell therapies.

Language: Английский

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Dynamic interface printing

Nature, Journal Year: 2024, Volume and Issue: 634(8036), P. 1096 - 1102

Published: Oct. 30, 2024

Additive manufacturing is an expanding multidisciplinary field encompassing applications including medical devices1, aerospace components2, microfabrication strategies3,4 and artificial organs5. Among additive approaches, light-based printing technologies, two-photon polymerization6, projection micro stereolithography7,8 volumetric printing9–14, have garnered significant attention due to their speed, resolution or potential for biofabrication. Here we introduce dynamic interface printing, a new 3D approach that leverages acoustically modulated, constrained air–liquid boundary rapidly generate centimetre-scale structures within tens of seconds. Unlike this process eliminates the need intricate feedback systems, specialized chemistry complex optics while maintaining rapid speeds. We demonstrate versatility technique across broad array materials geometries, those would be impossible print with conventional layer-by-layer methods. In doing so, fabrication in situ, overprinting, structural parallelization biofabrication utility. Moreover, show formation surface waves at enables enhanced mass transport, improves material flexibility permits particle patterning. We, therefore, anticipate will invaluable where high-resolution, scalable throughput biocompatible required. Dynamic form

Language: Английский

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