Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 26, 2024
Language: Английский
Nano Select, Journal Year: 2025, Volume and Issue: unknown
Published: March 4, 2025
ABSTRACT To expand the use of collagen‐based biomaterials beyond their current applications in three‐dimensional (3D) cell culture, tissue engineering, and biofabrication, limitations such as poor shear‐thinning behavior control over porosity during gelation need to be overcome. Granular promise address these constraints, however uniform scalable preparation from extracellular matrix materials is challenging. this need, we employed a droplet microfluidic approach prepared irregularly shaped microgels fibrillar collagen collagen‐glycosaminoglycan (GAG) copolymer continuous oil phase, at rates up 5500 s −1 . The allowed us tune average microgel size 40 170 µm. Microgels obtained after removal phase were found promote attachment proliferation human fibroblasts mesenchymal stromal/stem cells. with packing densities exceeding 65 vol% exhibited rheological behavior, requirement for injectable bioinks. Cell‐containing granular contracted 2.8 times less than thermally gelled matrices comparable concentration. In case study, skin model fibroblast containing collagen‐GAG (CG) layer covered an epithelium revealed immunohistochemical markers associated intact month‐long air–liquid interface (ALI) culture.
Language: Английский
Citations
1
Materials Today Bio, Journal Year: 2023, Volume and Issue: 23, P. 100846 - 100846
Published: Oct. 28, 2023
3D bioprinting technology is widely used to fabricate various tissue structures. However, the absence of vessels hampers ability bioprinted tissues receive oxygen and nutrients as well remove wastes, leading a significant reduction in their survival rate. Despite advancements bioinks technologies, vascular structures continue be unsuitable for transplantation compared natural blood vessels. In addition, complete assessment index system evaluating structure function vitro has not yet been established. Therefore, this review, we firstly highlight significance selecting suitable techniques they two synergize with each other. Subsequently, focusing on both vascular-associated cells tissues, provide relatively thorough functions based physiological that possess. We end review applications models, such vessel-on-a-chip, simulating pathological processes conducting drug screening at organ level. believe development fully functional will soon make great contributions engineering regenerative medicine.
Language: Английский
Citations
16
Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(16), P. 5462 - 5473
Published: Jan. 1, 2023
This minireview highlights recent advances on the application of embedded 3D bioprinting concepts for fabrication in vitro models.
Language: Английский
Citations
14
Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(10), P. 1241 - 1257
Published: April 23, 2024
Advances in tissue engineering for both system modeling and organ regeneration depend on embracing recapitulating the target tissue's functional structural complexity. Microenvironmental features such as anisotropy, heterogeneity, other biochemical mechanical spatiotemporal cues are essential regulating development function. Novel biofabrication strategies innovative biomaterial design have emerged promising tools to better reproduce features. These facilitate a transition towards high-fidelity biomimetic structures, offering opportunities deeper understanding of function superior therapies. In this review, we explore some key compositional aspects tissues, lay out how achieve similar outcomes with current fabrication strategies, identify main challenges avenues future research.
Language: Английский
Citations
6
Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 26, 2024
Language: Английский
Citations
6