Enhancing Immunomodulation and Osseointegration of Bone Implants via Thrombin‐Activated Platelet‐Rich Plasma Self‐Assembly DOI Open Access
Xiaotong Shi, Zongliang Wang, Zhiguo Bi

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Platelet‐rich plasma (PRP) is characterized by elevated concentrations of growth factors that facilitate bone repair. Nonetheless, the effective integration PRP with implants and sustained release its active constituents pose significant challenges. In this study, thrombin grafted onto surface polyetheretherketone (PEEK) via an N,N'‐Disuccinimidyl Carbonate (DSC) linker retained enzymatic activity enables controlled activation self‐assembly, resulting in formation a functional bio‐gel layer. The optimal concentration to be 100 U/ mL −1 determined, at which point both grafting amount reaches their peak, no further increases observed higher concentrations. solutions varying platelet enrichment ratios are subsequently activated on thrombin‐grafted PEEK surface, yielding self‐assembled bio‐gels capable factor for up 16 days. thrombin‐activated not only enhances vitro cell adhesion, proliferation, osteogenic differentiation, vascularization specific polarization macrophages, but also effectively facilitates vivo angiogenesis, immunomodulation dose‐dependent manner. Consequently, gel presents promising strategy biological functionalization orthopedic applications.

Language: Английский

Asymmetric adhesive SIS-based wound dressings for therapeutically targeting wound repair DOI Creative Commons

Wende Yao,

Zelong Song, Xiaodong Ma

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 19, 2024

Abstract Severe tissue injuries pose a significant risk to human health. Conventional wound dressings fall short in achieving effective regeneration, resulting suboptimal postoperative healing outcomes. In this study, an asymmetric adhesive dressing (marked as SIS/PAA/LAP) was developed, originating from acrylate acid (AA) solution with laponite (LAP) nanoparticles polymerization and photo-crosslinked on the decellularized extracellular matrix small intestinal submucosa (SIS) patch. Extensive studies demonstrated that SIS/PAA/LAP exhibited higher adhesion strength (~ 33 kPa) burst 22 compared conventional like Tegaderm products. Importantly, it maintained favorable cell viability robust angiogenic capacity. animal models of full-thickness skin rats Bama miniature pigs, could be precisely applied sites. By accelerating formation vascularization, displayed superior repair This asymmetrically SIS-based patch would hold promising applications field dressings.

Language: Английский

Citations

10
Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair DOI
Qiong Wu,

Yayuan Guo,

Hongwei Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130843 - 130843

Published: March 12, 2024

Language: Английский

Citations

10
Immunomodulation effects of collagen hydrogel encapsulating extracellular vesicles derived from calcium silicate stimulated-adipose mesenchymal stem cells for diabetic healing DOI Creative Commons

Yen-Hong Lin,

Yeh Chen,

En‐Wei Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 27, 2025

Diabetic wounds are characterized by chronic inflammation, reduced angiogenesis, and insufficient collagen deposition, leading to impaired healing. Extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ADSC) offer a promising cell-free therapeutic strategy, yet their efficacy immunomodulation can be enhanced through bioactivation. In this study, we developed calcium silicate (CS)-stimulated ADSC-derived EVs (CSEV) incorporated into hydrogels create sustained-release system for promoting diabetic wound CSEV exhibited protein content, surface marker expression, bioactive cargo enriched with pro-angiogenic anti-inflammatory factors. vitro, CSEV-loaded significantly reactive oxygen species production, promoted cell proliferation migration compared standard EV-loaded collagen. Cytokine profiling revealed the upregulation of cytokines extracellular matrix components, highlighting immunomodulatory regenerative potential. vivo, histological evaluation rabbit models treated superior reepithelialization organized indicating accelerated closure. These findings underscore potential as an innovative effective platform enhancing healing simultaneously addressing inflammation tissue regeneration.

Language: Английский

Citations

1
Protein-polysaccharide based nanogel/hydrogel composite with controlled continuous delivery of drug for enhanced wound healing DOI
Yi Mao, Bing Tian Zhao,

Linghui Xu

et al.

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: unknown, P. 123407 - 123407

Published: Feb. 1, 2025

Language: Английский

Citations

1
Mesenchymal stem cells and mesenchymal stem cell-derived exosomes: a promising strategy for treating retinal degenerative diseases DOI Creative Commons

Wenjing An,

Wenliang Zhang, Qi Jia

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 21, 2025

Abstract Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic strategy in regenerative medicine, demonstrating significant potential for clinical applications. Evidence suggests that MSCs not only exhibit multipotent differentiation but also exert critical effects retinal degenerative diseases via robust paracrine mechanisms. protect from damage by modulating inflammation, inhibiting apoptosis, alleviating oxidative stress, and suppressing cell death pathways. Furthermore, contribute to structural functional stability facilitating vascular remodeling donating mitochondria cells. Of particular interest, MSC-derived exosomes gained widespread attention compelling cell-free therapy. Owing their potent anti-inflammatory, anti-apoptotic, vascular-stabilizing properties, show promise the treatment of diseases.

Language: Английский

Citations

1
A thermally stable bioactive chitosan scaffold with pH-responsive exosome adsorption and release function promotes wound healing DOI Creative Commons

Jifang Yuan,

Meirong Li, Xiaofeng He

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141552 - 141552

Published: Feb. 1, 2025

Language: Английский

Citations

1
Exosomes in Precision Oncology and Beyond: From Bench to Bedside in Diagnostics and Therapeutics DOI Open Access

Emile M. Youssef,

Dannelle Palmer,

Brandon Fletcher

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(6), P. 940 - 940

Published: March 10, 2025

Exosomes have emerged as pivotal players in precision oncology, offering innovative solutions to longstanding challenges such metastasis, therapeutic resistance, and immune evasion. These nanoscale extracellular vesicles facilitate intercellular communication by transferring bioactive molecules that mirror the biological state of their parent cells, positioning them transformative tools for cancer diagnostics therapeutics. Recent advancements exosome engineering, artificial intelligence (AI)-driven analytics, isolation technologies are breaking barriers scalability, reproducibility, clinical application. Bioengineered exosomes being leveraged CRISPR-Cas9 delivery, while AI models enhancing biomarker discovery liquid biopsy accuracy. Despite these advancements, key obstacles heterogeneity populations lack standardized protocols persist. This review synthesizes pioneering research on biology, molecular translation, emphasizing dual roles both mediators tumor progression intervention. It also explores emerging areas, including microbiome–exosome interactions integration machine learning exosome-based medicine. By bridging innovation with translational strategies, this work charts a forward-looking path integrating into next-generation care, setting it apart comprehensive guide overcoming technological hurdles rapidly evolving field.

Language: Английский

Citations

1
A bio-degradable, triboelectric self-stimulated herbal transdermal therapeutic dressing for healing massive chronic wound DOI
Hui Liu, Ming Zhang, Lulu Zu

et al.

Nano Energy, Journal Year: 2024, Volume and Issue: 125, P. 109585 - 109585

Published: April 7, 2024

Language: Английский

Citations

8
Different origin-derived exosomes and their clinical advantages in cancer therapy DOI Creative Commons
Xiaoyan Jin, Jing Zhang, Yufu Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Exosomes, as a class of small extracellular vesicles closely related to the biological behavior various types tumors, are currently attracting research attention in cancer diagnosis and treatment. Regarding diagnosis, stability their membrane structure wide distribution body fluids render exosomes promising biomarkers. It is expected that exosome-based liquid biopsy will become an important tool for tumor future. For treatment, exosomes, “golden communicators” between cells, can be designed deliver different drugs, aiming achieve low-toxicity low-immunogenicity targeted delivery. Signaling pathways exosome contents also used safer more effective immunotherapy against tumors. Exosomes derived from range sources, exhibit characteristics well clinical application advantages therapies. In this review, we analyzed main sources have great potential broad prospects therapy. Moreover, compared therapeutic advantages, providing new ideas exosomes.

Language: Английский

Citations

8
Smart Microneedle Arrays Integrating Cell‐Free Therapy and Nanocatalysis to Treat Liver Fibrosis DOI Creative Commons
Yanteng Xu, Yixin Zhang, Hao Tian

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)

Published: June 14, 2024

Abstract Liver fibrosis is a chronic pathological condition lacking specific clinical treatments. Stem cells, with notable potential in regenerative medicine, offer promise treating liver fibrosis. However, stem cell therapy hindered by immunological rejection, carcinogenesis risk, efficacy variation, and high cost. secretome‐based cell‐free offers solutions to address these challenges, but it limited low delivery efficiency rapid clearance. Herein, an innovative approach for situ implantation of smart microneedle (MN) arrays enabling precisely controlled multiple therapeutic agents directly into fibrotic tissues developed. By integrating platinum‐based nanocatalytic combination therapy, the MN can deactivate hepatic stellate cells. Moreover, they promote excessive extracellular matrix degradation more than 75%, approaching normal levels. Additionally, provide hepatocyte protection while reducing inflammation levels ≈70–90%. They also exhibit remarkable capability scavenging almost 100% reactive oxygen species alleviating hypoxia. Ultimately, this treatment strategy effectively restrain progression. The comprehensive vitro vivo experiments, supplemented proteome transcriptome analyses, substantiate effectiveness fibrosis, holding immense applications.

Language: Английский

Citations

6