Induction of MASH in three-dimensional bioprinted human liver tissue DOI Creative Commons
Viral Joshi,

Dwayne Carter,

Alice E. Chen

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0312615 - e0312615

Published: Dec. 30, 2024

Metabolic dysfunction-associated steatohepatitis (MASH), formerly known as nonalcoholic is a major risk factor for cirrhosis and hepatocellular carcinoma (HCC) leading cause of liver transplantation. MASH caused by an accumulation toxic fat molecules in the hepatocyte which leads to inflammation fibrosis. Inadequate human “MASH dish” models have limited our advances understanding pathogenesis drug discovery. This study uses complex multicellular 3D bioprinting, combining hepatocytes with nonparenchymal cells physiologically relevant cell ratios using biocompatible hydrogels generate bioinks Bioprinted tissues consisting four types, (hepatocytes, endothelial cells, Kupffer hepatic stellate cells) are generated from purified normal livers, this bioprinting platform. These incubated cocktail fatty acids, lipopolysaccharide (LPS), fructose produce phenotype comparison control media. Furthermore, these bioprinted sufficient size undergo histological processing immunohistchemistry comparable classic clinical pathological analysis. The develop steatosis, inflammation, fibrosis, response induction Additionally, transcriptome differed significantly healthy more closely resembled biopsies livers patients Thus, has developed tissue suitable studies on pathophysiology

Language: Английский

Recent advances in Organ-on-a-Chip models: How precision engineering integrates cutting edge technologies in fabrication and characterization DOI
Sorour Sadeghzade, Mohammad Hosseini Hooshiar, Hossein Akbari

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 38, P. 102231 - 102231

Published: May 14, 2024

Language: Английский

Citations

7
Vascularized tumor models for the evaluation of drug delivery systems: a paradigm shift DOI Creative Commons
Elliot Lopez, Claire Wilhelm, Teresa Simón‐Yarza

et al.

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: 14(8), P. 2216 - 2241

Published: April 15, 2024

Abstract As the conversion rate of preclinical studies for cancer treatment is low, user-friendly models that mimic pathological microenvironment and drug intake with high throughput are scarce. Animal key, but an alternative to reduce their use would be valuable. Vascularized tumor-on-chip combine great versatility scalable easy use. Several strategies integrate both tumor vascular compartments have been developed, few used assess delivery. Permeability, intra/extravasation, free circulation often evaluated, imperfectly recapitulate processes at stake. Indeed, targeting chemoresistance bypass must investigated design promising therapeutics. In vitro help development delivery systems (DDS) thus needed. They allow selecting good candidates before animal based on rational criteria such as accumulation, diffusion in tumor, potency, well absence side damage. this review, we focus vascularized models. First, detail fabrication, especially materials, cell types, coculture used. Then, different vascularization described along classical applications intra/extravasation or assessment. Finally, current trends DDS discussed overview efforts domain. Graphical

Language: Английский

Citations

6
In Vivo and In Vitro Models of Hepatic Fibrosis for Pharmacodynamic Evaluation and Pathology Exploration DOI Open Access
Yanting Hu, Zhongrui Zhang,

Akida Adiham

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 696 - 696

Published: Jan. 15, 2025

Hepatic fibrosis (HF) is an important pathological state in the progression of chronic liver disease to end-stage and usually triggered by alcohol, nonalcoholic fatty liver, hepatitis viruses, autoimmune (AIH), or cholestatic disease. Research on novel therapies has become a hot topic due reversibility HF. into molecular mechanisms pathology HF potential drug screening relies reliable rational biological models, mainly including animals cells. Hence, number modeling approaches have been attempted based human dietary, pathological, physiological factors development In this review, classical methods last 10 years were collected from electronic databases, Web Science, PubMed, ScienceDirect, ResearchGate, Baidu Scholar, CNKI. Animal models are induced chemical toxicants, special diets, pathogenic microorganisms, surgical operations, gene editing. The advantages limitations hepatic stellate cells (HSCs), organoids, 3D coculture-based established vitro also proposed summarized. This information provides scientific basis for discovery mechanism treatment

Language: Английский

Citations

0
Dressed in Collagen: 2D and 3D Cardiac Fibrosis Models DOI Open Access
Maria Cardona-Timoner, Rita N. Gomes, Diana S. Nascimento

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3038 - 3038

Published: March 26, 2025

Cardiovascular diseases (CVD), the leading cause of death worldwide, and their strong association with fibrosis highlight pressing need for innovative antifibrotic therapies. In vitro models have emerged as valuable tools replicating cardiac 'in a dish', facilitating study disease mechanisms serving platforms drug testing development. These in systems encompass 2D 3D models, each its own limitations advantages. offer high reproducibility, cost-effectiveness, high-throughput capabilities, but they oversimplify complex fibrotic environment. On other hand, provide greater biological relevance are more complex, harder to reproduce, less suited screening. The choice model depends on specific research question stage Despite significant progress, challenges remain, including integration immune cells optimizing scalability throughput highly biomimetic systems. Herein, we review recent focus shared characteristics remaining challenges, explore how organs could inspire novel approaches research, showcasing potential strategies that be adapted refine myocardial models.

Language: Английский

Citations

0
Organoids in Dynamic Culture: Microfluidics and 3D Printing Technologies DOI
Xin Su, Mingqi Wang,

Ruqiang Yuan

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

With the rapid advancement of biomaterials and tissue engineering technologies, organoid research its applications have made significant strides. Organoids are increasingly utilized in pharmacology, regenerative medicine, precision clinical medicine. Current trends moving toward multifunctional composite three-dimensional cultivation dynamic strategies. Key technologies driving this evolution, including 3D printing microfluidics, continue to impact new areas discovery relevance. This review provides a systematic overview these emerging trends, discussing strengths limitations critical offering insight directions for professionals working field.

Language: Английский

Citations

0
Recent advances of nanomaterials in imaging liver fibrosis DOI Creative Commons
Jin Cui, Shuxuan Zhang,

Xueli Xu

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Abstract Liver fibrosis is a pathological process resulting from prolonged exposure to various injury factors. It characterized by the abnormal proliferation and activation of hepatic stellate cells excessive deposition extracellular matrix. If left untreated, it can progress cirrhosis, liver failure, even cancer. There currently no efficient accurate clinical diagnostic method for early fibrosis. Therefore, there an urgent need address challenge staging diagnosis in practice. Recently, nanomaterials have demonstrated significant potential enhancing Nanomaterials possess ability precisely identify target microenvironment associated with By their enrichment area, improve imaging contrast lesions liver, thereby enabling Accordingly, this review delves into latest research advancements concerning diagnosis.

Language: Английский

Citations

3
Hydrogels as carriers deliver stem cells/exosomes for liver injury DOI Creative Commons
Qiuxia Zheng, Jia Yao,

Zongbin Sun

et al.

Materials Advances, Journal Year: 2024, Volume and Issue: 5(9), P. 3587 - 3601

Published: Jan. 1, 2024

Hydrogels are biomaterials with porous structures, which have the characteristics of slow-release and mimic ECM. Hydrogel-encapsulated stem cells or exosomes can gradually release therapeutic elements enhance liver regeneration in injuries.

Language: Английский

Citations

2
A cubic Cu2O@Ag bioprobe for label-free SERS classification of hepatic fibrosis and hepatocellular carcinoma DOI

Yujiao Xie,

Xue Li,

Lei Xu

et al.

Materials Chemistry Frontiers, Journal Year: 2024, Volume and Issue: 8(18), P. 2978 - 2988

Published: Jan. 1, 2024

In this study, a cubic Cu 2 O@Ag core–shell substrate for label-free SERS was constructed and machine-learning-assisted linear discriminant analysis used identification of hepatic fibrosis hepatocellular carcinoma.

Language: Английский

Citations

1
3D bioprinting of Gelatin-Alginate bioinks for biofabrication of in vitro liver sinusoid model DOI Open Access
Ashwini Rahul Akkineni, Anja Lode, Michael Gelinsky

et al.

Procedia CIRP, Journal Year: 2024, Volume and Issue: 125, P. 96 - 100

Published: Jan. 1, 2024

Language: Английский

Citations

1
Synergistic Anticancer Strategy Targeting ECM Stiffness: Integration of Matrix Softening and Mechanical Signal Transduction Blockade in Primary Liver Cancers DOI Creative Commons
Zefeng Shen,

Liye Tao,

Yali Wang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Abstract The development of primary liver cancer (hepatocellular carcinoma [HCC] and intrahepatic cholangiocarcinoma [ICC]) is linked to its physical microenvironment, particularly extracellular matrix (ECM) stiffness. Potential anticancer strategies targeting ECM stiffness include prevention/reversal the stiffening process disruption response cells mechanical signals from ECM. However, each strategy has limitations. Therefore, authors propose integrating them maximize their strengths. Compared with HCC, ICC a stiffer worse prognosis. selected investigate mechanisms underlying influence on progression application integrated In summary, immunofluorescence results for 181 tissue chips (ICC, n = 91; 90) analysis TCGA mRNA‐sequencing demonstrate that can affect phenotypes cancers. YAP1/ABHD11‐AS1/STAU2/ZYX/p‐YAP1 pathway useful entry point exploration specific signal conduction in impact progression. Moreover, synergistic (ICCM@NPs + siABHD11‐AS1@BAPN) constructed by softening blocking intracellular transduction provide insights treatment cancers characterized stiff

Language: Английский

Citations

1