Harnessing the tumor microenvironment: targeted cancer therapies through modulation of epithelial-mesenchymal transition

Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 13, 2025

The tumor microenvironment (TME) is integral to cancer progression, impacting metastasis and treatment response. It consists of diverse cell types, extracellular matrix components, signaling molecules that interact promote growth therapeutic resistance. Elucidating the intricate interactions between cells TME crucial in understanding progression challenges. A critical process induced by epithelial-mesenchymal transition (EMT), wherein epithelial acquire mesenchymal traits, which enhance their motility invasiveness progression. By targeting various components TME, novel investigational strategies aim disrupt TME's contribution EMT, thereby improving efficacy, addressing resistance, offering a nuanced approach therapy. This review scrutinizes key players emphasizing avenues therapeutically components. Moreover, article discusses implications for resistance mechanisms highlights current toward modulation along with potential caveats.

Language: Английский

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Advanced strategies in 3D bioprinting for vascular tissue engineering and disease modelling using smart bioinks

Virtual and Physical Prototyping, Journal Year: 2024, Volume and Issue: 19(1)

Published: Aug. 30, 2024

Advanced three-dimensional (3D) bioprinting technology enables the precise production of complex vascular structures and biomimetic models, driving advancements in tissue engineering disease mechanism research. At core this is smart bioink, which suitable for fabricating models that can be vascularised to meet property requirements various tissues. Examples bioinks include decellularized extracellular matrix (dECM), photocrosslinkable, reversible, microgel-based biphasic (MB) bioinks, whose mechanical properties tuned through external stimuli. This tuning helps generate high-resolution complex-shaped networks essential cell survival functional maturation. review explores advanced 3D strategies using spatially controlled perfusable vitro emphasising reconstruction within bioprinted models. It also discusses challenges future prospects, suggesting could serve as alternatives traditional animal modelling drug screening.

Language: Английский

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A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 4, 2025

Extracellular matrix mimics are still needed to grow cancer cells in 3D environments and study their evolution vitro while precisely controlling relevant features. Most models currently use collagen, which is biomimetic but degrades quickly, or artificial polymers, can be chemically modified remain stiff. Herein we introduced a soft, non-adhesive, resistant hydrogel platform for tumor spheroid production using polysaccharide-based formulation. To ensure micro-structuring of the enable formation, printed molds consisting network 200-µm-diameter micropillars were used generate microstructured constructs that fit into multi-well plate. This was validated drug testing three cell lines (A673, MCF7 U87) 2 anticancer drugs (doxorubicin paclitaxel). Drug response assessed through bright-field microscopy monitoring viability measurements after 48 h treatment. validates pullulan-dextran hydrogels combined with situ screening.

Language: Английский

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Embolization-on-a-chip: Novel Vascularized Liver Tumor Model for Evaluation of Cellular and Cytokine Response to Embolic Agents

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

ABSTRACT Background Embolization is a well-established treatment modality for liver cancer. However, traditional embolization agents are limited by inefficient delivery and aggregation in blood vessels. Novel shear-thinning hydrogels (STH) have been developed to address the need safer more effective local of embolic therapeutics. Objective We aim evaluate efficacy novel such as STH using human-relevant vitro model that recapitulates human hepatocellular carcinoma capillary networks. Methods A vascularized liver-tumor-on-a-chip was assess agent performance. The effects drug-eluting (DESTH) on tumor cell viability, surface marker expression, vasculature morphology, cytokine responses were evaluated. To study microvasculature morphology independent chemotherapy compound, we assessed effect different drug-free vascular microenvironment under flow conditions. Results DESTH induced death, downregulated expression Epithelial Cell Adhesion Molecules (EpCAM) HepG2, increased levels cytokines Interleukin-4 (IL-4), Granulocyte-macrophage colony-stimulating factor (GM-CSF), Vascular Endothelial Growth Factor (VEGF), decreased albumin secretion. Furthermore, exert distinct microvascular with causing complete regression Conclusion This tumor-on-a-chip enables human-relevant, real-time assessment response, paving way development innovative therapies

Language: Английский

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Recent advancements and challenges in 3D bioprinting for cancer applications

Bioprinting, Journal Year: 2024, Volume and Issue: 43, P. e00357 - e00357

Published: Sept. 13, 2024

Language: Английский

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Organoid models of breast cancer in precision medicine and translational research

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 52(1)

Published: Nov. 21, 2024

Language: Английский

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