Site‐Selective Biofunctionalization of 3D Microstructures Via Direct Ink Writing DOI Creative Commons
George Mathew, Enrico Domenico Lemma,

Dalila Fontana

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract Two‐photon lithography has revolutionized multi‐photon 3D laser printing, enabling precise fabrication of micro‐ and nanoscale structures. Despite many advancements, challenges still persist, particularly in biofunctionalization microstructures. This study introduces a novel approach combining two‐photon with scanning probe for post‐functionalization microstructures overcoming limitations achieving spatially controlled biomolecule distribution. The method utilizes diverse range inks, including phospholipids, two different proteins, introducing high spatial resolution distinct functionalization on separate areas the same microstructure. surfaces are treated using bovine serum albumin and/or 3‐(Glycidyloxypropyl)trimethoxysilane (GPTMS) to enhance ink retention. further demonstrates strategies create binding sites cells by integrating biomolecules, showcasing potential customized cell microenvironments. Specific adhesion onto functionalized microscaffolds is demonstrated, which paves way applications tissue engineering, biointerfacing electronic devices biomimetic modeling.

Language: Английский

Printable Photonic Materials and Devices for Smart Healthcare DOI Open Access
Xinyi Zhang,

Kemeng Zhou,

Zhibin Zhao

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Photonic materials possess tunable optical properties and have been widely utilized for healthcare applications. These enable the detection of physical physiological bio-signals via modulated output characteristics, such as wavelength shifts, fluorescence emission, light scattering. When further synthesized into functional photonic inks, multimodal devices epidermal, minimally invasive, implantable bio-sensing can be constructed in facile printable manners. This review first introduces different geometries their unique properties. To feasible fabrication multi-functional biosensing versatile platforms, synthesis inks as-printed are then illustrated. Subsequently, advances breakthroughs to construct integrated systems wearable applications displayed, especially sensing facilitate personalized remote healthcare. Finally, challenges achieving mechanical stability, eliminated degradation, enhanced biocompatibility dynamic biological environments, scalable production discussed, along with prospects toward reliable intelligent

Language: Английский

Citations

0
Towards bi-material 3D printed soft microrobots using two-photon polymerisation DOI

Mehdi Salah,

Yannis Bordes,

Cédric Clévy

et al.

Journal of Micro-Bio Robotics, Journal Year: 2025, Volume and Issue: 21(1)

Published: March 13, 2025

Language: Английский

Citations

0
Dynamic Multi‐Focus 3D Laser Nanoprinting Based on Two‐Step Absorption and Computational Holography DOI Creative Commons
Pascal Rietz, Paul Somers, Sebastian Kalt

et al.

Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Two‐step‐absorption 3D laser nanoprinting allows for using compact and low‐cost continuous‐wave (cw) lasers. Therefore, it is an attractive alternative to established state‐of‐the‐art multi‐photon‐absorption nanoprinting. Additionally, low single‐focus polymerization‐threshold powers pave the way multi‐focus parallelization approaches while still cw sources. Parallelization further encouraged by fact that scanning velocities are inherently limited intermediate‐state lifetime of photoresist system used. Herein, a setup combines two‐step‐absorption with dynamic approach based on binary holography digital micromirror device (DMD) presented. The generated holographic patterns allow combined beam‐splitting beam‐steering as well control each focus’ individual intensity. one obtains versatile printing approach, enabling change number foci pattern, structure be printed. With this setup, microstructures up 31 in parallel at print rate achieved. capabilities limits chosen compared rate‐equation calculations.

Language: Английский

Citations

0
In Situ Control of Reactive Mesogens Alignment During 3D Printing by Two‐Photon Lithography DOI Creative Commons
Tiziana Ritacco, A. Mazzulla, M. Giocondo

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract Photopolymerizable liquid crystals, also known as reactive mesogens, are leading candidates for additive manufacturing of smart microdevices via two‐photon lithography (TPL). While substantial advancements made toward innovative applications, precise control molecular alignment during fabrication, essential tailoring complex optical and mechanical responses, remains a significant challenge. Current solutions require elaborate multi‐step procedures or customized setups to achieve 2D 3D patterns. Herein, the deterministic effect TPL on orientation mesogenic moieties is reported, under optimized printing conditions. Specifically, single‐step simple method developed aligning nematic director in situ, with sub‐diffraction‐limited resolution, printing. Based conventional workflow, “director‐tuning mode” (DiTuM) relies anisotropic photopolymerization reaction occurring along print path at low laser scan speeds (≈0.1mm s −1 ). A TPL‐induced “easy axis” arises moieties, programmable direction strength, competes initial create potentially convolute fields. The holds considerable promise 3D/4D printing, enabling advanced functionalities, offers robust platform anti‐counterfeiting leveraging unique signatures generated by microstructures.

Language: Английский

Citations

0
Architected Microalgae‐Based Matter via 3D Printing: Properties, Printing Techniques, and Applications DOI Creative Commons

Yiwei Zou,

J.H. Yi, Yunlu Dai

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

3D printing is a promising technology that enables the creation of intricate structures with tailorable properties, successfully transforming various fields, particularly in medical science, healthcare, and biomaterial technologies. Recent studies have recognized microalgae as sustainable, renewable, cost‐effective bioresources can be utilized bioinks for creating constructs intriguing functionalities, such oxygen‐generating scaffolds tissue engineering, engineered living materials, bioremediation. This review discusses properties applications microalgae, presents an overview current technology, provides comprehensive recent advancements 3D‐printed microalgae‐based diverse applications. Finally, challenges must overcome to ensure widespread applicability these materials are discussed. expected inspire future exploration innate compositions developing transformative potential biomedical biotechnological sectors.

Language: Английский

Citations

0
Thermal colour map of 4D smart microtags made by Two-Photon Lithography in cholesteric reactive mesogens DOI
Tiziana Ritacco, A. Mazzulla, Romeo Beccherelli

et al.

Liquid Crystals, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 9

Published: March 25, 2024

The helicoidal architecture of cholesteric reactive mesogens allows for smart 4D responsive microdevices that reflect selectively the wavelength and polarisation incident light. Two-photon lithography is an effective microprinting technology which also enables fine tuning Bragg reflection, as a function energy delivered during manufacturing polymerisation degree. When CRMs objects undergo temperature variation, thermal expansion causes elongation helix pitch consequent red-shift photonic band gap. A characterisation optical response microcylinders presented, showing tunable from 2 to 37 nm in 30–150°C range. enhanced tall structures, with high This exploited add security label QR code.

Language: Английский

Citations

2
Multimaterial 3D Laser Printing of Cell‐Adhesive and Cell‐Repellent Hydrogels DOI Creative Commons
Niklas Schwegler,

Tanisha Gebert,

Maria Villiou

et al.

Small, Journal Year: 2024, Volume and Issue: 20(33)

Published: May 6, 2024

Abstract Here, a straightforward method is reported for manufacturing 3D microstructured cell‐adhesive and cell‐repellent multimaterials using two‐photon laser printing. Compared to existing strategies, this approach offers bottom‐up molecular control, high customizability, rapid precise fabrication. The printable polyethylene glycol (PEG) based material includes an Arg‐Gly‐Asp (RGD) containing peptide synthesized through solid‐phase synthesis, allowing control of the design. Remarkably, minimal amounts RGD (< 0.1 wt%) suffice imparting cell‐adhesiveness, while maintaining identical mechanical properties in printed microstructures those cell‐repellent, PEG‐based material. Fluorescent labeling facilitates visualization its presence areas. To demonstrate broad applicability system, fabrication 2.5D structures shown, fostering adhesion fibroblast cells within these architectures. Thus, allows printing high‐resolution, true suitable diverse applications, including cellular studies complex environments.

Language: Английский

Citations

2
Printing Green: Microalgae‐based Materials for 3D Printing with Light DOI
Clara Vazquez‐Martel, Lilliana Flórido Martins, Elisa Genthner

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)

Published: June 14, 2024

Microalgae have emerged as sustainable feedstocks due to their ability fix CO

Language: Английский

Citations

2
Multimaterial 3D Laser Printing of Cell-Adhesive and Cell-Repellent Hydrogels DOI Creative Commons
Niklas Schwegler,

Tanisha Gebert,

Maria Villiou

et al.

Published: Jan. 17, 2024

This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability rapid precise fabrication. The printable PEG-based material includes an RGD-containing peptide synthesized through solid-phase synthesis, allowing control of the design. Remarkably, minimal amounts RGD (< 0.1 wt%) suffice imparting cell-adhesiveness, while maintaining identical mechanical properties in printed microstructures those cell-repellent, material. Fluorescent labeling facilitates visualization its presence areas. To demonstrate broad applicability our system, we showcase fabrication 2.5D structures, fostering adhesion fibroblast cells within these architectures. Thus, allows printing high-resolution, true structures suitable diverse applications, including cellular studies complex environments.

Language: Английский

Citations

1
Laser‐Inscribable, Moisture‐Responsive Cellulosic Supramolecular Structures with Tunable Broadband Coloration DOI

Shifu Lu,

Boyan Du,

Salman Ali Khan

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(46)

Published: July 4, 2024

Abstract Adaptive photonic films in response to external stimuli have broad applications optical communications, sensing, and anticounterfeiting. Yet, it remains challenging develop structures with tailorable fine patterns that display broadband color changing under ambient conditions. Here a hydrogen‐bonded supramolecular cholesteric liquid crystalline polymer (CLCP) is mediated by binary solvent consisting of citric acid (CA) water presented. The incorporation CA not only improves the long‐range order CLCPs through evaporation‐induced self‐assembly but also capable tuning their helical pitch across entire visible spectrum. extent hydration‐induced expansion can be further manipulated thermal crosslinking, enabling unique patterning strategy based on mask‐free, programmable laser inscription. High‐precision are created onto CLCPs, which reveal themselves upon hydration high visual contrast. study offers feasible up‐scaling route toward tailoring environmentally adaptive tunable coloration highly patterns.

Language: Английский

Citations

1