Low‐Crosslinked Hydrophilic Decellularized Adipose Matrix Hydrogels Achieve Efficient and Functional Xenotransplant Fat Regeneration by Optimizing Stem Cell Niche DOI

Xiaomu Ma,

Yue Qiang,

Su Fu

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Xenogeneic decellularized adipose matrix(DAM) scaffolds are significant in soft tissue regeneration by addressing donor site morbidity and limited availability. However, natural DAM xenotransplantation remains unachieved. Previous efforts have relied on chemical modifications to reduce foreign body reactions, with inherent drawbacks outcomes. This study proposes a hydrogel scaffold that achieves efficient functional xenotransplant fat regeneration. Primarily, hydrophilic DAM(H‐DAM) is confirmed lower antigen content, but its regenerative potential constrained encapsulation impedes seed cell infiltration. Thus, H‐DAM prepared enhance the speed extent of It observed topological structure impacts infiltration, immune inflammatory response, Low‐crosslinked hydrogels emerged as optimal for adipogenic differentiation due loose networks degradation rates synchronizing Furthermore, wall‐like rather than filamentous provides superior niche stem engraftment activity. Finally, integrated multi‐omics data analysis demonstrated regenerated functionally mimics native tissue. These findings indicate xenogeneic gel support both structural reconstruction tissue, offering novel insights promising approach clinical translation DAM‐based therapies.

Language: Английский

Static Topographical Cue Combined with Dynamic Fluid Stimulation Enhances the Macrophage Extracellular Vesicle Yield and Therapeutic Potential for Bone Defects DOI

Huayi Huang,

Lingfei Xiao,

Lucheng Fang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Extracellular vesicles (EVs) hold promise for tissue regeneration, but their low yield and limited therapeutic efficacy hinder clinical translation. Bioreactors provide a larger culture surface area stable environment large-scale EV production, yet ability to enhance is limited. Physical stimulation, by inducing cell differentiation modulating cargo composition, offers more efficient, cost-effective, reproducible approach compared the loading of EVs biochemical priming parental cells. Herein, effects 3D-printed perfusion bioreactor with topographical cue on macrophage bioactivity were assessed. The results indicate that increased 12.5-fold enhanced in promoting osteogenic angiogenesis via upregulated miR-210-3p. Mechanistically, fluid shear stress activates Piezo1, triggering Ca2+ influx Yes-associated protein (YAP) nuclear translocation, secretion enhancing M2 polarization conjunction morphological changes guided aligned topography. Moreover, porous electrospun membrane-hydrogel composite scaffold loaded bioreactor-derived exhibited outstanding rat cranial defect model. This study presents scalable, platform production EVs, potentially overcoming key challenges translating EV-based therapies clinic.

Language: Английский

Citations

1
Optimally Aligned Nerve Scaffolds with Sustained Astaxanthin Release Enhance the Inflammatory Microenvironment Via Mitophagy Activation DOI
Xue-Han Jin,

Shengfu Liu,

Jiaqi Fang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
GelMA/HA-NB hydrogel encapsulating adipose-derived chondrogenic exosomes enhances enthesis regeneration in chronic rotator cuff tears DOI
Yan Xu, Xin Shi,

Haofeng Lin

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142800 - 142800

Published: April 1, 2025

Language: Английский

Citations

0
Low‐Crosslinked Hydrophilic Decellularized Adipose Matrix Hydrogels Achieve Efficient and Functional Xenotransplant Fat Regeneration by Optimizing Stem Cell Niche DOI

Xiaomu Ma,

Yue Qiang,

Su Fu

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Xenogeneic decellularized adipose matrix(DAM) scaffolds are significant in soft tissue regeneration by addressing donor site morbidity and limited availability. However, natural DAM xenotransplantation remains unachieved. Previous efforts have relied on chemical modifications to reduce foreign body reactions, with inherent drawbacks outcomes. This study proposes a hydrogel scaffold that achieves efficient functional xenotransplant fat regeneration. Primarily, hydrophilic DAM(H‐DAM) is confirmed lower antigen content, but its regenerative potential constrained encapsulation impedes seed cell infiltration. Thus, H‐DAM prepared enhance the speed extent of It observed topological structure impacts infiltration, immune inflammatory response, Low‐crosslinked hydrogels emerged as optimal for adipogenic differentiation due loose networks degradation rates synchronizing Furthermore, wall‐like rather than filamentous provides superior niche stem engraftment activity. Finally, integrated multi‐omics data analysis demonstrated regenerated functionally mimics native tissue. These findings indicate xenogeneic gel support both structural reconstruction tissue, offering novel insights promising approach clinical translation DAM‐based therapies.

Language: Английский

Citations

0