Electric‐Field‐Driven Printed 3D Highly Ordered Microstructure with Cell Feature Size Promotes the Maturation of Engineered Cardiac Tissues

Advanced Science, Journal Year: 2023, Volume and Issue: 10(11)

Published: Feb. 13, 2023

Engineered cardiac tissues (ECTs) derived from human induced pluripotent stem cells (hiPSCs) are viable alternatives for repair, patient-specific disease modeling, and drug discovery. However, the immature state of ECTs limits their clinical utility. The microenvironment fabricated using 3D scaffolds can affect cell fate, is crucial maturation ECTs. Herein, authors demonstrate an electric-field-driven (EFD) printed highly ordered microstructure with feature size to promote simulation experimental results that EFD jet microscale printing overcomes repulsion without any prior requirements both conductive insulating substrates. Furthermore, microstructures a fiber diameter 10-20 µm spacing 60-80 have been by maintaining vertical jet, achieving largest ratio diameter/spacing 0.29. hiPSCs-derived cardiomyocytes formed sarcomere growth along developed synchronous functional inside 3D-printed scaffold matured calcium handling compared 2D coverslip. Therefore, process facilitates fabrication providing suitable ECTs, thereby showing great potential tissue engineering.

Language: Английский

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Formulation and Evaluation of PVA/Gelatin/Carrageenan Inks for 3D Printing and Development of Tissue‐Engineered Heart Valves

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(7)

Published: Oct. 10, 2023

Abstract Congenital and acquired valvular heart diseases (VHDs) are significant causes of mortality worldwide. With valve replacement being the primary solution for VHD, current options display shortcomings, including calcification, thrombogenicity, hemodynamic alteration, leading to repetitive surgeries. Tissue engineering, however, has shown great potential fabricating valves (HVs) with fewer complications. Here, a series inks developed, combining poly(vinyl alcohol), gelatin, carrageenan 3D printing tissue‐engineered (TEHVs). The inks/hydrogels investigated characterize their physico‐chemical, morphological, mechanical, rheological characteristics. In vitro in vivo biocompatibility, immune response, hemolysis, thrombogenicity also evaluated. Moreover, hydrodynamics TEHVs under physiological conditions reported. Inks demonstrate mechanical characteristics comparable native leaflets. Subcutaneous implantation reveals that hydrogels do not induce chronic inflammation can undergo remodeling. hemocompatibility assessments show minimal hemolysis low thrombogenicity. Different sizes types HVs successfully printed high fidelity air. hydrodynamic assessment confirms withstand aortic conditions. Altogether, 3D‐printed be promising alternative solve problems associated options.

Language: Английский

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Scaffolding fundamentals and recent advances in sustainable scaffolding techniques for cultured meat development

Food Research International, Journal Year: 2024, Volume and Issue: 189, P. 114549 - 114549

Published: May 26, 2024

In cultured meat (CM) production, Scaffolding plays an important role by aiding cell adhesion, growth, differentiation, and alignment. The existence of fibrous microstructure in connective muscle tissues has attracted considerable interest the realm tissue engineering triggered researchers to implement scaffolding techniques. A wide array research efforts is ongoing technologies for achieving real structure on principality biomedical replace serum free CM production. Scaffolds made animal-derived biomaterials are found efficient replicating extracellular matrix (ECM), thus focus should be paid utilize animal byproducts this purpose. Proper identification utilization plant-derived biomaterial could helpful add diversified options addition derived sources reduce cost production through scaffolds. Furthermore, techniques like electrospinning, modified electrospinning 3D bioprinting focused create porous scaffolds mimic ECM form meat-like structures. This review discusses recent advances cutting edge edible related structured

Language: Английский

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Harnessing Native Blueprints for Designing Bioinks to Bioprint Functional Cardiac Tissue

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 111882 - 111882

Published: Jan. 23, 2025

Cardiac tissue lacks regenerative capacity, making heart transplantation the primary treatment for end-stage failure. Engineered cardiac tissues developed through three-dimensional bioprinting (3DBP) offer a promising alternative. However, reproducing native structure, cellular diversity, and functionality of requires advanced bioinks. Major obstacles in CTE (cardiac engineering) include accurately characterizing bioink properties, replicating microenvironment, achieving precise spatial organization. Optimizing properties to closely mimic extracellular matrix (ECM) is essential, as deviations may result pathological effects. This review encompasses rheological electromechanical bioinks function microenvironment design functional constructs. Furthermore, it focuses on improving characteristics, printability, bioinks, offering valuable perspectives developing new especially designed CTE.

Language: Английский

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State-of-the-Art Nanoclay Reinforcement in Green Polymeric Nanocomposite: From Design to New Opportunities

Minerals, Journal Year: 2022, Volume and Issue: 12(12), P. 1495 - 1495

Published: Nov. 23, 2022

Nanoclays are layered aluminosilicate nanostructures. Depending upon the chemical composition and microscopic structure, various nanoclay types have been discovered such as montmorillonite, bentonite, kaolinite, halloysite nanoclay, etc. organically modified to develop compatibility with polymers. Polymer/nanoclay nanocomposites prompted significant breakthroughs in field of nanocomposite technology. Green form an important class nanomaterials using naturally derived degradable materials matrix/nanofiller. This review essentially deliberates fundamentals effect reinforcements green polymer matrices. Naturally polymers cellulose, starch, natural rubber, poly(lactic acid), employed these nanocomposites. polymer/nanoclay fabricated feasible fabrication approaches solution route, melt processing, situ polymerization, others. The significance structure-property relationships nanomaterials, essential attain desired features, has presented. light weight, inexpensiveness, ecofriendly, a low cost, enhanced indispensable physical properties. Consequently, found applications towards sustainability uses, packaging, membranes, biomedical (tissue engineering, drug delivery, wound healing) sectors. However, thorough research efforts desirable extend utility future technological

Language: Английский

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In situ 3D bioprinting: A promising technique in advanced biofabrication strategies

Bioprinting, Journal Year: 2023, Volume and Issue: 31, P. e00260 - e00260

Published: Feb. 2, 2023

Language: Английский

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3D Printing Approaches to Engineer Cardiac Tissue

Current Cardiology Reports, Journal Year: 2023, Volume and Issue: 25(6), P. 505 - 514

Published: May 2, 2023

Bioengineering of functional cardiac tissue composed primary cardiomyocytes has great potential for myocardial regeneration and in vitro modeling. 3D bioprinting was developed to create hydrogels that can mimic the structural, physiological, features native myocardium. Through a detailed review printing technologies bioink materials used creation heart tissue, this article discusses engineered tissues biomedical applications.

Language: Английский

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Recent Advances in Hydrogel-Based 3D Bioprinting and Its Potential Application in the Treatment of Congenital Heart Disease

Biomolecules, Journal Year: 2024, Volume and Issue: 14(7), P. 861 - 861

Published: July 18, 2024

Congenital heart disease (CHD) is the most common birth defect, requiring invasive surgery often before a child’s first birthday. Current materials used during CHD lack ability to grow, remodel, and regenerate. To solve those limitations, 3D bioprinting an emerging tool with capability create tailored constructs based on patients’ own imaging data grow remodel once implanted in children CHD. It has potential integrate multiple bioinks several cell types biomolecules within 3D-bioprinted that exhibit good structural fidelity, stability, mechanical integrity. This review gives overview of recent advancements technologies use treatment Moreover, selection appropriate biomaterials their chemical, physical, biological properties are further manipulated suit application also discussed. An introduction bioink formulations composed various emphasis briefly overviewed. Vasculogenesis angiogenesis prefabricated structures novel 4D printing technology summarized. Finally, we discuss restrictions our perspective future directions

Language: Английский

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Trends and Technological Challenges of 3D Bioprinting in Cultured Meat: Technological Prospection

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(22), P. 12158 - 12158

Published: Nov. 9, 2023

Cultured meat presents a possible alternative to conventional products and may be used address growing food demands attributable global population growth. Thus, comprehensive technological prospection of the scientific literature related cultured produced by 3D bioprinting is great interest researchers. The purpose this article review analyze published studies biofabrication using techniques. number publications in recent years highlights that has gained traction community. Furthermore, private companies startups have contributed advancements for consumption, illustrating as substitute already becoming reality. However, like any advance, faces challenges involving regulation, acceptance, selection ideal biomaterials cell lines, replacement fetal bovine serum (FBS), attaining texture nutritional value similar those meat.

Language: Английский

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Fabrication and characterization of a 3D scaffold based on elastomeric poly-glycerol Sebacate polymer for heart valve applications

Journal of Manufacturing Processes, Journal Year: 2023, Volume and Issue: 102, P. 350 - 364

Published: July 27, 2023

Language: Английский

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