Tutorial on impedance and dielectric spectroscopy for single-cell characterisation on microfluidic platforms: theory, practice, and recent advances

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Integration of low-frequency electrical impedance and broadband electromagnetic sensing with microfluidic devices enables high-throughput analysis cell size, membrane properties, intracellular characteristics.

Language: Английский

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Dielectrophoretic enrichment of live chemo-resistant circulating-like pancreatic cancer cells from media of drug-treated adherent cultures of solid tumors

Lab on a Chip, Journal Year: 2023, Volume and Issue: 24(3), P. 561 - 571

Published: Dec. 19, 2023

Due to low numbers of circulating tumor cells (CTCs) in liquid biopsies, there is much interest enrichment alternative circulating-like mesenchymal cancer cell subpopulations from

Language: Английский

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Label‐Free Microscale Technologies for Isolation of Heterogeneous Circulating Tumor Cells

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

The dissemination of primary solid tumor cells to distant organs, termed metastasis, is a major cause cancer‐related deaths. Circulating (CTCs), which can exist as individual or multicellular clusters, travel through the bloodstream. Their isolation from liquid biopsy samples increasingly recognized valuable tool for diagnosis, prognosis, and treatment guidance cancer patients. Current methods typically rely on biomarkers like epithelial cell adhesion molecule (EpCAM) utilize technologies such magnetic beads microfluidic chips. However, these face limitations due heterogeneity. Furthermore, that transfer into CTCs often undergo epithelial‐to‐mesenchymal transition, gaining invasive characteristics while losing markers. As result, are difficult detect using EpCAM‐based methods. Label‐free microscale tackle biomarker‐based by leveraging distinctive physical properties CTCs, their size, electrical charge, viscoelasticity, deformability contrast them normal blood cells. This review evaluates label‐free methods, including deterministic lateral displacement, microfiltration, acoustophoresis, dielectrophoresis, whether they offer deeper insight heterogeneity dynamics progression treatment. Additionally, it highlights automated platforms high‐throughput CTC analysis.

Language: Английский

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Isolation Techniques of Micro/Nano‐Scaled Species for Biomedical Applications

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Abstract Isolation of micro/nano‐scaled bioparticles, such as circulating tumor cells (CTCs), exosomes, bacteria, white blood (WBCs), platelets, and viruses, from the sample is essential for cancer diagnosis treatment, preventing bacterial infections, monitoring human health. Numerous separation techniques, including magnetophoresis, dielectrophoresis, acoustophoresis, optophoresis, fluorescence‐activated sorting (FAS) have been developed to isolate target bioparticles complex samples accurately. However, these active methods usually rely on sophisticated instruments which are expensive bulky. Passive platforms with high throughput, low cost, small volume gradually become alternative methods. Alongside this context, review paper no longer confined one specific category isolation advanced systems that in recent years comprehensively introduced. Characteristics limitations each technology discussed according critical performance parameters purity, recovery rate, resolution, size, convenience. Specific biomedical applications techniques summarized provide practical implications disease diagnosis, mechanism research. This also addresses current challenges, potential solutions, prospects field, laying foundation further optimization, innovation, cross‐integration future.

Language: Английский

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Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Micromachines, Journal Year: 2023, Volume and Issue: 14(11), P. 2117 - 2117

Published: Nov. 18, 2023

Blood is a complex sample comprised mostly of plasma, red blood cells (RBCs), and other whose concentrations correlate to physiological or pathological health conditions. There are also many blood-circulating biomarkers, such as circulating tumor (CTCs) various pathogens, that can be used measurands diagnose certain diseases. Microfluidic devices attractive analytical tools for separating components in point-of-care (POC) applications. These platforms have the potential advantage of, among features, being compact portable. features eventually exploited clinics rapid tests performed households low-income scenarios. systems added benefit only needing small volumes drawn from patients (from nanoliters milliliters) while integrating (within devices) steps required before detecting analytes. Hence, these will reduce associated costs purifying interest (e.g., specific groups biomarkers) studying quantifying collected fractions. The microfluidic separation field has grown since 2000s, important advances been reported last few years. Nonetheless, real POC still elusive. A widespread consensus on what key figures merit should assess quality yield not achieved. Knowing parameters separations help achieve establish clear road map promote further commercialization attain This review provides an overview techniques currently separate higher throughput (number particles per minute). We present summary critical considered when designing explicitly presenting device’s capabilities. Ultimately, reporting relevant this growing community pave toward systems.

Language: Английский

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Performance optimization of a DLD microfluidic device for separating deformable CTCs

Electrophoresis, Journal Year: 2024, Volume and Issue: 45(19-20), P. 1775 - 1784

Published: Aug. 14, 2024

Deterministic lateral displacement (DLD) microfluidic devices work based on the streamlines created by an array of micro-posts. The configuration pillars alters isolation efficiency these devices. present paper optimizes performance a DLD device for isolating deformable circulating tumor cells. input variables include cell diameter (d), Young's modulus (

Language: Английский

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Rapid High-Throughput Isolation and purification of Chicken Myoblasts Based on Deterministic Lateral Displacement Microfluidic Chips

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

Abstract Myoblasts are defined as stem cells containing skeletal muscle cell precursors. However, there some challenges associated with the purification of myoblast samples, including long culture times and ease bacterial contamination. In this study, we propose a microfluidic enrichment platform based on principle deterministic lateral displacement (DLD). To achieve this, designed DLD chip three outlets tested it 11-day-old Wenchang chicken pectoral tissue. A suspension was prepared using collagenase method, pretreated, then passed into for purification. number myoblasts diameter increased slowly before E9, myofibers decreased rapidly after E9. The period when fibers is highest E12, increases again lowest point also E12. After decreased. At clustered fused, proliferation greatly reduced. E12 both intact most vigorous period, so best time to determine isolation We attained recovery rate 80%, target outlet collection purity 99%, throughput 50 μ m/min. This study provides novel effective method myoblasts.

Language: Английский

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A microfluidic impedance cytometry device for robust identification of H. pluvialis

Analytical Methods, Journal Year: 2024, Volume and Issue: 16(33), P. 5684 - 5691

Published: Jan. 1, 2024

H. pluvialis contains rich oleic acid and astaxanthin, which have important applications in the fields of biodiesel biomedicine. Detection live is prerequisite to obtaining astaxanthin. For this purpose, we successfully developed a reliable microfluidic impedance cytometry for identification pluvialis. Firstly, established simulation model detecting based on their morphology studied effect medium conductivity at different frequencies. From simulations, determined that optimal solution detection was 1500 μS cm-1 frequency responses Secondly, fabricated microchannels stainless-steel electrodes assembled them into cytometry. The dependence dead explored under frequencies, were distinguished 1 MHz. MHz ranges from 33.73 52.23 Ω, while ones 13.05 19.59 Ω. Based these findings, accomplished counting sample solutions. Furthermore, mixed samples containing Euglena This approach possesses promising capacity serve as robust tool target microalgae, addressing challenge

Language: Английский

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Neural Network‐Enabled Multiparametric Impedance Signal Templating for High throughput Single‐Cell Deformability Cytometry Under Viscoelastic Extensional Flows

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Abstract Cellular biophysical metrics exhibit systematic alterations during processes, such as metastasis and immune cell activation, which can be used to identify separate live subpopulations for targeting drug screening. Image‐based cytometry under extensional flows accurately quantify deformability based on shape but needs extensive image reconstruction, limits its inline utilization activate sorting. Impedance measure these electric field screening, while frequency response offers functional information viability interior structure, are difficult discern by imaging. Furthermore, 1‐D temporal impedance signal trains characteristic shapes that rapidly templated in near real‐time extract single‐cell We present a multilayer perceptron neural network templating approach utilizes raw signals from cells flow, alongside training with corresponding derive net electrical anisotropy over wide ranges minimal errors size distributions. Deformability physiology applied conjunction the same multiparametric classification of pancreatic cancer versus associated fibroblasts using support vector machine model.

Language: Английский

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Rapid high-throughput isolation and purification of chicken myoblasts based on deterministic lateral displacement microfluidic chips

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0301309 - e0301309

Published: Dec. 5, 2024

Myoblasts are defined as stem cells containing skeletal muscle cell precursors. However, there some challenges associated with the purification of myoblast samples, including long culture times and ease bacterial contamination. In this study, we propose a microfluidic enrichment platform based on principle deterministic lateral displacement (DLD). To achieve this, designed DLD chip three outlets tested it 11-day-old (E11) Wenchang chicken pectoral tissue. A suspension was prepared using collagenase method, pretreated, then passed into for purification. number myoblasts diameter increased slowly before E9, myofibers decreased rapidly after E9. The period when fibers most numerous is E12, begins to increase again reaching its lowest point also E12. After decreased. At clustered fused, proliferation greatly reduced. E12 both intact vigorous period, so best time determine isolation We attained recovery rate 80%, target outlet collection purity 99%, throughput 50 μ m/min. paper, innovate chips design according specific geometries functions tissue, optimize process myoblasts. This study provides novel effective method

Language: Английский

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