FLight Biofabrication Supports Maturation of Articular Cartilage with Anisotropic Properties

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(12)

Published: Oct. 23, 2023

Tissue engineering approaches that recapitulate cartilage biomechanical properties are emerging as promising methods to restore the function of injured or degenerated tissue. However, despite significant progress in this research area, generation engineered constructs akin native counterparts still represents an unmet challenge. In particular, inability accurately reproduce zonal architecture with different collagen fibril orientations is a limitation. The arrangement extracellular matrix (ECM) plays fundamental role determining mechanical and biological functions study, it shown novel light-based approach, Filamented Light (FLight) biofabrication, can be used generate highly porous, 3D cell-instructive anisotropic lead directional deposition. Using photoclick-based photoresin optimized for tissue engineering, significantly improved maturation cartilaginous tissues remarkable native-like demonstrated.

Language: Английский

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Bioprinting: Mechanical Stabilization and Reinforcement Strategies in Regenerative Medicine

Tissue Engineering Part A, Journal Year: 2024, Volume and Issue: 30(13-14), P. 387 - 408

Published: Jan. 11, 2024

Bioprinting describes the printing of biomaterials and cell-laden or cell-free hydrogels with various combinations embedded bioactive molecules. It encompasses precise patterning cells to create scaffolds for different biomedical needs. There are many requirements that bioprinting face, it is ultimately interplay between scaffold's structure, properties, processing, performance will lead its successful translation. Among essential properties must possess—adequate appropriate application-specific chemical, mechanical, biological performance—the mechanical behavior hydrogel-based bioprinted key their stable in vivo at site implantation. Hydrogels typically constitute main scaffold material medium biomolecules very soft, often lack sufficient stability, which reduces printability and, therefore, potential. The aim this review article highlight reinforcement strategies used approaches achieve enhanced stability bioinks printed scaffolds. Enabling robust materials processes creation truly complex remarkable structures could accelerate application smart, functional settings. a powerful tool fabrication 3D applications. has gained tremendous attention recent years, bioink library expanding include more combinations. From practical perspective, need be considered, such as structure's performances. these, constructs critical translation into clinic. explore stabilization structures.

Language: Английский

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Exploring the Impact of Volumetric Additive Manufacturing of Photo-crosslinkable Gelatin on Mesenchymal Stromal Cell Behavior and Differentiation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Abstract This study explores the application of photo-crosslinkable gelatin hydrogels (constituting GelSH and GelNB) for biofabrication using volumetric additive manufacturing (VAM). Three variants were prepared, yielding degree substitutions (DS) 39%, 54%, 63%, GelNB with a DS 60%, alongside GelNB-GelSH formulations at concentrations 5, 7.5, 10% (w/v). Physico-chemical analyses confirmed that these variations led to differences in mass swelling ratio mechanical properties. VAM enabled fabrication complex, high-fidelity 3D structures from optimized formulations, demonstrating its capacity encapsulate mesenchymal stromal cells (MSCs) within biomimetic matrix. Compared film casted constructs, VAM-printed scaffolds exhibited significantly higher ALP activity calcium deposition, confirming effective osteogenesis. Conversely, chondrogenic adipogenic differentiation more pronounced groups due their lower crosslinking density compressive elastic modulus, emphasizing role environment guiding cell differentiation. highlights potential produce complex functional hydrogel tissue engineering, lays groundwork future work on finetuning bioresin enhance across multiple lineages various engineering applications, including softer tissues. Figure investigates (GelSH (VAM) biofabrication. Different influenced produced encapsulating cells, enhancing osteogenesis compared film-cast constructs. The findings highlight VAM’s optimizing bioresins multi-lineage differentiation,

Language: Английский

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Biotechnological advances in 3D modeling of cancer initiation. Examples from pancreatic cancer research and beyond

Biofabrication, Journal Year: 2025, Volume and Issue: 17(2), P. 022008 - 022008

Published: Feb. 28, 2025

In recent years, biofabrication technologies have garnered significant attention within the scientific community for their potential to create advancedin vitrocancer models. While these been predominantly applied model advanced stages of cancer, there exists a pressing need develop pertinent, reproducible, and sensitive 3D models that mimic cancer initiation lesions native tissue microenvironment. Such hold profound relevance comprehending intricacies initiation, devise novel strategies early intervention, and/or conduct sophisticated toxicology assessments putative carcinogens. Here, we will explain pivotal factors must be faithfully recapitulated when constructing models, with specific focus on pancreatic lesions. By synthesizing current state research in this field, provide insights into advances breakthroughs. Additionally, delineate key technological biological challenges necessitate resolution future endeavors, thereby paving way more accurate insightfulin

Language: Английский

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Advances in vat photopolymerization: early-career researchers shine light on a path forward

RSC Applied Polymers, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Early-career researchers from around the world summarize recent developments and present visions for future of vat photopolymerization 3D printing.

Language: Английский

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Microfiber‐Templated Porogel Bioinks Enable Tubular Interfaces and Microvascularization Down to the Building Blocks for 3D Bioprinting

Small, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract Vascularization is key to the biofabrication of large‐scale tissues. Despite progress, there remain some outstanding challenges, such as limited vessel density, difficulty in fabricating microvasculatures, and inhomogeneity post‐seeding cells. Here, a new form bioink called microfiber‐templated porogel (µFTP) introduced engineer vasculatures down filament building blocks 3D bioprinted hydrogels. The cell‐laden sacrificial microfibers (diameter ranges from 50–150 µm) are embedded template tubular voids deliver endothelial cells for in‐situ endothelialization. inclusion softening hydrogel retains desirable rheological properties extrusion‐based bioprinting well inter‐contacted extruded filament. Such bioinks can be printed into well‐defined structure with tunable porosities up 55%. Compared conventional bulk counterpart, µFTP supports significant growth spread either matrix or fibers, free post‐cell seeding procedure. Furthermore, scaffolds based on seen significantly promote in‐growth blood vessels native tissues vivo. approach enables engineering bio‐interfaces within contributes endothelialization providing versatile tool construction customized vascularized tissue models.

Language: Английский

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The road ahead in materials and technologies for volumetric 3D printing

Nature Reviews Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Language: Английский

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Towards single-cell bioprinting: micropatterning tools for organ-on-chip development

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(6), P. 739 - 759

Published: Feb. 2, 2024

Organs-on-chips (OoCs) hold promise to engineer progressively more human-relevant in vitro models for pharmaceutical purposes. Recent developments have delivered increasingly sophisticated designs, yet OoCs still lack reproducing the inner tissue physiology required fully resemble native human body. This review emphasizes need include microarchitectural and microstructural features, discusses promising avenues incorporate well-defined microarchitectures down single-cell level. We highlight how their integration will significantly contribute advancement of field towards highly organized structural hierarchical tissues-on-chip. discuss combination state-of-the-art micropatterning technologies achieve resembling human-intrinsic complexity. It is anticipated that these innovations yield significant advances realization next generation OoC models.

Language: Английский

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Virtual Volumetric Additive Manufacturing (VirtualVAM)

Advanced Materials Technologies, Journal Year: 2023, Volume and Issue: 8(23)

Published: Oct. 6, 2023

Abstract Tomographic volumetric additive manufacturing (VAM) produces arbitrary 3D geometries by exposure of a rotating volume photopolymer resin to tomographically‐patterned illumination. This enables high speed, layer‐less printing parts from wide range photopolymers not amenable layer‐by‐layer processes. Since the entire geometry is produced at once over course few seconds minutes, molecular diffusion length scales become significant process. Understanding these reaction and processes imperative for advancing VAM usable technology. These are experimentally very difficult monitor measure. Herein, VirtualVAM ‐ simulation framework modeling tomographic process, developed validated. simulates reaction, diffusion, heat generation print with single‐voxel resolution. From experimentally‐determined input parameters set images projection, able generate large spatio‐temporal data any given print. Using VirtualVAM, number experimentally‐unattainable aspects process investigated such as conversion profiles, effect oxygen, stopping time determination. also optimization patterns further improve contrast between in‐part out‐of‐part delivered dose.

Language: Английский

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Embedding biomimetic vascular networks via coaxial sacrificial writing into functional tissue

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 30, 2024

Abstract Printing human tissue constructs replete with biomimetic vascular networks is of growing interest for and organ engineering. While it now possible to embed perfusable channels within acellular densely cellular matrices, they lack either the branching or multilayer architecture native vessels. Here, we report a generalizable method printing hierarchical soft living matrices. We vessels into granular hydrogel matrices via coaxial embedded (co-EMB3DP) as well bulk cardiac tissues sacrificial writing functional (co-SWIFT). Each relies on an extended core-shell printhead that promote facile interconnections between printed Though careful optimization multiple inks show can be coaxially printed, which possess smooth muscle cell-laden shell surrounds lumens. Upon seeding these confluent layer endothelial cells, exhibit good barrier function. As final demonstration, construct vascularized composed matrix spheroids derived from induced pluripotent stem cells. Importantly, co-SWIFT mature under perfusion, beat synchronously, cardio-effective drug response in vitro. This advance opens new avenues scalable biomanufacturing organ-specific testing, disease modeling, therapeutic use.

Language: Английский

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