Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Organs-on-chips (OoCs) can be directly fabricated by 3D bioprinting techniques, which enhance the structural and functional fidelity of organ models broaden applications OoCs.
Language: Английский
Critical Reviews in Biotechnology, Journal Year: 2022, Volume and Issue: 43(3), P. 465 - 483
Published: April 11, 2022
Microfluidics is revolutionizing the way research on cellular biology has been traditionally conducted. The ability to control cell physicochemical environment by adjusting flow conditions, while performing analysis at single-cell resolution and high-throughput, made microfluidics ideal choice replace traditional in vitro models. However, such a revolution only truly started with advent of polydimethylsiloxane (PDMS) as microfluidic structural material soft-lithography rapid manufacturing technology. Indeed, before "PDMS age," technologies were: costly, time-consuming and, more importantly, accessible specialized laboratories users. simplicity molding PDMS various shapes along its inherent properties (transparency, biocompatibility, gas permeability) spread applications innovative devices diverse important biological fields clinical studies. This review highlights how PDMS-based systems are innovating pre-clinical cells organs. These were able cultivate different lines, enhance sensitivity diagnostic effectiveness numerous cell-based assays maintaining consistent chemical gradients, utilizing detecting smallest number analytes being high-throughput. will also assist identifying pitfalls current facilitate breakthroughs advancements healthcare research.
Language: Английский
Citations
43
Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10
Published: June 15, 2023
The 3Rs principle of replacing, reducing and refining the use animals in science has been gaining widespread support international research community appears transnational legislation such as European Directive 2010/63/EU, a number national legislative frameworks like Switzerland UK, other rules guidance place countries around world. At same time, progress technical biomedical research, along with changing status many societies, challenges view sufficient effective approach to moral set by animal research. Given this growing awareness our responsibilities animals, aim paper is address question: Can 3Rs, policy instrument for still guide morally acceptable scientific purposes, if so, how? fact that increased availability alternatives models not correlated inversely decrease used led public political calls more radical action. However, focus on simple measure total numbers distracts from need nuanced understanding how can have genuine influence guiding testing. Hence, we three core dimensions contemporary research: (1) What
Language: Английский
Citations
40
European Respiratory Review, Journal Year: 2024, Volume and Issue: 33(171), P. 230127 - 230127
Published: Jan. 17, 2024
Lung fibrosis is a complex process, with unknown underlying mechanisms, involving various triggers, diseases and stimuli. Different cell types (epithelial cells, endothelial fibroblasts macrophages) interact dynamically through multiple signalling pathways, including biochemical/molecular mechanical signals, such as stiffness, affecting function differentiation. Idiopathic pulmonary (IPF) the most common fibrosing interstitial lung disease (fILD), characterised by notably high mortality. Unfortunately, effective treatments for advanced fILD, especially IPF non-IPF progressive phenotype ILD, are still lacking. The development of pharmacological therapies faces challenges due to limited knowledge pathogenesis absence pre-clinical models accurately representing features disease. To address these challenges, new model systems have been developed enhance translatability preclinical drug testing bridge gap human clinical trials. use two- three-dimensional in vitro cultures derived from healthy or diseased individuals allows better understanding mechanisms responsible fibrosis. Additionally, microfluidics systems, which replicate respiratory system's physiology ex vivo , offer promising opportunities therapies, IPF.
Language: Английский
Citations
13
Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 372, P. 751 - 777
Published: July 4, 2024
Despite significant advances, cancer remains a leading global cause of death. Current therapies often fail due to incomplete tumor removal and nonspecific targeting, spurring interest in alternative treatments. Hyperthermia, which uses elevated temperatures kill cells or boost their sensitivity radio/chemotherapy, has emerged as promising alternative. Recent advancements employ nanoparticles (NPs) heat mediators for selective cell destruction, minimizing damage healthy tissues. This approach, known NP hyperthermia, falls into two categories: photothermal (PTT) magnetothermal (MTT). PTT utilizes NPs that convert light heat, while MTT magnetic activated by alternating fields (AMF), both achieving localized damage. These methods offer advantages like precise minimal invasiveness, reduced systemic toxicity. However, the efficacy hyperthermia depends on many factors, particular, properties, microenvironment (TME), TME-NP interactions. Optimizing this treatment requires accurate monitoring strategies, such nanothermometry biologically relevant screening models can better mimic physiological features human body. review explores state-of-the-art NP-mediated discussing available nanomaterials, strengths weaknesses, characterization methods, future directions. Our particular focus lies preclinical techniques, providing an updated perspective relevance journey towards clinical trials.
Language: Английский
Citations
11
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Organs-on-chips (OoCs) can be directly fabricated by 3D bioprinting techniques, which enhance the structural and functional fidelity of organ models broaden applications OoCs.
Language: Английский
Citations
1