A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

National Science Review, Journal Year: 2022, Volume and Issue: 9(8)

Published: June 3, 2022

The outbreak of the COVID-19 pandemic was partially due to challenge identifying asymptomatic and presymptomatic carriers virus, thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On other hand, several concerning SARS-CoV-2 variants, including Omicron, are required identified as soon samples 'positive'. Unfortunately, traditional PCR test does not allow their specific identification. Herein, first time, we have developed MOPCS (Methodologies Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) 'gene scissors' clustered regularly interspaced short palindromic repeat (CRISPR) technique achieve both specificity when it comes measurement viral variants. is low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform analyze RNA, without need amplification, within 38 min from sample input results output, limit detection 15 fM. achieves highly sensitive analysis SARS-CoV-2, mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) BA.1 (a subtype Omicron). This also used some recently collected patient local China, by Centers Disease Control Prevention. innovative CRISPR-empowered will further contribute fast, accurate target nucleic acid sequences single-base mutations.

Language: Английский

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A Highly Sensitive CRISPR‐Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar‐Level Real‐Time Quantification

Advanced Science, Journal Year: 2022, Volume and Issue: 9(14)

Published: March 27, 2022

The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system has emerged as a promising technology for the detection of nucleic acids. Herein, development surface plasmon resonance (SPR) sensor that is functionalized with layer locally grown graphdiyne film, achieving excellent sensing performance when coupled catalytically deactivated CRISPR-associated protein 9 (dCas9), reported. dCas9 immobilized on and complexed specific single-guide RNA, enabling amplification-free target sequences within genomic DNA. sensor, termed CRISPR-SPR-Chip, used to successfully analyze recombinant plasmids only three-base mutations limit low 1.3 fM. Real-time monitoring CRISPR-SPR-Chip clinical samples patients Duchenne muscular dystrophy two exon deletions, which are detected without any pre-amplification step, yielding significantly positive results 5 min. ability this novel CRISPR-empowered SPR (CRISPR-eSPR) platform rapidly, precisely, sensitively, specifically detect gene sequence provides new on-chip optic approach analysis.

Language: Английский

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A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs

Nature Biomedical Engineering, Journal Year: 2023, Volume and Issue: 7(12), P. 1583 - 1601

Published: April 27, 2023

Language: Английский

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Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 52(1), P. 361 - 382

Published: Dec. 19, 2022

This review summarizes the recent advances and main strategies to improve sensitivity of amplification-free CRISPR/Cas-based detection techniques.

Language: Английский

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Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs

Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(10)

Published: May 1, 2024

Abstract Early lung cancer screening by computed tomography is hampered pulmonary nodules caused massive COVID‐19 infections, necessitating an ultrasensitive approach for the early diagnosis of cancers at single‐base level from circular tumor DNAs (ctDNAs). This study introduces that merges DNA origami and scissors technologies in a framework surface plasmon resonance (SPR) biosensors. By combining precision probes with inherent resolution scissors, this method systematically addresses limitations conventional SPR techniques, resulting enhanced detection accuracy. The synergistic interplay between enables biosensors to achieve unprecedented levels sensitivity, precision, practical utility. efficacy allows precise identification mutations, demonstrated here T790M mutation EGFR gene G12C KRAS non‐small cell patients. With technique, as well near zeptomolar‐level sensitivity achieved. As result, discovery holds significant potential advance field diagnostics.

Language: Английский

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Ultrasensitive and Specific Clustered Regularly Interspaced Short Palindromic Repeats Empowered a Plasmonic Fiber Tip System for Amplification-Free Monkeypox Virus Detection and Genotyping

ACS Nano, Journal Year: 2023, Volume and Issue: 17(13), P. 12903 - 12914

Published: June 29, 2023

The urgent necessity for highly sensitive diagnostic tools has been accentuated by the ongoing mpox (monkeypox) virus pandemic due to complexity in identifying asymptomatic and presymptomatic carriers. Traditional polymerase chain reaction-based tests, despite their effectiveness, are hampered limited specificity, expensive bulky equipment, labor-intensive operations, time-consuming procedures. In this study, we present a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-based platform with surface plasmon resonance-based fiber tip (CRISPR-SPR-FT) biosensor. compact CRISPR-SPR-FT biosensor, 125 μm diameter, offers high stability portability, enabling exceptional specificity diagnosis precise identification of samples fatal mutation site (L108F)

Language: Английский

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A Novel Plasmonic MIM Sensor Using Integrated 1 × 2 Demultiplexer for Individual Lab-on-Chip Detection of Human Blood Group and Diabetes Level in the Visible to Near-Infrared Region

IEEE Sensors Journal, Journal Year: 2024, Volume and Issue: 24(8), P. 12034 - 12041

Published: March 8, 2024

In this article, we present an innovative plasmonic lab-on-chip (LoC) sensor featuring three ports, utilizing a $1\times2$ demultiplexer to detect two separate samples without cross-contamination. The performance of the is numerically investigated using finite element method (FEM), yielding maximum sensitivity 865.9 nm per refractive index unit (RIU) and figure merit (FOM) 58.4 in 700–920 range. proposed metal–insulator–metal (MIM) structure, incorporating diamond-shaped silicon nanodot array with perimeter less than 100 nm, serves as sensing surface, detecting changes (RI) surrounding medium. Our suggested demonstrates extraordinary transmission coefficient (TC) around −18-dB, it surpasses classical optics predictions, its nanoscale light confinement enhances interaction analytes. LoC sensor, based on MIM achieves high accuracy precision measuring small RI changes, making portable suitable for on-site applications, settings limited resources. This streamlines medical processes by rapidly blood groups diabetes levels minimal sample volume, potentially improving patient care efficiency.

Language: Английский

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Nucleic acid-assisted CRISPR-Cas systems for advanced biosensing and bioimaging

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 159, P. 116931 - 116931

Published: Jan. 11, 2023

Language: Английский

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Emerging two-dimensional materials: Synthesis, physical properties, and application for catalysis in energy conversion and storage

The Innovation Materials, Journal Year: 2024, Volume and Issue: 2(1), P. 100060 - 100060

Published: Jan. 1, 2024

<p>Inorganic, organic, and hybrid two-dimensional (2D) materials are being developed for ever-expanding numbers of applications, though energy catalysis remain the main drivers their development. We present overviews bottom-up top-down synthetic strategies such examine manufacturing scalability issues. Mechanical, electrical, thermal properties modulation highlighted because they fundamental to above-mentioned drivers. The burgeoning importance heterostructures in materials, particularly electrode design function is stressed. Detailed attention given applications 2D electrocatalysis reactions: oxygen reduction, evolution, hydrogen carbon dioxide nitrogen reduction. Water splitting, reduction by photocatalysis also examined. A perspective expected advances expansion types with a focus on heterostructure development, presented conclusion.</p>

Language: Английский

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MBenes for Energy Conversion: Advances, Bottlenecks, and Prospects

Langmuir, Journal Year: 2024, Volume and Issue: 40(21), P. 10835 - 10846

Published: May 13, 2024

The advent of two-dimensional layered materials has bolstered the development catalytic endeavors for energy conversion and storage. MXenes (transition metal carbides/nitrides) have already consolidated their candidature in past decade due to enhanced compositional structural tunabilities through surface modifications. Perseverant research engineering MXene based led inception MBenes borides) as promising systems energy-driven operations. Physicochemical superiorities such escalated conductivity hydrophilicity, unique geometrical domains, higher stability modulus elasticity provide reaction-friendly milieu exploit these materials. Nevertheless, on is embryonic requires thorough realization scientific significance. Herein, we aim discuss advancements, challenges, outlooks with respect HER, CO2RR, NRR applications.

Language: Английский

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