Cascaded Ω-Shaped fiber optic-based LSPR coated with hybridized nanolayers for refractive index and temperature simultaneous measurement DOI
Mingyue Li,

Binbin Lu,

Kun Xu

et al.

Optics Letters, Journal Year: 2025, Volume and Issue: 50(6), P. 1803 - 1803

Published: Feb. 18, 2025

It is important to monitor refractive index (RI) and temperature (T) simultaneously for photo-thermal therapy (PTT) in the blood circulation system. However, fluctuation of T sways RI response owing high cross-sensitivity. To address issue, a fiber-optic-based localized surface plasmon resonance (LSPR) sensor with cascaded structure single-mode fiber-multimode fiber-single-mode fiber (SMF-MMF-SMF) presented synchronous measurement T. This detection principle founded on synergistic effects Mach-Zehnder interference (MZI) LSPR. The was fabricated inspire MZI, while LSPR excited by gold nanoparticles/polydopamine (AuNPs/PDA) deposited surface. MZI spectra were extracted reconstructed using spectrum transformation filtering techniques sort original spectra. achieved simultaneous sensitivity 3.58 (a.u.)/RIU -0.0011 (a.u.)/°C, respectively. Additionally, signals near wavelength absorption are used replace signal. Compared signal, signal exhibited over 30 times enhancement figure merit (FOM) value due its narrow full width at half maxima (FWHM). provided novel strategy T, making it promising alternative vivo therapy.

Language: Английский

Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs DOI
Zhi Chen,

Changle Meng,

Xueliang Wang

et al.

Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(10)

Published: May 1, 2024

Abstract Early lung cancer screening by computed tomography is hampered pulmonary nodules caused massive COVID‐19 infections, necessitating an ultrasensitive approach for the early diagnosis of cancers at single‐base level from circular tumor DNAs (ctDNAs). This study introduces that merges DNA origami and scissors technologies in a framework surface plasmon resonance (SPR) biosensors. By combining precision probes with inherent resolution scissors, this method systematically addresses limitations conventional SPR techniques, resulting enhanced detection accuracy. The synergistic interplay between enables biosensors to achieve unprecedented levels sensitivity, precision, practical utility. efficacy allows precise identification mutations, demonstrated here T790M mutation EGFR gene G12C KRAS non‐small cell patients. With technique, as well near zeptomolar‐level sensitivity achieved. As result, discovery holds significant potential advance field diagnostics.

Language: Английский

Citations

70
Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device DOI Creative Commons
Yunxiang Wang, Hong Chen, Kai Lin

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 16, 2024

Abstract The emerging monkeypox virus (MPXV) has raised global health concern, thereby highlighting the need for rapid, sensitive, and easy-to-use diagnostics. Here, we develop a single-step CRISPR-based diagnostic platform, termed SCOPE (Streamlined CRISPR On Pod Evaluation platform), field-deployable ultrasensitive detection of MPXV in resource-limited settings. viral nucleic acids are rapidly released from rash fluid swab, oral saliva, urine samples 2 min via streamlined lysis protocol, followed by 10-min recombinase polymerase amplification (RPA)-CRISPR/Cas13a reaction. A pod-shaped vest-pocket analysis device achieves whole process reaction execution, signal acquisition, result interpretation. can detect as low 0.5 copies/µL (2.5 copies/reaction) within 15 sample input to answer. We validate developed assay on 102 clinical male patients / volunteers, testing results 100% concordant with real-time PCR. single-molecular level sensitivity minutes simplified procedure performed miniaturized wireless device, which is expected spur substantial progress enable practice application diagnostics techniques point-of-care setting.

Language: Английский

Citations

35
Recent Advances in Two-Dimensional Nanomaterials for Healthcare Monitoring DOI

Arun Kumar Manoharan,

Mohamed Ismail Kamal Batcha,

Shanmugam Mahalingam

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 1706 - 1734

Published: April 2, 2024

The development of advanced technologies for the fabrication functional nanomaterials, nanostructures, and devices has facilitated biosensors analyses. Two-dimensional (2D) with unique hierarchical structures, a high surface area, ability to be functionalized target detection at surface, exhibit potential biosensing applications. electronic properties, mechanical flexibility, optical, electrochemical, physical properties 2D nanomaterials can easily modulated, enabling construction platforms various analytes targeted recognition, sensitivity, selectivity. This review provides an overview recent advances in nanostructures used biosensor wearable-sensor healthcare health-monitoring Finally, advantages 2D-nanomaterial-based several challenges their optimal operation have been discussed facilitate smart high-performance future.

Language: Английский

Citations

19
Potential Biomedical Applications of Terbium-Based Nanoparticles (TbNPs): A Review on Recent Advancement DOI
Sourav Mohanto, Aritra Biswas, Amol D. Gholap

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(5), P. 2703 - 2724

Published: April 22, 2024

The scientific world is increasingly focusing on rare earth metal oxide nanomaterials due to their consequential biological prospects, navigated by breakthroughs in biomedical applications. Terbium belongs elements (lanthanide series) and possesses remarkably strong luminescence at lower energy emission signal transduction properties, ushering wide applications for diagnostic measurements (i.e., bioimaging, biosensors, fluorescence imaging, etc.) the sectors. In addition, theranostic of terbium-based nanoparticles further permit targeted delivery drugs specific site disease. Furthermore, antimicrobial properties terbium induced via reactive oxygen species (ROS) cause oxidative damage cell membrane nuclei living organisms, ion release, surface charge interaction, thus creating or exhibiting excellent antioxidant characteristics. Moreover, recent tissue engineering, wound healing, anticancer activity, etc., angiogenesis, proliferation, promotion growth factors, biocompatibility, cytotoxicity mitigation, anti-inflammatory potentials, make this nanoparticle anticipate a future epoch nanomaterials. stand as game changer realm research, proffering array possibilities, from revolutionary imaging techniques advanced drug systems. Their unique including luminescence, magnetic characteristics, have redefined boundaries what can be achieved biomedicine. This review primarily delves into various mechanisms involved physicochemical article explains potential with in-depth significant individual literature. additionally stands first instance furnish "single-platted" comprehensive acquaintance shaping healthcare well limitations overcoming strategies that require exploration before being trialed clinical settings.

Language: Английский

Citations

18
CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection DOI

Zhiruo Yang,

Siying Mao,

Lu Wang

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(10), P. 3623 - 3642

Published: Oct. 11, 2023

Over the past few decades, pathogens have posed a threat to human security, and rapid identification of should be one ideal methods prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive specific approaches identify quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems Argonaute (Ago) belong Microbial Defense Systems (MDS). The guided, programmable, targeted activation nucleases by both them leading way new generation detection. We compare these two in terms similarities differences. In addition, we discuss future challenges prospects development biosensors, especially electrochemical biosensors. This review expected afford researchers entering this multidisciplinary field useful guidance provide inspiration more innovative biosensors

Language: Английский

Citations

26
Metal-based nanowires in electrical biosensing DOI

Shen-Jie Zhong,

Kang-Yu Chen,

Shaolei Wang

et al.

Rare Metals, Journal Year: 2024, Volume and Issue: 43(12), P. 6233 - 6254

Published: July 17, 2024

Language: Английский

Citations

13
CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases DOI

Yupiao Xia,

Ruotong Rao,

Mengqiu Xiong

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 8091 - 8108

Published: March 7, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious DiseasesYupiao XiaYupiao XiaState Key Laboratory Magnetic Resonance and Atomic Molecular Physics, in Biological Systems, National Center Wuhan, Wuhan Institute Physics Mathematics, Innovation Academy Precision Measurement Science Technology, Chinese Sciences − Optoelectronics, 430071, ChinaUniversity Sciences, Beijing 100049, ChinaMore by Yupiao XiaView Biography, Ruotong RaoRuotong RaoState RaoView Mengqiu XiongMengqiu XiongState ChinaDepartment Medicine, Nanjing First Hospital, Medical University, 210006, XiongView Bangshun He*Bangshun HeDepartment China*[email protected]More HeView Bingxin ZhengBingxin ZhengState ZhengView Yanwei JiaYanwei JiaState-Key Analog Mixed-Signal VLSI, Microelectronics, University Macau, Macau 999078, JiaView Biographyhttps://orcid.org/0000-0001-5795-7450, Ying Li*Ying LiState LiView Biographyhttps://orcid.org/0000-0001-8689-5578, Yunhuang Yang*Yunhuang YangState ChinaOptics Valley Laboratory, Hubei 430074, YangView BiographyCite this: Anal. Chem. 2024, 96, 20, 8091–8108Publication Date (Web):March 7, 2024Publication History Received27 September 2023Accepted23 February 2024Revised7 2024Published online7 March inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.3c04363https://doi.org/10.1021/acs.analchem.3c04363review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views1329Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluorescence,Genetics,Nucleic acids,SARS-CoV-2 Get e-Alerts

Language: Английский

Citations

12
State-of-the-art strategies of surface plasmon resonance biosensors in clinical analysis: A comprehensive review DOI
Jie Zhou, Yue Wang, Guo‐Jun Zhang

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 520, P. 216149 - 216149

Published: Aug. 17, 2024

Language: Английский

Citations

12
Ultra-Sensitive Detection of the SARS-CoV-2 Nucleocapsid Protein via a Clustered Regularly Interspaced Short Palindromic Repeat/Cas12a-Mediated Immunoassay DOI
Wen Yin,

Leyao Li,

Yang Yang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(6), P. 3150 - 3157

Published: May 8, 2024

Tracking trace protein analytes in precision diagnostics is an ongoing challenge. Here, we developed ultrasensitive detection method for the of SARS-CoV-2 nucleocapsid (N) by combining enzyme-linked immunosorbent assay (ELISA) with clustered regularly interspaced short palindromic repeat/CRISPR-associated (CRISPR/Cas) system. First, N bound capture antibody adsorbed on well plate was sequentially coupled primary antibody, biotinylated secondary and streptavidin (SA), followed biotin primer binding to SA. Subsequently, rolling circle amplification initiated generate ssDNA strands, which were targeted CRISPR/Cas12a cleave FAM-ssDNA-BHQ1 probe

Language: Английский

Citations

10
CRISPR/Cas12a antifouling nanocomposite electrochemical biosensors enable amplification-free detection of Monkeypox virus in complex biological fluids DOI

Jeong‐Chan Lee,

Seuk-Min Ryu,

Yongjin Lee

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(23), P. 11318 - 11326

Published: Jan. 1, 2024

The escalating global threat of infectious diseases, including monkeypox virus (MPXV), necessitates advancements in point-of-care diagnostics, moving beyond the constraints conventional methods tethered to centralized laboratories. Here, we introduce multiple CRISPR RNA (crRNA)-based biosensors that can directly detect MPXV within 35 minutes without pre-amplification, leveraging enhanced sensitivity and antifouling attributes BSA-based nanocomposite. Multiple crRNAs, strategically targeting diverse regions F3L gene MPXV, are designed combined amplify Cas12a activation its collateral cleavage reporter probes. Notably, our electrochemical sensors exhibit detection limit 669 fM amplification, which is approximately a 15-fold improvement compared fluorescence detection. This sensor also shows negligible changes peak current after exposure complex biological fluids, such as whole blood serum, maintaining at 682 fM. nearly identical conditions when only was present PBS. In summary, CRISPR-based be utilized high-performance diagnostic tool resource-limited settings, representing transformative leap forward testing. Beyond implications this technology extend various molecular establishing itself rapid, accurate, versatile platform for target analytes.

Language: Английский

Citations

9