Application and prospect of microfluidic devices for rapid assay of cell activities in the tumor microenvironment DOI

Linjing Zhu,

Xueling Cui, Lingling Jiang

et al.

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(3)

Published: May 1, 2024

The global impact of cancer on human health has raised significant concern. In this context, the tumor microenvironment (TME) plays a pivotal role in tumorigenesis and malignant progression. order to enhance accuracy efficacy therapeutic outcomes, there is an imminent requirement for vitro models that can accurately replicate intricate characteristics constituents TME. Microfluidic devices exhibit notable advantages investigating progression treatment tumors have potential become novel methodology evaluating immune cell activities TME assist clinicians assessing prognosis patients. addition, it shows great compared traditional experiments. Therefore, review first outlines applications microfluidic chips facilitating culture, constructing activities. Second, roles analysis circulating cells, prognosis, drug screening also been mentioned. Moreover, forward-looking perspective discussed, anticipating widespread clinical adoption future.

Language: Английский

Targeting stromal cells in tumor microenvironment as a novel treatment strategy for glioma DOI Creative Commons

Ziang Geng,

Zheyuan Zhang, Mindy Wang

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 21, 2025

Glioma is the most common primary malignant tumor of central nervous system in adults, characterized by high mortality, low cure rate and recurrence rate. Among gliomas, glioblastoma multiforme (GBM) subtype. Currently, standard treatment for patients with GBM maximum surgical excision combined radiotherapy chemotherapy. But only a small percentage benefit from this treatment. The microenvironment plays an important role occurrence development tumors. It primarily composed cells, peripheral blood vessels, extracellular matrix, signaling molecules, stromal immune cells. cells has emerged as focus current research. interaction among tumor, stromal, within can influence development. Traditional research drug therapy glioma mainly on themselves, but recent studies have found that targeting also modulate progression GBM. Here, we review its related mechanism, well molecular targets pathways, providing new ideas prognosis

Language: Английский

Citations

0
Tumor Microenvironment On‐A‐Chip and Single‐Cell Analysis Reveal Synergistic Stromal–Immune Crosstalk on Breast Cancer Progression DOI Creative Commons
Kalpana Ravi, Yining Zhang,

Lydia Sakala

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Solid tumors develop within a complex environment called the tumor microenvironment (TME), which is sculpted by presence of other cells, such as cancer-associated fibroblasts (CAFs) and immune cells like macrophages (Mφs). Despite orchestrate tumor-supportive through intricate interaction with components TME. However, specific mechanism this intercellular dialogue regulated not fully understood. To that end, development an organotypic 3D breast TME-on-a-chip (TMEC) model, integrated single-cell RNA sequencing analysis, reported to mechanistically evaluate progression triple-negative cancer (TNBC) in patient-derived CAFs Mφs. Extensive functional assays, including invasion morphometric characterization, reveal synergistic influence Mφs on cells. Furthermore, gene expression pathway enrichment analyses identify involvement KYNU gene, suggesting potential evasion kynurenine pathway. Lastly, pharmacological targeting identified investigated.

Language: Английский

Citations

0
Multi-transcriptomics reveals niche-specific expression programs and endothelial cells in glioblastoma DOI Creative Commons
James C. Hu,

Xiaohan Sa,

Yue Yang

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 15, 2025

Glioblastoma (GBM) is a highly lethal malignant intracranial tumor, distinguished from low-grade glioma by histopathological hallmarks such as pseudopalisading cells around necrosis (PAN) and microvascular proliferation (MVP). To date the spatial organization of molecular cellular components these specific features has not been fully elucidated. Here, using bulk RNA sequencing, transcriptomic single cell sequencing (scRNA-seq) data GBM patients, we identified niche-specific transcriptional programs characterized differences in expression between PAN MVP. Notably, discovered spatially distinct domains within tumor core signatures: NDRG1 EPAS1, specifically expressed MVP regions. The clustering results showed two phenotypes endothelial (ECs) were enriched regions, respectively. PAN-associated exhibit copy number variations similar to those cells. Single trajectory analysis reveals pseudotime trajectory, indicating differentiation glioblastoma stem (GSCs) toward ECs. Necrosis cores which are surrounded hypoxic perivascular niches area microenvironment, have considered standardized morphological indicators aggressive GBM. Our findings provide insights into progression.

Language: Английский

Citations

0
Imaging 3D cell cultures with optical microscopy DOI
Huai-Ching Hsieh,

Qinghua Han,

David Brenes

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Language: Английский

Citations

0
Microfabricated Organ-Specific Models of Tumor Microenvironments DOI

Jeong Min Oh,

Yongkuk Park, Jungwoo Lee

et al.

Annual Review of Biomedical Engineering, Journal Year: 2025, Volume and Issue: 27(1), P. 307 - 333

Published: May 1, 2025

Despite the advances in detection, diagnosis, and treatments, cancer remains a lethal disease, claiming lives of more than 600,000 people United States alone 2024. To accelerate development new therapeutic strategies with improved responses, significant efforts have been made to develop microfabricated vitro models tumor microenvironments (TMEs) that address limitations animal-based models. These incorporate several advanced tissue engineering techniques better reflect organ- patient-specific TMEs. Additionally, integrated next-generation single-cell omics technologies provide unprecedented insights into patient's cellular molecular heterogeneity complexity. This review provides an overview recent understanding outlines key TME elements can be captured enhance their physiological relevance. We highlight unique characteristics organs origin or sites dissemination.

Language: Английский

Citations

0
Engineering Heterogeneous Tumor Models for Biomedical Applications DOI Creative Commons
Zhuhao Wu, Danqing Huang, Jinglin Wang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 11(1)

Published: Nov. 9, 2023

Abstract Tumor tissue engineering holds great promise for replicating the physiological and behavioral characteristics of tumors in vitro. Advances this field have led to new opportunities studying tumor microenvironment exploring potential anti‐cancer therapeutics. However, main obstacle widespread adoption models is poor understanding insufficient reconstruction heterogeneity. In review, current progress heterogeneous discussed. First, major components heterogeneity are summarized, which encompasses various signaling pathways, cell proliferations, spatial configurations. Then, contemporary approaches elucidated that guided by fundamental principles biology, a bottom‐up approach highlighted. Additionally, characterization biomedical applications discussed, emphasizing significant role engineered scientific research clinical trials. Lastly, challenges promoting oncology therapy described key directions future provided.

Language: Английский

Citations

7
Recent advances in micro-physiological systems for investigating tumor metastasis and organotropism DOI
Heejeong Yoon, Jonathan Sabaté del Río,

Seung Woo Cho

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1351 - 1366

Published: Jan. 1, 2024

MPS technology holds great potential for studying complex metastasis organotropism. It effectively simulates the dynamic interactions between distinct organ environments and tumor cells, capturing both their inter- intra-tumor heterogeneity.

Language: Английский

Citations

2
Modeling of brain tumors using in vitro, in vivo, and microfluidic models: A review of the current developments DOI Creative Commons

Richu Raju R,

Nour AlSawaftah, Ghaleb A. Husseini

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e31402 - e31402

Published: May 1, 2024

Brain cancers are some of the most complex diseases to treat, despite numerous advances science has made in cancer chemotherapy and research. One key obstacles identifying potential cures for this disease is difficulty emulating complexity brain surrounding microenvironment understand therapeutic approaches. This paper discusses important vitro, vivo, microfluidic tumor models that aim address these challenges.

Language: Английский

Citations

2
Multicompartmentalized microvascularized tumor-on-a-chip to study tumor-stroma interactions and drug resistance in ovarian cancer DOI Open Access
Simona Plesselova,

Kristin Calar,

Hailey Axemaker

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 2, 2024

The majority of ovarian cancer (OC) patients receiving standard care chemotherapy develop chemoresistance within 5 years. tumor microenvironment (TME) is a dynamic and influential player in disease progression therapeutic response. However, there lack models that allow us to elucidate the compartmentalized nature TME controllable, yet physiologically relevant manner its critical role modulating drug resistance.

Language: Английский

Citations

2
Glioma stem cells remodel immunotolerant microenvironment in GBM and are associated with therapeutic advancements DOI
Xifeng Fei, Jie Wu, Haiyan Tian

et al.

Cancer Biomarkers, Journal Year: 2024, Volume and Issue: 41(1), P. 1 - 24

Published: Sept. 3, 2024

Glioma is the most common primary tumor of central nervous system (CNS). Glioblastoma (GBM) incurable with current treatment strategies. Additionally, recurrent GBM (rGBM) often referred to as terminal treatment, necessitating hospice-level care and management. The presence blood-brain barrier (BBB) gives a more challenging or “cold” microenvironment (TME) than that other cancers gloma stem cells (GSCs) play an important role in TME remodeling, occurrence, development recurrence giloma. In this review, our focus will be on discussing following topics: niche-associated GSCs macrophages, new theories regarding GSC involving pyroptosis ferroptosis GBM, metabolic adaptations GSCs, influence cold environment immunotherapy, potential strategies transform into hot one, advancement immunotherapy models.

Language: Английский

Citations

2