A DNA nanostructure‐Hif‐1α inducer complex as novel nanotherapy against cisplatin‐induced acute kidney injury DOI Creative Commons
Yuanchong Chen,

Jiangshan Xu,

Sirong Shi

et al.

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(6)

Published: Jan. 14, 2024

Abstract Since its discovery in 1978, cisplatin‐based chemotherapy regimens have served a pivotal role human cancer treatment, saving millions of lives. However, high risk still poses significant challenge for cisplatin‐induced acute kidney injury (AKI), which occurs 30% cisplatin‐treated patients. Unfortunately, no effective solution preventing or managing this severe complication, greatly impacts clinical administration. Kidney is the main organ injured by cisplatin, and related to cell apoptosis DNA injury. Therefore, achieve safe use cisplatin tumour key lies identifying treatment that can effectively minimize nephrotoxicity. Here, we successfully synthesized applied DNA‐nanostructure complex, named TFG, contains tetrahedral framework nucleic acids (tFNAs) FG‐4592, novel Hif‐1α inducer. As cargo, TFG composed entirely strands. It possesses low nephrotoxicity renal aggregation properties while FG‐4592 able relieve downregulating signal pathways. And it when taken treatment. This work aims enhance protection patients using DNA‐based nanomedicines kidney. has potential revolutionize diseases, particularly drug‐induced injury, leading improved outcomes.

Language: Английский

Typhaneoside-Tetrahedral Framework Nucleic Acids System: Mitochondrial Recovery and Antioxidation for Acute Kidney Injury treatment DOI
Ran Yan, Weitong Cui, Wenjuan Ma

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(9), P. 8767 - 8781

Published: April 14, 2023

Acute kidney injury (AKI) is not only a worldwide problem with cruel hospital mortality rate but also an independent risk factor for chronic disease and promoting its progression. Despite supportive therapeutic measures, there no effective treatment AKI. This study employs tetrahedral framework nucleic acid (tFNA) as vehicle combines typhaneoside (Typ) to develop the tFNA-Typ complex (TTC) treating With precise targeting ability on mitochondria renal tubule, increased antiapoptotic antioxidative effect, promoted function restoration, TTC represents promising nanomedicine AKI treatment. Overall, this has developed dual-targeted nanoparticle enhanced effects could have critical clinical applications in future.

Language: Английский

Citations

81
A DNA tetrahedron-based ferroptosis-suppressing nanoparticle: superior delivery of curcumin and alleviation of diabetic osteoporosis DOI Creative Commons
Yong Li, Zhengwen Cai, Wenjuan Ma

et al.

Bone Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: Feb. 29, 2024

Abstract Diabetic osteoporosis (DOP) is a significant complication that poses continuous threat to the bone health of patients with diabetes; however, currently, there are no effective treatment strategies. In diabetes, increased levels ferroptosis affect osteogenic commitment and differentiation mesenchymal stem cells (BMSCs), leading skeletal changes. To address this issue, we aimed target propose novel therapeutic approach for DOP. We synthesized ferroptosis-suppressing nanoparticles, which could deliver curcumin, natural compound, marrow using tetrahedral framework nucleic acid (tFNA). This delivery system demonstrated excellent curcumin bioavailability stability, as well synergistic properties tFNA. Both in vitro vivo experiments revealed nanoparticles enhance mitochondrial function by activating nuclear factor E2-related 2 (NRF2)/glutathione peroxidase 4 (GPX4) pathway, inhibiting ferroptosis, promoting BMSCs diabetic microenvironment, reducing trabecular loss, increasing formation. These findings suggest curcumin-containing DNA tetrahedron-based have promising potential DOP other ferroptosis-related diseases.

Language: Английский

Citations

74
Myelosuppression Alleviation and Hematopoietic Regeneration by Tetrahedral‐Framework Nucleic‐Acid Nanostructures Functionalized with Osteogenic Growth Peptide DOI Creative Commons
Tianxu Zhang, Mi Zhou, Dexuan Xiao

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(27)

Published: July 26, 2022

As major complications of chemoradiotherapy, myelosuppression and hematopoietic-system damage severely affect immunologic function can delay or even terminate treatment for cancer patients. Although several specific cytokines have been used hematopoiesis recovery, their effect is limited, they may increase the risk tumor recurrence. In this study, osteogenic growth peptide functionalized tetrahedral framework nucleic-acid nanostructures (OGP-tFNAs) are prepared; combine positive stimulating OGP drug carrying tFNAs. The potential OGP-tFNAs hematopoietic stimulation microenvironment regulation investigated. It shown that protect bone marrow stromal cells from 5-fluorouracil (5-FU)-induced DNA apoptosis. pretreatment activates extracellularly regulated protein kinase signal downregulates apoptosis-related proteins. also alleviate chemotherapy-induced inhibition hematopoiesis-related cytokine expression, which crucial reconstitution. conclusion, injuries myelosuppression, while promoting regeneration.

Language: Английский

Citations

72
Functionalized DNA Nanomaterials Targeting Toll‐Like Receptor 4 Prevent Bisphosphonate‐Related Osteonecrosis of the Jaw via Regulating Mitochondrial Homeostasis in Macrophages DOI
Tao Zhang,

Huangshui Ma,

Xiaolin Zhang

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(15)

Published: Jan. 27, 2023

Abstract Imbalance of macrophage polarization characterized by an increase in the percentage pro‐inflammatory M1 macrophages and a decrease anti‐inflammatory M2 is considered critical pathogenic mechanism bisphosphonate‐related osteonecrosis jaws (BRONJ). Because high levels Toll‐like receptor 4 (TLR4) mediates mitochondrial dyshomeostasis Zoledronic Acid (ZA)‐treated macrophages, tetrahedral DNA nanomaterial (TDN)‐modified with TLR4‐siRNA on each vertex (TDN‐TLR4‐4siR) excellent biocompatibility synthesized. This novel TDN‐TLR4‐4siR reverses phenotype imbalance decreasing RAW264.7 macrophages. Mitochondrial dynamics analysis shows shift from short rod‐like ultrastructure to elongated shapes more network continuity ZA‐primed after treatment TDN‐TLR4‐4siR, along elevated expression Mfn1 Mfn2 . further reduces intracellular ROS production restored membrane potential. Furthermore, decreased sequestra formation accelerated healing extraction wound are observed group, resulting incidence rat BRONJ via reprogramming polarized Consequently, this study establishes strategy using regulate homeostasis prevent BRONJ.

Language: Английский

Citations

58
Tetrahedral framework nucleic acids/hyaluronic acid-methacrylic anhydride hybrid hydrogel with antimicrobial and anti-inflammatory properties for infected wound healing DOI Creative Commons

Qi Cai,

Qiang Sun, Dexuan Xiao

et al.

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: April 16, 2024

Abstract Bacterial resistance and excessive inflammation are common issues that hinder wound healing. Antimicrobial peptides (AMPs) offer a promising versatile antibacterial option compared to traditional antibiotics, with additional anti-inflammatory properties. However, the applications of AMPs limited by their antimicrobial effects stability against bacterial degradation. TFNAs regarded as drug delivery platform could enhance properties nanodrugs. Therefore, in this study, composite hydrogel (HAMA/t-GL13K) was prepared via photocross-linking method, which tFNAs carry GL13K. The injectable, biocompatible, be instantly photocured. It exhibited broad-spectrum inhibiting expression inflammatory factors scavenging ROS. Thereby, inhibited infection, shortened healing time skin defects infected full-thickness defect models reduced scarring. constructed HAMA/tFNA-AMPs hydrogels exhibit potential for clinical use treating microbial infections promoting

Language: Английский

Citations

36
Framework Nucleic Acid-Based and Neutrophil-Based Nanoplatform Loading Baicalin with Targeted Drug Delivery for Anti-Inflammation Treatment DOI
Mi Zhou,

Yuanlin Tang,

Yifei Lu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Targeted drug delivery is a promising strategy for treating inflammatory diseases, with recent research focusing on the combination of neutrophils and nanomaterials. In this study, targeted nanodrug platform (Ac-PGP-tFNA, APT) was developed using tetrahedral framework nucleic acid (tFNA) along neutrophil hitchhiking mechanism to achieve precise anti-inflammatory effects. The tFNA structure, known its excellent drug-loading capacity cellular uptake efficiency, used carry therapeutic agent─baicalin. results demonstrate that development not only considerably enhances bioavailability effective concentration (baicalin) but also promotes polarization pro-inflammatory M1 macrophages M2 by modulating interactions between macrophages. This method effectively treats conditions such as sepsis introduces managing diseases characterized infiltration.

Language: Английский

Citations

9
Bacterial carrier-mediated drug delivery systems: a promising strategy in cancer therapy DOI Creative Commons
Shaomin Yan, Yu Gan, Huizhe Xu

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 8, 2025

Cancer is a major killer threatening modern human health and leading cause of death worldwide. Due to the heterogeneity complexity cancer, traditional treatments have limited effectiveness. To address this problem, an increasing number researchers medical professionals are working develop new ways treat cancer. Bacteria chemotaxis that can target colonize tumor tissue, as well activate anti-tumor immune responses, which makes them ideal for biomedical applications. With rapid development nanomedicine synthetic biology technologies, bacteria extensively used carriers drug delivery tumors, holds promise overcoming limitations conventional cancer treatment regimens. This paper summarizes examples anti-cancer drugs delivered by bacterial carriers, their strengths weaknesses. Further, we emphasize carrier systems in clinical translation.

Language: Английский

Citations

2
Current Understanding and Translational Prospects of Tetrahedral Framework Nucleic Acids DOI Creative Commons
Junjie Gu,

Jiale Liang,

Taoran Tian

et al.

JACS Au, Journal Year: 2025, Volume and Issue: 5(2), P. 486 - 520

Published: Feb. 10, 2025

Tetrahedral framework nucleic acids (tFNAs) represent a promising advancement in acid nanotechnology due to their unique structural properties, high biocompatibility, and multifaceted biomedical applications. Constructed through one-pot annealing method, four single-stranded DNAs self-assemble into stable, three-dimensional tetrahedral nanostructures with enhanced mechanical robustness physiological stability, resisting enzymatic degradation. Their ability permeate mammalian cells without transfection agents, coupled modifiable surfaces, positions tFNAs as versatile carriers for drug gene delivery systems. The tFNA-based platforms exhibit superior therapeutic efficacy, including antioxidative anti-inflammatory effects, alongside efficient cellular uptake tissue penetration. These features underpin role precision medicine, enabling targeted of diverse agents such synthetic compounds, peptides, acids. Additionally, demonstrate significant potential regenerative immune modulation, antibacterial strategies, oncology. By addressing challenges translational integration, stand poised accelerate the development research clinical applications, fostering novel therapies enhancing outcomes across wide spectrum diseases. This Perspective thoroughly details attributes applications critically evaluates tFNAs' potential, outlining inherent implementation exploring solutions these obstacles.

Language: Английский

Citations

2
Effects of Puerarin‐Loaded Tetrahedral Framework Nucleic Acids on Osteonecrosis of the Femoral Head DOI
Yuxuan Zhao, Songhang Li,

Maogeng Feng

et al.

Small, Journal Year: 2023, Volume and Issue: 19(41)

Published: June 14, 2023

Osteonecrosis of the femoral head (ONFH) is recognized as a common refractory orthopedic disease that causes severe pain and poor quality life in patients. Puerarin (Pue), natural isoflavone glycoside, can promote osteogenesis inhibit apoptosis bone mesenchymal stem cells (BMSCs), demonstrating its great potential treatment osteonecrosis. However, low aqueous solubility, fast degradation vivo, inadequate bioavailability, limit clinical application therapeutic efficacy. Tetrahedral framework nucleic acids (tFNAs) are promising novel DNA nanomaterials drug delivery. In this study, tFNAs Pue carriers used synthesized tFNA/Pue complex (TPC) exhibited better stability, biocompatibility, tissue utilization than free Pue. A dexamethasone (DEX)-treated BMSC model vitro methylprednisolone (MPS)-induced ONFH vivo also established, to explore regulatory effects TPC on BMSCs. This findings showed restore dysfunction attenuated induced by high-dose glucocorticoids (GCs) through hedgehog Akt/Bcl-2 pathways, contributing prevention GC-induced rats. Thus, for other osteogenesis-related diseases.

Language: Английский

Citations

41
Amelioration of Osteoarthritis via Tetrahedral Framework Nucleic Acids Delivering Microrna‐124 for Cartilage Regeneration DOI Open Access
Sirong Shi, Tianyu Chen, Weitong Lu

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(46)

Published: July 9, 2023

Abstract MicroRNAs (miRNAs) regulate several physiological and pathological processes involved in various diseases, including osteoarthritis (OA). OA is the most common global musculoskeletal disorder, characterized by irreversible progressive destruction of articular cartilage. Supplementation with exogenous miRNAs may represent a promising therapeutic treatment, miRNA‐124 (miR‐124) being prime candidate for its anti‐inflammatory ability; however, an effective drug delivery system urgently required to enhance miR‐124 stability capacity enter chondrocytes. To this end, tetrahedral framework nucleic acids’ (tFNAs) self‐assembled 3D DNA nanostructures possess superior inherent biocompatibility, versatile functionality, unsurpassed editability, strong cellular internalization ability. In study, tFNAs carrying one or three (T‐miR1 T‐miR3) are successfully synthesized. T‐miR3 largely absorbed via induced inflammatory chondrocytes IL‐1β. With reactive oxygen species’ scavenging ability inflammation‐suppressive release behavior, efficiently protects against IL‐1β injury vitro. Additionally, effectively prevents progression inhibiting chondrocyte apoptosis, smoothing cartilage surfaces, suppressing extracellular matrix degradation, increasing synovial thickness, protecting vivo cartilage, illustrating treatment. This study provides experimental evidence novel strategies treatment clinical setting.

Language: Английский

Citations

33