Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 493, P. 152736 - 152736
Published: June 1, 2024
Language: Английский
Bone Research, Journal Year: 2024, Volume and Issue: 12(1)
Published: Feb. 29, 2024
Abstract Diabetic osteoporosis (DOP) is a significant complication that poses continuous threat to the bone health of patients with diabetes; however, currently, there are no effective treatment strategies. In diabetes, increased levels ferroptosis affect osteogenic commitment and differentiation mesenchymal stem cells (BMSCs), leading skeletal changes. To address this issue, we aimed target propose novel therapeutic approach for DOP. We synthesized ferroptosis-suppressing nanoparticles, which could deliver curcumin, natural compound, marrow using tetrahedral framework nucleic acid (tFNA). This delivery system demonstrated excellent curcumin bioavailability stability, as well synergistic properties tFNA. Both in vitro vivo experiments revealed nanoparticles enhance mitochondrial function by activating nuclear factor E2-related 2 (NRF2)/glutathione peroxidase 4 (GPX4) pathway, inhibiting ferroptosis, promoting BMSCs diabetic microenvironment, reducing trabecular loss, increasing formation. These findings suggest curcumin-containing DNA tetrahedron-based have promising potential DOP other ferroptosis-related diseases.
Language: Английский
Citations
74
Advanced Science, Journal Year: 2024, Volume and Issue: 11(21)
Published: March 9, 2024
Abstract Angiogenesis is crucial for tissue engineering, wound healing, and regenerative medicine. Nanomaterials constructed based on specific goals can be employed to activate endogenous growth factor‐related signaling. In this study, the conventional single‐stranded DNA self‐assembly into tetrahedral framework nucleic acids (tFNAs), Apt02 acid aptamer dimethyloxallyl glycine (DMOG) small molecule are integrated a complex via template‐based click chemistry reaction toehold‐mediated strand displacement reaction. Thus, being able simulate VEGF (vascular endothelial factor) function stabilize HIF (hypoxia‐inducible factor), functional whole applied angiogenesis. Cellular studies demonstrate that tFNAs‐Apt02 (TAC) has conspicuous affinity human umbilical vein cells (HUVECs). Further incubation with DMOG yields tFNAs‐Apt02‐DMOG (TACD), which promotes secretion, in vitro blood vessel formation, sprouting, migration of HUVECs. Additionally, TACD enhances angiogenesis by upregulating VEGF/VEGFR signaling pathways. Moreover, diabetic mouse skin defect repair process, increases formation collagen deposition, therefore accelerating healing. The novel strategy simulating stabilizing blood‐vessel vivo potential broad applications vascularization field.
Language: Английский
Citations
37
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(28)
Published: March 12, 2024
Abstract Diabetes mellitus (DM) is characterized by elevated blood glucose and advanced glycation end product (AGEs) levels. Increased AGEs in bone tissue inhibit osteogenic differentiation marrow mesenchymal stem cells (BMSCs), leading to loss osteoporosis diabetic patients. Enhancing the capacity of BMSCs presence abundant can improve health prevent DM The flavonoid, Quercetin, has anti‐inflammatory, antibacterial, antitumor properties; however, it insoluble water thus not easily absorbed body. Nanodrug delivery systems such as tetrahedral framework nucleic acids (tFNAs) exhibit excellent biocompatibility, efficient cell uptake, drug piggybacking. In present study, tFNAs with quercetin complexed form a novel nanodrug (tFNAs/Que) that combined features both components. tFNAs/Que promote an vitro AGEs‐rich environment, maintain mass, mice vivo. mechanism against may be related JNK signaling pathway. conclusion, shown dual regulatory role promoting inhibiting apoptosis. Such feature promising for prevention treatment osteoporosis.
Language: Английский
Citations
31
Advanced Science, Journal Year: 2024, Volume and Issue: 11(17)
Published: Feb. 14, 2024
Abstract Hydrocephalus is one of the most common brain disorders and a life‐long incurable condition. An empirical “one‐size‐fits‐all” approach cerebrospinal fluid (CSF) shunting remains mainstay hydrocephalus treatment effective pharmacotherapy options are currently lacking. Macrophage‐mediated ChP inflammation CSF hypersecretion have recently been identified as significant discovery in pathogenesis hydrocephalus. In this study, pioneering DNA nano‐drug (TSOs) developed by modifying S2 ssDNA S4 with SPAK ASO OSR1 tetrahedral framework nucleic acids (tFNAs) synthesis via one‐pot annealing procedure. This construct can significantly knockdown expression OSR1, along their downstream ion channel proteins epithelial cells, thereby leading to decrease secretion. Moreover, these findings indicate that TSOs effectively inhibit M0 M1 phenotypic switch macrophages MAPK pathways, thus mitigating cytokine storm. vivo post‐hemorrhagic (PHH) models, reduce secretion rates, alleviate inflammation, prevent onset These compelling results highlight potential promising therapeutic option for managing hydrocephalus, applications future.
Language: Английский
Citations
25
Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 191 - 205
Published: May 21, 2024
Unnecessary exposure to ionizing radiation (IR) often causes acute and chronic oxidative damages normal cells organs, leading serious physiological even life-threatening consequences. Amifostine (AMF) is a validated radioprotectant extensively applied in chemotherapy medicine, but the short half-life limits its bioavailability clinical applications, remaining as great challenge be addressed. DNA-assembled nanostructures especially tetrahedral framework nucleic acids (tFNAs) are promising nanocarriers with preeminent biosafety, low biotoxicity, high transport efficiency. The tFNAs also have relative long-term maintenance for structural stability excellent endocytosis capacity. We therefore synthesized tFNA-based delivery system of AMF multi-organ radioprotection (tFNAs@AMF, termed nanosuit). By establishing mice models accidental total body irradiation (TBI) radiotherapy model Lewis lung cancer, we demonstrated that nanosuit could shield from IR-induced DNA damage by regulating molecular biomarkers anti-apoptosis anti-oxidative stress. In model, pretreated exhibited satisfactory alteration superoxide dismutase (SOD) activities malondialdehyde (MDA) contents, functional recovery hematopoietic system, reducing pathological safeguarding lethal radiation. More importantly, showed selective organs without interferences tumor control cancer. Based on conveniently available tetrahedron-based nanocarrier, this work presents high-efficiency prolonged enhanced multi-organs. Such pioneers strategy translation potential radioactivity protection.
Language: Английский
Citations
21
JACS Au, Journal Year: 2025, Volume and Issue: 5(2), P. 486 - 520
Published: Feb. 10, 2025
Tetrahedral framework nucleic acids (tFNAs) represent a promising advancement in acid nanotechnology due to their unique structural properties, high biocompatibility, and multifaceted biomedical applications. Constructed through one-pot annealing method, four single-stranded DNAs self-assemble into stable, three-dimensional tetrahedral nanostructures with enhanced mechanical robustness physiological stability, resisting enzymatic degradation. Their ability permeate mammalian cells without transfection agents, coupled modifiable surfaces, positions tFNAs as versatile carriers for drug gene delivery systems. The tFNA-based platforms exhibit superior therapeutic efficacy, including antioxidative anti-inflammatory effects, alongside efficient cellular uptake tissue penetration. These features underpin role precision medicine, enabling targeted of diverse agents such synthetic compounds, peptides, acids. Additionally, demonstrate significant potential regenerative immune modulation, antibacterial strategies, oncology. By addressing challenges translational integration, stand poised accelerate the development research clinical applications, fostering novel therapies enhancing outcomes across wide spectrum diseases. This Perspective thoroughly details attributes applications critically evaluates tFNAs' potential, outlining inherent implementation exploring solutions these obstacles.
Language: Английский
Citations
2
Bioactive Materials, Journal Year: 2023, Volume and Issue: 33, P. 279 - 310
Published: Nov. 24, 2023
DNA not only plays a vital role in nature as fundamental hereditary material for storing genetic material, but also serves well-defined functional example, building blocks the assembly of nanoscale bio-architectures by Watson-Crick base-pairing interaction. With development molecular biology, biotechnology and nanoscience, structural nanotechnology has achieved numerous advances, contributing to construction various nanostructures ranging from discrete objects one dimensional (1D), two (2D), three (3D) architectures. Among them, tetrahedral nanoarchitecture is intensively studied because simple 3D structure, easy design unique properties, such high rigidity, desirable biostability efficient cellular uptake without auxiliary species. This review summarizes research progress outlines applications biosensing, drug delivery targeted therapy. Moreover, dependence biological activity biomolecules on tetrahedron-mediated spatially-controlled arrangement great potential are discussed. In addition, challenges clinic tetrahedron-based platforms described, perspectives towards biomedical foreseen, our understandings further studies tetrahedron provided, aiming motivate interdisciplinary research.
Language: Английский
Citations
34
ACS Nano, Journal Year: 2023, Volume and Issue: 17(22), P. 22668 - 22683
Published: Sept. 26, 2023
Complications arising from diabetes can threaten multiple organs. Advanced glycation end products (AGEs) play a significant role in inducing these complications. Highly processed diets and hyperglycemia facilitate the accumulation of AGEs body. Interaction between their main receptor (RAGE) initiates transmission intracellular inflammatory cell death signals, which ultimately lead to To counter AGEs-induced damage, we developed an siRNA-binding tetrahedral framework nucleic acids (TDN) system, termed Tsi, combines potent membrane penetrability serum stability TDN with gene-targeting specificity siRNA-RAGE. Tsi effectively persistently downregulates expression RAGE, thereby suppressing inflammation by blocking NF-κB pathway as well exhibiting antioxidant functions. Furthermore, regulates pyroptosis state macrophages via NLRP3/caspase-1 axis, inhibits spread signals maintains homeostasis. This is great significance for synergistic treatment strategy systemic complications patients refractory hyperglycemia. In summary, this study describes nanomedicine that targets RAGE suppresses AGE-induced inflammation. acid drug holds long-lasting efficacy independent lowering hyperglycemia, provides diabetic age-related diseases.
Language: Английский
Citations
28
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: April 1, 2024
Abstract Background Herbal nanoparticles are made from natural herbs/medicinal plants, their extracts, or a combination with other nanoparticle carriers. Compared to traditional herbs, herbal lead improved bioavailability, enhanced stability, and reduced toxicity. Previous research indicates that medicine nanomaterials rapidly advancing making significant progress; however, bibliometric analysis knowledge mapping for currently lacking. We performed by retrieving publications related the Web of Science Core Collection (WoSCC) database spanning 2004 2023. Data processing was using R package Bibliometrix, VOSviewers, CiteSpace. Results In total, 1876 articles were identified, originating various countries, China being primary contributing country. The number in this field increases annually. Beijing University Chinese Medicine, Shanghai Traditional Saveetha India prominent institutions domain. Journal “International Nanomedicine” has highest publications. authors these reached 8234, Yan Zhao, Yue Zhang, Huihua Qu most prolific Zhao frequently cited author. “Traditional medicine,” “drug delivery,” “green synthesis” main focal points. Themes such as synthesis,” “curcumin,” “wound healing,” “carbon dots” may represent emerging areas. Conclusions Our study findings assist identifying latest frontiers hot topics, providing valuable references scholars investigating role nanotechnology medicine. Graphical
Language: Английский
Citations
10
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(26), P. 33192 - 33204
Published: June 17, 2024
The human body's primary line of defense, the skin, is especially prone to harm. Although microRNA (miRNA)-based therapies have attracted increasing attention for skin wound healing, their applications remain limited owing a range issues. Tetrahedral framework DNA (tFNA), nanomaterial possessing nucleic acid characteristics, exhibits an excellent biocompatibility, in addition anti-inflammatory and transdermal delivery capabilities, can accelerate healing. Due its potential exert synergistic action with therapeutic miRNA, tFNA has been considered ideal vehicle miRNA therapy. design synthesis bioswitchable system (BiRDS) reported, which contains three miRNAs as well core maximize loading capacity while preserving characteristics tFNA. A high stability, permeability cells tissues good biological compatibility are demonstrated. By selectively inhibiting heparin-binding epidermal growth factor (HB-EGF), BiRDS inhibit NF-κB pathway simultaneously controlling PTEN/Akt pathway. As result, helps healing go through inflammation proliferative phase. This study demonstrates advantages miRNA-based therapy provides new research ideas treatment skin-related diseases.
Language: Английский
Citations
10