An Immunomodulatory Hydrogel by Hyperthermia‐Assisted Self‐Cascade Glucose Depletion and ROS Scavenging for Diabetic Foot Ulcer Wound Therapeutics

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(48)

Published: Oct. 7, 2023

Abstract Current therapeutic protocols for diabetic foot ulcers (DFUs), a severe and rapidly growing chronic complication in patients, remain nonspecific. Hyperglycemia‐caused inflammation excessive reactive oxygen species (ROS) are common obstacles encountered DFU wound healing, often leading to impaired recovery. These two effects reinforce each other, forming an endless loop. However, adequate inclusive methods still lacking target these aspects break the vicious cycle. This study proposes novel approach treating wounds, utilizing immunomodulatory hydrogel achieve self‐cascade glucose depletion ROS scavenging regulate microenvironment. Specifically, AuPt@melanin‐incorporated (GHM3) dressing is developed facilitate efficient hyperthermia‐enhanced local scavenging. Mechanistically, vitro/vivo experiments RNA sequencing analysis demonstrate that GHM3 disrupts ROS‐inflammation cascade cycle downregulates ratio of M1/M2 macrophages, consequently improving outcomes dorsal skin wounds rats. In conclusion, this proposed offers facile, safe, highly treatment modality DFUs.

Language: Английский

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All‐Natural Immunomodulatory Bioadhesive Hydrogel Promotes Angiogenesis and Diabetic Wound Healing by Regulating Macrophage Heterogeneity

Advanced Science, Journal Year: 2023, Volume and Issue: 10(13)

Published: March 2, 2023

Abstract Macrophages are highly heterogeneous and exhibit a diversity of functions phenotypes. They can be divided into pro‐inflammatory macrophages (M1) anti‐inflammatory (M2). Diabetic wounds characterized by prolonged inflammatory phase difficulty in healing due to the accumulation wound. Therefore, hydrogel dressings with macrophage heterogeneity regulation function hold great promise promoting diabetic wound clinical applications. However, precise conversion M1 M2 simple biosafe approaches is still challenge. Here, an all‐natural ability regulate developed promote angiogenesis healing. The protocatechuic aldehyde hybridized collagen‐based exhibits good bioadhesive antibacterial properties as well reactive oxygen species scavenging ability. More importantly, able convert without need for any additional ingredients or external intervention. This safe immunomodulatory approach shows application potential shortening repair accelerating

Language: Английский

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Developing natural polymers for skin wound healing

Bioactive Materials, Journal Year: 2023, Volume and Issue: 33, P. 355 - 376

Published: Nov. 26, 2023

Natural polymers are complex organic molecules that occur in the natural environment and have not been subjected to artificial synthesis. They frequently encountered various creatures, including mammals, plants, microbes. The aforementioned commonly derived from renewable sources, possess a notable level of compatibility with living organisms, limited adverse effect on environment. As result, they hold considerable significance development sustainable environmentally friendly goods. In recent times, there has advancement investigation potential uses field biomedicine, specifically relation biomaterials exhibit antibacterial antioxidant characteristics. This review provides comprehensive overview prevalent utilized biomedical domain throughout preceding two decades. this paper, we present examination components typical methods for preparation based polymers. Furthermore, summarize application polymer materials each stage skin wound repair. Finally, key findings insights into limitations current elucidate prospects their future field.

Language: Английский

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Smart‐Responsive Multifunctional Therapeutic System for Improved Regenerative Microenvironment and Accelerated Bone Regeneration via Mild Photothermal Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 11(2)

Published: Nov. 7, 2023

Abstract The treatment of bone defects remains a substantial clinical challenge due to the lack spatiotemporal management immune microenvironment, revascularization, and osteogenic differentiation. Herein, deferoxamine (DFO)‐loaded black phosphorus nanosheets decorated by polydopamine layer are prepared (BPPD) compounded into gelatin methacrylate/sodium alginate methacrylate (GA) hybrid hydrogel as smart‐responsive therapeutic system (GA/BPPD) for accelerated regeneration. BPPD nanocomposites served bioactive components near‐infrared (NIR) photothermal agents, which conferred with excellent NIR/pH dual‐responsive properties, realizing stimuli‐responsive release DFO PO 4 3 − during Under action NIR‐triggered mild therapy, GA/BPPD exhibited positive effect on promoting osteogenesis angiogenesis, eliminating excessive reactive oxygen species, inducing macrophage polarization M2 phenotype. More significantly, through polarization‐induced osteoimmune this platform could also drive functional cytokine secretion enhanced angiogenesis osteogenesis. In vivo experiments further demonstrated that facilitate healing attenuating local inflammatory response, increasing pro‐healing factors, stimulating endogenous cell recruitment, accelerating revascularization. Collectively, proposed intelligent provides promising strategy reshape damaged tissue microenvironment augmented

Language: Английский

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An Immunoregulation Hydrogel with Controlled Hyperthermia‐Augmented Oxygenation and ROS Scavenging for Treating Diabetic Foot Ulcers

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(33)

Published: May 6, 2024

Abstract Diabetic foot ulcers (DFUs), a serious and increasingly common chronic issue among diabetics, often do not respond well to generalized treatment strategies. Hypoxia the overexpression of reactive oxygen species (ROS), resulting in inflammatory dysregulation subsequent imbalance macrophage phenotypes, are critical factors contributing prolonged non‐healing DFU wounds. These two issues interact continuous, problematic cycle. Presently, there is lack comprehensive strategies aimed at addressing both these simultaneously interrupt this detrimental Herein, an immunomodulatory hydrogel (PHG2) developed for reshaping hostile microenvironment. The engineered PHG2 only removes excess internally‐produced ROS but also generates O 2 , with its efficiency further boosted by local hyperthermia (42.5 °C) activated near‐infrared light. Through vitro vivo studies, along transcriptomic assessment, it confirmed that disrupts feedback loop between inflammation while lowering M1/M2 ratio. Such discoveries contribute significant enhancement healing process injuries undergo gradual increase movement, covering wounds from back, mouth, foot. Ultimately, method provides easy, safe, highly effective solution treating DFUs.

Language: Английский

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Self‐Healing, Injectable Hydrogel Dressing for Monitoring and Therapy of Diabetic Wound

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(36)

Published: Feb. 28, 2024

Abstract Chronic diabetic wound repair is a global medical challenge. Developing hydrogel dressing to effectively promote angiogenesis and detect state in management an unmet requirement. Herein, facile efficient strategy prepare self‐healing with enhanced real‐time monitoring for wounds presented, by cross‐linking carboxymethyl cellulose (CMC) precoordinated europium‐ethylenediaminetetraacetic acid (Eu‐EDTA) complexes. Benefiting from the dynamic reversible coordination interaction formation, exhibits remarkable injectability properties, thus forming coverage irregular deep minimally invasive procedures. Furthermore, fluorescence of changes linearly pH value range 4.5–7.5 enables measurement monitor state. More importantly, can proliferation migration endothelial cells enhance tube formation ability, demonstrating great capacity tissue remodeling. The vivo experiments manifested that upregulating expression angiogenesis‐related factors downregulating matrix metalloproteinase‐9 (MMP‐9), contributing granulation collagen deposition, repair. This work provide promising diagnosis therapy clinical wounds.

Language: Английский

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An Antioxidative and Active Shrinkage Hydrogel Integratedly Promotes Re‐Epithelization and Skin Constriction for Enhancing Wound Closure

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)

Published: Feb. 9, 2024

Abstract Delayed re‐epithelization and weakened skin contractions are the two primary factors that hinder wound closure in large‐scale acute or chronic wounds. However, effective strategies for targeting these aspects concurrently still lacking. Herein, an antioxidative active‐shrinkage hydrogel (AHF@AS Gel) is constructed can integratedly promote constriction to accelerate diabetic closure. The AHF@AS Gel encapsulated by amino‐ hydroxyl‐modified C 70 fullerene (AHF) a thermosensitive active shrinkage (AS Gel). Specifically, AHF relieves overactivated inflammation, prevents cellular apoptosis, promotes fibroblast migration vitro reducing excessive reactive oxygen species (ROS). Notably, achieved ≈2.7‐fold ≈1.7‐fold better wounds wounds, respectively, significantly contributing promotion of Using proteomic profiling mechanistic studies, it identified efficiently promoted transition inflammatory proliferative phases remodeling phase. demonstrated AS alone activates mechanosensitive epidermal growth factor receptor/Akt (EGFR/Akt) pathway cell proliferation. offers comprehensive strategy via biochemistry regulation integrating with mechanical forces stimulation.

Language: Английский

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Three‐Step Regenerative Strategy: Multifunctional Bilayer Hydrogel for Combined Photothermal/Photodynamic Therapy to Promote Drug‐Resistant Bacteria‐Infected Wound Healing

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(2)

Published: Sept. 27, 2023

Abstract The drug‐resistant bacterial‐infected skin wound is still a severe healthcare problem. Uncontrolled bacterial infection, abundant reactive oxygen species (ROS) content, and prolonged inflammatory response are detrimental to healing. Moreover, excessive vessel growth can result in unsatisfactory scar formation. Herein, three‐step regenerative strategy based on bilayered gelatin/acryloyl β ‐cyclodextrin (BGACD) hydrogel containing physical host–guest complexations chemical crosslinks proposed. loaded with humic acids (HAs) astragaloside IV (AS) the lower layer verteporfin (Vt) upper layer. Different ratios endow layers of different degradation rates. Under light irradiation, combination HAs‐induced photothermal therapy (PTT) verteporfin‐induced photodynamic effectively inhibits MRSA growth. HAs released from scavenge ROS promote M2 macrophage polarization angiogenesis during inflammation early proliferation phases, while releases suppress late remodeling phases. HAs‐AS@Vt@BGACD successfully achieves rapid scarless healing an MRSA‐infected model rats. Therefore, shows promising potential for treatment bacteria‐infected

Language: Английский

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A Double Network Composite Hydrogel with Self-Regulating Cu²⁺/Luteolin Release and Mechanical Modulation for Enhanced Wound Healing

ACS Nano, Journal Year: 2024, Volume and Issue: 18(26), P. 17251 - 17266

Published: June 22, 2024

Designing adaptive and smart hydrogel wound dressings to meet specific needs across different stages of healing is crucial. Here, we present a composite hydrogel, GSC/PBE@Lut, that offers self-regulating release cupric ions luteolin modulates mechanical properties promote chronic healing. The double network GSC, fabricated through photo-cross-linking gelatin methacrylate, followed by Cu2+-alginate coordination cross-linking. On one hand, GSC allows for rapid Cu2+ eliminate bacteria in the acidic pH environment during inflammation reduces hydrogel's strength minimize tissue trauma early dressing changes. other enables slow proliferation stage, promoting angiogenesis biocompatibility. Furthermore, inclusion pH- reactive oxygen species (ROS)-responsive nanoparticles (PBE@Lut) matrix controlled luteolin, offering antioxidant anti-inflammatory effects macrophage polarization. In murine model Staphylococcus aureus infected wounds, GSC/PBE@Lut demonstrates exceptional therapeutic benefits antibacterial, anti-inflammatory, angiogenic, regeneration. Overall, our results suggest hydrogels with bioactive agent modulation promising solution treating wounds.

Language: Английский

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Chitosan-based hydrogel dressings for diabetic wound healing via promoting M2 macrophage-polarization

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 331, P. 121873 - 121873

Published: Jan. 30, 2024

Language: Английский

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