Self-assembled carrier-free formulations based on medicinal and food active ingredients DOI
Hao Yuan,

Haixia Ji,

Li Gao

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The popularity of medicinal plants, which have a unique system and are mostly used in compound form for the prevention treatment wide range diseases, is growing worldwide. In recent years, with advances chemical separation structural analysis techniques, many major bioactive molecules plants been identified. However, active ingredients often possess characteristics, including poor water solubility, stability bioavailability, limit their therapeutic applications. To address this problem, self-assembly small from food sources provides new strategy. Driven by various types acting forces, modifiable groups, multiple sites action, hydrophobic side chains, rigid backbones properties able to supramolecular network hydrogels, nanoparticles, micelles, other self-assemblies. This review first summarizes forms self-assemblies such as micelles at level action site, discusses studies on that can be self-assembly, addition summarizing advantages variety disease applications, wound healing, antitumor, anticancer, diabetes mellitus. Finally, problems self-assemblers possible directions future development presented. We firmly believe potential develop effective compounds drug-food homologous providing valuable information drug research strategies perspectives modernization Chinese medicine.

Language: Английский

Hypoxia causes trophoblast cell ferroptosis to induce miscarriage through lnc-HZ06/HIF1α-SUMO/NCOA4 axis DOI Creative Commons
Peng Tian,

Zhongyan Xu,

Jiarong Guo

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 70, P. 103073 - 103073

Published: Feb. 2, 2024

Defects of human trophoblast cells may induce miscarriage (abnormal early embryo loss), which is generally regulated by lncRNAs. Ferroptosis a newly identified iron-dependent programmed cell death. Hypoxia an important and unavoidable feature in mammalian cells. However, whether hypoxia might ferroptosis then miscarriage, as well lncRNA, was completely unknown. In this work, we discovered at the first time that could result miscarriage. We also novel lncRNA (lnc-HZ06) simultaneously (indicated HIF1α protein), ferroptosis, mechanism, HIF1α-SUMO, instead itself, primarily acted transcription factor to promote NCOA4 (ferroptosis indicator) hypoxic Lnc-HZ06 promoted SUMOylation suppressing SENP1-mediated deSUMOylation. HIF1α-SUMO lnc-HZ06 transcription. Thus, both formed positive auto-regulatory feedback loop. This loop up-regulated cells, RM villous tissues, placental tissues hypoxia-treated mice, further induced up-regulating HIF1α-SUMO-mediated Furthermore, knockdown either murine lnc-hz06 or Ncoa4 efficiently suppress alleviate mouse model. Taken together, study provided new insights understanding regulatory roles lnc-HZ06/HIF1α-SUMO/NCOA4 axis among hypoxia, offered effective approach for treatment against

Language: Английский

Citations

18
Defective Homologous Recombination Repair By Up‐Regulating Lnc‐HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage DOI Creative Commons
Weina Chen,

Chenyang Mi,

Ying Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)

Published: Jan. 29, 2024

Abstract Human trophoblast cells are crucial for healthy pregnancy. However, whether the defective homologous recombination (HR) repair of dsDNA break (DSB) in may induce miscarriage is completely unknown. Moreover, abundance BRCA1 (a protein HR repair), its recruitment to DSB foci, and epigenetic regulatory mechanisms, also fully unexplored. In this work, it identified that a novel lnc‐HZ10, which highly experssed villous tissues recurrent (RM) vs their control group, suppresses cell. Lnc‐HZ10 AhR (aryl hydrocarbon receptor) form positive feedback loop. acts as transcription factor promote lnc‐HZ10 transcription. Meanwhile, increases levels by suppressing CUL4B‐mediated ubiquitination degradation. Subsequently, transcription; (mainly 1‐447 nt) interacts with γ‐H2AX; thus, impairs interactions BRCA1. BPDE exposure trigger loop suppress cells, possibly inducing miscarriage. Knockdown murine Ahr efficiently recovers placental alleviates mouse model. Therefore, suggested AhR/lnc‐HZ10/BRCA1 axis be promising target alleviation unexplained

Language: Английский

Citations

7
BaP/BPDE suppresses human trophoblast cell migration/invasion and induces unexplained miscarriage by up-regulating a novel lnc-HZ11 in extracellular vesicles: An intercellular study DOI Creative Commons

Chenyang Mi,

Weina Chen, Ying Zhang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 188, P. 108750 - 108750

Published: May 21, 2024

Extracellular vesicles (EVs) mediate the intercellular crosstalk by transferring functional cargoes. Recently, we have discovered that BaP/BPDE exposure suppresses trophoblast cell migration/invasion and induces miscarriage, which are also regulate lncRNAs at intracelluar levels. However, EVs-mediated regulatory mechanisms completely unexplored. Specifically, whether EVs might transfer BPDE-induced toxic lncRNA to fresh recipient cells suppress their further induce miscarriage is unknown. In this study, find BPDE up-regulates a novel lnc-HZ11, EGR1/NF-κB/CXCL12 pathway of cells. Intercellular studies show EV-HZ11 (lnc-HZ11 in EVs), highly expressed BPDE-exposed donor cells, lnc-HZ11 through EVs. Analysis villous tissues collected from UM (unexplained miscarriage) patients HC (healthy control) group shows levels BPDE-DNA adducts, or EV-lnc-HZ11, all associated with miscarriage. Mouse assays BaP lnc-Hz11 EV-Hz11, Egr1/Nf-κb/Cxcl12 pathway, eventually Knockdown injecting EV-AS-Hz11 could effectively alleviate BaP-exposed mice. Furthermore, serum samples well predict risk Collectively, study not only discovers EVs-HZ11-mediated but provides new approach for treatment against unexplained EV-HZ11.

Language: Английский

Citations

3
BaP/BPDE exposure causes human trophoblast cell dysfunctions and induces miscarriage by up-regulating lnc-HZ06-regulated IL1B DOI
Jiarong Guo, Jingsong Zhao, Peng Tian

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 476, P. 134741 - 134741

Published: June 12, 2024

Language: Английский

Citations

3
Lnc-HZ06 down-regulates HIF1α protein levels in CoCl2-exposed hypoxic trophoblast cells and villous tissues of miscarriage patients DOI
Peng Tian, Jingsong Zhao, Jiarong Guo

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 978, P. 179477 - 179477

Published: April 21, 2025

Language: Английский

Citations

0
Organ-specific lithium accumulation and its toxic effects on female reproduction DOI

Qigang Fan,

Yi Sun, Qikun Shen

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 138516 - 138516

Published: May 1, 2025

Language: Английский

Citations

0
BaP/BPDE suppresses homologous recombination repair in human trophoblast cells to induce miscarriage: The roles of lnc-HZ08 DOI Creative Commons
Weina Chen, Chenglong Ma, Manli Wang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 191, P. 108975 - 108975

Published: Aug. 24, 2024

Benzo(a)pyrene (BaP) or benzo (a) pyrene 7,8-dihydrodiol-9,10-epoxide (BPDE) exposure causes trophoblast cell dysfunctions and induces miscarriage, which is generally epigenetically regulated. Homologous recombination (HR) repair of DNA double strand break (DSB) plays a crucial role in maintenance genetic stability normal functions. However, whether BaP/BPDE might suppress HR human cells to induce as well its epigenetic regulatory mechanism, largely unclear. In this study, we find that suppresses DSB eventually miscarriage by up-regulating lnc-HZ08. lnc-HZ08 (1) down-regulates the expression levels FOXA1 (forkhead box A1) thus FOXA1-mediated mRNA transcription BRCA1 (Breast cancer susceptibility gene 1) CtIP (CtBP-interacting protein), (2) impairs protein interactions competitive binding with through lnc-HZ08-1 fragment, also (3) BRCA1-mediated ubiquitination without affecting stability, three cells. Supplement murine Ctip could efficiently restore (i.e. increase) alleviate BaP-exposed mouse model. Collectively, study not only reveals association causality among exposure, defective repair, but discovers novel mechanism lnc-HZ08-regulated BRCA1/CtIP-mediated bridging regulation instability providing an efficient approach for treatment against BaP/BPDE-induced unexplained miscarriage.

Language: Английский

Citations

1
Self-assembled carrier-free formulations based on medicinal and food active ingredients DOI
Hao Yuan,

Haixia Ji,

Li Gao

et al.

Biomaterials Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The popularity of medicinal plants, which have a unique system and are mostly used in compound form for the prevention treatment wide range diseases, is growing worldwide. In recent years, with advances chemical separation structural analysis techniques, many major bioactive molecules plants been identified. However, active ingredients often possess characteristics, including poor water solubility, stability bioavailability, limit their therapeutic applications. To address this problem, self-assembly small from food sources provides new strategy. Driven by various types acting forces, modifiable groups, multiple sites action, hydrophobic side chains, rigid backbones properties able to supramolecular network hydrogels, nanoparticles, micelles, other self-assemblies. This review first summarizes forms self-assemblies such as micelles at level action site, discusses studies on that can be self-assembly, addition summarizing advantages variety disease applications, wound healing, antitumor, anticancer, diabetes mellitus. Finally, problems self-assemblers possible directions future development presented. We firmly believe potential develop effective compounds drug-food homologous providing valuable information drug research strategies perspectives modernization Chinese medicine.

Language: Английский

Citations

1