Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
26, P. 101050 - 101050
Published: April 12, 2024
Periodontal
ligament
(PDL)
cells
play
a
crucial
role
in
maintaining
periodontal
integrity
and
function
by
providing
cell
sources
for
regeneration.
While
biophysical
stimulation
is
known
to
regulate
behaviors
functions,
its
impact
on
epigenetics
of
PDL
has
not
yet
been
elucidated.
Here,
we
aimed
investigate
the
cytoskeletal
changes,
epigenetic
modifications,
lineage
commitment
following
application
stretch
stimuli
PDL.
were
subjected
stretching
(0.1
Hz,
10%).
Subsequently,
changes
focal
adhesion,
tubulin,
histone
modification
observed.
The
survival
ability
inflammatory
conditions
was
also
evaluated.
Furthermore,
using
rat
hypo-occlusion
model,
verified
whether
these
phenomena
are
observed
vivo.
Stretched
showed
maximal
3
acetylation
(H3Ace)
at
2
h,
aligning
perpendicularly
direction.
RNA
sequencing
revealed
altered
gene
sets
related
mechanotransduction,
modification,
reactive
oxygen
species
(ROS)
metabolism,
differentiation.
We
further
found
that
anchorage,
elongation,
actin/microtubule
highly
upregulated
with
mechanosensitive
chromatin
remodelers
such
as
H3Ace
H3
trimethyl
lysine
9
(H3K9me3)
adopting
euchromatin
status.
Inhibitor
studies
mechanotransduction-mediated
alters
behaviors.
displayed
enhanced
against
bacterial
toxin
(C12-HSL)
or
ROS
(H2O2)
attack.
cyclic
priming
osteoclast
osteoblast
differentiation
potential
cells,
evidenced
upregulation
lineage-specific
genes.
In
vivo,
from
normally
loaded
teeth
an
elongated
morphology
higher
levels
compared
hypo-occlusion,
where
mechanical
stimulus
removed.
Overall,
data
strongly
link
external
physical
forces
subsequent
mechanotransduction
impacting
expression
multiple
cellular
behaviors,
important
implications
biology
tissue
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 24, 2025
Alginate
biopolymers
were
modified
with
norbornene
(Nb)
and
tetrazine
(Tz)
functional
groups
to
generate
hydrogel
networks
tunable
ionic
covalent
cross-linking
for
modeling
the
strain-stiffening
behavior
of
extracellular
matrix.
The
mechanical
properties
hydrogels
investigated
by
oscillatory
shear
rheology,
axial
compression,
stress
relaxation
analysis.
Introducing
Nb-Tz
irreversible
cross-links
yielded
dual-cross-linked
stiffer
more
elastic
compared
purely
ionically
cross-linked
alginate
networks.
strain
stiffening
effect
was
observed
under
both
amplitude
sweeps
stepwise
compression
tests
hydrogels.
This
study
provides
valuable
insights
into
structure-property
relationship
biopolymer
designing
matrix
mimics
fibrotic
tissues.
MedComm,
Journal Year:
2025,
Volume and Issue:
6(5)
Published: April 24, 2025
ABSTRACT
Hydrogels
have
emerged
as
dependable
candidates
for
tissue
repair
because
of
their
exceptional
biocompatibility
and
tunable
mechanical
properties.
However,
conventional
hydrogels
are
vulnerable
to
damage
owing
stress
environmental
factors
that
compromise
structural
integrity
reduce
lifespan.
In
contrast,
self‐healing
with
inherent
ability
restore
structure
function
autonomously
offer
prolonged
efficacy
enhanced
appeal.
These
can
be
engineered
into
innovative
forms
including
stimulus‐responsive,
self‐degradable,
injectable,
drug‐loaded
variants,
thereby
enhancing
applicability
in
wound
healing,
drug
delivery,
engineering.
This
review
summarizes
the
categories
mechanisms
hydrogels,
along
biomedical
applications,
repair,
biosensing.
Tissue
includes
bone‐related
nerve
cardiac
repair.
Additionally,
we
explored
challenges
continue
face
presented
a
forward‐looking
perspective
on
development.
Consequently,
it
is
anticipated
will
progressively
designed
developed
applications
extend
beyond
broader
range
applications.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
13(21)
Published: March 27, 2024
Fibrosis,
which
is
primarily
marked
by
excessive
extracellular
matrix
(ECM)
deposition,
a
pathophysiological
process
associated
with
many
disorders,
ultimately
leads
to
organ
dysfunction
and
poor
patient
outcomes.
Despite
the
high
prevalence
of
fibrosis,
currently
there
exist
few
therapeutic
options,
importantly,
paucity
in
vitro
models
accurately
study
fibrosis.
This
review
discusses
multifaceted
nature
fibrosis
from
viewpoint
developing
organ-on-chip
(OoC)
disease
models,
focusing
on
five
key
features:
ECM
component,
inflammation,
mechanical
cues,
hypoxia,
vascularization.
The
potential
OoC
technology
explored
for
better
modeling
these
features
context
studying
fibrotic
diseases
interplay
between
various
emphasized.
paper
reviews
how
organ-specific
are
modeled
platforms,
elements
included
existing
avenues
novel
research
directions
highlighted.
Finally,
this
concludes
perspective
address
current
gap
respect
inclusion
multiple
yield
more
sophisticated
relevant
an
format.
Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
26, P. 101050 - 101050
Published: April 12, 2024
Periodontal
ligament
(PDL)
cells
play
a
crucial
role
in
maintaining
periodontal
integrity
and
function
by
providing
cell
sources
for
regeneration.
While
biophysical
stimulation
is
known
to
regulate
behaviors
functions,
its
impact
on
epigenetics
of
PDL
has
not
yet
been
elucidated.
Here,
we
aimed
investigate
the
cytoskeletal
changes,
epigenetic
modifications,
lineage
commitment
following
application
stretch
stimuli
PDL.
were
subjected
stretching
(0.1
Hz,
10%).
Subsequently,
changes
focal
adhesion,
tubulin,
histone
modification
observed.
The
survival
ability
inflammatory
conditions
was
also
evaluated.
Furthermore,
using
rat
hypo-occlusion
model,
verified
whether
these
phenomena
are
observed
vivo.
Stretched
showed
maximal
3
acetylation
(H3Ace)
at
2
h,
aligning
perpendicularly
direction.
RNA
sequencing
revealed
altered
gene
sets
related
mechanotransduction,
modification,
reactive
oxygen
species
(ROS)
metabolism,
differentiation.
We
further
found
that
anchorage,
elongation,
actin/microtubule
highly
upregulated
with
mechanosensitive
chromatin
remodelers
such
as
H3Ace
H3
trimethyl
lysine
9
(H3K9me3)
adopting
euchromatin
status.
Inhibitor
studies
mechanotransduction-mediated
alters
behaviors.
displayed
enhanced
against
bacterial
toxin
(C12-HSL)
or
ROS
(H2O2)
attack.
cyclic
priming
osteoclast
osteoblast
differentiation
potential
cells,
evidenced
upregulation
lineage-specific
genes.
In
vivo,
from
normally
loaded
teeth
an
elongated
morphology
higher
levels
compared
hypo-occlusion,
where
mechanical
stimulus
removed.
Overall,
data
strongly
link
external
physical
forces
subsequent
mechanotransduction
impacting
expression
multiple
cellular
behaviors,
important
implications
biology
tissue
