Sea Anemone Kunitz Peptide HCIQ2c1 Reduces Histamine-, Lipopolysaccharide-, and Carrageenan-Induced Inflammation via the Suppression of Pro-Inflammatory Mediators

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 431 - 431

Published: Jan. 6, 2025

Inflammation is a physiological response of the immune system to infectious agents or tissue injury, which involves cascade vascular and cellular events activation biochemical pathways depending on type harmful agent stimulus generated. The Kunitz peptide HCIQ2c1 sea anemone Heteractis magnifica strong protease inhibitor exhibits neuroprotective analgesic activities. In this study, we investigated anti-inflammatory potential in histamine- lipopolysaccharide (LPS)-activated RAW 264.7 macrophages as well LPS-induced systemic inflammation carrageenan-induced paw edema models CD-1 mice. We found that 10 μM dramatically decreases histamine-induced intracellular Ca2+ release reactive oxygen species (ROS) production macrophages. Moreover, significantly inhibited tumor necrosis factor α (TNF-α), inducible NO-synthase (iNOS), 5-lipoxygenase (5-LO) but slightly influenced IL-1β cyclooxygenase-2 (COX-2) expression level Furthermore, intravenous administration by at 0.1 mg/kg dose reduced TNF-α, IL-1β, COX-2, iNOS gene subplantar mice two, comparable effect diclofenac 1 dose. Thus, has attenuation local inflammatory effects through inhibition release, ROS pro-inflammatory cytokines, enzymes involved arachidonic acid metabolism.

Language: Английский

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Recent Advances in Cell‐Based In Vitro Models to Recreate Human Intestinal Inflammation

Advanced Science, Journal Year: 2023, Volume and Issue: 10(31)

Published: Sept. 21, 2023

Abstract Inflammatory bowel disease causes a major burden to patients and healthcare systems, raising the need develop effective therapies. Technological advances in cell culture, allied with ethical issues, have propelled vitro models as essential tools study aetiology, its progression, possible Several cell‐based of intestinal inflammation been used, varying their complexity methodology induce inflammation. Immortalized lines are extensively used due long‐term survival, contrast primary cultures that short‐lived but patient‐specific. Recently, organoids organ‐chips demonstrated great potential by being physiologically more relevant. This review aims shed light on intricate nature cover recent works report human inflammation, encompassing diverse approaches outcomes.

Language: Английский

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Pasteurized form of a potential probiotic lactobacillus brevis IBRC-M10790 exerts anti-inflammatory effects on inflammatory bowel disease in vitro

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 10, 2024

Inflammatory bowel disease (IBD) is a chronic, relapsing inflammatory disorder of the gastrointestinal system. So far, no treatment has been identified that can completely cure IBD. Lactobacillus brevis hypothesized to be beneficial in preventing inflammation. This study aimed evaluate potential probiotic effects live and pasteurized L. IBRC-M10790 on vitro cell co-culture model

Language: Английский

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Smart Polymeric 3D Microscaffolds Hosting Spheroids for Neuronal Research via Quantum Metrology

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Toward the aim of reducing animal testing, innovative in vitro models are required. Here, this study proposes a novel smart polymeric microscaffold to establish an advanced 3D model dopaminergic neurons. These scaffolds fabricated with Ormocomp via Two-Photon Polymerization. The further enhanced by functionalization fluorescent nanodiamonds (FNDs), which can serve as quantum nanosensors for both magnetic and temperature sensing. material biocompatibility is tested using two different cell lines, SH-SY5Y A431, viability over 98%. A total 69% FNDs grafted on structure compared those that remained glass surface. Cells several microenvironments, final assembly required metrology experiments achieved 91% biocompatibility. Subsequently, embryoid bodies containing neurons, type affected Parkinson's disease (PD), integrated FND-functionalized scaffolds. This successfully established, demonstrated strong interactions between neurons scaffold, directional growth neurites along scaffold. Ultimately, have developed platform enables readout signaling holds great potential future PD research.

Language: Английский

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Curcumin attenuates ulcerative colitis via regulation of Sphingosine kinases 1/NF‐κB signaling pathway

BioFactors, Journal Year: 2025, Volume and Issue: 51(1)

Published: Jan. 1, 2025

Abstract Curcumin, a compound from Curcuma longa L., has significant anti‐inflammatory properties. However, the mechanisms underlying its activity in dextran sodium sulfate (DSS)‐induced ulcerative colitis (UC) remain inadequately understood. This study aimed to further elucidate molecular of curcumin DSS‐induced UC mice. Our data showed that alleviated by reducing intestinal damage and inflammation, increasing goblet cells colon tissues. Enzyme‐linked immunosorbent assay revealed reduced expression inflammatory cytokines (tumor necrosis factor‐alpha, interleukin‐1β, interleukin‐8) serum myeloperoxidase A comprehensive analysis integrating network pharmacology RNA sequencing (RNA‐seq) enrichment nuclear factor kappa B (NF‐κB) signaling pathways. Notably, RNA‐seq demonstrated significantly downregulated mRNA sphingosine kinase 1 (SphK1). Furthermore, docking can bind SphK1 NF‐κB. Additionally, was found inhibit activation SphK1/NF‐κB pathway tissue. addresses pharmacologic mechanistic perspectives ameliorates response.

Language: Английский

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3D Organoid Culturing Devices to Induce in Vitro Models of Human Intestinal Inflammation

Electronic Materials Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Orally Bioavailable BRD4 BD1 Inhibitor ZL0516 Effectively Suppresses Colonic Inflammation in Animal Models of Inflammatory Bowel Disease

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: 8(4), P. 1152 - 1167

Published: April 1, 2025

Inflammatory bowel disease (IBD), a chronic, progressive, and recurrent gastrointestinal inflammatory disorder, poses significant threat to global health exerts an adverse effect on the quality of life. Currently, there is lack effective therapies for IBD. Developing novel targeted IBD, particularly orally therapeutics, vital need IBD patients. Herein, we first demonstrate that BRD4/NF-κB signaling aberrantly activated in colons human biopsy samples compared normal healthy controls. ZL0516, potent, selective, bioavailable BRD4 BD1 inhibitor, significantly inhibits TNFα- LPS-induced expression cytokines colonic epithelial cells (HCECs) peripheral blood mononuclear (PBMCs) with low cytotoxicity. Intriguingly, when administered preventive mode, ZL0516 blocks dextran sulfate sodium (DSS)-induced murine colitis. When used therapeutic effectively suppresses inflammation several IBD-relevant animal models: DSS-, oxazolone (OXA)-, flagellin (Cbir1) T cell-induced chronic colitis models responses vitro vivo by blocking activation pathway. Also, found RVX208, selective BD2 inhibitor Phase III clinical development, only displayed marginal effects these models. Collectively, our results specific inhibition strategy IBD-associated inflammation, promising candidate development regimen against

Language: Английский

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Per- and Polyfluoroalkyl Substances as Potentiators of Hepatotoxicity in an Exposome Framework: Current Challenges of Environmental Toxicology

Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 154167 - 154167

Published: April 1, 2025

Chronic liver diseases, including metabolic dysfunction-associated steatosic disease (MASLD) and hepatocellular carcinoma (HCC), are on the rise, potentially due to daily exposure complex mixtures of chemical compounds forming part exposome. Understanding mechanisms involved in hepatotoxicity these is essential identify common molecular targets that may highlight potential interactions at level. We illustrated this issue with two families environmental contaminants, per- polyfluoroalkyl substances (PFAS) heterocyclic aromatic amines (HAAs), both which could be progression chronic whose toxicity potentiated by level xenobiotic metabolism. In study exposome effects disease, New Approach Methodologies (NAMs) omics analyses data from various vitro, vivo silico approaches, crucial for improving predictivity toxicological studies humans while reducing animal experimentation. Additionally, development vitro human cell models, such as organoids, avoid interspecies differences minimize risk humans. All together, approaches will contribute construct Adverse Outcome Pathways (AOPs) applied not only PFAS but also other families, providing valuable insights into mixture prediction A better understanding clarify role contaminant MASLD HCC, supporting assessment treatment, monitoring prevention strategies.

Language: Английский

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Squamous cell carcinoma of the small intestine: a case report and review of literature

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: April 30, 2025

Malignant tumors derived from the small intestine are rare, and most adenocarcinomas. Primary squamous cell carcinoma of is sporadic with a few cases reported in literature. This study reports case 56-year-old female who had history leakage exudate for more than 40 years after an appendectomy. The patient presented increasing exudate, abdominal pain, fever this time, was diagnosed primary ileal intestinal resection fascial plasty. declined to receive adjuvant chemotherapeutic treatment died 9 months diagnosis tumor. Additionally, we reviewed 26 cases, summarized clinical features treatments, discussed potential pathogenesis optional therapeutic strategies intestine.

Language: Английский

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Intracellularly Gelated Macrophages Loaded with Probiotics for Therapy of Colitis

Nano Letters, Journal Year: 2024, Volume and Issue: 24(43), P. 13504 - 13512

Published: Oct. 17, 2024

Probiotics therapy has garnered significant attention in the treatment of inflammatory bowel disease (IBD). However, a large number oral administrated probiotics are inactivated after passing through gastric acid environment, and their ability to colonize intestine is also weak. Herein, this study develops novel formulation (GM-EcN) by incorporating Escherichia coli Nissle 1917 (EcN) into intracellularly gelated macrophages (GM). Intracellular hydrogel designed load prevent EcN from digestion juice, GM acts as macrophage-like carrier carry attached intestine. In addition, serves an ideal cytoskeletal structure maintain intact cell morphology membrane GM, comparable source macrophages. Due receptor–ligand interaction, inflammation-related proteins enable sponge sequestrate neutralize multiple cytokines. vivo demonstrates that GM-EcN efficiently alleviates IBD symptoms enhances gut microbiota recovery.

Language: Английский

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