Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
135(33)
Published: June 28, 2023
Abstract
Synthetic
catalytic
DNA
circuits
have
been
recognized
as
a
promising
signal
amplification
toolbox
for
sensitive
intracellular
imaging,
yet
their
selectivity
and
efficiency
are
always
constrained
by
uncontrolled
off‐site
leakage
inefficient
on‐site
circuitry
activation.
Thus,
the
endogenously
controllable
exposure/activation
of
is
highly
desirable
achieving
selective
imaging
live
cells.
Herein,
an
activated
DNAzyme
strategy
was
facilely
integrated
with
circuit
guiding
efficient
microRNA
in
vivo.
To
prevent
activation,
constitute
initially
caged
without
sensing
functions,
which
could
be
selectively
liberated
amplifier
to
guarantee
high‐contrast
target
This
intelligent
modulation
can
tremendously
expand
these
molecularly
engineered
biological
systems.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(33)
Published: June 28, 2023
Synthetic
catalytic
DNA
circuits
have
been
recognized
as
a
promising
signal
amplification
toolbox
for
sensitive
intracellular
imaging,
yet
their
selectivity
and
efficiency
are
always
constrained
by
uncontrolled
off-site
leakage
inefficient
on-site
circuitry
activation.
Thus,
the
endogenously
controllable
exposure/activation
of
is
highly
desirable
achieving
selective
imaging
live
cells.
Herein,
an
activated
DNAzyme
strategy
was
facilely
integrated
with
circuit
guiding
efficient
microRNA
in
vivo.
To
prevent
activation,
constitute
initially
caged
without
sensing
functions,
which
could
be
selectively
liberated
amplifier
to
guarantee
high-contrast
target
This
intelligent
modulation
can
tremendously
expand
these
molecularly
engineered
biological
systems.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 2, 2025
Conventional
solid/liquid
electrochemical
interfaces
typically
encounter
challenges
with
impeded
mass
transport
for
poor
quantification
due
to
the
intricate
pathways
of
reactants
from
bulk
solution.
To
address
this
issue,
work
reports
an
innovative
approach
integrating
a
target-activated
DNA
framework
nanomachine
electrochemically
driven
metal-organic
(MOF)
conversion
self-sacrificial
biosensing.
The
presence
target
biomarker
serotonin
initiates
by
entropy-driven
circuit
form
cross-linked
nanostructure
and
subsequently
release
Fe-MOF
probe.
Acting
as
natural
metal
precursor
nanoconfined
source
reactant,
probe
is
converted
into
electroactive
Prussian
Blue
during
processes.
Taking
advantage
confinement
effect,
our
proposed
biosensor
exhibits
excellent
capability
detect
in
linear
range
1
pM
5
μM
remarkable
detection
limit
0.4
exceptional
specificity
against
other
interferents.
proof-of-concept
demonstration
clinical
serum
samples
patients
carcinoid
tumors
highlights
utility
complex
sample
analysis.
design
could
be
applied
high
potential
inspire
sensing
approaches,
holding
promise
applications
biomedical
research
disease
diagnosis.
Advanced Science,
Journal Year:
2023,
Volume and Issue:
10(19)
Published: April 21, 2023
MicroRNAs
(miRNAs)
can
act
as
oncogenes
or
tumor
suppressors,
capable
of
up
down-regulating
gene
expression
during
tumorigenesis;
they
are
diagnostic
biomarkers
therapeutic
targets
for
tumors.
To
detect
low
abundance
intracellular
oncogenic
miRNAs
(onco-miRNAs)
and
realize
synergistic
therapy
onco-miRNAs
a
smart
nano-theranostic
platform
based
on
dual-miRNAs
guided
self-feedback
tetrahedral
entropy-driven
DNA
circuit
is
created.
The
delivery
vehicle
framework,
in
which
the
achieves
self-feedback,
between
an
situ
amplification
activation
suppressor
release.
test
this
platform,
selected,
miRNA-155,
up-regulated
miRNA,
cancer
indicators,
miRNA-122,
down-regulated
miRNA
hepatocellular
carcinoma,
respectively.
Through
circuit,
to
at
femtomolar
level
well
visualized
inside
cells,
fixed
tissues,
mice
programmed.
Furthermore,
triggered
by
preloaded
miRNA-122
amplified
via
released
into
target
cells;
sudden
increase
simultaneous
decrease
miRNA-155
synergistically
served
drugs
regulation
with
enhanced
antitumor
efficacy
superior
biosafety.
It
envisioned
that
will
initiate
essential
step
toward
theranostics
personalized/precise
medicine.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(22)
Published: April 13, 2024
Abstract
The
precise
regulation
of
cellular
behaviors
within
a
confined,
crowded
intracellular
environment
is
highly
amenable
in
diagnostics
and
therapeutics.
While
synthetic
circuitry
system
through
concatenated
chemical
reaction
network
has
rarely
been
reported
to
mimic
dynamic
self‐assembly
system.
Herein,
catalytic
self‐defined
circuit
(CSC)
for
the
hierarchically
assembly
DNA
domino
nanostructures
engineered.
By
incorporating
pre‐sealed
symmetrical
fragments
into
preying
hairpin
reactants,
CSC
allows
hierarchical
via
microRNA
(miRNA)‐powered
self‐sorting
hybridization
reaction.
With
minimal
strand
complexity,
this
self‐sustainable
streamlined
component
achieved
localization‐intensified
cascaded
signal
amplification.
Profiting
from
self‐adaptively
reaction,
reliable
robust
method
discriminating
carcinoma
tissues
corresponding
para‐carcinoma
tissues.
CSC‐sustained
strategy
provides
comprehensive
smart
toolbox
organizing
various
nanostructures,
which
may
facilitate
more
insights
clinical
diagnosis
therapeutic
assessment.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(14), P. 5560 - 5569
Published: March 26, 2024
Catalytic
DNA
circuits
are
desirable
for
sensitive
bioimaging
in
living
cells;
yet,
it
remains
a
challenge
to
monitor
these
intricate
signal
communications
because
of
the
uncontrolled
circuitry
leakage
and
insufficient
cell
selectivity.
Herein,
simple
yet
powerful
DNA-repairing
enzyme
(APE1)
activation
strategy
is
introduced
achieve
site-specific
exposure
catalytic
circuit
realizing
selectively
amplified
imaging
intracellular
microRNA
robust
evaluation
APE1-involved
drug
resistance.
Specifically,
reactants
firmly
blocked
by
recognition/cleavage
site
prevent
undesirable
off-site
leakage.
The
caged
has
no
target-sensing
activity
until
its
components
activated
via
enzyme-mediated
structural
reconstitution
finally
transduces
fluorescence
within
miRNA
stimulation.
designed
demonstrates
an
enhanced
signal-to-background
ratio
assay
as
compared
with
conventional
enables
cancer-cell-selective
miRNA.
In
addition,
shows
sensing
performance
visualizing
APE1-mediated
chemoresistance
cells,
which
anticipated
in-depth
clinical
diagnosis
chemotherapy
research.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(32)
Published: June 25, 2024
Abstract
Multidrug
resistance
(MDR)
is
a
major
obstacle
limiting
the
effectiveness
of
chemotherapy
against
cancer.
The
combination
strategy
chemotherapeutic
agents
and
siRNA
targeting
drug
efflux
has
emerged
as
an
effective
cancer
treatment
to
overcome
MDR.
Herein,
stimuli‐responsive
programmable
tetrahedral
DNA‐RNA
nanocages
(TDRN)
have
been
rationally
designed
developed
for
dynamic
co‐delivery
doxorubicin
P‐glycoprotein
(P‐gp)
siRNA.
Specifically,
sense
antisense
strand
sequences
P‐gp
siRNA,
which
are
bricks
with
terminal
disulfide
bond
conjugation,
precisely
embedded
in
one
edge
DNA
tetrahedron.
TDRN
provides
release
element
control
functional
cargo
that
significantly
more
stable
than
“tail‐like”
TDN
nanostructures.
highly
rigid
3D
nanostructure
siRNA‐organized
demonstrated
notable
improvement
stability
RNase
A
mouse
serum,
well
long‐term
storage
up
4
weeks,
evidenced
by
this
study.
These
biocompatible
multifunctional
nanocarriers
gold
nanocluster‐assisted
delivery
(TDRN@Dox@AuNC
p
)
successfully
used
achieve
synergistic
RNAi/Chemo‐therapy
vitro
vivo.
This
system,
integrates
RNAi
therapy
chemotherapy,
offers
promising
approach
treating
multidrug‐resistant
tumors.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
An
entropy-driven
catalysis
(EDC)
strategy
is
appealing
for
amplified
bioimaging
of
microRNAs
in
living
cells;
yet,
complex
operation
procedures,
lacking
cell
selectivity,
and
insufficient
accuracy
hamper
its
further
applications.
Here,
we
introduce
an
ingenious
all-in-one
DNA
nanomachine
(termed
as
AIO-EDN),
which
can
be
triggered
by
endogenous
apurinic/apyrimidinic
endonuclease
1
(APE1)
to
achieve
tumor
cell-selective
dual-mode
imaging
microRNA.
Compared
with
the
traditional
EDC
strategy,
integrated
design
AIO-EDN
achieves
autocatalytic
signal
amplification
without
extra
fuel
strands.
Moreover,
leverages
APE1
overexpressed
cancer
cells
activate
reaction,
which,
however,
exerts
no
target
sensing
activity
normal
cells.
Combining
fluorescence-
surface-enhanced
Raman
scattering
(FL/SERS)
techniques,
this
exhibits
significantly
improved
selectivity
microRNA
This
study
provides
a
new
paradigm
develop
EDC-based
platform
shows
great
potential
in-depth
diagnosis
high
precision.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 5, 2025
Heart
failure
(HF)
is
a
globally
threatening
cardiovascular
disease
associated
with
poor
quality
of
life
and
high
mortality,
therefore,
timely
diagnosis
risk
prediction
for
HF
are
urgently
needed.
Herein,
compact
yet
robust
self-regenerated
hybridization
circuit
(SHC)
aptasensor
developed
the
amplified
detection
N-terminal
pro-brain
natriuretic
peptide
(NT-proBNP),
"gold
standard
biomarker"
HF.
The
aptamer
transduction
module
can
specifically
recognize
NT-proBNP,
thus
initiating
cascade
reaction
successively
triggers
that
reversely
initiate
cross-hybridization
reaction.
Benefiting
from
aptamer-specific
recognition
self-replicated
signal
amplification,
SHC
demonstrated
more
impressive
diagnostic
performance
in
elderly
patients
than
clinical
fluorescence
immunochromatography
assay
(FICA)
terms
positive
predictive
value
(21
vs
17),
specificity
(39
32),
accuracy
(37
36).
Furthermore,
this
approach
allows
differentiation
among
varying
severities,
achieving
sufficiently
78.3%,
facilitating
accurate
therapeutic
intervention.
versatile
reliable
system
offers
new
to
analyzing
low-abundance
biomarkers
rare
specimens,
which
highly
important
early
prognosis
assessment.
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(8), P. 4991 - 5007
Published: Aug. 1, 2024
The
GPS-Nanoconveyor
(MA-NV@DOX-Cas13a)
is
a
targeted
nanoplatform
designed
for
the
imaging
and
gene/chemotherapy
synergistic
treatment
of
melanoma.
It
utilizes
rolling
circle
amplification
(RCA)
products
as
scaffold
to
construct
DNA
"Nanoconveyor"
(NV),
which
incorporates
multivalent
aptamer
(MA)
"GPS",
encapsulates
doxorubicin
(DOX)
in
transporter,
equips
it
with
CRISPR/Cas13a
ribonucleoproteins
(Cas13a
RNP).
Carrying
MA
enhances
ability
recognize
overexpressed
receptor
nucleolin
on
B16
cells,
enabling
precise
delivery
MA-NV@DOX-Cas13a
through
receptor-mediated
endocytosis.
activation
signal
transducer
activator
transcription
3
(STAT3)
cancer
cells
triggers
cis-cleavage
CRISPR/Cas13a,
initiating
its
trans-cleavage
function.
Additionally,
deoxyribonuclease
I
(DNase
I)
degrades
MA-NV,
releasing
DOX
intracellular
chemotherapeutic
agent.
Experiments
demonstrate
superior
capabilities
this
versatile
cellular
co-treatment
while
highlighting
advantages
these
nanodrug
systems
mitigating
side
effects.
