Plant virology in the 21st century in China: Recent advances and future directions

Journal of Integrative Plant Biology, Journal Year: 2023, Volume and Issue: 66(3), P. 579 - 622

Published: Nov. 4, 2023

Plant viruses are a group of intracellular pathogens that persistently threaten global food security. Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years, including basic research and technologies for preventing controlling viral diseases. Here, we review these milestones advances, identification new crop-infecting viruses, dissection pathogenic mechanisms multiple examination multilayered interactions among their host plants, virus-transmitting arthropod vectors, in-depth interrogation plant-encoded resistance susceptibility determinants. Notably, various virus-based vectors also successfully developed gene function studies target expression plants. We recommend future China.

Language: Английский

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A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB

Advanced Science, Journal Year: 2023, Volume and Issue: 10(29)

Published: Aug. 16, 2023

Abstract Mycovirus‐mediated hypovirulence has the potential to control fungal diseases. However, availability of hypovirulence‐conferring mycoviruses for plant disease is limited as most viruses are asymptomatic. In this study, virus‐induced gene silencing (VIGS) vector p26‐D4 Fusarium graminearum gemytripvirus 1 (FgGMTV1), a tripartite circular single‐stranded DNA mycovirus, successfully constructed convert causal fungus cereal head blight (FHB) into hypovirulent strain. p26‐D4, with an insert 75–150 bp fragment target reporter transgene transcript in both sense and antisense orientations, efficiently triggered . Notably, two strains, p26‐D4‐Tri101, p26‐D4‐FgPP1, obtained by virulence‐related genes Tri101 FgPP1 can be used biocontrol agents protect wheat from FHB mycotoxin contamination at field level. This study not only describes first mycovirus‐derived VIGS system but also proves that establish multiple strains pathogenic fungi.

Language: Английский

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A circular single-stranded DNA mycovirus infects plants and confers broad-spectrum fungal resistance

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(6), P. 955 - 971

Published: May 13, 2024

Circular single-stranded DNA (ssDNA) viruses have been rarely found in fungi, and the evolutionary ecological relationships among ssDNA infecting fungi other organisms remain unclear. In this study, a novel circular virus, tentatively named Diaporthe sojae virus 1 (DsCDV1), was identified phytopathogenic fungus isolated from pear trees. DsCDV1 has monopartite genome (3185 nt size) encapsidated isometric virions (21-26 nm diameter). The comprises seven putative open reading frames encoding discrete replicase (Rep) split by an intergenic region, capsid protein (CP), several proteins of unknown function (P1-P4), long region. Notably, two parts Rep share high identities with Reps Geminiviridae Genomoviridae, respectively, indicating linkage both families. Phylogenetic analysis based on or CP sequences placed unique cluster, supporting establishment new family, Gegemycoviridae, intermediate to significantly attenuates fungal growth nearly erases virulence when transfected into host fungus. Remarkably, can systematically infect tobacco seedlings, providing broad-spectrum resistance diseases. Subcellular localization revealed that P3 is localized plasmodesmata, while its expression trans-complementation experiments could restore systematic infection movement-deficient plant suggesting movement protein. exhibits molecular biological traits not observed viruses, serving as link between presenting connection fungi. These findings contribute expanding our understanding diversity evolution, offering potential biocontrol applications for managing crucial

Language: Английский

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Greetings from virologists to mycologists: A review outlining viruses that live in fungi

Mycoscience, Journal Year: 2024, Volume and Issue: 65(1), P. 1 - 11

Published: Jan. 30, 2024

Viruses are genetic elements that parasitize self-replicating cells. Therefore, organisms parasitized by viruses not limited to animals and plants but also include microorganisms. Among these, fungi known as mycoviruses. Mycoviruses with an RNA genome persistently replicate inside fungal cells coevolve their host cells, similar a cellular organelle. Within mycoviruses can modulate various characteristics activities, including pathogenicity the production of enzymes secondary metabolites. In this review, we provide overview mycovirus research field introduction researchers. Recognition all in aids towards better understanding control fungi, makes significant model system for studying microorganisms containing multiple elements.

Language: Английский

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Advances in Understanding Fusarium graminearum: Genes Involved in the Regulation of Sexual Development, Pathogenesis, and Deoxynivalenol Biosynthesis

Genes, Journal Year: 2024, Volume and Issue: 15(4), P. 475 - 475

Published: April 9, 2024

The wheat head blight disease caused by Fusarium graminearum is a major concern for food security and the health of both humans animals. As pathogenic microorganism, F. produces virulence factors during infection to increase pathogenicity, including various macromolecular small molecular compounds. Among these factors, secreted proteins deoxynivalenol (DON) are important weapons expansion colonization graminearum. Besides presence sexual reproduction also crucial process indispensable emergence spread blight. Over last ten years, there have been notable breakthroughs in researching This review aims analyze research progress reproduction, proteins, DON graminearum, emphasizing regulation synthesis. We discuss application new gene engineering technologies prevention control

Language: Английский

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Harnessing RNA interference for the control of Fusarium species: A critical review

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(10)

Published: Oct. 1, 2024

Fusarium fungi are a pervasive threat to global agricultural productivity. They cause spectrum of plant diseases that result in significant yield losses and threaten food safety by producing mycotoxins harmful human animal health. In recent years, the exploitation RNA interference (RNAi) mechanism has emerged as promising avenue for control Fusarium-induced diseases, providing both mechanistic understanding gene function potential strategy environmentally sustainable disease management. However, despite progress elucidating presence RNAi pathway different species, comprehensive its individual protein components underlying silencing mechanisms remains elusive. Accordingly, while considerable number RNAi-based approaches have been developed many reports applications under laboratory conditions published, applicability this knowledge agronomic settings an open question, few convincing data on field published. This review aims consolidate current role evaluating research highlighting important avenues future investigation.

Language: Английский

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Spray-induced gene silencing for crop protection: recent advances and emerging trends

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 20, 2025

The RNA-based spray-induced gene silencing (SIGS) technology represents an ecologically sustainable approach to crop protection and pathogen management. Following the recent approval of Ledprona as first sprayable double-stranded RNA (dsRNA) biopesticide by EPA at end 2023, SIGS has emerged a focal point in both academic industrial sectors. This review analyzes advances emerging trends SIGS. application for protection, including control insects, fungal pathogens, viruses, is briefly summarized. Distinguishing this from others, we delve into practical aspects technology, such selection screening target genes, large-scale production methods, delivery systems, highlighting major advancements these areas also addressing remaining questions issues, particularly concerning safety concerns controlling harmful weeds. Finally, emphasizes its integration with nanotechnology other methodologies. Collectively, rapid progress studies poised accelerate maturation technology.

Language: Английский

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Discovery of novel mycoviruses from fungi associated with mango leaf spots

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Mango (Mangifera indica) is a commercially significant fruit crop cultivated globally. However, leaf spot diseases are common in mango orchards, which severely impact the yield. Mycoviruses hold promise as potential biocontrol agents. To investigate this possibility, fungi were isolated from lesions, resulting identification of six strains that contained double-stranded RNA (dsRNA). Through BLASTx analysis NCBI non-redundant database, 27 mycovirus-related contigs identified, corresponded to 10 distinct viruses grouped into 8 lineages: Alternaviridae, Chrysoviridae, Partitiviridae, Polymycoviridae, Orthototiviridae, Deltaflexiviridae, Narnaviridae, and Bunyaviricetes. Full genomic sequences these characterized confirmed be associated with their host fungi. The findings included novel mycoviruses, three previously unreported discovered new hosts, one virus strain. These results highlight diversity taxonomy mycoviruses found spots.

Language: Английский

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Nanotechnology-driven gene silencing: advancements in SIGS–dsRNA technology for sustainable disease management

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 4, 2025

Among the fungal diseases, Fusarium head blight (FHB), caused by graminearum, is one of most destructive disease affecting wheat. This pathogen poses significant threats to global wheat production, leading substantial yield losses and contaminating grains with harmful mycotoxins. The chemical control FHB has become increasingly challenging due rise resistance, environmental concerns, effects climate change. review introduces a novel approach management through spray-induced gene silencing (SIGS), cutting-edge technology that uses double-stranded RNA (dsRNA) silence critical genes in both fungus host plant. reduces virulence enhances plant resilience. A key innovation integration nanotechnology improve delivery dsRNA, addressing challenges related stability, cellular uptake, targeting efficiency field conditions. Nanocarriers have revolutionized dsRNA improving its encapsulation efficiency, precision, compared traditional methods. Advances cost-effective particularly microbial expression systems, enable scalable sustainable implementation this technology. emphasizes potential nanocarrier systems precision agriculture highlights their role replacing treatments interference (RNAi)-based solutions. RNAi-based approaches not only reduce reliance on synthetic chemicals, but also promote sustainability fungicide resistance. However, remain large-scale application, cost-effectiveness, regulatory approval processes. Overcoming these hurdles will be crucial unlocking full In conclusion, combination SIGS-based offers groundbreaking managing infections innovative strategy minimize impacts while enhancing food security, paving way for more resilient agricultural future.

Language: Английский

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Microbiome engineering to palliate microbial dysbiosis occurring in agroecosystems

Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128178 - 128178

Published: April 1, 2025

Language: Английский

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