The gut microbiota-produced vitamin B6 mitigates alcohol-associated liver disease by attenuating hepatic oxidative stress damage DOI Creative Commons
Haiyuan Shen, Liangliang Zhou,

Yuanru Yang

et al.

Hepatology Communications, Journal Year: 2024, Volume and Issue: 9(1)

Published: Dec. 11, 2024

Background: Alcohol-associated liver disease (ALD) is a major clinical issue characterized by progressive stages, including hepatic steatosis, fibrosis, cirrhosis, and HCC. Patients with long-term chronic alcoholism often present gut microbiota dysbiosis reduced plasma levels of vitamin B 6 . This study aimed to verify that disruption in ALD significantly contributes vivo production investigate the role this reduction pathogenesis ALD. Methods: The was investigated utilizing Gao-binge mouse model. Fecal microbial composition analyzed pair-fed mice identify alcohol-induced functional changes microbiota. Additionally, protein expression profiles metabolomic were elucidate through integrated proteomic analyses. findings further validated using animal models patient samples. Results: Alcohol consumption disrupted mice, impairing synthesis intestinal microorganisms. Vitamin deficiency aggravated disorder amino acid metabolism inhibited ornithine aminotransferase expression, thereby worsening oxidative stress damage. In patients ALD, significant disturbances observed, along decreased levels, which negatively correlated serum biochemical markers. Conclusions: imbalance reduces synthesis, affects glutathione liver, exacerbating These suggest may play critical protective progression regulating metabolism.

Language: Английский

Chlorogenic Acid: A Systematic Review on the Biological Functions, Mechanistic Actions, and Therapeutic Potentials DOI Open Access
Vi Nguyen,

Elaine G. Taine,

Dehao Meng

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(7), P. 924 - 924

Published: March 23, 2024

Chlorogenic acid (CGA) is a type of polyphenol compound found in rich concentrations many plants such as green coffee beans. As an active natural substance, CGA exerts diverse therapeutic effects response to variety pathological challenges, particularly conditions associated with chronic metabolic diseases and age-related disorders. It shows multidimensional functions, including neuroprotection for neurodegenerative disorders diabetic peripheral neuropathy, anti-inflammation, anti-oxidation, anti-pathogens, mitigation cardiovascular disorders, skin diseases, diabetes mellitus, liver kidney injuries, anti-tumor activities. Mechanistically, its integrative functions act through the modulation anti-inflammation/oxidation homeostasis. can thwart inflammatory constituents at multiple levels curtailing NF-kB pathways neutralize primitive factors, hindering propagation, alleviating inflammation-related tissue injury. concurrently raises pivotal antioxidants by activating Nrf2 pathway, thus scavenging excessive cellular free radicals. elevates AMPK maintenance restoration homeostasis glucose lipids. Additionally, neuromodulation targeting neuroreceptors ion channels. In this review, we systematically recapitulate CGA’s pharmacological activities, medicinal properties, mechanistic actions potential agent. Further studies defining specific molecules, improving bioavailability, validating clinical efficacy are required corroborate CGA.

Language: Английский

Citations

68
Colon‐Targeted Ginseng Polysaccharides‐Based Microspheres for Improving Ulcerative Colitis via Anti‐Inflammation and Gut Microbiota Modulation DOI Open Access
Dongxue Huo,

Yanfei Li,

Lijun Song

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Natural plant‐derived polysaccharides exhibit substantial potential for treating ulcerative colitis (UC) owing to their anti‐inflammatory and antioxidant properties favorable safety profiles. However, practical application faces several challenges, including structural instability in gastric acid, imprecise targeting of inflamed regions, limited intestinal retention times. To address these limitations, pH‐responsive, colon‐targeting microspheres (pWGPAC MSs) are developed delivering phosphorylated wild ginseng (pWGP) alleviate UC. These pWGPAC MSs fabricated by incorporating pWGP into calcium‐crosslinked alginate with subsequent chitosan surface modification enhance mucosal adhesion. demonstrated exceptional stability under acidic conditions while enabling targeted release the colon. In a mouse model UC, effectively mitigated injury, attenuated inflammation, restored barrier function. Further mechanistic investigations revealed that modulated TLR4/MYD88 signaling pathway promoted M2 macrophage polarization. Integrated microbiome metabolome analyses regulated gut microbiota composition decreased pro‐inflammatory metabolite levels. addition, promising Collectively, findings establish as therapeutic strategy treatment UC provide solid foundation future clinical applications.

Language: Английский

Citations

2
Chlorogenic Acid Ameliorates Post‐Infectious Irritable Bowel Syndrome by Regulating Extracellular Vesicles of Gut Microbes DOI Creative Commons
Cihua Zheng,

Yuchun Zhong,

Wenming Zhang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(28)

Published: Aug. 24, 2023

Post-infectious irritable bowel syndrome (PI-IBS) occurs after acute infectious diarrhea, and dysbiosis can be involved in its pathogenesis. Here, the role of chlorogenic acid (CGA) is investigated, a natural compound with several pharmacological properties, alleviating PI-IBS rats. It elucidated that gut microbiota plays key pathogenesis rectal administration CGA alleviated by modulating metabolites. supplementation significantly increased fecal Bacteroides acidifaciens abundance glycine levels. Glycine structurally altered B. extracellular vesicles (EVs) enriched functional proteins EVs; glycine-induced EVs reducing inflammation hypersensitivity intestinal viscera maintaining mucosal barrier function. Moreover, are brain tissue. Thus, mediates mitigation through This study proposes novel mechanism signal exchange between microenvironment host.

Language: Английский

Citations

26
Bacteroides acidifaciens and its derived extracellular vesicles improve DSS-induced colitis DOI Creative Commons
Cihua Zheng,

Yuchun Zhong,

Jian Xie

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 30, 2023

"Probiotic therapy" to regulate gut microbiota and intervene in intestinal diseases such as inflammatory bowel disease (IBD) has become a research hotspot. Bacteroides acidifaciens, new generation of probiotics, shown beneficial effects on various diseases.In this study, we utilized mouse colitis model induced by dextran sodium sulfate (DSS) investigate how B. acidifaciens positively affects IBD. We evaluated the ofB. fecal transplantation, bacterial extracellular vesicles (EVs) DSS-induced mice. monitored phenotype colitis, detected serum factors using ELISA, mucosal barrier function Western blotting tissue staining, 16S rRNA sequencing, analyzed differences EVs protein composition derived from proteomics explore positive impact colitis.We confirmed that protective effect including alleviating phenotype, reducing response, improving function, accompanied an increase relative abundance Ruminococcus callidus but decrease fragilis. Further transplantation or filtrate eosinophil-regulated metabolites colitis. Finally, validated contain rich functional proteins can contribute relief colitis.Therefore, its alleviate damage colon tissue, promoting repair, restoring diversity, balance The results study provide theoretical basis for preclinical application probiotics.

Language: Английский

Citations

26
Target fishing and mechanistic insights of the natural anticancer drug candidate chlorogenic acid DOI Creative Commons
Qinghua Wang, Tingting Du, Zhi‐Hui Zhang

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2024, Volume and Issue: 14(10), P. 4431 - 4442

Published: July 6, 2024

Chlorogenic acid (CGA) is a natural product that effectively inhibits tumor growth, demonstrated in many preclinical models, and phase II clinical trials for patients with glioma. However, its direct proteomic targets anticancer molecular mechanisms remain unknown. Herein, we developed novel bi-functional photo-affinity probe PAL/CGA discovered mitochondrial acetyl-CoA acetyltransferase 1 (ACAT1) was one of the main target proteins CGA by using affinity-based protein profiling (AfBPP) chemical approach. We performed in-depth studies on ACAT1/CGA interactions

Language: Английский

Citations

8
Polyphenols improve non‐alcoholic fatty liver disease via gut microbiota: A comprehensive review DOI Creative Commons
Kimia Mohammadhasani, Mohammad Vahedi Fard, Ali Mottaghi Moghaddam Shahri

et al.

Food Science & Nutrition, Journal Year: 2024, Volume and Issue: 12(8), P. 5341 - 5356

Published: June 7, 2024

Abstract Polyphenols, natural micronutrients derived from plants, are valued for their anti‐inflammatory and antioxidant properties. The escalating global prevalence of non‐alcoholic fatty liver disease (NAFLD) underscores its status as a chronic progressive condition. Furthermore, the dysregulation gut microbiota (GM) is implicated in onset progression NAFLD through actions metabolites such bile acids (BAs), lipopolysaccharide (LPS), choline, short‐chain (SCFAs). Additionally, GM may influence integrity intestinal barrier. This review aims to evaluate potential effects polyphenols on barrier function, subsequent impact NAFLD. We searched wide range databases, Web Science, PubMed, EMBASE, Scopus gather information our non‐systematic English literature. functions composition can be regulated by chlorogenic acid, curcumin, green tea catechins, naringenin, quercetin, resveratrol, sulforaphane. Regulating improves alleviating inflammation, fat accumulation, enzymes. it serum lipid profile integrity. All these components affect GM, including SCFAs, LPS, BAs. Current evidence indicates that curcumin modulate improving positively impacting More studies necessary safety efficacy sulforaphane, catechin.

Language: Английский

Citations

6
Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis DOI Creative Commons
Wenjun Zhang, Jian Xie,

Zhuoya Wang

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128047 - 128047

Published: Jan. 9, 2025

Male osteoporosis is primarily caused by a decrease in testicular testosterone production. remains disease with insufficient diagnosis and treatment, its consequences are severe, especially older patients. The gut microbiota plays crucial role occurrence development. Our study found that the relative abundance of Lactobacillus salivarius fecal male patients was significantly lower than healthy volunteers. Animal experiments have shown orchiectomy (ORX) can induce disrupt intestinal mucosal barrier, microbiota. In addition, we discovered potential etiological connection between decreased bacterium L. ORX-induced osteoporosis. Cohousing or direct colonization from rats oral administration bacteria alleviated repaired barrier. Finally, demonstrated extracellular vesicles (EVs) could be transported to bones mitigate rats. results indicate participates protecting secreting delivering bacterial EVs, reduction EVs closely related development androgen deficiency-related

Language: Английский

Citations

0
Platinum micromotors for mucosal penetration and colorectal cancer therapy DOI
Qiang Yang,

Ya Ma,

Menghang Zu

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 670, P. 125201 - 125201

Published: Jan. 10, 2025

Language: Английский

Citations

0
ScRNA-seq unveils the functional characteristics of glioma-associated macrophages and the regulatory effects of chlorogenic acid on the immune microenvironment—a study based on mouse models and clinical practice DOI Creative Commons
Jiachen Wang, Shenglan Li, Yuxiao Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Glioma is the most common primary malignant brain tumor. Despite advances in surgical techniques and treatment regimens, therapeutic effects of glioma remain unsatisfactory. Immunotherapy has brought new hope to patients, but its outcomes are limited by immunosuppressive nature tumor microenvironment (TME). This study aimed reveal subpopulations functional characteristics tumor-associated macrophages (TAMs) explore regulatory chlorogenic acid (CHA) on immune microenvironment, as well potential for clinical application. In this study, CHA was used model mice. ScRNA - seq analysis conducted elucidate differentiation trajectories bone marrow derived monomacrophages (BMDMs) microglia. A PPI molecular docking were constructed using target prediction database. case a patient treated with reviewed. slowed growth mice extended survival time It enhanced antigen presenting function T cell activation related gene expression, activated microglia through JAK STAT pathway, improved antitumor functions. The good affinity STAT1 confirmed. survived 5 years 6 months, achieved partial remission (PR) after 9 months treatment, remained alive without any symptoms or toxic side effects. Our revealed subtypes TAMs. significantly modulating BMDMs may provide insights into targeting regulation TME offer theoretical practical support application CHA. results demonstrated improving effects, which could have implications future strategies.

Language: Английский

Citations

0
Lactobacillus vaginalis alleviates DSS induced colitis by regulating the gut microbiota and increasing the production of 3-indoleacrylic acid DOI Creative Commons

Zhuoya Wang,

Liu Tian, Li Liu

et al.

Pharmacological Research, Journal Year: 2025, Volume and Issue: 213, P. 107663 - 107663

Published: Feb. 16, 2025

Language: Английский

Citations

0