Highly Customizable 3D Microelectrode Arrays for In Vitro and In Vivo Neuronal Tissue Recordings

Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)

Published: Jan. 19, 2024

Abstract Planar microelectrode arrays (MEAs) for – in vitro or vivo neuronal signal recordings lack the spatial resolution and sufficient signal‐to‐noise ratio (SNR) required a detailed understanding of neural network function synaptic plasticity. To overcome these limitations, highly customizable three‐dimensional (3D) printing process is used combination with thin film technology self‐aligned template‐assisted electrochemical deposition to fabricate 3D‐printed‐based MEAs on stiff flexible substrates. Devices design flexibility physical robustness are shown recording activity different applications, achieving high‐aspect 3D microelectrodes up 33:1. Here, successfully record cultures, retinal explants, cortex living mice, thereby demonstrating versatility MEA while maintaining high‐quality recordings. Customizable provide unique opportunities study under regular various pathological conditions, both vivo, contribute development drug screening neuromodulation systems that can accurately monitor large networks over time.

Language: Английский

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Recent progress of soft and bioactive materials in flexible bioelectronics

Cyborg and Bionic Systems, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

Materials that establish functional, stable interfaces to targeted tissues for long-term monitoring/stimulation equipped with diagnostic/therapeutic capabilities represent breakthroughs in biomedical research and clinical medicine. A fundamental challenge is the mechanical chemical mismatch between implants ultimately results device failure corrosion by biofluids associated foreign body response. Of particular interest development of bioactive materials at level chemistry mechanics high-performance, minimally invasive function, simultaneously tissue-like compliance vivo biocompatibility. This review summarizes most recent progress these purposes, an emphasis on material properties such as response, integration schemes biological tissues, their use bioelectronic platforms. The article begins overview emerging classes platforms bio-integration proven utility live animal models, high performance different form factors. Subsequent sections various flexible, soft materials, ranging from self-healing hydrogel/elastomer bio-adhesive composites materials. Additional discussions highlight examples active systems support electrophysiological mapping, stimulation, drug delivery treatments related diseases, spatiotemporal resolutions span cellular organ-scale dimension. Envisioned applications involve advanced brain, cardiac, other organ systems, offer stability human subjects models. Results will inspire continuing advancements functions benign thus yielding therapy diagnostics healthcare.

Language: Английский

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Harnessing Intelligence from Brain Cells In Vitro

The Neuroscientist, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Harnessing intelligence from brain cells in vitro requires a multidisciplinary approach integrating wetware, hardware, and software. Wetware comprises the themselves, where differentiation induced pluripotent stem offers ethical scalability; hardware typically involves life support system setup to record activity deliver stimulation cells; software is required control process signals coming going cells. This review provides broad summary of foundational technologies underpinning these components, along with outlining importance technology integration. Of particular that this new ability extend beyond traditional methods assess primarily survival spontaneous neural cultures. Instead, focus returns core function tissue: neurocomputational information respond accordingly. Therefore, also covers work that, despite relatively early state current technology, has provided novel meaningful understandings field neuroscience opening exciting avenues for future research.

Language: Английский

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Flexible and stretchable bioelectronics for organoids

Med-X, Journal Year: 2025, Volume and Issue: 3(1)

Published: Feb. 1, 2025

Abstract Organoids have gained significant interest due to their ability recapitulate the structural, molecular, and functional complexity of corresponding organs. While methods been developed characterize benchmark organoid structural molecular properties, capturing development maturation organoids remains challenging. To address this, multifunctional bioelectronics for interfacing with has actively pursued. However, conventional electronics face limitations in achieving recording control across entire three-dimensional (3D) volume a long-term stable manner large morphological cellular composition changes during development. In this review, we first discuss application interfacing. We then focus on flexible stretchable designed create organoid/electronics hybrids chronically interfaces. also review recent advancements charting multimodal cell activities throughout Furthermore, explore integration other characterization modalities comprehensive cells within 3D tissues. Finally, potential integrating artificial intelligence into system through embedded electronics, harnessing biosymbiotic computational systems. These could provide valuable tools characterizing maturation, establishing patient-specific models, developing therapeutic opportunities, exploring novel strategies. Graphical abstract

Language: Английский

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Cellular level cryo-neuromodulation using rapid and localized cooling device combined with microelectrode array

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 277, P. 117257 - 117257

Published: Feb. 13, 2025

Language: Английский

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Fabrication of individually addressable microtip electrode array on a flexible substrate

Sensors and Actuators A Physical, Journal Year: 2025, Volume and Issue: unknown, P. 116412 - 116412

Published: March 1, 2025

Language: Английский

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Flexible 3D Kirigami Probes for In Vitro and In Vivo Neural Applications

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Abstract 3D microelectrode arrays (MEAs) are gaining popularity as brain–machine interfaces and platforms for studying electrophysiological activity. Interactions with neural tissue depend on the electrochemical, mechanical, spatial features of recording platform. While planar or protruding 2D MEAs limited in their ability to capture activity across layers, existing still require advancements manufacturing scalability, resolution, integration. In this work, a customizable, scalable, straightforward approach fabricate flexible kirigami containing both surface penetrating electrodes, designed interact space tissue, is presented. These novel probes feature up 512 electrodes distributed 128 shanks single device, shank heights reaching 1 mm. The successfully deployed several applications, vitro vivo, identified spatially dependent patterns. Flexible therefore powerful tool large‐scale electrical sampling complex tissues while improving integration offering enhanced capabilities analyzing disorders disease models where high resolution required.

Language: Английский

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High‐Density Flexible Neural Implants with Submicron Feedline Resolution

Advanced Electronic Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract The development of high‐density microelectrode arrays (MEAs) for large‐scale brain recordings requires neural probes with reduced footprints to minimize tissue damage. One way achieve this is by implementing dense electrode narrower feedline dimensions, though increases susceptibility capacitive coupling between electrical interconnects. To address this, study explores the resolution limits flexible MEAs optimizing fabrication using optical contact lithography (OCL) and electron beam (EBL). OCL enables metal feedlines widths 520 nm interconnect spaces 280 nm, while EBL allows realization 50 150 on parylene C substrates. Based these techniques, we fabricate a 64‐channel intracortical implant miniaturized cross‐section only × 6 or 70 µm 2 . In vivo validation in awake rats demonstrates that fabricated, implants submicron offer low‐impedance electrodes crosstalk, enabling reliable neuronal recordings. These findings demonstrate feasibility miniaturizing single‐metal layer process, thereby reducing manufacturing complexity thin‐film polymer‐based interfaces.

Language: Английский

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In vivo microelectrode arrays for neuroscience

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: May 8, 2025

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Electrogelation of PEDOT:PSS and its copolymer for bioelectronics

Journal of Materials Chemistry C, Journal Year: 2024, Volume and Issue: 12(37), P. 14944 - 14954

Published: Jan. 1, 2024

This paper explores the combination of screen-printing and electrogelation PEDOT:PSS, including non-solution-processable PEDOT:PSS-copolymers, to fabricate scalable cost-effective coated devices for bioelectronic applications.

Language: Английский

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