Advanced Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 4, 2024
Abstract
A
deeper
understanding
of
disease
heterogeneity
highlights
the
urgent
need
for
precision
medicine.
Microfluidics,
with
its
unique
advantages,
such
as
high
adjustability,
diverse
material
selection,
low
cost,
processing
efficiency,
and
minimal
sample
requirements,
presents
an
ideal
platform
medicine
applications.
As
nanoparticles,
both
biological
origin
therapeutic
purposes,
become
increasingly
important
in
medicine,
microfluidic
nanoparticle
separation
proves
particularly
advantageous
handling
valuable
samples
personalized
This
technology
not
only
enhances
detection,
diagnosis,
monitoring,
treatment
accuracy,
but
also
reduces
invasiveness
medical
procedures.
review
summarizes
fundamentals
techniques
starting
examination
properties
essential
core
principles
that
guide
various
methods.
It
then
explores
passive,
active,
hybrid
techniques,
detailing
their
principles,
structures,
Furthermore,
contributions
to
advancements
liquid
biopsy
nanomedicine.
Finally,
it
addresses
existing
challenges
envisions
future
development
spurred
by
emerging
technologies
advanced
materials
science,
3D
printing,
artificial
intelligence.
These
interdisciplinary
collaborations
are
anticipated
propel
platformization
significantly
expanding
potential
We
report
the
assembly
of
poly(ethylene
glycol)
nanoparticles
(PEG
NPs)
and
optimize
their
surface
chemistry
to
minimize
formation
protein
coronas
immunogenicity
for
improved
biodistribution.
PEG
NPs
cross-linked
with
disulfide
bonds
are
synthesized
utilizing
zeolitic
imidazolate
framework-8
as
templates,
which
subsequently
modified
molecules
different
end
groups
(carboxyl,
methoxy,
or
amino)
vary
chemistry.
Among
modifications,
amino
residual
carboxyl
form
a
pair
zwitterionic
structures
on
NPs,
adsorption
proteins
(e.g.,
immunoglobulin,
complement
proteins)
maximize
blood
circulation
time.
The
influence
preexisting
antibodies
in
mice
pharmacokinetics
is
negligible,
demonstrates
resistance
anti-PEG
inhibition
accelerated
clearance
phenomenon.
This
research
highlights
importance
PEGylated
design
delivery
systems
reveals
translational
potential
cancer
therapy.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(14)
Published: Jan. 28, 2024
Abstract
Intracellular
delivery
of
nano‐drug‐carriers
(NDC)
to
specific
cells,
diseased
regions,
or
solid
tumors
has
entered
the
era
precision
medicine
that
requires
systematic
knowledge
nano‐biological
interactions
from
multidisciplinary
perspectives.
To
this
end,
review
first
provides
an
overview
membrane‐disruption
methods
such
as
electroporation,
sonoporation,
photoporation,
microfluidic
delivery,
and
microinjection
with
merits
high‐throughput
enhanced
efficiency
for
in
vitro
NDC
delivery.
The
impact
characteristics
including
particle
size,
shape,
charge,
hydrophobicity,
elasticity
on
cellular
uptake
are
elaborated
several
types
systems
aiming
hierarchical
targeting
vivo
reviewed.
Emerging
ex
human/animal‐derived
pathophysiological
models
further
explored
highly
recommended
use
studies
since
they
might
mimic
features
fill
translational
gaps
animals
humans.
exploration
modern
microscopy
techniques
precise
nanoparticle
(NP)
tracking
at
cellular,
organ,
organismal
levels
informs
tailored
development
NDCs
application
clinical
translation.
Overall,
integrates
latest
insights
into
smart
nanosystem
engineering,
physiological
models,
imaging‐based
validation
tools,
all
directed
towards
enhancing
efficient
intracellular
NDCs.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(21), P. 27040 - 27054
Published: May 14, 2024
Strong
precorneal
clearance
mechanisms
including
reflex
blink,
constant
tear
drainage,
and
rapid
mucus
turnover
constitute
great
challenges
for
eye
drops
effective
drug
delivery
to
the
ocular
epithelium.
In
this
study,
cyclosporine
A
(CsA)
treatment
of
dry
disease
(DED)
was
selected
as
model
drug.
Two
strategies,
PEGylation
penetration
cationization
potent
cellular
uptake,
were
combined
construct
a
novel
CsA
nanosuspension
(NS@lipid-PEG/CKC)
by
coating
nanoscale
particles
with
mixture
lipids,
DSPE-PEG2000,
cationic
surfactant,
cetalkonium
chloride
(CKC).
NS@lipid-PEG/CKC
mean
size
∼173
nm
positive
zeta
potential
∼+40
mV
showed
promoted
penetration,
good
cytocompatibility,
more
prolonged
retention
without
obvious
irritation.
More
importantly,
recovered
production
goblet
cell
density
efficiently
than
commercial
nanoemulsion
on
rat
model.
All
results
indicated
that
combination
might
provide
promising
strategy
coordinate
uptake
enhanced
epithelium
nanomedicine-based
drops.
Methicillin-resistant
Staphylococcus
aureus
(MRSA)
is
a
refractory
pneumonia-causing
pathogen
due
to
the
antibiotic
resistance
and
characteristics
of
persisting
inside
its
host
cell.
Lysostaphin
typical
bacteriolytic
enzyme
for
degrading
bacterial
cell
walls
via
hydrolysis
pentaglycine
cross-links,
showing
potential
combat
multidrug-resistant
bacteria.
However,
there
are
still
grand
challenges
native
lysostaphin
because
poor
shelf
stability
limited
bioavailability.
To
tackle
these
limitations,
modular
assembly
strategy
proposed
actively
engineer
lysostaphin,
involving
nanoassembly
preparation
fusing
with
lysine-rich
polypeptide.
The
engineered
lysine
component
significantly
improves
membrane-penetration
capability
greatly
increasing
intracellular
antibacterial
activity
by
12-fold
compared
wild-type
lysostaphin.
Notably,
half-life
nanoassembled
approximately
13
times
longer
than
that
counterpart,
outperforming
other
studies.
Most
importantly,
our
improved,
retaining
over
99.9%
after
12
weeks
at
room
temperature.
This
successfully
enhances
overall
performance
offering
great
promise
platform
technique
refine
enzymatic
material
widespread
clinical
demands.
International Journal of Nanomedicine,
Journal Year:
2025,
Volume and Issue:
Volume 20, P. 2813 - 2846
Published: March 1, 2025
Abstract:
Metal-based
nanoparticles
(MNPs)
have
great
potential
for
applications
in
wound
healing
and
tissue
engineering,
due
to
their
unique
structures,
high
bioactivities,
excellent
designability
characteristics,
an
increasing
number
of
studies
been
devoted
modifying
these
species
generate
novel
composites
with
desirable
optical,
electrical,
magnetic
properties.
However,
few
systematic
detailed
reviews
performed
relating
the
modification
approaches
available
MNPs
resulting
composites.
In
this
review,
a
comprehensive
summary
is
regarding
optimized
formulations
application
dressings,
techniques
used
prepare
composite
dressings
are
discussed.
addition,
safety
profiles
nanocomposite
limitations
reported
systems
evaluated.
More
importantly,
solution
strategies
proposed
address
limitations.
Overall,
review
provides
new
ideas
design
facilitate
field
skin
repair,
also
looks
into
future
direction
biomedical
field.
Keywords:
metal-based
nanoparticles,
nanocomposite,
dressing,
multi-functional,
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(7), P. 4329 - 4343
Published: June 4, 2024
The
development
of
nanotherapy
targeting
mitochondria
to
alleviate
oxidative
stress
is
a
critical
therapeutic
strategy
for
vascular
calcification
(VC)
in
diabetes.
In
this
study,
we
engineered
mitochondria-targeted
nanodrugs
(T4O@TPP/PEG–PLGA)
utilizing
terpinen-4-ol
(T4O)
as
natural
antioxidant
and
mitochondrial
protector,
PEG–PLGA
the
nanocarrier,
triphenylphosphine
(TPP)
ligand.
vitro
assessments
demonstrated
enhanced
cellular
uptake
T4O@TPP/PEG–PLGA,
with
effective
targeting.
This
nanodrug
successfully
reduced
induced
by
high
glucose
levels
smooth
muscle
cells.
vivo
studies
showed
prolonged
retention
nanomaterials
thoracic
aorta
up
24
h.
Importantly,
experiments
diabetic
VC
models
underscored
potent
properties
evidenced
its
ability
mitigate
restore
morphology.
These
results
suggest
that
these
could
be
promising
managing
VC.
