Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration DOI Creative Commons
Haozhen Ren, Danqing Huang, Mengdi Qiu

et al.

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(4)

Published: Dec. 1, 2024

Abstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fish extracellular matrix regeneration. By decellularizing combining it with gelatin methacryloyl, scaffold retains essential endogenous growth factors such as collagen glycosaminoglycans. Additionally, microfluidic‐assisted technology enables precise modulation composition architecture to fulfill clinical requirements. Benefiting source materials, exhibit excellent biocompatibility cellular proliferation capacity induced pluripotent stem cell‐derived hepatocytes (iPSC‐heps). Furthermore, macroscopic biomechanical environment foster optimal functional expression iPSC‐heps. Importantly, post‐transplantation, significantly enhance survival rates function mice acute failure, promoting regeneration repair. These findings suggest that printed represent promising candidates transplantation recovery.

Language: Английский

PEDOT‐Integrated Fish Swim Bladders as Conductive Nerve Conduits DOI Creative Commons
Hui Zhang, Dongyu Xu, Bin Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)

Published: June 17, 2024

Abstract Advanced artificial nerve conduits offer a promising alternative for injury repair. Current research focuses on improving the therapeutic effectiveness of by optimizing scaffold materials and functional components. In this study, novel poly(3,4‐ethylenedioxythiophene) (PEDOT)‐integrated fish swim bladder (FSB) is presented as conductive conduit with ordered topology electrical stimulation to promote regeneration. PEDOT nanomaterials adhesive peptides (IKVAV) are successfully incorporated onto decellularized FSB substrate through pre‐coating polydopamine. The obtained PEDOT/IKVAV‐integrated exhibits outstanding mechanical properties, high conductivity, stability, well excellent biocompatibility bioadhesive properties. vitro studies confirm that can effectively facilitate growth directional extension pheochromocytoma 12 cells dorsal root ganglion neurites. addition, in vivo experiments demonstrate proposed accelerate defective repair restoration. findings indicate FSB‐derived multiple regenerative inducing signals integration provide conducive milieu regeneration, exhibiting great potential repairing long‐segment neural defects.

Language: Английский

Citations

7
Acoustic Transmitted Decellularized Fish Bladder For Tympanic Membrane Regeneration DOI Creative Commons
Hong Chen, Hui Zhang, Gang Zhu

et al.

Research, Journal Year: 2025, Volume and Issue: 2025

Published: Jan. 13, 2025

Developing advanced tissue-engineered membranes with biocompatibility, suitable mechanical qualities, and anti-fibrotic anti-inflammatory actions is important for tympanic membrane (TM) repair. Here, we present a novel acoustically transmitted decellularized fish swim bladder (DFB) loaded mesenchymal stem cells (DFB@MSCs) TM perforation (TMP) The DFB scaffolds are obtained by removing the cellular components from original FB, which retains collagen composition that favors cell proliferation. Benefitting their spatially porous structures excellent properties, can provide microenvironment support growth tissue regeneration. In addition, loading on scaffolds, resultant DFB@MSCs system exhibits remarkable effects, together ability to promote migration angiogenesis. vivo experiments confirm prepared not only alleviate inflammatory response caused TMP but also new vessel formation, repair, hearing improvement. These features indicate our proposed stent prospective tool clinical repair of TM.

Language: Английский

Citations

0
Fabrication of Piezoelectric/Conductive Composite Nerve Conduits for Peripheral Nerve Regeneration DOI

Taotao Bian,

Yuhui Jiang, Jie Cao

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 250, P. 114544 - 114544

Published: Feb. 5, 2025

Language: Английский

Citations

0
Bioadhesive drug-loaded microparticles prolong drug retention in the middle ear and ameliorate cisplatin-induced hearing loss DOI Creative Commons
Zhibin Zhou,

Xue Bai,

Zhiyong Zhou

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113728 - 113728

Published: April 1, 2025

Language: Английский

Citations

0
Polymer Scaffolds for peripheral nerve injury repair DOI

Shuhang Yang,

Li Chen, Changning Bai

et al.

Progress in Materials Science, Journal Year: 2025, Volume and Issue: unknown, P. 101497 - 101497

Published: April 1, 2025

Language: Английский

Citations

0
Decellularized Biohybrid Nerve Promotes Motor Axon Projections DOI Creative Commons
Abijeet Singh Mehta, Sophia Zhang,

Xinran Xie

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Abstract Developing nerve grafts with intact mesostructures, superior conductivity, minimal immunogenicity, and improved tissue integration is essential for the treatment restoration of neurological dysfunctions. A key factor promoting directed axon growth into grafts. To achieve this, biohybrid nerves are developed using decellularized rat sciatic modified by in situ polymerization poly(3,4‐ethylenedioxythiophene) (PEDOT). Nine compared varying conditions cycles, selecting best candidate through material characterization. These results show that a 1:1 ratio FeCl 3 oxidant to ethylenedioxythiophene (EDOT) monomer, cycled twice, provides conductivity (>0.2 mS cm −1 ), mechanical alignment, high compatibility cells blood. test nerve's effectiveness motor growth, human Spinal Cord Spheroids (hSCSs) derived from HUES Hb9:GFP used, axons labeled green fluorescent protein (GFP). Seeding hSCS onto one end conduit allows outgrowth nerve. The construct effectively promotes which improves significantly after seeding Schwann cells. This study presents promising approach reconstructing axonal tracts humans.

Language: Английский

Citations

2
Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration DOI Creative Commons
Haozhen Ren, Danqing Huang, Mengdi Qiu

et al.

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(4)

Published: Dec. 1, 2024

Abstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fish extracellular matrix regeneration. By decellularizing combining it with gelatin methacryloyl, scaffold retains essential endogenous growth factors such as collagen glycosaminoglycans. Additionally, microfluidic‐assisted technology enables precise modulation composition architecture to fulfill clinical requirements. Benefiting source materials, exhibit excellent biocompatibility cellular proliferation capacity induced pluripotent stem cell‐derived hepatocytes (iPSC‐heps). Furthermore, macroscopic biomechanical environment foster optimal functional expression iPSC‐heps. Importantly, post‐transplantation, significantly enhance survival rates function mice acute failure, promoting regeneration repair. These findings suggest that printed represent promising candidates transplantation recovery.

Language: Английский

Citations

0