Remodeling the Senescent Microenvironment for Promoting Osteoporotic Tendon-to-Bone Healing via Synergizing Senolytic Quercetin and Aligned Nanowire-Structured Hydrogels DOI
Wei Song, Wencai Liu, Siyi Li

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Osteoporotic tendon-to-bone healing remains a major challenge, as cellular senescence disrupts tissue regeneration and impairs repair outcomes. Although the role of in rotator cuff is increasingly recognized, current strategies often overlook complex pathological context, particularly dual impacts on both bone marrow-derived mesenchymal stem cells (BMSCs) tendon-derived (TDSCs). This gap hampers effective integration, especially under osteoporotic conditions. Herein, composite hydrogel system, quercetin-loaded aligned ultralong hydroxyapatite nanowire/gelatin-hyaluronic acid (Que-AHNW/GH), has been developed to address these challenges. By integrating senolytic quercetin biological cue with highly (HAP) nanowires topographical cue, system remodels senescent microenvironment, alleviating BMSCs TDSCs promoting osteogenesis tenogenesis. Que-AHNW/GH suppresses PI3K/AKT pathway, enhances autophagy, reduces cell types. In vivo, improves tunnel regeneration, tendon repair, integration rats injury. biomechanical strength gait performance demonstrates excellent biosafety. These findings highlight promising potential multifunctional biomaterial for effectively senescence-related healing, offering solution treating injuries.

Language: Английский

A deformable SIS/HA composite hydrogel coaxial scaffold promotes alveolar bone regeneration after tooth extraction DOI Creative Commons
Shiqing Ma, Yumeng Li, Shiyu Yao

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 46, P. 97 - 117

Published: Dec. 10, 2024

Language: Английский

Citations

4
ROS‐Activated Nanohydrogel Scaffolds with Multi‐Factors Controlled Release for Targeted Dual‐Lineage Repair of Osteochondral Defects DOI Creative Commons
Xiuhui Wang,

Shunli Wu,

Ruiyang Li

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

Abstract Achieving self‐healing for osteochondral defects caused by trauma, aging, or disease remains a significant challenge in clinical practice. It is an effective therapeutic strategy to construct gradient‐biomimetic biomaterials that replicate the hierarchical structure and complex microenvironment of tissues dual‐lineage regeneration both cartilage subchondral bone. Herein, ROS‐activated nanohydrogels composite bilayer scaffolds with multi‐factors controlled release are rationally designed using combination 3D printing gelatin placeholder methods. The resulting nanohydrogel exhibit micro‐nano interconnected porous soft‐hard mechanical strength facilitating culture BMSCs vitro. More importantly, multi‐stage continuous responses anti‐inflammation, chondrogenesis osteogenesis, effectively induced via sequential multi‐factors, including diclofenac sodium (DS), kartogenin (KGN) bone morphogenetic protein 2 (BMP‐2), from scaffolds, thereby improved tissue defect model SD rats. These findings suggest such specific delivery functional factors, provides promising defects.

Language: Английский

Citations

0
Enhanced Osteogenic Differentiation of hMSCs Using BMP@ZIF-8-Loaded GelMA Nanocomposite Hydrogels with Controlled BMP-2 Release DOI Creative Commons
Weihao Yuan, Luiza de Almeida Queiroz Ferreira,

Ronit Khade

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Hydrogels are highly versatile materials with immense potential for tissue engineering and regenerative medicine owing to their biocompatibility, tunable mechanical properties, ability mimic the natural extracellular matrix. Their 3D porous structure allows encapsulation delivery of bioactive molecules, making them ideal candidates drug systems. In repair, particularly bone regeneration, hydrogels can serve as carriers that release therapeutic agents in a controlled manner, thus enhancing healing process. Zeolitic Imidazolate Framework-8 (ZIF-8) nanoparticles recombinant human Bone Morphogenetic Protein (rhBMP-2) molecules were incorporated solely (ZIF@GelMA) or association (BMP@ZIF@GelMA) into gelatin modified by methacryloyl hydrogel (GelMA) investigate its physical osteogenic properties. characterized Scanning Electron Microscopy rheological tests. We analyzed degradation BSA profile BMP@ZIF@GelMA samples throughout 0, 1, 3, 7, 14, 28 days. Cell adhesion formation markers hydrogel-encapsulated dental pulp cells using immunocytochemistry molecular analysis. ZIF@GelMA exhibited viscoelastic increased storage modulus when rhBMP2 was present. BSA@ZIF@GelMA showed balanced rate BSA. The upregulated expression cell genes, BMP-2 introduced, levels remarkably elevated. presents several favorable factors promote cellular encouraging further prospects advanced applications repair.

Language: Английский

Citations

0
Remodeling the Senescent Microenvironment for Promoting Osteoporotic Tendon-to-Bone Healing via Synergizing Senolytic Quercetin and Aligned Nanowire-Structured Hydrogels DOI
Wei Song, Wencai Liu, Siyi Li

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Osteoporotic tendon-to-bone healing remains a major challenge, as cellular senescence disrupts tissue regeneration and impairs repair outcomes. Although the role of in rotator cuff is increasingly recognized, current strategies often overlook complex pathological context, particularly dual impacts on both bone marrow-derived mesenchymal stem cells (BMSCs) tendon-derived (TDSCs). This gap hampers effective integration, especially under osteoporotic conditions. Herein, composite hydrogel system, quercetin-loaded aligned ultralong hydroxyapatite nanowire/gelatin-hyaluronic acid (Que-AHNW/GH), has been developed to address these challenges. By integrating senolytic quercetin biological cue with highly (HAP) nanowires topographical cue, system remodels senescent microenvironment, alleviating BMSCs TDSCs promoting osteogenesis tenogenesis. Que-AHNW/GH suppresses PI3K/AKT pathway, enhances autophagy, reduces cell types. In vivo, improves tunnel regeneration, tendon repair, integration rats injury. biomechanical strength gait performance demonstrates excellent biosafety. These findings highlight promising potential multifunctional biomaterial for effectively senescence-related healing, offering solution treating injuries.

Language: Английский

Citations

0