Co‐aggregation as A Simple Strategy for Preparing Fluorogenic Tetrazine Probes with On‐Demand Fluorogen Selection** DOI
S. Segawa, Jiajie Wu, Ryan T. K. Kwok

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 63(11)

Published: Dec. 7, 2023

Life science has progressed with applications of fluorescent probes-fluorophores linked to functional units responding biological events. To meet the varied demands across experiments, simple organic reactions connect fluorophores and have been developed, enabling on-demand selection fluorophore-functional unit combinations. However, synthesis requires professional equipment skills, standing as a daunting task for life scientists. In this study, we present simple, fast, convenient strategy probe preparation: co-aggregation hydrophobic molecules. We focused on tetrazine-a difficult-to-prepare yet useful that provides effective bioorthogonal reactivity strong fluorogenicity. Simply mixing tetrazine molecules aggregation-induced emission (AIE) luminogens in water, is induced, AIE quenched. Subsequent click reaction bioorthogonally turns emission, identifying these coaggregates fluorogenic probes. Thanks fluorogenicity, established new time-gated fluorescence bioimaging technique distinguish overlapping signals, multi-organelle imaging two same-color fluorophores. Our study showcases potential method preparation probes well protocols molecular design principles approach, offering an solution evolving needs research.

Language: Английский

Bioorthogonal Guided Activation of cGAS‐STING by AIE Photosensitizer Nanoparticles for Targeted Tumor Therapy and Imaging DOI

Minhui Cui,

Dongsheng Tang, Bin Wang

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(52)

Published: Sept. 5, 2023

Abstract Photodynamic therapy (PDT) and photothermal (PTT) leverage reactive oxygen species (ROS) control local hyperthermia by photosensitizer to perturb intracellular redox equilibrium, inducing DNA damage in both mitochondria nucleus, activating the cGAS‐STING pathway, ultimately eliciting antitumor immune responses. However, current photosensitizers are encumbered limitations such as suboptimal tumor targeting, aggregation‐caused quenching (ACQ), restricted excitation emission wavelengths. Here, this work designs novel nanoparticles based on aggregation‐induced (AIE) (BODTPE) for targeted near‐infrared II fluorescence imaging (NIR‐II FLI) with enhanced PDT/PTT effects. BODTPE is employed a monomer, dibenzocyclooctyne (DBCO)‐PEG 2k ‐amine serving an end‐capping polymer, synthesize BODTPE‐containing polymer (DBD). Further, through self‐assembly, DBD mPEG‐DSPE combined form (NP‐DBD). Notably, DBCO surface of NP‐DBD can react azide groups cancer cells pretreated Ac 4 ManNAz copper‐free click reaction. This innovative formulation led accumulation within sites, phenomenon convincingly demonstrated murine models subjected N ‐azidoacetylmannosamine‐tetraacylated (Ac ManNAz) pretreatment. Significantly, exhibits multifaceted effect encompassing PDT/PTT/NIR‐II FLI upon 808 nm laser irradiation, thereby better culminating compelling inhibition augmented robust modulation.

Language: Английский

Citations

67
Recent Advances in Fluorescent Probes for Cancer Biomarker Detection DOI Creative Commons

Mingce Tian,

Riliga Wu,

Caihong Xiang

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(5), P. 1168 - 1168

Published: March 6, 2024

Many important biological species have been identified as cancer biomarkers and are gradually becoming reliable targets for early diagnosis late therapeutic evaluation of cancer. However, accurate quantitative detection remains challenging due to the complexity systems diversity development. Fluorescent probes extensively utilized identifying substances their notable benefits being non-invasive, quickly responsive, highly sensitive selective, allowing real-time visualization, easily modifiable. This review critiques fluorescent used detecting imaging over last five years. Focuses made on design strategies small-molecule nano-sized probes, construction methods fluorescence sensing platforms, further applications in multiple biomarkers, including enzymes, reactive oxygen species, sulfur microenvironments. aims guide development excellent diagnostic promote broad application analysis diagnosis.

Language: Английский

Citations

19
Fungal detection and elimination based on AIEgens DOI
Pu Chen, Jiawen Zhao, Dong‐Mi Li

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 533, P. 216548 - 216548

Published: Feb. 26, 2025

Language: Английский

Citations

2
Recent advances in aggregation-induced emission (AIE)-based chemosensors for the detection of organic small molecules DOI
Ming Hui Chua, Bryan Yat Kit Hui, Kang Le Osmund Chin

et al.

Materials Chemistry Frontiers, Journal Year: 2023, Volume and Issue: 7(22), P. 5561 - 5660

Published: Jan. 1, 2023

This review provides an overview of the progress in development AIE-based organic small molecule probes for detecting biologically important molecules, drug volatile compounds, and explosives over past five years.

Language: Английский

Citations

39
The Role of Structural Hydrophobicity on Cationic Amphiphilic Aggregation-Induced Emission Photosensitizer-Bacterial Interaction and Photodynamic Efficiency DOI
Michelle M. S. Lee, Eric Y. Yu, Dingyuan Yan

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(17), P. 17004 - 17020

Published: Aug. 18, 2023

The aggregation-induced emission photosensitizer (AIE PS) has stood out as an alternative and competent candidate in bacterial theranostics, particularly with the use of cationic AIE PS discrimination elimination. Most reported work emphasizes role electrostatic interaction between negatively charged surfaces, enabling broad applications from to killing. However, underlying targeting mechanism design rationale for effective labeling remain poorly investigated. In this Article, we designed synthesized a series amphiphilic PSs different calculated log P values. Then, systemically studied relationship hydrophobicity variation outcomes, dose needed label various species bacteria, their photodynamic antibacterial efficiency. findings provide better understanding unclear PS-bacterial some insights into structural strategies development theranostics.

Language: Английский

Citations

38
Matthew effect: General design strategy of ultra‐fluorogenic nanoprobes with amplified dark–bright states in aggregates DOI Creative Commons
S. Segawa, Xinwen Ou, Tianruo Shen

et al.

Aggregate, Journal Year: 2024, Volume and Issue: 5(2)

Published: Jan. 4, 2024

Abstract Fluorescence imaging, a key technique in biological research, frequently utilizes fluorogenic probes for precise imaging living systems. Tetrazine is an effective emission quencher probe designs, which can be selectively damaged upon bioorthogonal click reactions, leading to considerable enhancement. Despite significant efforts increase the enhancement ratio ( I AC / BC ) of tetrazine‐functionalized probes, influence molecular aggregation on properties has been largely overlooked these designs. In this study, we reveal that ultrahigh realized aggregate system when tetrazine paired with aggregation‐induced (AIE) luminogens. amplifies its quenching efficiency and drastically reduce background emissions. Subsequent reactions damage trigger AIE, considerably enhanced . We further showcase capability ultra‐fluorogenic systems selective multiple organelles cells. term unique fluorogenicity AIE luminogen‐quencher complexes amplified dark‐bright states as “Matthew effect” emission, potentially providing universal approach attain diverse

Language: Английский

Citations

10
Crescent-shaped Janus nanoassemblies of gold nanoparticles and AIEgens: Enhancing plasmonic and fluorescent activities for colorimetric and ratiometric fluorescence lateral flow immunoassay DOI
Xirui Chen,

Wenchao Zhan,

Hong Duan

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 58, P. 102452 - 102452

Published: Aug. 16, 2024

Language: Английский

Citations

8
Bioorthogonally activated probes for precise fluorescence imaging DOI Creative Commons
Youxin Fu, Xing Zhang, Luling Wu

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

This article provides a comprehensive overview of recent advances in the development bioorthogonally activated probes for labelling and precise situ fluorescence imaging both vitro vivo .

Language: Английский

Citations

5
Simultaneous Detection of Micro-RNAs by a Disposable Biosensor via the Click Chemistry Connection Strategy DOI
Xiaofang Liu, Qun Wang, Jiawei Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10577 - 10585

Published: June 18, 2024

Simultaneous detection of multiple breast cancer-associated miRNAs significantly raises the accuracy and reliability early diagnosis. In this work, disposable carbon fiber paper serves as biosensing interface, linking DNA probes via click chemistry to efficiently capture targets signals efficiently. have recognition domains that trigger a cascade reaction through helper targets, resulting in two output. The are centrally encapsulated pore MIL-88(Fe)-NH2. signal carriers directed by correspond probes. biosensor is selective stable, it can quantify miRNA-21 miRNA-155 simultaneously with limits 0.64 0.54 fmol/L, respectively. Furthermore, demonstrates satisfactory performance tests conducted normal human serum cell lysate. Overall, method makes exploration realize an inexpensive sensitive for biomarkers.

Language: Английский

Citations

4
Recent advances of aggregation-induced emission luminogens for point-of-care biosensing systems DOI

Xiaoxia Jian,

Guoyu Jiang, Jianguo Wang

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(65), P. 8484 - 8496

Published: Jan. 1, 2024

Aggregation-induced emission (AIE)-based POC detection is important for diagnosis of diseases and assessment the effectiveness treatment programs. This review summarized advancement design application AIE-based biosensors.

Language: Английский

Citations

4