Ultrabright NIR AIEgen nanoparticles‐enhanced lateral flow immunoassay platform for accurate diagnostics of complex samples

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: April 10, 2024

Abstract Accurate and sensitive near‐infrared (NIR) luminescent lateral flow immunoassay (LFIA) has attracted considerable attention in the field of point‐of‐care testing (POCT). However, detection accuracy sensitivity are often compromised by low fluorescence quantum efficiency (<10%) NIR fluorescent probe. Herein, ultrabright AIEgen nanoparticles (PS@AIE 830 NPs) composed polystyrene (PS) aggregation‐induced emission luminogen (AIEgen) with maximum at nm (AIE ) is reported, its potential to promote an accurate complex samples LFIA described. The relative yield (QY) PS@AIE NPs was 14.76%, which superior that polymer embedding method indocyanine green (ICG)‐based nanoparticles. immunolabeled‐LFIA combined laboratory‐built NIR‐LFIA portable quantitative instruments (detected light range > 800 nm) completely eliminated background interference allowed highly without any pre‐treatment steps. limits (LODs) for aflatoxin B 1 (AFB soy sauce, alpha hemolysin (Hla) Staphylococcus aureus biomarker joint fluid, C‐reactive protein (CRP) human haemolysed were 0.01 ng mL −1 , 0.02 µg 0.156 mg L respectively, commensurating those corresponding gold standard assays covering interests. It anticipated will serve as a universally applicable signal probe diagnostics, promising expand applications samples.

Language: Английский

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A Rapidly Synthesized, Ultrasmall Silver Nanocluster for Near-Infrared-II Imaging and Metabolic Studies

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Near-infrared-II (NIR-II) imaging has emerged as a powerful technique for high-resolution visualization of deep anatomical features, benefiting from minimized autofluorescence, diminished optical scattering, and absorption tissue. However, the current synthesis NIR-II nanoprobes is time-consuming, labor-intensive process with low yields, highlighting need an efficient rapid approach instead. Herein, we report DNA-templated silver nanoclusters (Ag NCs) emission that can be rapidly synthesized via simple one-spot within 2 min. The Ag NCs are about 1.6 nm in size, making it easy them to enter into capillaries muscle In vivo results indicate designed promising probes studying metabolic pathways after intramuscular injection. Therefore, expected ultrafast room temperature synthesis, excellent emission, ultrasmall size will ideal biological applications.

Language: Английский

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Gold Nanoclusters as High Resolution NIR-II Theranostic Agents

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(7), P. 462 - 480

Published: June 18, 2024

In the realm of nanomaterials, atomically precise quasi-molecular gold nanoclusters (AuNCs) play a prime role due to their unique, stable, and highly tunable optical properties. They are extensively structure-engineered for modulation surface electronic states toward long wavelength photoluminescence, particularly in NIR-II (1000 1700 nm) window. Contrast agents with emission can potentially transform imaging terms higher spatial resolution, deeper tissue penetration, reduced autofluorescence. These advantages allow real-time living organisms observing disease progression treatment response. this short review, we discuss origin rationally designed AuNCs application high resolution vasculatures hard soft structures identification pathological conditions such as stroke injury. Further, recent employment these rapidly growing field tumor theranostics is also summarized. Final remarks provided on scope improvement properties persisting challenges clinical translation.

Language: Английский

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Molecular Etching‐Derived High‐Brightness NIR‐II Gold Nanoclusters for High‐Resolution Bioimaging and Photothermal Therapy

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Glutathione‐capped gold nanoclusters (GSH‐Au NCs) with second near‐infrared (NIR‐II, 1000–1700 nm) fluorescence have received increasing interest in biomedical applications. However, conventional aqueous synthesis of GSH‐Au NCs results low NIR‐II absolute quantum yield (≈0.27%) and limited renal clearance efficiency (≈70%), which restricts its clinical This study reports a GSH‐etching post‐synthesis method to selectively convert nonfluorescent n ( > 25) into fluorescent 25 (eGSH‐Au NCs). molecular etching process decreases the size from 3.3 2.0 nm afforded 37% increase 11.9% enhancement efficiency. The optimized eGSH‐Au facilitates whole cerebrovascular imaging through scalp skull, tumor vasculature resolution 40.8 µm, dynamic perfusion signal‐to‐background ratio 10.5, enhanced lymphatic vessel lymph node imaging. Additionally, exhibit superior photothermal properties, achieving almost 100% growth inhibition Hepa1‐6 tumor‐bearing mice. presents novel strategy improve bioimaging therapeutic performance Au NCs, thus advancing their translation.

Language: Английский

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Engineering Cu nanoclusters with aggregation-induced emission for photodynamic healing of wound with drug-resistant bacteria-infection

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 492, P. 152216 - 152216

Published: May 13, 2024

Language: Английский

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Multiscale Bioresponses of Metal Nanoclusters

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(13)

Published: Dec. 25, 2023

Abstract Metal nanoclusters (NCs) are well‐recognized novel nano‐agents that hold great promise for applications in nanomedicine because of their ultrafine size, low toxicity, and high renal clearance. As foreign substances, however, an in‐depth understanding the bioresponses to metal NCs is necessary but still far from being realized. Herein, this review deployed summarize biofates at various biological levels, emphasizing multiscale molecular, cellular, organismal levels. In parts‐to‐whole schema, interactions between biomolecules discussed, presenting typical protein‐dictated nano–bio interfaces, hierarchical structures, vivo trajectories. Then, accumulation, internalization, metabolic evolution cellular environment as‐imparted theranostic functionalization demonstrated. The metabolism transportation processes subsequently distilled. Finally, ends with conclusions perspectives on outstanding issues NC‐mediated near future. This expected provide inspiration tailoring customization NC‐based meet practical requirements different sectors nanomedicine.

Language: Английский

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Spacer Twisting Strategy to Realize Ultrabright Near-Infrared II Polymer Nanoparticles for Fluorescence Imaging-Guided Tumor Phototheranostics

ACS Nano, Journal Year: 2024, Volume and Issue: 18(41), P. 28178 - 28188

Published: Oct. 3, 2024

Conjugated polymers are becoming popular near-infrared II (NIR-II) phototheranostic agents (PTAs) due to their numerous advantages, such as high photostability, large molar extinction coefficients, and excellent photothermal properties. However, the strong π-π interactions between chains of conjugated resulted in generally low NIR-II emission quantum yields (QY). Therefore, synthesis with QY is an interesting but challenging task. Herein, we proposed a spacer twisting strategy realize ultrabright polymer nanoparticles for fluorescence imaging-guided tumor phototheranostics. Theoretical calculations indicated that PY-IT has largest dihedral angle largely π-conjugated skeleton spacer, which can effectively inhibit intermolecular stacking, resulting improved 16.5% nanoparticles. In addition, NPs perform imaging treatment tumors. The work presents some valuable guides achieving polymeric PTAs QY.

Language: Английский

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Fabrication of conjugated polymers with aggregation-induced near-infrared-II emission for efficient phototheranostics

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(34), P. 8383 - 8388

Published: Jan. 1, 2024

Conjugated polymers (CPs), which emit in the second near-infrared window (NIR-II, 1000-1700 nm), are used as biomaterials for NIR-II fluorescence imaging because of their adjustable photophysical properties and high optical stability. However, signal conventional CPs is quenched an aggregated state due to strong π-π stacking, results closure radiation attenuation pathway. To solve this problem, aggregation-induced emission effect considered a reasonable strategy enhancing aggregative IR-II emitters. We herein report conjugated with typical AIE characteristics (αAIE > 3) by changing side chain structure receptor units conjugation degree donors. polymer nanoparticles (PoBVT NPs) exhibit outstanding performance (QY = 1.94%) highly effective photothermal therapy (η 45%). In vivo studies have shown that location tumors can be accurately obtained FL/NIR-II PA imaging, there significant anti-tumor after laser irradiation. This work offers prospects design multifunctional FL/PA guide PTT applications.

Language: Английский

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Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Fluorescence signal “turn‐on” lateral flow immunoassay (FONLFA) through nanomaterial labeled quenching fluorescent has shown significant potential for the detection of small molecules. However, immobilization on nitrocellulose (NC) membrane commonly requires tedious chemical modification and only a few combinations fluorescence donor quencher have been applied in FONLFA. In this work, bright metal nanoclusters (Prot‐AuNCs) were prepared self‐assembled into Prot‐AuNCs/antigen aggregates with three typical molecule antigens, respectively. The can be readily immobilized surface NC membrane, indicating that strip fabrication strategy good versatility. Moreover, we evaluated performances FONLFA platform by using carbendazim as model target investigated four nanomaterials colorimetric nanoprobes quenchers. We found all demonstrated significantly improved naked eye sensitivity (vLOD) limits (LODs) quantitative analysis. Among them, combing Fe‐polydopamine nanoparticles above aggregates, mode achieved 200‐fold vLOD (0.05 ng mL −1 ) compared conventional AuNPs‐based (AuNPs‐LFA) (10 ). addition, LOD analysis also was 22‐fold whole test process completed within 10 min. With advantages efficient fabrication, extraordinary sensitization, biocompatibility, our is expected to great rapid various

Language: Английский

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Modulating the photodynamic modality of Au₂₂ nanoclusters through surface conjugation of arginine for promoted healing of bacteria-infected wounds

Nanoscale, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

An Au nanocluster (NC)-based photodynamic antibacterial is designed by conjugating arginine on the surface of 22 NCs, achieving healing bacteria-infected wounds via producing reactive oxygen species (ROS) and nitrogen (RNS).

Language: Английский

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