A mitochondrial targeted fluorescent probe for imaging nitroreductase activity and photodynamic therapy in tumor cells DOI Creative Commons

Ya-Ting Bao,

Haibo Mao,

Kui Lei

et al.

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127392 - 127392

Published: Dec. 18, 2024

The hypoxic environment in tumors is closely linked to tumor structure, function, dissemination, invasion, metastasis, and drug resistance. Nitroreductase (NTR) often recognized as a biomarker evaluate the hypoxia degree for cells. Traditional detection methods such PET, MRI multispectral photoacoustic tomography have limitations. Fluorescent probes garnered attention due their high sensitivity, rapid response, specificity, non-invasive nature. In this study, we introduced novel small molecule fluorescent probe, T-TPE-NO

Language: Английский

Fluorescence-guided Surgery for Hepatocellular Carcinoma: From Clinical Practice to Laboratories DOI Open Access

Tian Xiao,

Didi Chen, Peng Li

et al.

Journal of Clinical and Translational Hepatology, Journal Year: 2025, Volume and Issue: 000(000), P. 000 - 000

Published: Jan. 2, 2025

Fluorescence navigation is a novel technique for accurately identifying hepatocellular carcinoma (HCC) lesions during hepatectomy, enabling real-time visualization. Indocyanine green-based fluorescence guidance has been commonly used to demarcate HCC lesion boundaries, but it cannot distinguish between benign and malignant liver tumors. This review focused on the clinical applications limitations of indocyanine green, as well recent advances in fluorescent probes fluorescence-guided surgery HCC. It covers traditional imaging such enzymes, reactive oxygen species, sulfur pH-sensitive probes, followed by an introduction aggregation-induced emission probes. Aggregation-induced exhibit strong fluorescence, low background signals, excellent biocompatibility, high photostability aggregate state, show no dilute solutions. Design strategies these may offer insights developing identification surgery.

Language: Английский

Citations

0
Aggregation Induced Emissive Liquid Crystals‐Polymer Composite Membrane for Dual‐Channel Analysis of Parathyroid Hormone DOI Creative Commons

Yingping Tong,

Birong Wang,

Dongyu Zhao

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

ABSTRACT Parathyroid hormone (PTH) is an important factor in maintaining blood calcium levels the human body. Therefore, monitoring PTH essential for assessing various diseases progression and managing overall health. In this study, a fluorescence optical sensor based on aggregation‐induced emissive liquid crystal photopolymer (AIE‐LC‐Poly) film was established qualitative quantitative detection of PTH. The specific interaction between anti‐PTH substrate surface utilized, variations orientation aggregation state fluorescent LCs were evaluated by both optic means. limit using image above 10 µg/mL, while achieved much lower 1 ng/mL. Additionally, further amplified signals strengthening AIE effect initiate state, enhancing disturbance ordered upon addition. Ultimately, limits reduced to 0.01 µg/mL 50 pg/mL detection. sensitive AIE‐LC‐Poly biosensing technology presented here sets stage develop LC‐based biomedical applications without labeling.

Language: Английский

Citations

0
Harnessing Aggregation‐Induced Emission‐Based Detection Toolbox for Diagnostics of Urogenital Tumors DOI Creative Commons
Wenzhe Chen,

Hantian Guan,

Yongfeng Lu

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

ABSTRACT Urogenital system tumors include prostate cancer, bladder ovarian and other very common solid tumor diseases with high morbidity mortality. The unique physiological anatomical features of the urogenital render it particularly amenable to application tissue imaging techniques for diagnostic purposes. advancement aggregation‐induced emission (AIE) materials has addressed limitations associated conventional fluorescent that are prone aggregation‐caused quenching. This facilitated development innovative AIE characterized by enhanced photostability, an increased signal‐to‐noise ratio, improved quality. article reviews research progress biosensors in diagnosis tumors. It mainly involves biomarker vitro fluorescence such as uterine which based on biosensors. In addition, a comprehensive description biosensors’ synthesis strategies is provided. includes detailed elucidation platforms intracellular mechanisms basic principles AIE, accompanied presentation quantitative analysis cell results. limitations, challenges suggestions field summarized, prospect prospected. tumors, also provides catalyst exploring characteristics its wide disease diagnosis.

Language: Английский

Citations

0
Wide‐Spectrum Nano‐Antibiotics Based on TPA‐Py@AuNCs⊂BSA for Multimodal Synergistic Therapy of Drug‐Resistant Bacteria and Wound Infections DOI Creative Commons

Fengrui Xu,

Ge Zhang, Kai Zhang

et al.

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

ABSTRACT To meet the high requirements of biomedical applications in antimicrobial agents, it is crucial to explore efficient nano‐antimicrobial agents with no resistance and good biocompatibility for treating infected wounds. In this study, composite nano‐antibiotic TPA‐Py@AuNCs⊂BSA nanoparticles (TAB NPs) are prepared using hollow mesoporous Au nanocages (AuNCs) loaded a photosensitizer (namely TPA‐Py) D‐π‐A structure showing aggregation‐induced emission properties. When TPA‐Py encapsulated cavity AuNCs, its fluorescence suppressed. presence photothermal induction, can be released from allowing restoration illumination specific imaging Gram‐positive bacteria. TAB NPs demonstrate outstanding activity against variety bacteria, multimodal property does not lead development bacterial resistance. vitro experiments show that could eliminate bacteria ablate biofilm. vivo synergistic effect has significant positive impact on treatment wounds, including rapid antibacterial action, promotion M2 macrophage polarization, enhancement chronic wound healing. This study provides an effective strategy developing wide‐spectrum nano‐antibiotics ablation biofilms

Language: Английский

Citations

2
Split G-Quadruplex Programmed Recyclable AIE-Biosensor for Label-Free Detection of miRNA in Acute Kidney Injury DOI
Wenjie Ma, Tingfei Xie, Jinyan Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(44), P. 17814 - 17823

Published: Oct. 25, 2024

We herein rationally designed a target-recyclable AIE-biosensor based on split G-quadruplex for label-free detection of miRNA in acute kidney injury. Initially, the PG was an "OFF" state, and two segments (G4-a G4-b) G4 were tethered at terminals P1 far away from each other due to rigid duplex structure formed by partially complementary intermediate sequences P2 P1, bringing MG with quenched fluorescence. In presence target, 5'-PO

Language: Английский

Citations

2
A mitochondrial targeted fluorescent probe for imaging nitroreductase activity and photodynamic therapy in tumor cells DOI Creative Commons

Ya-Ting Bao,

Haibo Mao,

Kui Lei

et al.

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127392 - 127392

Published: Dec. 18, 2024

The hypoxic environment in tumors is closely linked to tumor structure, function, dissemination, invasion, metastasis, and drug resistance. Nitroreductase (NTR) often recognized as a biomarker evaluate the hypoxia degree for cells. Traditional detection methods such PET, MRI multispectral photoacoustic tomography have limitations. Fluorescent probes garnered attention due their high sensitivity, rapid response, specificity, non-invasive nature. In this study, we introduced novel small molecule fluorescent probe, T-TPE-NO

Language: Английский

Citations

0