Abstract
Sickle
cell
anaemia
(SCD)
is
a
life-threatening
haematological
disorder
which
predominant
in
sub-Saharan
Africa
and
triggered
by
genetic
mutation
of
the
β-chain
haemoglobin
gene
resulting
substitution
glutamic
acid
with
valine.
This
leads
to
production
an
abnormal
molecule
called
S
(HbS).
When
deoxygenated,
(HbS)
polymerizes
results
sickle-shaped
red
blood
rigid
has
significantly
shortened
life
span.
Various
reports
have
shown
strong
link
between
oxidative
stress,
inflammation,
immune
response,
pathogenesis
sickle
disease.
The
consequence
these
processes
lead
development
vasculopathy
(disease
vessels)
several
other
complications.
role
system,
particularly
innate
SCD
become
increasingly
clear
recent
years
research,
However,
little
known
about
roles
adaptive
system
this
review
examines
interaction
transfusion,
their
effects
on
Lab on a Chip,
Journal Year:
2024,
Volume and Issue:
24(4), P. 832 - 842
Published: Jan. 1, 2024
A
high-performance
pure-nickel
magnetically-actuated
microrobot
(Ni-MAR)
is
printed
by
a
‘femtosecond
laser
polymerization
+
sintering’
method
with
great
potential
applications
in
controlled
locomotion
fast-flowing
blood
and
massive
drugs
delivery.
Membranes,
Journal Year:
2022,
Volume and Issue:
12(2), P. 217 - 217
Published: Feb. 13, 2022
In
this
article,
we
describe
the
general
features
of
red
blood
cell
membranes
and
their
effect
on
flow
rheology.
We
first
present
a
basic
description
move
forward
to
membranes’
characteristics
modeling.
later
review
specific
properties
cells,
presenting
recent
numerical
experimental
microfluidics
studies
that
elucidate
elastic
membrane
hemorheology.
Finally,
hemorheological
pathologies
directly
related
mechanical
cells
microcirculation,
reviewing
microfluidic
applications
for
diagnosis
treatment
these
diseases.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(1), P. 117 - 117
Published: Jan. 9, 2023
The
utilizations
of
microfluidic
chips
for
single
RBC
(red
blood
cell)
studies
have
attracted
great
interests
in
recent
years
to
filter,
trap,
analyze,
and
release
erythrocytes
various
applications.
Researchers
this
field
highlighted
the
vast
potential
developing
micro
devices
industrial
academia
usages,
including
lab-on-a-chip
organ-on-a-chip
systems.
This
article
critically
reviews
current
state-of-the-art
advances
microfluidics
analyses,
integrated
sensors
platforms
microscopic/tomographic/spectroscopic
trapping
arrays
(including
bifurcating
channels),
dielectrophoretic
agglutination/aggregation
studies,
as
well
clinical
implications
covering
cancer,
sepsis,
prenatal,
Sickle
Cell
diseases.
Microfluidics
based
microarrays,
sorting/counting
techniques
acoustic,
dielectrophoretic,
hydrodynamic,
magnetic,
optical
techniques)
are
also
reviewed.
Lastly,
organs
on
chips,
multi-organ
drug
discovery
involving
described.
limitations
drawbacks
each
technology
addressed
future
prospects
discussed.
Lab on a Chip,
Journal Year:
2021,
Volume and Issue:
21(6), P. 1036 - 1048
Published: Jan. 1, 2021
Microfluidic
Impedance
Red
Cell
Assay
(MIRCA)
is
integrated
with
capillary
network-inspired
micropillar
arrays
and
electrical
impedance
sensing
electrodes
to
enable
standardized
assessment
of
red
blood
cell-mediated
microvascular
occlusion.
Frontiers in Medicine,
Journal Year:
2022,
Volume and Issue:
9
Published: June 17, 2022
Coronavirus
disease
2019
(COVID-19)
is
caused
by
a
novel
virus
known
as
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
SARS-CoV-2-induced
hyperinflammation
together
with
alteration
of
plasma
proteins,
erythrocyte
deformability,
and
platelet
activation,
may
affect
blood
viscosity.
Thus,
this
review
aimed
to
study
the
link
between
SARS-CoV-2
infection
viscosity
in
COVID-19
patients.
In
order
findings
related
hyperviscosity
COVID-19,
we
suggested
protocol
for
narrative
published
articles.
Hyperviscosity
developed
different
hematological
disorders
including
multiple
myeloma,
sickle
cell
anemia,
Waldenstorm
macroglobulinemia,
polycythemia,
leukemia.
morphology
via
binding
membrane
cluster
differentiation
147
(CD147)
receptors,
B
3
proteins
on
membrane.
Variations
fragility
deformability
endothelial
dysfunction
oxidative
stress
cause
COVID-19.
Of
interest,
poor
tissue
perfusion,
peripheral
vascular
resistance,
thrombosis.
Most
patients
more
than
3.5
cp
develop
coagulation
disorders.
commonly
vaccine
recipients
who
had
formerly
received
due
higher
underlying
immunoglobulin
concentrations,
only
infrequently
those
have
not
vaccine.
Taken
together,
these
observations
are
untimely
too
early
give
final
connotation
vaccination
risk
development
syndrome,
consequently
prospective
retrospective
studies
necessary
regard.
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: Jan. 13, 2023
The
fraction
of
red
blood
cells
adopting
a
specific
motion
under
low
shear
flow
is
promising
inexpensive
marker
for
monitoring
the
clinical
status
patients
with
sickle
cell
disease.
Its
high-throughput
measurement
relies
on
video
analysis
thousands
motions
each
sample
to
eliminate
large
majority
unreliable
samples
(out
focus
or
overlapping
cells)
and
discriminate
between
tank-treading
flipping
motion,
characterizing
highly
poorly
deformable
respectively.
Moreover,
these
videos
are
different
durations
(from
6
more
than
100
frames).
We
present
two-stage
end-to-end
machine
learning
pipeline
able
automatically
classify
in
high
class
imbalance.
By
extending,
comparing,
combining
two
state-of-the-art
methods,
convolutional
neural
network
(CNN)
model
recurrent
CNN,
we
discard
97%
sequences
(first
stage)
accuracy
an
F1-score
0.94
(second
stage).
Dataset
codes
publicly
released
community.
Journal of Molecular Recognition,
Journal Year:
2023,
Volume and Issue:
36(8)
Published: April 24, 2023
Abstract
Mechanical
biomarkers
distinguish
health
conditions
through
quantitative
mechanical
measurements.
The
emergence
and
establishment
of
nanotechnology
in
the
last
decades
have
provided
new
tools
to
obtain
at
nanoscale.
measurements
are
reproducible,
label‐free,
start
be
applied
vivo
can
high
throughput,
require
small
samples.
protocols
clinical
practice
macro
scale
like
palpation
or
blood
pressure
measurement
routinely
used
by
medical
doctors.
Nanotechnology
brought
sensing
next
scale,
where
cells,
tissues,
proteins
probed
linked
conditions.
changes
cells
tissues
may
detected
before
other
markers,
such
as
protein
expression,
providing
an
important
advantage
biomarkers.
In
present
review,
we
explore
biomarker's
historical
evolution,
describe
on
various
diseases
novel
discoveries
nanomechanical
field
for
their
characterization.
We
conclude
that
establishing
hallmarks
diseases,
several
cases
early
diagnostics
discovery
drug
targets
involved
changes,
while
advances
instrumentation
bringing
commercial
products
into
practice.
along
with
testing
niche
market,
whose
demand
is
increasing
due
expansion
personalized
medicine
unmet
needs
clinics.
Biomedicines,
Journal Year:
2023,
Volume and Issue:
11(9), P. 2413 - 2413
Published: Aug. 29, 2023
Sickle
cell
anaemia
(SCD)
is
a
life-threatening
haematological
disorder
which
predominant
in
sub-Saharan
Africa
and
triggered
by
genetic
mutation
of
the
β-chain
haemoglobin
gene
resulting
substitution
glutamic
acid
with
valine.
This
leads
to
production
an
abnormal
molecule
called
S
(HbS).
When
deoxygenated,
(HbS)
polymerises
results
sickle-shaped
red
blood
rigid
has
significantly
shortened
life
span.
Various
reports
have
shown
strong
link
between
oxidative
stress,
inflammation,
immune
response,
pathogenesis
sickle
disease.
The
consequence
these
processes
development
vasculopathy
(disease
vessels)
several
other
complications.
role
system,
particularly
innate
SCD
become
increasingly
clear
recent
years
research;
however,
little
known
about
roles
adaptive
system
this
review
examines
interaction
transfusion,
their
effects
on
anaemia.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: April 17, 2024
Abstract
Pathological
conditions
linked
to
shear
stress
have
been
identified
in
hematological
diseases,
cardiovascular
and
cancer.
These
often
exhibit
significantly
elevated
levels,
surpassing
1000
dyn/cm
2
severely
stenotic
arteries.
Heightened
can
induce
mechanical
harm
endothelial
cells,
potentially
leading
bleeding
fatal
consequences.
However,
current
technology
still
grapples
with
limitations,
including
inadequate
flexibility
simulating
bodily
environments,
limited
range
of
generation,
spatial
temporal
adaptability.
Consequently,
a
comprehensive
understanding
the
mechanisms
underlying
impact
on
physiological
pathological
conditions,
like
thrombosis,
remains
inadequate.
To
address
these
this
study
presents
microfluidic-based
generation
chip
as
proposed
solution.
The
achieves
substantial
929-fold
variation
solely
by
adjusting
degree
constriction
branch
channels
after
PDMS
fabrication.
Experiments
demonstrated
that
rapid
increase
up
detached
88.2%
cells
from
substrate.
Long-term
exposure
(24
h)
levels
below
8.3
did
not
cell
growth.
Furthermore,
exposed
equal
or
greater
than
exhibited
significant
alterations
aspect
ratio
orientation,
following
normal
distribution.
This
microfluidic
provides
reliable
tool
for
investigating
cellular
responses
wide-ranging
existing
both
flow
conditions.
Graphical
Blood Advances,
Journal Year:
2020,
Volume and Issue:
4(15), P. 3688 - 3698
Published: Aug. 10, 2020
Abstract
Sickle
cell
disease
(SCD),
which
afflicts
100
000
Americans,
as
well
millions
worldwide,
is
associated
with
anemia,
lifelong
morbidity,
and
early
mortality.
Abnormal
adhesion
of
sickle
red
blood
cells
(RBCs)
to
activated
vascular
endothelium
may
contribute
acutely
the
initiation
painful
vaso-occlusive
crises
chronically
endothelial
damage
in
SCD.
RBCs
adhere
through
several
mechanisms.
In
this
study,
using
whole
from
17
people
heterozygous
SCD
(HbS
variant)
55
homozygous
(HbSS)
analyzed
an
vitro
microfluidic
assay,
we
present
evidence
for
immobilized
recombinant
intercellular
molecule
1
(ICAM-1).
We
show
that
RBC
ICAM-1
hemolysis
vivo,
marked
by
elevated
lactate
dehydrogenase
levels,
reticulocytosis,
lower
fetal
hemoglobin
levels.
Further,
correlates
a
history
intracardiac
or
intrapulmonary
right-to-left
shunts.
Studies
potential
ligands
on
membranes
revealed
RBC–ICAM-1
interactions
were
mediated
fibrinogen
bound
membrane.
describe,
first
time,
rolling
behavior
under
high
shear
rates.
Our
results
suggest
firm
most
likely
occurs
postcapillary
venules
at
low
physiological
rates,
facilitated
initial
regions
(eg,
capillaries).
Inhibition
constitute
novel
therapeutic
target
