Heliyon,
Journal Year:
2024,
Volume and Issue:
10(16), P. e35907 - e35907
Published: Aug. 1, 2024
BackgroundThis
study
aimed
to
evaluate
morphological,
chemical
and
biocompatible
properties
of
nanohydroxyapatite
(N-HA)
synthesized
from
eggshells
dual-doped
with
Si4+
Zn2+.MethodsIn
the
current
study,
N-HA
was
chicken
using
wet
precipitation
method
doped
Zn2+.
The
physical
assessment
carried
out
field
emission
scanning
electron
microscopy
(FE-SEM),
energy
dispersive
X-ray
(EDX)
analysis,
diffraction
(XRD)
analysis.
Crystal
size
calculated
Scherrer
equation.
Cytotoxicity
studied
in
vitro
MTT
(3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium
Bromide)
cytotoxicity
assay.
optical
density
(OD)
each
well
obtained
recorded
at
570
nm
for
24
h
(t1),
48
(t2),
72
(t3),
5
days
(t4)
a
microplate
reader.ResultsThe
results
Si–Zn-doped
HA
showed
high
specific
surface
area
an
irregular
nano-sized
spherical
particle
structure.
atomic
percentage
provided
ratio
calcium
phosphate;
non-doped
HA,
Ca/P
1.6,
but
where
Zn+2
Ca
Si
+
replaced
4
substituted
P,
(Ca
Zn)/(P
Si)
1.76.
average
crystal
46
nm,
while
it
61
nm.
both
samples
were
non-toxic
statistically
significantly
less
viable
than
control
group
After
days,
mean
cell
viability
(79.17
±
2.18)
higher
that
(76.26
1.71)
(P
=
0.091).ConclusionsThe
assay
is
biocompatible.
In
addition,
characteristic
physiochemical
large
interconnected
porosity.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Oct. 31, 2023
Infected
bone
defects
are
a
major
challenge
in
orthopedic
treatment.
Native
tissue
possesses
an
endogenous
electroactive
interface
that
induces
stem
cell
differentiation
and
inhibits
bacterial
adhesion
activity.
However,
traditional
substitutes
have
difficulty
reconstructing
the
electrical
environment
of
bone.
In
this
study,
we
develop
self-promoted
mineralized
scaffold
(sp-EMS)
generates
weak
currents
via
spontaneous
electrochemical
reactions
to
activate
voltage-gated
Ca2+
channels,
enhance
adenosine
triphosphate-induced
actin
remodeling,
ultimately
achieve
osteogenic
mesenchymal
cells
by
activating
BMP2/Smad5
pathway.
Furthermore,
show
provided
sp-EMS
activity
products
concomitantly
generated
reactive
oxygen
species.
We
find
antibacterial
dual
functions
depend
on
its
self-promoting
stimulation.
demonstrate
vivo,
achieves
complete
or
nearly
situ
infected
healing,
from
rat
calvarial
defect
model
with
single
infection,
rabbit
open
alveolar
beagle
dog
vertical
complex
oral
microenvironment.
This
translational
study
demonstrates
graft
presents
promising
therapeutic
platform
for
repair.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(23)
Published: Jan. 7, 2024
Abstract
The
incidence
of
large
bone
and
articular
cartilage
defects
caused
by
traumatic
injury
is
increasing
worldwide;
the
tissue
regeneration
process
for
these
injuries
lengthy
due
to
limited
self‐healing
ability.
Endogenous
bioelectrical
phenomenon
has
been
well
recognized
play
an
important
role
in
homeostasis
regeneration.
Studies
have
reported
that
electrical
stimulation
(ES)
can
effectively
regulate
various
biological
processes
holds
promise
as
external
intervention
enhance
synthesis
extracellular
matrix,
thereby
accelerating
Hence,
electroactive
biomaterials
considered
a
biomimetic
approach
ensure
functional
recovery
integrating
physiological
signals,
including
electrical,
biochemical,
mechanical
signals.
This
review
will
discuss
endogenous
bioelectricity
tissue,
effects
ES
on
cellular
behaviors.
Then,
recent
advances
materials
their
applications
are
systematically
overviewed,
with
focus
advantages
disadvantages
repair
performances
modulation
cell
fate.
Finally,
significance
mimicking
electrophysiological
microenvironment
target
emphasized
future
development
challenges
strategies
proposed.
Animal Models and Experimental Medicine,
Journal Year:
2023,
Volume and Issue:
6(2), P. 120 - 130
Published: March 1, 2023
Abstract
Understanding
the
bioelectrical
properties
of
bone
tissue
is
key
to
developing
new
treatment
strategies
for
diseases
and
injuries,
as
well
improving
design
fabrication
scaffold
implants
engineering.
The
can
be
attributed
interaction
its
various
cell
lineages
(osteocyte,
osteoblast
osteoclast)
with
surrounding
extracellular
matrix,
in
presence
biomechanical
stimuli
arising
from
routine
physical
activities;
best
described
a
combination
overlap
dielectric,
piezoelectric,
pyroelectric
ferroelectric
properties,
together
streaming
potential
electro‐osmosis.
There
close
interdependence
electroactive
electrosensitive
components
tissue,
including
membrane
potential,
voltage‐gated
ion
channels,
intracellular
signaling
pathways,
surface
receptors,
matrix
such
collagen,
hydroxyapatite,
proteoglycans
glycosaminoglycans.
It
remarkably
complex
web
interactive
cross‐talk
between
organic
non‐organic
that
define
electrophysiological
which
turn
exerts
profound
influence
on
metabolism,
homeostasis
regeneration
health
disease.
This
has
spurred
increasing
interest
application
scaffolds
engineering,
recapitulate
natural
microenvironment
healthy
facilitate
defect
repair.
Composites Science and Technology,
Journal Year:
2023,
Volume and Issue:
243, P. 110263 - 110263
Published: Sept. 20, 2023
Replicating
the
natural
cellular
environment
is
a
critical
strategy
when
employing
biomaterials
to
enhance
tissue
regeneration.
However,
effectively
controlling
physical
cues,
including
electrical
and
mechanical
stimuli,
in
extracellular
microenvironment
promote
regeneration,
remains
challenging
endeavor.
This
study
presents
technological
utilization
of
magnetoelectric
(ME)
composites,
capable
delivering
stimuli
through
remote
activation
using
magnetic
field,
for
applications
bone-related
engineering.
Poly(vinylidene
fluoride-co-trifluoroethylene)
scaffolds
incorporating
two
types
magnetostrictive
particles,
namely
Terfenol-D
(TD)
microparticles
CoFe2O4
(CFO)
nanoparticles,
were
used
investigate
impact
mechano-electrical
on
preosteoblast
cells.
The
results
demonstrate
that
such
are
applied
custom-made
bioreactor,
both
proliferation
rate
mineralization
increase.
Such
outcomes
dependent
specific
particles
incorporated
composite.
These
findings
underscore
significance
designing
properties
ME
active
achieve
successful
bone
Thus,
presented
emulate
microenvironment,
enabling
precise,
controlled,
effective
regenerative
therapies
Journal of Biomaterials Applications,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 11, 2025
In
the
repair
of
large
bone
defects,
loss
periosteum
can
result
in
diminished
osteoinductive
activity,
nonunion,
and
incomplete
regeneration
structure,
ultimately
compromising
efficiency
regeneration.
Therefore,
research
development
tissue-engineered
which
replace
function
has
become
focus
current
research.
The
functionalized
electrospinning
is
expected
to
mimic
natural
enhance
processes
more
effectively.
This
review
explores
construction
strategies
for
electrospun
from
following
perspectives:
ⅰ)
bioactive
factor
modification
(bone
morphogenetic
protein-2
(BMP-2),
vascular
endothelial
growth
(VEGF)
etc.),
ⅱ)
inorganic
compound
modification,
ⅲ)
drug
ⅳ)
artificial
response
physical
stimuli.
Furthermore,
through
electrospinning,
conjunction
with
other
strategies,
also
analyzed.
Finally,
challenges
prospects
are
discussed.
Cells,
Journal Year:
2025,
Volume and Issue:
14(2), P. 145 - 145
Published: Jan. 20, 2025
Maxillofacial
bone
defects
can
have
a
profound
impact
on
both
facial
function
and
aesthetics.
While
various
biomaterial
scaffolds
shown
promise
in
addressing
these
challenges,
regenerating
this
region
remains
complex
due
to
its
irregular
shape,
intricate
structure,
differing
cellular
origins
compared
other
bones
the
human
body.
Moreover,
significant
variable
mechanical
loads
placed
maxillofacial
add
further
complexity,
especially
cases
of
difficult-to-treat
medical
conditions.
This
review
provides
brief
overview
medication-related
osteonecrosis
jaw
(MRONJ),
highlighting
medication-induced
adverse
reactions
associated
clinical
challenges
treating
condition.
The
purpose
manuscript
is
emphasize
role
biotechnology
tissue
engineering
technologies
therapy.
By
using
scaffold
materials
biofactors
combination
with
autologous
cells,
innovative
solutions
are
explored
for
repair
damaged
bones.
ongoing
search
effective
that
address
improve
vitro
preparation
subsequent
regeneration
critical.
primary
spotlight
current
research
trends
novel
approaches
area.
