Organelle Targeting Self-Assembled Fluorescent Probe for Anticancer Treatment DOI Creative Commons
Md. Sajid Hasan,

Sangpil Kim,

Chaelyeong Lim

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(7), P. 138 - 138

Published: July 11, 2024

Organic fluorescent probes have attracted attention for bioimaging due to their advantages, including high sensitivity, biocompatibility, and multi-functionality. However, some limitations related low signal-to-background ratio false positive negative signals make them difficult in situ target detection. Recently, organelle targeting self-assembled been studied meet this demand. Most of the dye molecules suffer from a quenching effect, but, specifically, dyes like Pyrene, Near-Infrared (NIR), Nitrobenzoxadiazole (NBD), Fluorescein isothiocyanate (FITC), Naphthalenediimides (NDI), Aggregation induced emission (AIE) show unique characteristics when they undergo self-assembly or aggregation. Therefore, review, we classified according type provided an overview organelle-targeting strategy with emphasis on construction nanostructures within complex cellular environments. Results demonstrated that effectively localized inside organelles (mitochondria, lysosome, golgi body) form various possess bio-functionality long retention time, membrane disruption/ROS generation/enzyme activity suppression ability, enhanced photodynamic properties anticancer treatment. Furthermore, systematically discussed challenges remain be resolved performance these mentioned future directions design molecules.

Language: Английский

Host–Guest Adduct as a Stimuli‐Responsive Prodrug: Enzyme‐Triggered Self‐Assembly Process of a Short Peptide Within Mitochondria to Induce Cell Apoptosis DOI
Sandip Sarkar,

Atin Chatterjee,

Dohyun Kim

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 14(4)

Published: Nov. 6, 2024

To address the issue of nonspecific biodistribution a chemotherapeutic drug, stable [2]pseudorotaxane complexes (PK@CAOPP and PR@CAOPP) are used to demonstrate proof concept. Cationic -PPh3 + moiety in CAOPP allows specific localization PK@CAOPP/ PR@CAOPP mitochondrial membrane (MM). Electrostatic interaction between cationic LysinePK or ArgininePR negatively charged phosphoesterCAOPP functionality favours strong adduct formation. The ALP-induced hydrolytic cleavage phosphoester cancer cells triggers dephosphorylation releases PK/ PR from PK@CAOPP/PR@CAOPP. PK PR, derived Phe-Phe dipeptide, formed fibril-like molecular aggregates MM induce dysfunction, depolarization, ROS generation apoptotic MCF7 cell death. Such phenomena were not observed ALP-negative HEK293 normal cells. These propositions confirmed through control studies using NBDK PE, other guest molecules. Smaller size inclusion short peptides (PK PR) within hydrophobic interior CAOPP, attributed their stability blood serum. Thus, we have demonstrated use supramolecular adducts as potential therapeutic option for treating without affecting healthy efficacy was also established with an in-vivo tumour xenograft model Balb/c nude mice.

Language: Английский

Citations

5
Living Cell-Mediated Self-Assembly: From Monomer Design and Morphology Regulation to Biomedical Applications DOI
Chengfei Liu,

Haonan Ma,

Shaohui Yuan

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

The self-assembly of molecules into highly ordered architectures is a ubiquitous and natural process, wherein spontaneously organize large structures to perform diverse functions. Drawing inspiration from the formation nanostructures, cell-mediated has been developed create functional assemblies both inside outside living cells. These techniques have employed regulate cellular world by leveraging dynamic intracellular extracellular microenvironment. This review highlights recent advances future trends in self-assembly, ranging their cytocompatible monomer designs, synthetic strategies, morphological control applications. assembly behaviors are also discussed based on dimensionality self-assembled morphologies zero three dimensions. Finally, this explores its promising potential for biomedical applications, clarifying relationship between initial regulation therapeutic effects subsequent artificial assemblies. Through rationally designing molecular precisely controlling morphologies, cell mediated would provide an innovative platform executing biological

Language: Английский

Citations

0
Cancer Specific CAIX‐Targeting Supramolecular Lysosome‐Targeting Chimeras (Supra‐LYTAC) for Targeted Protein Degradation DOI Creative Commons
Do‐Hyun Kim,

Gyeongseok Yang,

Chaelyeong Lim

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Recently, targeted protein degradation (TPD) strategies have emerged as a promising solution to tackle undruggable proteins. While most TPD target intracellular proteins, limited options exist for targeting extracellular or membrane Herein, cancer specific carbonic anhydrase IX (CAIX)-targeting supramolecular nanofibrous lysosome-targeting chimeras (Supra-LYTAC) is reported. Two self-assembling amphiphilic peptides are synthesized: one that interacts with the of interest (POI), and another mediates lysosomal endocytosis by cancer-specific enzyme. Notably, these two co-assemble into nanofibers capable cells in spatiotemporal manner. Through dynamic multivalent binding, ternary complex form (supramolecular chimeric nanostructure; CAIX-nanofiber-POI), which undergoes internalization lysosomes where POI degraded through catalytic activity. This study demonstrates potential approaches expand scope LYTAC technology, offering new opportunities designing future.

Language: Английский

Citations

0
Nanoarchitectonics for Biomedical Research: Post‐Nanotechnology Materials Approach for Bio‐Active Application DOI Creative Commons
Katsuhiko Ariga

Advanced NanoBiomed Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Nanoarchitectonics, as a post‐nanotechnology concept, represents methodology for the construction of functional materials employing atoms, molecules, and nanomaterials essential components. The overarching objective nanoarchitectonics is to develop systems comprising multiple units assembled in hierarchical manner, observed biological systems. Nevertheless, such challenging endeavor. It would be prudent, therefore, initially focus on development that interact with complex structures living organisms. Accordingly, this review article addresses topic nanoarchitecture it pertains biomedical applications. This examines current trends research presents examples studies support concept its applications fields. presented are follows: i) molecular developments, which mainly based design assembly; ii) material examples, using components; iii) porous materials, will summarized under heading pore‐engineered due their special structure. Finally, provides an overview these discusses future prospects.

Language: Английский

Citations

2
Enzyme-instructed intramitochondrial polymerization for enhanced anticancer treatment without the development of drug-resistance DOI

Sangpil Kim,

Yeji Lee,

Min‐Seok Seu

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 373, P. 189 - 200

Published: July 16, 2024

Language: Английский

Citations

1
Folic Acid-Functionalized β-Cyclodextrin for Delivery of Organelle-Targeted Peptide Chemotherapeutics in Cancer DOI

Hae Won Ok,

Seongeon Jin,

Gaeun Park

et al.

Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: 21(9), P. 4498 - 4509

Published: July 29, 2024

Recent emphasis on the design of drug delivery systems typically involves effective transport a pharmaceutical substance to disease site with desired therapeutic efficacy and minimal cytotoxicity. Organelle-targeted peptides have become an integral part designing important class prodrug/prodrug assemblies for new supramolecular therapeutics owing their favorable biocompatibility, synthetic ease, tunability aggregation behavior, functionalization site-specificity. However, it is still limited due low selectivity. We designed folic acid-functionalized β-cyclodextrin (FA-CD) as platform specific selective organelle-targeted (such microtubule, lysosome, mitochondria) peptide chemotherapeutics folate receptor (FR) overexpressing cancer cell lines. Low toxicity was found FA-CD inclusion complex in FR-negative normal cells, but superior inhibition tumor growth no vivo xenograft model.

Language: Английский

Citations

1
Artificial Polymerizations in Living Organisms for Biomedical Applications DOI
Yun Chen,

Brynne Shu Ni Tan,

Yu Cheng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 1, 2024

Abstract Within living organisms, numerous nanomachines are constantly involved in complex polymerization processes, generating a diverse array of biomacromolecules for maintaining biological activities. Transporting artificial polymerizations from lab settings into contexts has expanded opportunities understanding and managing events, creating novel cellular compartments, introducing new functionalities. This review summarizes the recent advancements polymerizations, including those responding to external stimuli, internal environmental factors, that polymerize spontaneously. More importantly, cutting‐edge biomedical application scenarios polymerization, notably safeguarding cells, modulating improving diagnostic performance, facilitating therapeutic efficacy highlighted. Finally, this outlines key challenges technological obstacles remain as well offers insights potential directions advancing their practical applications clinical trials.

Language: Английский

Citations

1
Artificial Polymerizations in Living Organisms for Biomedical Applications DOI
Yun Chen,

Brynne Shu Ni Tan,

Yu Cheng

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(43)

Published: Aug. 1, 2024

Abstract Within living organisms, numerous nanomachines are constantly involved in complex polymerization processes, generating a diverse array of biomacromolecules for maintaining biological activities. Transporting artificial polymerizations from lab settings into contexts has expanded opportunities understanding and managing events, creating novel cellular compartments, introducing new functionalities. This review summarizes the recent advancements polymerizations, including those responding to external stimuli, internal environmental factors, that polymerize spontaneously. More importantly, cutting‐edge biomedical application scenarios polymerization, notably safeguarding cells, modulating improving diagnostic performance, facilitating therapeutic efficacy highlighted. Finally, this outlines key challenges technological obstacles remain as well offers insights potential directions advancing their practical applications clinical trials.

Language: Английский

Citations

1
Organelle Targeting Self-Assembled Fluorescent Probe for Anticancer Treatment DOI Creative Commons
Md. Sajid Hasan,

Sangpil Kim,

Chaelyeong Lim

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(7), P. 138 - 138

Published: July 11, 2024

Organic fluorescent probes have attracted attention for bioimaging due to their advantages, including high sensitivity, biocompatibility, and multi-functionality. However, some limitations related low signal-to-background ratio false positive negative signals make them difficult in situ target detection. Recently, organelle targeting self-assembled been studied meet this demand. Most of the dye molecules suffer from a quenching effect, but, specifically, dyes like Pyrene, Near-Infrared (NIR), Nitrobenzoxadiazole (NBD), Fluorescein isothiocyanate (FITC), Naphthalenediimides (NDI), Aggregation induced emission (AIE) show unique characteristics when they undergo self-assembly or aggregation. Therefore, review, we classified according type provided an overview organelle-targeting strategy with emphasis on construction nanostructures within complex cellular environments. Results demonstrated that effectively localized inside organelles (mitochondria, lysosome, golgi body) form various possess bio-functionality long retention time, membrane disruption/ROS generation/enzyme activity suppression ability, enhanced photodynamic properties anticancer treatment. Furthermore, systematically discussed challenges remain be resolved performance these mentioned future directions design molecules.

Language: Английский

Citations

0