DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(36)

Published: June 14, 2024

Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio-applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria-targeted tumor treatment. We first highlight engineering mitochondria-localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti-tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

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Chemical Engineering of DNAzyme for Effective Biosensing and Gene Therapy

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

RNA-cleaving DNAzymes are in vitro selected functional nucleic acids with inherent catalytic activities. Due to their unique properties, such as high specificity, substrate cleavage capability, and programmability, have emerged powerful tools the fields of analytical chemistry, chemical biology, biomedicine. Nevertheless, biological applications still impeded by several challenges, structural instability, compromised activity environments lack spatiotemporal control designs, which may result false-positive signals, limited efficacy or non-specific activation associated side effects. To address these various strategies been explored regulate DNAzyme through modifications, enhancing stability, selectivity, functionality, thereby positioning them ideal candidates for applications. In this review, a comprehensive overview chemically modified is provided, discussing modification effects modifications on DNAzymes. Specific examples use biosensing gene therapy also presented discussed. Finally, current challenges field addressed offer perspectives potential direction

Language: Английский

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Programmable DNA‐Based Nanodevices for Next‐Generation Clinical and Healthcare Applications

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Deoxyribonucleic acid (DNA) nanotechnology has brought an unparalleled set of possibilities for self‐assembled structures emerging as independent branch synthetic biology. The field science uses the molecular properties DNA to build nanoparticles and nanodevices that have potential bring breakthroughs in medical science. On one hand, their biocompatibility, precision, ease, programmability make them ideal choice drug delivery healthcare. other, lack proper biodistribution profiles, stability inside system, enzymatic cleavage, immune recognition, translational barriers are some hurdles it faces. Many recent technological advancements progress tackle these challenges, while already been used. These tools technologies need be understood studied successful transition intelligent nanostructures (DNs) healthcare applications. This review thus, highlights challenges being faced by DNs Additionally, provides overview trends using devices disease detection remission finally talks about future scope opportunities effective from bench bedside.

Language: Английский

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Cell-free DNA-scavenging nano/microsystems for immunotherapy

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113609 - 113609

Published: March 1, 2025

Language: Английский

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DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(36)

Published: June 14, 2024

Abstract Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio‐applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria‐targeted tumor treatment. We first highlight engineering mitochondria‐localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti‐tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

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Exploring Framework Nucleic Acids: A Perspective on Their Cellular Applications

JACS Au, Journal Year: 2024, Volume and Issue: 4(11), P. 4110 - 4128

Published: Oct. 22, 2024

Cells are fundamental units of life. The coordination cellular functions and behaviors relies on a cascade molecular networks. Technologies that enable exploration manipulation specific events in living cells with high spatiotemporal precision would be critical for pathological study, disease diagnosis, treatment. Framework nucleic acids (FNAs) represent novel class acid materials characterized by their monodisperse rigid nanostructure. Leveraging exceptional programmability, convenient modification property, predictable atomic-level architecture, FNAs have attracted significant attention diverse applications such as cell recognition, imaging, manipulation, therapeutic interventions. In this perspective, we will discuss the utilization systems while critically assessing opportunities challenges presented burgeoning field.

Language: Английский

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Multi-DNAzymes cascade reaction mediated aptasensors for OTA detection based on the integration of autocatalytic Mg2+-dependent DNAzyme cleavage and entropy-driven circuit

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 289, P. 138896 - 138896

Published: Dec. 18, 2024

Language: Английский

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