DNA‐Assisted Separation of Nanoparticles DOI Open Access
Ying Sun, Yuchuan Liu, Daqian Song

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

DNA, as natural polymers, not only serves the genetic material but also shows great potential assistants for efficient and targeted nanoparticle separation. This is due to its high programmability, excellent biocompatibility, stability effectively binding with various types of nanoparticles or bioparticles, while modifying physical properties target particles such charge, mass size. Researchers have achieved remarkable efficiency in separating by precisely designing DNA sequences their interactions particles. achievement primarily relies on differences medium modulated DNA. In this minireview, diverse strategies are overviewed using assist separation, applications these separation highlighted. Finally, current challenges prospects DNA-assisted discussed.

Language: Английский

Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy DOI
Bo Wu, Jian Zhao, Fangzhi Yu

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective ncRNA (e.g., microRNA (miRNA)) mitochondria via an organelle-specific DNA assembly strategy. The target miRNA-initiated hairpins is inhibited by the block toehold-mediated strand displacement reaction can be exclusively activated mitochondria-encoded ribosomal RNA (rRNA) hybridization chain reaction, enabling spatial control over miRNA imaging. We demonstrate that conditionally controlled technology minimization nonspecific activation and thus improves precision detection. In addition, strategy adaptable to visualizing other ncRNAs such as long mitochondria, highlighting universality approach. Overall, this work provides useful tool investigating organelle-located RNA.

Language: Английский

Citations

0
Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies DOI Creative Commons
Wanchong He,

Siyu Dong,

Qinghua Zeng

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1025 - 1025

Published: Feb. 24, 2025

Mitochondria, as vital organelles, play a central role in subcellular research and biomedical innovation. Although functional nucleic acid (FNA) nanostructures have witnessed remarkable progress across numerous biological applications, strategies specifically tailored to target mitochondria for molecular imaging therapeutic interventions remain scarce. This review delves into the latest advancements leveraging FNA mitochondria-specific cancer therapy. Initially, we explore creation of FNA-based biosensors localized mitochondria, enabling real-time detection visualization critical molecules essential mitochondrial function. Subsequently, examine developments aimed at mitochondrial-targeted treatments, including modular nanodevices precise delivery agents programmable disrupting processes. Emphasis is placed on elucidating chemical principles underlying design mitochondrial-specific nanotechnology diverse uses. Lastly, address unresolved challenges outline prospective directions, with goal advancing field encouraging sophisticated tools both academic inquiry clinical applications centered mitochondria.

Language: Английский

Citations

0
Multiple roles of mitochondria in tumorigenesis and treatment: from mechanistic insights to emerging therapeutic strategies DOI Creative Commons
Min Zhang, Yuchen Yang, Jiacheng Wu

et al.

ONCOLOGIE, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

0
DNA‐Encoded Fluorescence Signals for Imaging Analysis DOI Open Access
Xiaowen Cao,

Wenhao Fu,

X Li

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Fluorescence imaging has been a powerful technique for the visualization of interest biomolecules. It is widely used in basic biological research, clinical medicine, and other fields. However, fluorescence signals are often too weak detecting with simple devices or complex environments. Besides, limited to about four six dyes, restricting spectral overlap organic fluorophores. DNA nanotechnology including structured dynamic emerges as promising material encode fluorophores, holding great potential improve properties signals. Substantial progresses have achieved DNA-encoded signals, exhibiting novel characteristics applications. This review summarizes various encoding strategies their performance analysis. In this review, different methods impacts on reported, such brightness, photostability, kinetics, multiplexing. application reviewed. Finally, solutions address current challenges suggested, encouraging future development area.

Language: Английский

Citations

0
Molecular engineering strategies for fabricating type-I mitochondria-targeted aggregation-induced emission photosensitizers for apoptosis-ferroptosis synergistically boosting photodynamic therapy DOI
Shihua Wang, Yeting Qiu, Lichao Yu

et al.

Journal of Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 694, P. 137680 - 137680

Published: April 23, 2025

Language: Английский

Citations

0
Engineering Acidic Deoxyribozyme with Strongly Cooperative H+ Binding for Subcellular Imaging DOI
Jia Pan,

Q. W. Meng,

Qiang Zhang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Engineering deoxyribozymes under extreme conditions will expand their repertoires for unique applications. Although all known deoxyribozyme-based biosensors were derived to function or near physiological conditions, no prior study has been conducted purposely build them absolutely atypical conditions. Here, we reported the design and characterization of an acidic RNA-cleaving deoxyribozyme, termed ET46, that displays strongly cooperative H+ binding performing catalysis at pH, yielding a Hill coefficient 5.4. This feature cooperativity significantly improves responsiveness ET46 small pH variations within range 4.5-5.5. We further exploited pH-sensitive properties track changes on uptake localization in lysosomes. Finally, developed lysosomal ATP-activated fluorescence-signaling aptazyme biosensor (named FabATP) using ATP-binding DNA aptamer. To demonstrate its ability lysosomes, applied FabATP live subcellular fluorescence imaging ATP fluctuations upon stimulations.

Language: Английский

Citations

0
Subcellular Compartment‐Specific Amplified Imaging of Metal Ions via Ribosomal RNA‐Regulated DNAzyme Sensors DOI

Deyu Yi,

Lele Li, Mengyuan Li

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Abstract Although DNAzyme sensors have been widely developed for imaging metal ions, their application in specific subcellular compartments remains challenging due to low spatial controllability. Here we present a locally activatable, DNAzyme‐based sensing technology that enables compartment‐specific of ions through ribosomal RNA (rRNA) regulated signal amplification. The system leverages subcellularly encoded rRNA activate sensors, and further drives amplification via multiple turnover cleavage molecular beacons, significantly enhance sensitivity precision metal‐ion organelles (e.g. mitochondria) or membraneless cytosol). Furthermore, demonstrate the allows situ monitoring dynamics mitochondrial Zn 2+ during ischemia drug intervention. This study expands toolbox investigating role disease processes.

Language: Английский

Citations

3
Subcellular Compartment‐Specific Amplified Imaging of Metal Ions via Ribosomal RNA‐Regulated DNAzyme Sensors DOI Open Access

Deyu Yi,

Lele Li, Mengyuan Li

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Although DNAzyme sensors have been widely developed for imaging metal ions, their application in specific subcellular compartments remains challenging due to low spatial controllability. Here we present a locally activatable, DNAzyme-based sensing technology that enables compartment-specific of ions through ribosomal RNA (rRNA) regulated signal amplification. The system leverages subcellularly encoded rRNA activate sensors, and further drives amplification via multiple turnover cleavage molecular beacons, significantly enhance sensitivity precision metal-ion organelles (e.g. mitochondria) or membraneless cytosol). Furthermore, demonstrate the allows situ monitoring dynamics mitochondrial Zn

Language: Английский

Citations

2
Light-Triggered Plasmonic DNAzyme Walker Enables Precise Subcellular Molecular Imaging with Reduced Off-Mitochondria Signal Leakage DOI

Xiao-Zhe Pang,

Haiming Li, Xiaohan Xu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(42), P. 16971 - 16977

Published: Oct. 11, 2024

The development of highly sensitive and precise imaging techniques capable visualizing crucial molecules at the subcellular level is essential for elucidating mitochondrial functions uncovering novel mechanisms in biological processes. However, traditional molecular strategies are still limited by off-mitochondria signal leakage because "always-active" sensing mode. To address this limitation, we have developed a light-triggered activation sequence activated plasmonic DNAzyme walker (PDW) accurate combination an organelle localized strategy, upconversion nanotechnology, plasmon enhanced fluorescence (PEF) technique. Exploiting advantage light enables control over when where to activate probe's function, effectively reducing as validated dynamic monitoring changes signals during entry process. Furthermore, leveraging PEF capability triangular gold nanoprisms (Au NPRs), intensity can be approximately 11.9 times, ensuring imaging.

Language: Английский

Citations

1
Spatially Controlled MicroRNA Imaging in Mitochondria via Enzymatic Activation of Hybridization Chain Reaction DOI Open Access

Kaining Dai,

Jian Zhao, Lele Li

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Live‐cell imaging of RNA in specific subcellular compartments is essential for elucidating the rich repertoire cellular functions, but it has been limited by a lack simple, precisely controlled methods. Here such an approach presented via combination hybridization chain reaction and spatially restricted enzymatic activation with organelle‐targeted delivery. The system can localize engineered DNA hairpins mitochondria, where target RNA‐initiated events selectively activated enzyme, enabling amplified high precision. It demonstrated that compatible live cell visualization enables regulatable microRNA mitochondria. Since situ signal amplification enzyme eliminates need genetically encoded protein overexpression, envisioned this simple platform will be broadly applicable precise resolution variety biological processes.

Language: Английский

Citations

1