Journal of the American Chemical Society,
Journal Year:
2020,
Volume and Issue:
142(31), P. 13350 - 13355
Published: July 24, 2020
We
report
the
development
of
a
new
strategy
for
chemical
analysis
live
cells
based
on
protein
spherical
nucleic
acids
(ProSNAs).
The
ProSNA
architecture
enables
analyte
detection
via
highly
programmable
acid
shell
or
functional
core.
As
proof-of-concept,
we
use
an
i-motif
as
recognition
element
to
probe
pH
in
living
cells.
By
interfacing
with
forced-intercalation
readout,
introduce
quencher-free
approach
that
is
resistant
false-positive
signals,
overcoming
limitations
associated
conventional
fluorophore/quencher-based
gold
NanoFlares.
Using
glucose
oxidase
core,
show
activity-based,
amplified
sensing
glucose.
This
enzymatic
system
affords
greater
than
100-fold
fluorescence
turn
buffer,
selective
presence
close
analogs
(i.e.,
glucose-6-phosphate),
and
can
detect
above
threshold
concentration
∼5
μM,
which
study
relative
changes
intracellular
concentrations.
Chemical Society Reviews,
Journal Year:
2022,
Volume and Issue:
51(5), P. 1795 - 1835
Published: Jan. 1, 2022
The
discovery
of
a
near-infrared
(NIR,
650-900
nm)
fluorescent
chromophore
hemicyanine
dye
with
high
structural
tailorability
is
great
significance
in
the
field
detection,
bioimaging,
and
medical
therapeutic
applications.
It
exhibits
many
outstanding
advantages
including
absorption
emission
NIR
region,
tunable
spectral
properties,
photostability
as
well
large
Stokes
shift.
These
properties
are
superior
to
those
conventional
fluorogens,
such
coumarin,
fluorescein,
naphthalimides,
rhodamine,
cyanine.
Researchers
have
made
remarkable
progress
developing
activity-based
multifunctional
probes
based
on
skeletons
for
monitoring
vital
biomolecules
living
systems
through
output
fluorescence/photoacoustic
signals,
integration
diagnosis
treatment
diseases
using
chemotherapy
or
photothermal/photodynamic
therapy
combination
therapy.
achievements
prompted
researchers
develop
more
smart
fluorogen
template.
In
this
review,
we
begin
by
describing
brief
history
dyes,
synthetic
approaches,
design
strategies
functional
probes.
Then,
selected
hemicyanine-based
that
can
detect
ions,
small
biomolecules,
overexpressed
enzymes
diagnostic
reagents
systematically
highlighted.
Finally,
potential
drawbacks
outlook
future
investigation
clinical
medicine
transformation
activatable
also
discussed.
Angewandte Chemie International Edition,
Journal Year:
2020,
Volume and Issue:
60(32), P. 17268 - 17289
Published: Sept. 17, 2020
Abstract
Near‐infrared
(NIR)
activatable
fluorescent
probes
have
been
considered
to
be
the
effective
edge
tools
for
investigation
of
cell
biology
and
disease
diagnosis
because
their
outstanding
advantages.
Related
genes
involved
in
tumor
genesis
progression
regulate
overexpression
certain
enzymes.
Owing
distinctive
characteristics
quick
reaction
time
favorable
pharmacokinetics,
enzyme‐reactive
NIR
optical
shown
great
potential
tumorigenesis
image‐guided
intraoperative
surgeries
with
high
signal‐to‐noise
ratios.
In
this
review,
we
mainly
summarize
latest
advancements
from
design
strategy
biomedical
application.
Specifically,
some
challenges
prospects
field
are
presented
at
end
which
will
beneficial
innovatively
construct
new
multifunctional
actively
promote
clinical
transformation
future.
International Journal of Biological Macromolecules,
Journal Year:
2022,
Volume and Issue:
206, P. 115 - 147
Published: Feb. 26, 2022
Thanks
to
their
unique
attributes,
such
as
good
sensitivity,
selectivity,
high
surface-to-volume
ratio,
and
versatile
optical
electronic
properties,
fluorescent-based
bioprobes
have
been
used
create
highly
sensitive
nanobiosensors
detect
various
biological
chemical
agents.
These
sensors
are
superior
other
analytical
instrumentation
techniques
like
gas
chromatography,
high-performance
liquid
capillary
electrophoresis
for
being
biodegradable,
eco-friendly,
more
economical,
operational,
cost-effective.
Moreover,
several
reports
also
highlighted
application
in
the
early
detection
of
biomarkers
associated
with
drug-induced
organ
damage
liver,
kidney,
or
lungs.
In
present
work,
we
comprehensively
overviewed
electrochemical
that
employ
nanomaterials
(nanoparticles/colloids
quantum
dots,
carbon
nanoscaled
metal-organic
frameworks,
etc.)
a
variety
macromolecules
based
on
fluorescent
emission
spectra.
addition,
most
important
mechanisms
methods
sense
amino
acids,
protein,
peptides,
enzymes,
carbohydrates,
neurotransmitters,
nucleic
vitamins,
ions,
metals,
electrolytes,
blood
gases,
drugs
(i.e.,
anti-inflammatory
agents
antibiotics),
toxins,
alkaloids,
antioxidants,
cancer
biomarkers,
urinary
metabolites
urea,
uric
acid,
creatinine),
pathogenic
microorganisms
were
outlined
compared
terms
selectivity
sensitivity.
Altogether,
small
dimensions
capability
these
nanosensors
sensitive,
label-free,
real-time
sensing
chemical,
biological,
pharmaceutical
could
be
array-based
screening
in-vitro
in-vivo
diagnostics.
Although
nanoprobes
widely
applied
determining
macromolecules,
unfortunately,
they
many
challenges
limitations.
Efforts
must
made
minimize
limitations
utilizing
an
emphasis
commercial
developments.
We
believe
current
review
can
foster
wider
incorporation
nanomedicine
will
particular
interest
researchers
working
fluorescence
technology,
material
chemistry,
coordination
polymers,
related
research
areas.
Organic & Biomolecular Chemistry,
Journal Year:
2020,
Volume and Issue:
18(30), P. 5747 - 5763
Published: Jan. 1, 2020
Guidelines
based
on
photophysical
tuning,
reactivity,
isomerization,
and
energy
transfer
for
rational
design
of
synthetic
fluorescent
probes
biological
systems.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(15), P. 14347 - 14405
Published: July 24, 2023
Light
has
profoundly
impacted
modern
medicine
and
healthcare,
with
numerous
luminescent
agents
imaging
techniques
currently
being
used
to
assess
health
treat
diseases.
As
an
emerging
concept
in
luminescence,
aggregation-induced
emission
(AIE)
shown
great
potential
biological
applications
due
its
advantages
terms
of
brightness,
biocompatibility,
photostability,
positive
correlation
concentration.
This
review
provides
a
comprehensive
summary
AIE
luminogens
applied
structure
dynamic
physiological
processes,
disease
diagnosis
treatment,
detection
monitoring
specific
analytes,
followed
by
representative
works.
Discussions
on
critical
issues
perspectives
future
directions
are
also
included.
aims
stimulate
the
interest
researchers
from
different
fields,
including
chemistry,
biology,
materials
science,
medicine,
etc.,
thus
promoting
development
fields
life
health.
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(7), P. 2322 - 2357
Published: Jan. 1, 2023
Typical
PeT-based
fluorescent
probes
are
multi-component
systems
where
a
fluorophore
is
connected
to
recognition/activating
group
by
an
unconjugated
linker.
powerful
tools
for
cell
imaging
and
disease
diagnosis
due
their
low
fluorescence
background
significant
enhancement
towards
the
target.
This
review
provides
research
progress
that
target
polarity,
pH
biological
species
(reactive
oxygen
species,
biothiols,
biomacromolecules,
etc.)
over
last
five
years.
In
particular,
we
emphasise
molecular
design
strategies,
mechanisms,
application
of
these
probes.
As
such,
this
aims
provide
guidance
enable
researchers
develop
new
improved
probes,
as
well
promoting
use
sensing,
imaging,
therapy.
Angewandte Chemie International Edition,
Journal Year:
2021,
Volume and Issue:
60(28), P. 15418 - 15425
Published: May 4, 2021
Abstract
Selective
fluorescence
imaging
of
biomarkers
in
vivo
and
situ
for
evaluating
orthotopic
hepatocellular
carcinoma
(HCC)
chemotherapy
remains
a
great
challenge
due
to
current
agents
suffering
from
the
potential
interferences
other
hydrolases.
Herein,
we
observed
that
carbamate
unit
showed
high
selectivity
toward
HCC‐related
biomarker
carboxylesterase
(CE)
evaluation
treatment.
A
near‐infrared
two‐photon
fluorescent
probe
was
developed
not
only
specially
image
CE
activity
but
also
target
liver
tumor
after
systemic
administration.
The
signals
correlate
well
with
apoptosis,
making
it
possible
evaluate
status
enables
high‐resolution
three‐dimensional
view
first
time.
This
study
may
promote
advances
optical
approaches
precise
imaging‐guided
diagnosis
HCC
its
Aggregate,
Journal Year:
2021,
Volume and Issue:
2(4)
Published: May 7, 2021
Abstract
Activity‐based
approaches
for
designing
AIEgens
possess
prominent
advantages
including
high
selectivity,
sensitivity,
and
signal‐to‐noise
ratio,
they
have
received
more
attention
in
recent
years.
Excellent
activatable
AIE
probes
been
reported
detecting
toxic
substances,
imaging
intracellular
active
molecules/biomolecules,
as
well
monitoring
the
activity
of
overexpressed
enzymes
cancers.
Moreover,
majority
theranostic
can
be
specifically
triggered
cancer
cells
kill
these
under
light
irradiation,
while
no
distinct
effect
on
normal
cells,
demonstrating
satisfactory
therapeutic
selectivity
that
is
superior
to
traditional
chemotherapy.
Thus,
this
review,
we
systematically
summarized
development
bioprobes
years
from
molecular
design
principles
biological
applications.
The
challenges
corresponding
solutions
are
described.
We
hope
information
provided
review
will
facilitate
promote
practical
application
AIEgens.
