CRISPR-based diagnostics

Nature Biomedical Engineering, Journal Year: 2021, Volume and Issue: 5(7), P. 643 - 656

Published: July 16, 2021

Language: Английский

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Device integration of electrochemical biosensors

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(5), P. 346 - 360

Published: Feb. 24, 2023

Language: Английский

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CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor

Nano Energy, Journal Year: 2020, Volume and Issue: 82, P. 105711 - 105711

Published: Dec. 18, 2020

Language: Английский

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Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

Biosensors and Bioelectronics, Journal Year: 2020, Volume and Issue: 166, P. 112445 - 112445

Published: July 26, 2020

Language: Английский

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Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Sept. 30, 2020

Abstract The CRISPR-Cas12a RNA-guided complexes have tremendous potential for nucleic acid detection but are limited to the picomolar limit without an amplification step. Here, we develop a platform with engineered crRNAs and optimized conditions that enabled us detect various clinically relevant targets higher sensitivity, achieving of in femtomolar range any target pre-amplification By extending 3′- or 5′-ends crRNA different lengths ssDNA, ssRNA, phosphorothioate discover self-catalytic behavior augmented rate LbCas12a-mediated collateral cleavage activity as high 3.5-fold compared wild-type significant improvement specificity recognition. Particularly, 7-mer DNA extension is determined be universal spacer-independent enhancing sensitivity detection. We perform detailed characterization our ENHANCE system modifications, types, reporters, divalent cations. With isothermal SARS-CoV-2 RNA using RT-LAMP, modified incorporated paper-based lateral flow assay can up 23-fold within 40–60 min.

Language: Английский

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CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection

Advanced Science, Journal Year: 2020, Volume and Issue: 7(13)

Published: May 27, 2020

MicroRNAs (miRNAs) have been widely investigated as potential biomarkers for early clinical diagnosis of cancer. Developing an miRNA detection platform with high specificity, sensitivity, and exploitability is always necessary. Electrochemiluminescence (ECL) electrogenerated chemiluminescence technology that greatly decreases background noise improves sensitivity. The development a paper-based ECL biosensor further makes suitable point-of-care detection. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a high-fidelity, efficient, programmable CRISPR RNA (crRNA) guided RNase has brought next-generation biosensing technology. However, existing CRISPR/Cas13a based often faces trade-off between sensitivity specificity. In this research, powered portable chip (PECL-CRISPR) constructed. Wherein target activates Cas13a to cleave well-designed preprimer, triggers the subsequent exponential amplification Under optimized conditions, limit-of-detection 1 × 10

Language: Английский

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CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

Chemical Science, Journal Year: 2021, Volume and Issue: 12(13), P. 4683 - 4698

Published: Jan. 1, 2021

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) protein systems revolutionize genome engineering advance analytical chemistry diagnostic technology.

Language: Английский

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Integrating CRISPR-Cas12a with a DNA circuit as a generic sensing platform for amplified detection of microRNA

Chemical Science, Journal Year: 2020, Volume and Issue: 11(28), P. 7362 - 7368

Published: Jan. 1, 2020

A generic sensing strategy that integrates CRISPR-Cas12a with a DNA circuit is proposed for amplified detection of microRNA.

Language: Английский

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An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for Listeria monocytogenes detection

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 179, P. 113073 - 113073

Published: Feb. 6, 2021

Language: Английский

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CRISPR/cas systems redefine nucleic acid detection: Principles and methods

Biosensors and Bioelectronics, Journal Year: 2020, Volume and Issue: 165, P. 112430 - 112430

Published: July 8, 2020

Language: Английский

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