Applications of mass spectrometry imaging in botanical research DOI Creative Commons
Yijun Chen,

Hai-Sheng Zeng,

Hong‐Lei Jin

et al.

Advanced Biotechnology, Journal Year: 2024, Volume and Issue: 2(1)

Published: Feb. 15, 2024

Mass spectrometry imaging (MSI) serves as a valuable tool enabling researchers to scrutinize various compounds, peptides, and proteins within sample, providing detailed insights at both elemental molecular levels. This innovative technology transforms information obtained from mass spectrometer- encompassing ionic strength, mass-to-charge ratio, ionized molecule coordinates-within defined region into pixel-based model. Consequently, it reconstructs the spatial distribution of ions, allowing for comprehensive understanding landscapes. The significance MSI lies in its ability offer multiple advantages, including straightforward sample preparation remarkable sensitivity, all achieved without necessity labeling. Particularly realm plant biology, finds frequent application examining target metabolites other components tissues. review delves fundamental principles, distinguishing features, merits, applications three prominent technologies. Furthermore, we aim assist readers navigating utilization their biology research by discussing primary challenges, proposing potential solutions, elucidating future prospects associated with this cutting-edge technology.

Language: Английский

Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI DOI Creative Commons
Oliver J. Hale, Helen J. Cooper

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(10), P. 4619 - 4627

Published: March 4, 2021

Previously, we have demonstrated native mass spectrometry imaging (native MSI) in which the spatial distribution of proteins maintained their native-like, folded conformations was determined using liquid extraction surface analysis (LESA). While providing an excellent testbed for proof principle, resolution LESA is currently limited primarily by physical size sampling pipette tip. Here, report adoption nanospray-desorption electrospray ionization (nano-DESI) MSI, delivering substantial improvements versus MSI. In addition, nano-DESI may be used location-targeted top–down proteomics directly from tissue. Proteins, including a homodimeric complex not previously detected were identified through combination collisional activation, high-resolution MS and proton transfer charge reduction.

Language: Английский

Citations

113
Advances in mass spectrometry imaging for spatial cancer metabolomics DOI
Xin Ma, Facundo M. Fernández

Mass Spectrometry Reviews, Journal Year: 2022, Volume and Issue: 43(2), P. 235 - 268

Published: Sept. 6, 2022

Abstract Mass spectrometry (MS) has become a central technique in cancer research. The ability to analyze various types of biomolecules complex biological matrices makes it well suited for understanding biochemical alterations associated with disease progression. Different samples, including serum, urine, saliva, and tissues have been successfully analyzed using mass spectrometry. In particular, spatial metabolomics MS imaging (MSI) allows the direct visualization metabolite distributions tissues, thus enabling in‐depth cancer‐associated changes within specific structures. recent years, MSI studies increasingly used uncover metabolic reprogramming development, discovery key biomarkers potential diagnostics. this review, we aim cover basic principles experiments nonspecialists, fundamentals, sample preparation process, evolution techniques used, data analysis strategies. We also review advances research last 5 lipidomics glycomics, adoption three‐dimensional multimodal approaches, implementation artificial intelligence/machine learning MSI‐based studies. clinical single‐cell is discussed. Spatially resolved on other small molecule metabolites such as amino acids, polyamines, nucleotides/nucleosides will not be discussed context.

Language: Английский

Citations

87
Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues DOI Creative Commons
Xiaoping Zhu, Tianyi Xu, Peng Chen

et al.

Frontiers in Chemistry, Journal Year: 2022, Volume and Issue: 9

Published: Feb. 3, 2022

Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, map hundreds of biomolecules, such as peptides, proteins, lipid, other organic compounds in cells tissues. So far, although several soft ionization desorption electrospray (DESI) secondary ion (SIMS) have been used for matrix-assisted laser desorption/ionization (MALDI) still the most widespread MSI scanning method. Here, we aim to provide comprehensive review MALDI-MSI an emphasis on its advances instrumentation, methods, application, future directions single cell biological

Language: Английский

Citations

83
Recent advances in proteomics and metabolomics in plants DOI Creative Commons
Shijuan Yan, Ruchika Bhawal, Zhibin Yin

et al.

Molecular Horticulture, Journal Year: 2022, Volume and Issue: 2(1)

Published: July 23, 2022

Over the past decade, systems biology and plant-omics have increasingly become main stream in plant research. New developments mass spectrometry bioinformatics tools, methodological schema to integrate multi-omics data leveraged recent advances proteomics metabolomics. These progresses are driving a rapid evolution field of research, greatly facilitating our understanding mechanistic aspects metabolisms interactions plants with their external environment. Here, we review MS-based metabolomics tools workflows special focus on applications research using several case studies related stress response, gene/protein function characterization, metabolic signaling pathways exploration, natural product discovery. We also present projection concerning future perspectives development including challenges for system biology. This is intended provide readers an overview how advanced MS technology, integrated application can be used advance

Language: Английский

Citations

72
Single-cell lipidomics enabled by dual-polarity ionization and ion mobility-mass spectrometry imaging DOI Creative Commons
Hua Zhang, Yuan Liu, Lauren Fields

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 25, 2023

Single-cell (SC) analysis provides unique insight into individual cell dynamics and cell-to-cell heterogeneity. Here, we utilize trapped ion mobility separation coupled with dual-polarity ionization mass spectrometry imaging (MSI) to enable high-throughput in situ profiling of the SC lipidome. Multimodal imaging, which dual-polarity-mode MSI is used perform serial data acquisition runs on cells, significantly enhanced lipidome coverage. High-spatial resolution SC-MSI identifies both inter- intracellular lipid heterogeneity; this heterogeneity further explicated by Uniform Manifold Approximation Projection machine learning-driven classifications. We characterize alteration response stearoyl-CoA desaturase 1 inhibition and, additionally, identify cell-layer specific distribution patterns mouse cerebellar cortex. This integrated multimodal technology enables high-resolution spatial mapping intercellular heterogeneity, remodeling induced pharmacological intervention, region-specific diversity within tissue.

Language: Английский

Citations

47
High spatial resolution mass spectrometry imaging for spatial metabolomics: Advances, challenges, and future perspectives DOI
Siyuan Ma,

Yixin Leng,

Xiaoping Li

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 159, P. 116902 - 116902

Published: Dec. 26, 2022

Language: Английский

Citations

56
Advances in mass spectrometry imaging for plant metabolomics—Expanding the analytical toolbox DOI
Zhibin Yin, Wenjie Huang, Kun Li

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(5), P. 2168 - 2180

Published: July 11, 2024

SUMMARY Mass spectrometry imaging (MSI) has become increasingly popular in plant science due to its ability characterize complex chemical, spatial, and temporal aspects of metabolism. Over the past decade, as emerging unique features various MSI techniques have continued support new discoveries studies metabolism closely associated with function physiology, spatial metabolomics based on positioned it at forefront metabolic studies, providing opportunity for far higher resolution than was previously available. Despite these efforts, profound challenges levels resolution, sensitivity, quantitative ability, chemical confidence, isomer discrimination, multi‐omics integration, undoubtedly remain. In this Perspective, we provide a contemporary overview emergent widely used sciences, particular emphasis recent advances methodological breakthroughs. Having established detailed context MSI, outline both golden opportunities key currently facing metabolomics, presenting our vision how enormous potential technologies will contribute progress coming years.

Language: Английский

Citations

11
Chemical Analysis of Single Cells and Organelles DOI Creative Commons
Keke Hu, Tho D. K. Nguyen, Stefania Rabasco

et al.

Analytical Chemistry, Journal Year: 2020, Volume and Issue: 93(1), P. 41 - 71

Published: Dec. 7, 2020

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTChemical Analysis of Single Cells and OrganellesKeke HuKeke HuDepartment Chemistry Molecular Biology, University Gothenburg, Kemivägen 10, 41296 SwedenMore by Keke HuView Biography, Tho D. K. NguyenTho NguyenDepartment NguyenView Stefania RabascoStefania RabascoDepartment RabascoView Pieter E. OomenPieter OomenDepartment SwedenParaMedir B.V., 1e Energieweg 13, 9301 LK Roden, The NetherlandsMore OomenView Andrew G. Ewing*Andrew EwingDepartment Sweden*Email: [email protected]More EwingView Biographyhttp://orcid.org/0000-0002-2084-0133Cite this: Anal. Chem. 2021, 93, 1, 41–71Publication Date (Web):December 7, 2020Publication History Published online7 December 2020Published inissue 12 January 2021https://doi.org/10.1021/acs.analchem.0c04361Copyright © 2020 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoicewith CC-BYlicenseArticle Views5015Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (14 MB) Get e-AlertsSUBJECTS:Vesicles,Peptides proteins,Labeling,Microscopy,Electrochemical cells e-Alerts

Language: Английский

Citations

54
Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances DOI Creative Commons
Yaolei Li,

Zhijian Lin,

Yu Wang

et al.

Arabian Journal of Chemistry, Journal Year: 2022, Volume and Issue: 15(11), P. 104302 - 104302

Published: Sept. 27, 2022

Traditional Chinese medicine (TCM) is the key to unlock treasures of civilization. TCM and its compound play a beneficial role in medical activities cure diseases, especially major public health events such as novel coronavirus epidemics across globe. The chemical composition formula complex diverse, but their effective substances resemble "mystery boxes". Revealing active ingredients mechanisms action has become focal point difficulty research for herbalists. Although existing methods are numerous constantly updated iteratively, there remain lack prospective reviews. Hence, this paper provides comprehensive account new approaches technologies based on previous studies with an vitro vivo perspective. In addition, bottlenecks also revealed. Especially, we look ahead perspectives, applications future development. This work reviews perspectives open horizons research. Consequently, herbal compounding pharmaceutical study should carry essence while pursuing innovations field.

Language: Английский

Citations

34
Mass Spectrometry Imaging for Single-Cell or Subcellular Lipidomics: A Review of Recent Advancements and Future Development DOI Creative Commons

Dan Li,

Zheng Ouyang, Xiaoxiao Ma

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(6), P. 2712 - 2712

Published: March 17, 2023

Mass Spectrometry Imaging (MSI) has emerged as a powerful imaging technique for the analysis of biological samples, providing valuable insights into spatial distribution and structural characterization lipids. The advancements in high-resolution MSI have made it an indispensable tool single-cell or subcellular lipidomics. By preserving both intracellular intercellular information, enables comprehensive lipidomics individual cells organelles. This researchers to delve deeper diversity lipids within understand role shaping cell behavior. In this review, we aim provide overview recent future prospects cellular/subcellular keeping abreast cutting-edge studies field, will continue push boundaries understanding lipid metabolism impact on cellular

Language: Английский

Citations

20