Angewandte Chemie International Edition,
Journal Year:
2021,
Volume and Issue:
60(28), P. 15426 - 15435
Published: April 1, 2021
Abstract
A
general
strategy
to
carry
out
cell
uptake
and
biodistribution
studies
is
label
nanoparticles
(NPs)
with
a
fluorescent
dye.
However,
the
comparative
study
of
different
dye‐loaded
NPs
remains
difficult
owing
uncontrolled
dye
quenching
de‐quenching.
Here
we
compared
two
types
dye‐labeled
demonstrated
their
distinct
properties.
molecules
at
solid
state
suffer
from
quenching,
so
release
and/or
NP
degradation
in
biological
environments
leads
several‐fold
increase
fluorescence
intensity
despite
same
amount
NPs,
switch
In
contrast,
soluble
exhibit
no
effect.
To
standardize
study,
propose
possible
solutions:
using
lower
loading
or
medium
analysis
for
quantifying
NPs.
This
work
provides
valuable
insights
into
selecting
valid
quantification
methods
bio‐nano
studies.
Chemosensors,
Journal Year:
2025,
Volume and Issue:
13(2), P. 67 - 67
Published: Feb. 13, 2025
Near-infrared
(NIR)
light
is
a
promising
tool
for
biomedical
imaging
and
therapy,
offering
excellent
tissue
penetration,
low
scattering,
minimal
biological
fluorescence
interference.
An
NIR-II
optical
range
of
900–1880
nm
with
reduced
background
interference
particularly
useful
disease
diagnosis
treatment.
Probes
based
on
organic
molecules
are
gaining
attention
their
structural
flexibility
stable
performance.
Organic
molecular
aggregates,
such
as
J-aggregates,
H-aggregates,
aggregation-induced
emission
(AIE)-aggregates,
exhibit
unique
properties
like
tunable
spectral
shifts,
improved
photostability,
higher
absorption
quantum
yields.
This
mini
review
briefly
discusses
the
advancements
in
therapy
technologies,
focusing
classification,
formation
mechanisms,
applications
aggregates
treatment,
theoretical
foundation
practical
guidance
future
research.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 28, 2025
Abstract
Three‐photon
phosphorescence
microscopic
bioimaging
holds
promise
for
deep‐tissue
time‐resolved
brain
imaging
with
high
spatial
resolution
and
contrast.
However,
developing
probes
bright
strong
second
near‐infrared
(NIR‐II)
three‐photon
absorption
suitable
biological
applications
remains
a
formidable
challenge.
Herein,
kind
of
fluorescence
resonance
energy
transfer
(FRET)‐based
nanoparticles
(NPFA‐PorPt
NPs)
is
proposed
by
co‐encapsulation
absorbing
aggregation‐induced
emission
luminogen
(NPFA),
phosphorescent
platinum
octaethylporphyrin
(PorPt)
using
1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐[methoxy(polyethylene
glycol)‐2000]
as
the
encapsulation
matrix.
NPFA
well
designed
to
display
superior
properties
in
NIR‐II
region
its
overlaps
PorPt,
allowing
efficient
PorPt
nanoparticles.
The
optimal
NPFA‐2%PorPt
NPs
boosted
350‐fold
compared
that
pure
aggregates
upon
same
excitation.
excited
enables
be
successfully
applied
vivo
muscle
vascular
deep
penetration,
resolution,
contrast,
even
small
capillaries
tissue
can
recognized.
This
study
paves
way
development
highly
multiphoton‐absorbing
biomedical
applications.
View,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 21, 2025
Abstract
Environmentally
responsive
fluorophores,
such
as
those
with
aggregation‐caused
quenching
(ACQ)
and
Förster
resonance
energy
transfer
(FRET)
properties,
are
widely
used
to
track
nanocarriers
while
minimizing
artifacts.
This
study
compares
their
applicability
accuracy
when
an
ACQ
probe
(FD‐B21)
a
FRET
pair
(DiD/DiR)
label
polymeric
micelles
(PMs)
model
nanocarriers.
In
vitro,
the
PM
showed
unexpected
increases
in
fluorescence
biological
samples,
indicating
incomplete
aggregation
after
release.
For
pair,
efficiency
is
inadequate
for
assessing
nanocarrier
integrity
at
low
donor
acceptor
intensities.
cell‐based
experiments,
probes
demonstrated
minimal
reillumination
(1.8%)
4
h
of
incubation
Raw
264.7
cells,
whereas
produced
up
72%
false‐positive
signals.
vivo,
mouse
liver
increased
modestly
from
5%
18%
over
24
h,
which
was
significantly
lower
than
75%
increase
observed
pair.
Additionally,
vivo
crosstalk
contributed
approximately
20%
These
findings
emphasize
that
probes,
although
sensitive
surfactant‐rich
vitro
environments,
prone
signals,
more
reliable
cell
imaging
because
properties.
Angewandte Chemie International Edition,
Journal Year:
2021,
Volume and Issue:
60(28), P. 15426 - 15435
Published: April 1, 2021
Abstract
A
general
strategy
to
carry
out
cell
uptake
and
biodistribution
studies
is
label
nanoparticles
(NPs)
with
a
fluorescent
dye.
However,
the
comparative
study
of
different
dye‐loaded
NPs
remains
difficult
owing
uncontrolled
dye
quenching
de‐quenching.
Here
we
compared
two
types
dye‐labeled
demonstrated
their
distinct
properties.
molecules
at
solid
state
suffer
from
quenching,
so
release
and/or
NP
degradation
in
biological
environments
leads
several‐fold
increase
fluorescence
intensity
despite
same
amount
NPs,
switch
In
contrast,
soluble
exhibit
no
effect.
To
standardize
study,
propose
possible
solutions:
using
lower
loading
or
medium
analysis
for
quantifying
NPs.
This
work
provides
valuable
insights
into
selecting
valid
quantification
methods
bio‐nano
studies.
