Early life cow's milk allergy prevention DOI Open Access
Mengshan Liu

Published: Sept. 5, 2023

The prevalence and lack of good solutions for cow's milk allergy (CMA) in young children are leading to public concern increasing socioeconomic problems. This requires new preventive approaches. In this thesis, we investigated the potential an oral formulation – based on poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles (NP) selected peptides from whey proteins CpG-Oligodeoxynucleotides CMA prevention, using both vitro vivo studies. vitro, showed, through Förster Resonance Energy Transfer (FRET) imaging living cells, that PLGA NP protected encapsulated up 6 hours simulated gastric fluid extended their residence time isolated mouse dendritic cells 96 hours. demonstrates hypothesis improved stability peptide cargo gastrointestinal lumen retention intestinal DC upon internalization. vivo, showed pretreatment with two PLGA-NP-encapsulated peptides, prior allergic sensitization whey, mice acute skin reactions intradermal exposure whey. addition, have shown protection is dose-related may be associated whey-specific systemic silencing ex whey-stimulated TNF-α release by splenocytes. Intriguingly, superior tolerogenic effect orally administered co-loaded T-cell epitopes containing a CpG adjuvant as compared separately counterpart. combination prevented rise specific IgE antibody levels serum whey-induced reactions, beneficial immunomodulatory immune cells. Finally, allergy-associated effects empty, or CpG-containing were evaluated derived human monocytes. immature moDCs primed CpG-ODN loaded also effectively increased ratios Treg/Th2 Th1/Th2. finding supports co-encapsulated facilitates inhibition Th2 immunity vivo. conclusion, our findings substantiate translational which future development effective safe strategies early life prevention (i.e., supplementing hydrolyzed formula infants at risk). besides BLG-peptides motives, include bacterial DNA strains, can increase efficacy formulations tolerance induction. strategy further developed modality treat cow’s example adjunct treatment allergen immunotherapy.

Language: Английский

Nanoparticle cellular internalization is not required for RNA delivery to mature plant leaves DOI
Huan Zhang, Natalie S. Goh, Jeffrey W. Wang

et al.

Nature Nanotechnology, Journal Year: 2021, Volume and Issue: 17(2), P. 197 - 205

Published: Nov. 22, 2021

Language: Английский

Citations

150
Design of Stimuli‐Responsive Peptides and Proteins DOI Creative Commons
Yang Li, Guangze Yang, Lukas Gerstweiler

et al.

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 33(7)

Published: Dec. 7, 2022

Abstract Stimuli‐responsive peptides and proteins are an exciting class of smart biomaterials for various applications have received significant attention over the past decades. A wide variety stimuli such as temperature, pH, ions, enzymes, magnetic field, redox, etc., explored. This article provides a review five intensively studied types stimuli‐responsive proteins, their design principles applications, including temperature‐, pH‐, light‐, metal ion‐, enzyme‐responsive with emphasis on key concepts switch function. Moreover, typical examples discussed to provide better understanding concept underlying methodology. will facilitate inspire future innovation toward new peptide‐ protein‐based materials diverse applications.

Language: Английский

Citations

37
Concurrent Detection of Protein and miRNA at the Single Extracellular Vesicle Level Using a Digital Dual CRISPR-Cas Assay DOI
Xun Xu, Yuanyue Zhang, Jiajia Liu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

The simultaneous detection of proteins and microRNA (miRNA) at the single extracellular vesicle (EV) level shows great promise for precise disease profiling, owing to heterogeneity scarcity tumor-derived EVs. However, a highly reliable method multiple-target analysis EVs remains be developed. In this study, digital dual CRISPR-Cas-powered Single EV Evaluation (ddSEE) system was proposed enable concurrent surface protein inner miRNA single-molecule level. By optimizing reaction conditions CRISPR-Cas12a CRISPR-Cas13a, detected by Cas12a using antibody-DNA conjugates transfer signal DNA, while analyzed Cas13a through EV-liposome fusion. A microfluidic chip containing 188,000 microwells used convert CRISPR-Cas into assay format absolute quantification miRNA/protein-positive without bias fluorescence imaging, which can detect as few 214 EVs/μL. Finally, total 31 blood samples, 21 from breast cancer patients 10 healthy donors, were collected tested, achieving diagnostic accuracy 92% in distinguishing with donors. With its quantification, ease use, multiplexed capability, ddSEE demonstrates potential both research clinical applications.

Language: Английский

Citations

7
FRET Ratiometric Nanoprobes for Nanoparticle Monitoring DOI Creative Commons
Guangze Yang, Yun Liu,

Jisi Teng

et al.

Biosensors, Journal Year: 2021, Volume and Issue: 11(12), P. 505 - 505

Published: Dec. 9, 2021

Fluorescence labelling is often used for tracking nanoparticles, providing a convenient assay monitoring nanoparticle drug delivery. However, it difficult to be quantitative, as many factors affect the fluorescence intensity. Förster resonance energy transfer (FRET), taking advantage of from donor fluorophore an acceptor fluorophore, provides distance ruler probe NP This article review different FRET approaches ratiometric self-assembly and formation their in vivo fate, integrity release. We anticipate that fundamental understanding gained these studies will offer new insights into design nanoparticles with improved better-controlled properties.

Language: Английский

Citations

35
Computationally guided design and synthesis of dual‐drug loaded polymeric nanoparticles for combination therapy DOI Creative Commons
Song Jin, Zhenwei Lan, Guangze Yang

et al.

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: June 3, 2024

Abstract Single‐drug therapies or monotherapies are often inadequate, particularly in the case of life‐threatening diseases like cancer. Consequently, combination emerge as an attractive strategy. Cancer nanomedicines have many benefits addressing challenges faced by small molecule therapeutic drugs, such low water solubility and bioavailability, high toxicity, etc. However, it remains a significant challenge encapsulating two drugs nanoparticle. To address this issue, computational methodologies employed to guide rational design synthesis dual‐drug‐loaded polymer nanoparticles while achieving precise control over drug loading. Based on sequential nanoprecipitation technology, five factors identified that affect formulation candidates into dual‐drug loaded nanoparticles, then screened 176 formulations under different experimental conditions. these data, machine learning methods applied pin down key factors. The implementation methodology holds potential significantly mitigate complexities associated with co‐assembly compounds nanoparticulate systems demonstrates promising avenue for therapy. This approach provides new strategy enabling streamlined, high‐throughput screening nanoscale drug‐loaded entities.

Language: Английский

Citations

6
Rational design of ROS scavenging and fluorescent gold nanoparticles to deliver siRNA to improve plant resistance to Pseudomonas syringae DOI Creative Commons
Jie Qi, Yanhui Li, Xue Yao

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 29, 2024

Bacterial diseases are one of the most common issues that result in crop loss worldwide, and increasing usage chemical pesticides has caused occurrence resistance pathogenic bacteria environmental pollution problems. Nanomaterial mediated gene silencing is starting to display powerful efficiency friendliness for improving plant disease resistance. However, internalization nanomaterials physiological mechanisms behind nano-improved still rarely understood. We engineered polyethyleneimine (PEI) functionalized gold nanoparticles (PEI-AuNPs) with fluorescent properties ROS scavenging activity act as siRNA delivery platforms. Besides loading, protection, nucleic acid molecules mature leaf cells by PEI-AuNPs, its property further enables traceability distribution loaded cells. Additionally, PEI-AuNPs-based RNAi system successfully defense-regulated AtWRKY1. Compared control plants, silenced plants performed better Pseudomonas syringae, showing a reduced bacterial number, decreased content, increased antioxidant enzyme activities, improved chlorophyll fluorescence performance. Our results showed advantages AuNP-based technology resistance, well potential nanobiotechnology protect agricultural production. Polyethyleneimine reactive oxygen species (ROS) ability synthesized rationally; PEI-AuNPs indicator evaluate nuclear efficiency; platform mediate AtWRKY1 Pst DC3000 ROS, activity, photosynthesis.

Language: Английский

Citations

5
Influence of nanoparticle mechanical property on protein corona formation DOI

Tengjisi,

Yue Hui,

Yuanyuan Fan

et al.

Journal of Colloid and Interface Science, Journal Year: 2021, Volume and Issue: 606, P. 1737 - 1744

Published: Aug. 24, 2021

Language: Английский

Citations

31
Quantitative Analysis of Macrophage Uptake and Retention of Fluorescent Organosilica Nanoparticles: Implications for Nanoparticle Delivery and Therapeutics DOI Creative Commons
Hung-Chang Chou,

Shih-Jiuan Chiu,

Teh‐Min Hu

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(4), P. 3656 - 3667

Published: Feb. 2, 2024

This study investigates the uptake and retention of stable fluorescent organosilica nanoparticles by macrophages, which play a vital role in scavenging environmental nanomedicines within body. We used rhodamine 6G-loaded (SiNP-R6G) synthesized from thiol-functionalized organosilane precursor. Our primary objective was to establish quantitative relationship between measurements nanoparticle tracking analysis, enabling precise "counting" taken up macrophages under kinetic measurement conditions. demonstrated concentration-dependent, saturable internalization model macrophage (RAW 264.7 cells), with maximum rate (Vmax) 7.9 × 104 per hour cell. The estimated number concentration for half-maximum approximately 0.8 trillion milliliter, significant portion (∼80%) internalized SiNP-R6G remained entrapped cells 48 h, indicating sustained particle capacity macrophages. data further demonstrate that occupy more than 10% total cell volume, without inducing cytotoxic effects. heightened is attributed intracellular vesicles capable accommodating 50 densely packed, yet nonaggregated, single vesicle. In summary, our underscores effective development methodology cellular providing valuable insights into kinetics capabilities nanoparticle-based drug delivery.

Language: Английский

Citations

4
Synergistic enhancement of low-dose radiation therapy via cuproptosis and metabolic reprogramming for radiosensitization in in situ hepatocellular carcinoma DOI Creative Commons

Ni Shao,

Yongqing Yang,

Genwen Hu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 19, 2024

Radiotherapy (RT) is a primary clinical approach for cancer treatment, but its efficacy often hindered by various challenges, especially radiation resistance, which greatly compromises the therapeutic effectiveness of RT. Mitochondria, central to cellular energy metabolism and regulation cell death, play critical role in mechanisms radioresistance. In this context, cuproptosis, novel copper-induced mitochondria-respiratory-dependent death pathway, offers promising avenue radiosensitization. study, an innovative theranostic nanoplatform was designed induce cuproptosis synergy with low-dose therapy (LDRT, i.e., 0.5–2 Gy) treatment situ hepatocellular carcinoma (HCC). This aims reverse hypoxic tumor microenvironment, promoting shift from glycolysis oxidative phosphorylation (OXPHOS), thereby enhancing sensitivity cuproptosis. Concurrently, Fenton-like reaction ensures sustained supply copper depletion glutathione (GSH), inducing disrupting mitochondrial function, interrupting supply. strategy effectively overcomes radioresistance enhances against tumors. conclusion, study elucidates intricate interactions among hypoxia reversal, metabolic reprogramming, radiosensitization, particularly context treating carcinoma, providing paradigm radiotherapy.

Language: Английский

Citations

4
Liquid core fluorescent organic nanoprobes: Long-term stability and highly selective lipid droplets bio-imaging DOI
Lihua Tang,

Didi Hu,

Jingyu Feng

et al.

Talanta, Journal Year: 2023, Volume and Issue: 267, P. 125169 - 125169

Published: Sept. 8, 2023

Language: Английский

Citations

10