Science Advances,
Journal Year:
2022,
Volume and Issue:
8(26)
Published: June 29, 2022
DNA
aptamers
have
been
widely
used
as
biosensors
for
detecting
a
variety
of
targets.
Despite
decades
success,
they
not
applied
to
monitor
any
targets
in
plants,
even
though
plants
are
major
platform
providing
oxygen,
food,
and
sustainable
products
ranging
from
energy
fuels
chemicals,
high-value
such
pharmaceuticals.
A
barrier
progress
is
lack
efficient
methods
deliver
into
plant
cells.
We
herein
report
thiol-mediated
uptake
method
that
more
efficiently
delivers
Arabidopsis
tobacco
leaf
cells
than
another
state-of-the-art
method,
nanostructures.
Such
allowed
delivery
glucose
aptamer
sensor
sensing
glucose.
This
demonstration
opens
new
avenue
apply
sensors
functional
studies
various
targets,
including
metabolites,
hormones,
metal
ions,
proteins
better
understanding
the
biodistribution
regulation
these
species
their
functions.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(7), P. 3142 - 3149
Published: Feb. 10, 2022
With
the
growing
consumption
of
caffeine-containing
beverages,
detection
caffeine
has
become
an
important
biomedical,
bioanalytical,
and
environmental
topic.
We
herein
isolated
four
high-quality
aptamers
for
with
dissociation
constants
ranging
from
2.2
to
14.6
μM
as
characterized
using
isothermal
titration
calorimetry.
Different
binding
patterns
were
obtained
three
single
demethylated
analogues:
theobromine,
theophylline,
paraxanthine,
highlighting
effect
molecular
symmetry
arrangement
methyl
groups
in
caffeine.
A
structure-switching
fluorescent
sensor
was
designed
showing
a
limit
1.2
caffeine,
which
reflected
labeled
concentration
within
6.1%
difference
eight
commercial
beverages.
In
20%
human
serum,
4.0
achieved.
aptamer
sensors
forming
array,
analogues
well
separated
nine
other
closely
related
molecules.
ACS Applied Materials & Interfaces,
Journal Year:
2022,
Volume and Issue:
15(1), P. 138 - 157
Published: April 8, 2022
Neurotransmitters,
as
important
chemical
small
molecules,
perform
the
function
of
neural
signal
transmission
from
cell
to
cell.
Excess
concentrations
neurotransmitters
are
often
closely
associated
with
brain
diseases,
such
Alzheimer's
disease,
depression,
schizophrenia,
and
Parkinson's
disease.
On
other
hand,
release
under
induced
stimulation
indicates
occurrence
reward-related
behaviors,
including
food
drug
addiction.
Therefore,
understand
physiological
pathological
functions
neurotransmitters,
especially
in
complex
environments
living
brain,
it
is
urgent
develop
effective
tools
monitor
their
dynamics
high
sensitivity
specificity.
Over
past
30
years,
significant
advances
electrochemical
sensors
optical
probes
have
brought
new
possibilities
for
studying
neurons
circuits
by
monitoring
changes
neurotransmitters.
This
Review
focuses
on
progress
construction
vivo
analysis
summarizes
current
attempts
address
key
issues
development
selectivity,
sensitivity,
stability.
Combined
latest
technologies
methods,
several
strategies
sensor
provided
recording
environment
brain.
Environmental Science & Technology,
Journal Year:
2022,
Volume and Issue:
56(24), P. 17702 - 17711
Published: Nov. 28, 2022
Estrogenic
compounds
such
as
estrone
(E1),
17β-estradiol
(E2),
and
17α-ethynylestradiol
(EE2)
are
serious
environmental
contaminants
due
to
their
potent
biological
activities.
At
least
six
selections
were
previously
reported
obtain
DNA
aptamers
for
E2,
highlighting
its
importance.
A
careful
analysis
revealed
that
the
previous
either
too
long
or
do
not
bind
optimally.
Herein,
a
series
of
new
obtained
from
capture-SELEX
method
with
dissociation
constants
down
30
nM
determined
by
isothermal
titration
calorimetry
(ITC).
Two
converted
structure-switching
fluorescent
biosensors,
which
achieved
limit
detection
3.3
9.1
respectively.
One
aptamer
showed
similar
binding
affinities
all
three
estrogens,
while
other
is
more
selective
E2.
Both
required
Mg2+
binding.
The
proposed
sensors
successfully
applied
in
determination
E2
wastewater.
Moreover,
comparisons
made
based
on
primary
sequence
alignment
secondary
structures.
Among
truncated
aptamers,
ITC
only
one
them.
newly
selected
have
combined
advantages
small
size
high
affinities.
Science Advances,
Journal Year:
2022,
Volume and Issue:
8(26)
Published: June 29, 2022
DNA
aptamers
have
been
widely
used
as
biosensors
for
detecting
a
variety
of
targets.
Despite
decades
success,
they
not
applied
to
monitor
any
targets
in
plants,
even
though
plants
are
major
platform
providing
oxygen,
food,
and
sustainable
products
ranging
from
energy
fuels
chemicals,
high-value
such
pharmaceuticals.
A
barrier
progress
is
lack
efficient
methods
deliver
into
plant
cells.
We
herein
report
thiol-mediated
uptake
method
that
more
efficiently
delivers
Arabidopsis
tobacco
leaf
cells
than
another
state-of-the-art
method,
nanostructures.
Such
allowed
delivery
glucose
aptamer
sensor
sensing
glucose.
This
demonstration
opens
new
avenue
apply
sensors
functional
studies
various
targets,
including
metabolites,
hormones,
metal
ions,
proteins
better
understanding
the
biodistribution
regulation
these
species
their
functions.
