Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies DOI Creative Commons
Wanchong He,

Siyu Dong,

Qinghua Zeng

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1025 - 1025

Published: Feb. 24, 2025

Mitochondria, as vital organelles, play a central role in subcellular research and biomedical innovation. Although functional nucleic acid (FNA) nanostructures have witnessed remarkable progress across numerous biological applications, strategies specifically tailored to target mitochondria for molecular imaging therapeutic interventions remain scarce. This review delves into the latest advancements leveraging FNA mitochondria-specific cancer therapy. Initially, we explore creation of FNA-based biosensors localized mitochondria, enabling real-time detection visualization critical molecules essential mitochondrial function. Subsequently, examine developments aimed at mitochondrial-targeted treatments, including modular nanodevices precise delivery agents programmable disrupting processes. Emphasis is placed on elucidating chemical principles underlying design mitochondrial-specific nanotechnology diverse uses. Lastly, address unresolved challenges outline prospective directions, with goal advancing field encouraging sophisticated tools both academic inquiry clinical applications centered mitochondria.

Language: Английский

Dual-Signal Amplification Strategy Based on Catalytic Hairpin Assembly and APE1-Assisted Amplification for High-Contrast miRNA Imaging in Living Cells DOI
Ya Zhang, Mengxu Sun, Juan Xie

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(2), P. 910 - 916

Published: Jan. 3, 2024

Early tumor diagnosis is crucial to successful treatment. Earlier studies have shown that microRNA a biomarker for early diagnosis. The development of highly sensitive miRNA detection methods, especially in living cells, plays an indispensable role and treatment tumor. Although the catalytic hairpin assembly (CHA)-based analysis strategy commonly used disease diagnosis, further application CHA hindered due its low amplification efficiency recognition contrast. To address these limitations, we propose dual-signal based on APE1-assisted amplification, enabling high-contrast imaging. miR-221 was selected as target model. This has exhibited high efficiency, which could analyze 21 fM. also specificity, distinguish nontarget with single-base differences. Moreover, this method showed significant potential practical application, it successfully expression difference plasma samples normal people patients. Most importantly, level APE1 enzyme cells higher than allowing sensitively specifically image within cells. proposed been indicate fluctuations intracellular between cancer We anticipate will provide fresh insights can be powerful tool analysis.

Language: Английский

Citations

16
A digital platform for One-Pot signal enhanced foodborne pathogen detection based on mesophilic argonaute-driven polydisperse microdroplet reactors and machine learning DOI
Junping Wen,

Minjie Han,

Feng Niu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 482, P. 148845 - 148845

Published: Jan. 21, 2024

Language: Английский

Citations

16
A universal nucleic acid detection platform combing CRISPR/Cas12a and strand displacement amplification with multiple signal readout DOI
Li Tian, Jinjin Wang,

Jiaoyuan Fang

et al.

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125922 - 125922

Published: March 18, 2024

Language: Английский

Citations

13
A novel photoelectrochemical biosensor for sensitive detection of nucleic acids based on recombinase polymerase amplification and 3D-array titania nanorods DOI

Xuan Zhao,

Xiangde Lai,

Bingdong Yan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 296, P. 139528 - 139528

Published: Jan. 7, 2025

Language: Английский

Citations

1
Stimulus-Responsive DNA Hydrogel Biosensors for Food Safety Detection DOI Creative Commons
Huiyuan Wang, Xinyu Wang, Keqiang Lai

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 320 - 320

Published: Feb. 24, 2023

Food safety has always been a major global challenge to human health and the effective detection of harmful substances in food can reduce risk health. However, industry plagued by lack sensitive monitoring methods due tension between cost effectiveness monitoring. DNA-based hydrogels combine advantages biocompatibility, programmability, molecular recognition DNA molecules, hydrophilicity hydrogels, making them hotspot research field new nanomaterials. The stimulus response property greatly broadens function application range hydrogel. In recent years, based on stimulus-responsive mechanisms have widely applied biosensing for variety target substances, including various contaminants. this review, we describe advances preparation stimuli-responsive highlighting progress its detection. Finally, also discuss challenges future hydrogels.

Language: Английский

Citations

22
Recent progress on nanozymes in electrochemical sensing DOI
Wenting Wu, Jing Li

Journal of Electroanalytical Chemistry, Journal Year: 2023, Volume and Issue: 936, P. 117391 - 117391

Published: March 27, 2023

Language: Английский

Citations

16
Research on a Magnetic Separation-Based Rapid Nucleic Acid Extraction System and Its Detection Applications DOI Creative Commons
Li Yao, Sha Liu, Yuanyuan Wang

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(10), P. 903 - 903

Published: Sept. 23, 2023

Nucleic acid extraction represents the “first step” in molecular diagnostic experiments. The quality of this serves as a fundamental prerequisite for ensuring accuracy nucleic detection. This article presents comprehensive design scheme rapid automated system based on magnetic separation. and implementation are analyzed investigated in-depth, focusing core methods, hardware control, software control system. Additionally, study evaluation were carried out concerning detection aspects encompassed by results demonstrate that temperature deviation lysis elution fluids is approximately ±1 °C, positioning system’s movement ±0.005 mm, average bead recovery rate 94.98%, 91.83%. developed manual methods employed sample extraction, enabling isolation highly pure acids from bacteria, blood, animal tissues RT-PCR instrument employs temperatures ranging 70–80 80 drying time 5–10 min, with total less than 35 min different types. Overall, yields high concentration purity, exhibits stable operation, good repeatability, efficiency, low cost. It meets requirements genetic-level research worthy clinical promotion usage.

Language: Английский

Citations

15
A microfluidic card-based electrochemical assay for the detection of sulfonamide resistance genes DOI Creative Commons
Patrick Severin Sfragano, Eduardo C. Reynoso, Norma Elena Rojas-Ruíz

et al.

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125718 - 125718

Published: Jan. 24, 2024

Most electroanalytical detection schemes for DNA markers require considerable time and effort from expert personnel to thoroughly follow the analysis obtain reliable outcomes. This work aims present an electrochemical assay performed inside a small card-based platform powered by microfluidic manipulation, requiring minimal human intervention consumables. The couples sample/signal dual amplification DNA-modified magnetic particles of products. Particularly, sul1 sul4 genes involved in resistance against sulfonamide antibiotics were analyzed. As recognized World Health Organization, antimicrobial threatens global public health hampering medication efficacy infections. Consequently, analytical methods determination such environmental clinical matrices are imperative. Herein, extracted E. coli cells amplified using enzyme-assisted isothermal at 37 °C. products analyzed easily-produced, low-cost, set-up implementing system, demanding limited manual sample volumes. target amplicon was thus captured isolated versatile beads injected into microchannel exposed various reagents continuously controlled flow. After optimization efficiency each phase assay, achieved limits detections 44.2 pmol L−1 48.5 sul4, able detect down ≥500-fold diluted living around 1 h, enabling numerous end-point analyses with single reaction.

Language: Английский

Citations

6
Advances, challenges, and opportunities for food safety analysis in the isothermal nucleic acid amplification/CRISPR-Cas12a era DOI

Pengpeng Xue,

Yubo Peng, Renjing Wang

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16

Published: April 24, 2024

Global food safety stands out as a prominent public concern, affecting populations worldwide. The recurrent challenge of incidents reveals the need for robust inspection framework. In recent years, integration isothermal nucleic acid amplification with CRISPR-Cas12a techniques has emerged promising tool molecular detection hazards, presenting next generation biosensing detection. This paper provides comprehensive review current state research on synergistic application and technology in field safety. innovative combination not only enriches analytical tools, but also improving assay performance such sensitivity specificity, addressing limitations traditional methods. summarized various methodologies by diverse concerns, including pathogenic bacterium, viruses, mycotoxins, adulteration, genetically modified foods. Each section elucidates specific strategies employed highlights advantages conferred. Furthermore, discussed challenges faced this context safety, offering insightful discussions potential solutions future prospects.

Language: Английский

Citations

6
Nanogenosensors based on aptamers and peptides for bioelectrochemical cancer detection: an overview of recent advances in emerging materials and technologies DOI Creative Commons
Babak Mikaeeli Kangarshahi, Seyed Morteza Naghib

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(2)

Published: Jan. 31, 2024

Abstract Electrochemical biosensors fabricated based on nucleic acids have shown great potential for cancer recognition because of their low cost, fast feedback, high sensitivity, and easy operation. This review will demonstrate the impression recent advances applications electrochemical that are acid-based detection. We compare formulated with those formed antibodies highlight some examples developed detection, such as use DNA or RNA aptamers to detect prostate-specific antigens, microRNA-21, carcinoembryonic antigens. discuss rewards drawbacks these challenges they face, stability, reproducibility, interference, standardization. also suggest possible directions opportunities future research development, developing novel acid elements, exploring new transducer materials configurations, designing signal amplification strategies, integrating microfluidic devices portable instruments, evaluating in clinical settings actual samples from patients healthy donors. Overall, we believe offer an auspicious alternative conventional methods detection contribute early diagnosis effective treatment. Graphical

Language: Английский

Citations

5