Highly Efficient Aggregation-Induced Electrochemiluminescence of Al(III)-Cbatpy Metal–Organic Gels Obtained by Ultrarapid Self-Assembly for a Biosensing Application DOI
Yue Zhang, Yifei Chen, Yamin Nie

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(35), P. 12196 - 12203

Published: Aug. 23, 2022

Aggregation-induced electrochemiluminescence (AIECL) has attracted extensive interest due to the significant increase in ECL response by restricting free intramolecular rotation and torsion, but traditional AIECL emitters suffer from limited efficiency, high cost, complex synthetic steps, dramatically limiting their applications. Herein, novel Al(III)-Cbatpy metal–organic gels (Al(III)-Cbatpy-MOGs) with nanofiber morphology ultrarapid coordination of Al3+ 4′-carboxylic acid-2,2′:6′,2″-terpyridine (Cbatpy) are developed, which demonstrates an excellent enhancement behavior far beyond that reported supramolecular gels. In view strong affinity N O atoms Cbatpy toward Al3+, Al(III)-Cbatpy-MOGs viscosity stability can be assembled one step within about 15 s, easily conquering main predicaments current emitters: complicated synthesis steps poor film formation. Impressively, efficiency superemission is 20 times higher than individual molecules, attributed aggregation organic ligand torsion reduce nonradiative relaxation. Furthermore, compared metal complexes, show benefits remarkable biocompatibility low cost without involvement any solvents, noble metals, rare metals. As proof, a "signal-off" sensing platform based on Al(III)-Cbatpy-MOGs/S2O82– system was constructed for sensitive detection dopamine (DA) limit 0.34 nM. This strategy provides method prepare cheap as highly efficient emitter, promising luminescent molecular device biosensor clinical diagnostic

Language: Английский

Single Biomolecule Imaging by Electrochemiluminescence DOI
Yujie Liu, Hongding Zhang,

Binxiao Li

et al.

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(43), P. 17910 - 17914

Published: Oct. 22, 2021

Herein, a single biomolecule is imaged by electrochemiluminescence (ECL) using Ru(bpy)32+-doped silica/Au nanoparticles (RuDSNs/AuNPs) as the ECL nanoemitters. The emission confined to local surface of RuDSNs leading significant enhancement in intensity. To prove concept, protein molecule at electrode initially visualized as-prepared RuDSN/AuNPs Furthermore, nanoemitter-labeled antibody linked cellular membrane image one cell, without interference current and optical background. success single-biomolecule imaging solves long-lasting task ultrasensitive analysis, which should be able provide more elegant information about biology.

Language: Английский

Citations

220
Single Cell Imaging of Electrochemiluminescence‐Driven Photodynamic Therapy DOI
Mingming Chen, Conghui Xu, Wei Zhao

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(16)

Published: Feb. 15, 2022

Abstract We report a photodynamic therapy driven by electrochemiluminescence (ECL). The luminescence generated Ru(bpy) 3 2+ and co‐reactant tripropylamine (TPA) pair acts as both optical readout for ECL imaging, light source the excitation of photosensitizer to produce reactive oxygen species (ROS) in (PDT) system. ECL‐driven PDT (ECL‐PDT) relies on effective energy transfer from emission chlorin e6 (Ce6), which sensitizes surrounding O 2 into ROS. dynamic process gradual morphological changes, variation cell‐matrix adhesions, well increase cell membrane permeability ECL‐PDT were monitored under microscopy (ECLM) with good spatiotemporal resolution. Combining real‐time imaging ECL‐PDT, this new strategy provides not only insights cellular processes, but also promising potential clinical applications.

Language: Английский

Citations

78
Nanoconfinement-Enhanced Electrochemiluminescence for in Situ Imaging of Single Biomolecules DOI

Yanwei Lu,

Xuedong Huang,

Shurong Wang

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(4), P. 3809 - 3817

Published: Feb. 17, 2023

Direct imaging of electrochemical reactions at the single-molecule level is potential interest in materials, diagnostic, and catalysis applications. Electrochemiluminescence (ECL) offers opportunity to convert redox events into photons. However, it challenging capture single photons emitted from a ECL reaction specific location, thus limiting high-quality We developed nanoreactors based on Ru(bpy)32+-doped nanoporous zeolite nanoparticles (Ru@zeolite) for direct visualization nanoconfinement-enhanced reactions. Each nanoreactor not only acts as matrix host Ru(bpy)32+ molecules but also provides nanoconfined environment collision co-reactant radicals realize efficient situ The nanoscale confinement resulted enhanced ECL. Using such probes, dual-signal sensing protocol visual tracking biomolecule was performed. High-resolution membrane proteins heterogeneous cells effectively addressed with near-zero backgrounds. This could provide more sensitive tool individual biomolecules significantly advance biological

Language: Английский

Citations

68
Gold Microbeads Enabled Proximity Electrochemiluminescence for Highly Sensitive and Size-Encoded Multiplex Immunoassays DOI

Xinrui Yang,

Junmeng Hang,

Weiyu Qu

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(29), P. 16026 - 16036

Published: July 17, 2023

Developing highly sensitive multiplex immunoassays is urgently needed to guide medical research and improve clinical diagnosis. Here, we report the proximity electrochemiluminescence (ECL) generation enabled by gold microbeads (GMBs) for improving detection sensitivity multiplexing capacity of ECL (ECLIAs). As demonstrated microscopy finite element simulation, GMBs can function as spherical ultramicroelectrodes triggering reactions in solutions. Employing solid carriers bead-based ECLIA, electrochemical oxidation a coreactant occur at both GMB surface substrate electrode, allowing radicals diffuse only short distance ∼100 nm react with luminophores that are labeled on surface. The via this low potential (LOP) route results 21.7-fold increase turnover frequency compared non-conductive rely exclusively conventional LOP route. Moreover, not restricted diffusion short-lived radicals, which enables simultaneous determination multiple acute myocardial infarction biomarkers using size-encoded GMB-based ECLIAs. This work brings new insight into understanding mechanisms may advance practical use

Language: Английский

Citations

64
Imaging of Single Bacteria with Electrochemiluminescence Microscopy DOI
Yuan Zhou, Jinrun Dong,

Pinlong Zhao

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(16), P. 8947 - 8953

Published: April 11, 2023

Rapid and accurate identification of pathogens is crucial for public healthcare patient treatment. However, the commonly used analytic tools such as molecular diagnostics mass spectrometry are either expensive or have long turnaround times sample purification amplification. Here, we introduce electrochemiluminescence (ECL) microscopy with a high spatiotemporal resolution unique chemical contrast to image identify single bacteria. Direct bacterial counting classification an accuracy up 90.5% demonstrated. We further report novel tunable ECL imaging mode which can switch from negative without labeling positive adsorption tris(2,2'-bipyridyl) ruthenium(II) imaging. With this tuning effect, single-molecule employed microscopic structures This work shows that offer powerful quantitative methodology information characterization.

Language: Английский

Citations

52
Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence DOI Creative Commons
Julie Descamps,

Camille Colin,

Gilles Tessier

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(16)

Published: Feb. 22, 2023

Here we report on a label-free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3 ]2+ luminophore. This work addresses central point minimal concentration ECL luminophore required to image single entities. We demonstrate possibility record images cells and mitochondria at down nM pM. is 7 orders magnitude lower than classically-used corresponds few hundreds luminophores diffusing around biological Yet, it produces remarkably sharp negative optical contrast images, as demonstrated by structural similarity index metric analyses supported predictions covering time. Finally, show that reported approach simple, fast, highly sensitive method, which opens new avenues for ultrasensitive imaging reactivity molecule level.

Language: Английский

Citations

47
Electrochemiluminescence Microscopy DOI Creative Commons
Sara Knežević, Dongni Han, Baohong Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(29)

Published: May 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Language: Английский

Citations

24
A Guide Inside Electrochemiluminescent Microscopy Mechanisms for Analytical Performance Improvement DOI Creative Commons
Sara Rebeccani, Alessandra Zanut, Claudio Santo

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 94(1), P. 336 - 348

Published: Dec. 15, 2021

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTA Guide Inside Electrochemiluminescent Microscopy Mechanisms for Analytical Performance ImprovementSara RebeccaniSara RebeccaniDepartment of Chemistry "Giacomo Ciamician", University Bologna, Bologna 40127, ItalyMore by Sara RebeccaniView Biography, Alessandra ZanutAlessandra ZanutTandon School Engineering, New York University, Brooklyn, 11201, United StatesMore ZanutView Claudio Ignazio SantoClaudio SantoDepartment SantoView Biographyhttps://orcid.org/0000-0003-4802-1671, Giovanni ValentiGiovanni ValentiDepartment ValentiView Biographyhttps://orcid.org/0000-0002-6223-2072, and Francesco Paolucci*Francesco PaolucciDepartment Italy*Email: [email protected]More PaolucciView Biographyhttps://orcid.org/0000-0003-4614-8740Cite this: Anal. Chem. 2022, 94, 1, 336–348Publication Date (Web):December 15, 2021Publication History Published online15 December 2021Published inissue 11 January 2022https://pubs.acs.org/doi/10.1021/acs.analchem.1c05065https://doi.org/10.1021/acs.analchem.1c05065review-articleACS PublicationsCopyright © 2021 The Authors. American Chemical Society. This publication is licensed under CC-BY 4.0. License Summary*You are free to share (copy redistribute) this article in any medium or format adapt (remix, transform, build upon) the material purpose, even commercially within parameters below:Creative Commons (CC): a Creative license.Attribution (BY): Credit must be given creator.View full license*DisclaimerThis summary highlights only some key features terms actual license. It not license has no legal value. Carefully review before using these materials. Open Access indicated. Learn MoreArticle Views5086Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views COUNTER-compliant sum text downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (6 MB) Get e-AlertscloseSUBJECTS:Electrochemical cells,Electrodes,Layers,Microscopy,Oxidation e-Alerts

Language: Английский

Citations

101
Shadow Electrochemiluminescence Microscopy of Single Mitochondria DOI

Yumeng Ma,

Camille Colin,

Julie Descamps

et al.

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(34), P. 18742 - 18749

Published: June 11, 2021

Mitochondria are the subcellular bioenergetic organelles. The analysis of their morphology and topology is essential to provide useful information on activity metabolism. Herein, we report a label-free shadow electrochemiluminescence (ECL) microscopy based spatial confinement ECL-emitting reactive layer image single living mitochondria deposited electrode surface. ECL mechanism freely-diffusing [Ru(bpy)3 ]2+ dye with sacrificial tri-n-propylamine coreactant restrains light-emitting region micrometric thickness allowing visualize individual remarkable sharp negative optical contrast. imaging approach named "shadow ECL" (SECL) reflects imprint local diffusional hindrance reagents by each mitochondrion. statistical colocalization spots functional revealed classical fluorescent biomarkers, MitoTracker Deep Red endogenous intramitochondrial NADH, validates reported methodology. versatility extreme sensitivity further demonstrated visualizing mitochondria, which remain hardly detectable usual biomarkers. Finally, alleviating problems photobleaching phototoxicity associated conventional methods, SECL should find promising applications in structures.

Language: Английский

Citations

82
Recent advances in electrochemiluminescence imaging analysis DOI Creative Commons

Chengda Meng,

Sara Knežević,

Fangxin Du

et al.

eScience, Journal Year: 2022, Volume and Issue: 2(6), P. 591 - 605

Published: Nov. 1, 2022

Electrochemiluminescence (ECL) has been widely applied in imaging owing to features that distinguish it from other microscopic techniques and electrochemical methods, including its high signal-to-noise ratio, remarkable sensitivity, wide linear range, spatiotemporal resolution, near-zero background light. Imaging technology based on ECL used the fields of immunosensing, pathological cell detection, drug analysis. Additionally, simple operation ability detect dynamic processes catalytic sites strengthen potential for research material surfaces interfaces, vivo biological analysis, visualization. At same time, emergence a variety nanomaterials new analysis equipment further promoted development high-resolution technology. This paper introduces mechanisms main systems. It then describes various forms reviews progress single-particle imaging, fingerprint structure single-cell imaging. Finally, authors offer their views about prospects

Language: Английский

Citations

66