A Close Look at Mechanism, Application, and Opportunities of Electrochemiluminescence Microscopy DOI Creative Commons

Xiaodan Gou,

Zejing Xing,

Cheng Ma

et al.

Chemical & Biomedical Imaging, Journal Year: 2023, Volume and Issue: 1(5), P. 414 - 433

Published: March 6, 2023

Electrochemiluminescence (ECL) is a typical luminescence process triggered by electrochemical reactions. Due to the separated signal types, ECL measurements have some merits of high sensitivity, low background, and simple configuration. Coupled with microscopy setup, (ECLM) has unique characteristics also furnished spatiotemporal resolution. Thus, many applications been created, including nanoscale sensing, mechanism deciphering, transient events single objects, ECLM crossover methods. In this review, we will overview development basic knowledge then profile setup design ECLM. Through understanding these two parts, next probe diverse ECLM, combining inter-relation each other. Finally, outlook discussing expectations further progress technology.

Language: Английский

MOF-818 nanozyme-based colorimetric and electrochemical dual-mode smartphone sensing platform for in situ detection of H2O2 and H2S released from living cells DOI
Kun Yu, Mengjie Li, Huining Chai

et al.

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 451, P. 138321 - 138321

Published: Aug. 1, 2022

Language: Английский

Citations

154
The New Era of High-Throughput Nanoelectrochemistry DOI Creative Commons
Xiangdong Xu, Dimitrios Valavanis,

Paolo Ciocci

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(1), P. 319 - 356

Published: Jan. 10, 2023

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTThe New Era of High-Throughput NanoelectrochemistryXiangdong XuXiangdong XuDepartment Chemistry, University Warwick, Coventry CV4 7AL, U.K.More by Xiangdong XuView Biographyhttps://orcid.org/0000-0002-1120-4661, Dimitrios ValavanisDimitrios ValavanisDepartment ValavanisView Biographyhttps://orcid.org/0000-0002-8777-664X, Paolo CiocciPaolo CiocciUniversité Paris Cité, ITODYS, CNRS, F-75013 Paris, FranceMore CiocciView Biography, Samuel ConfederatSamuel ConfederatSchool Electronic and Electrical Engineering Pollard Institute, Leeds, Leeds LS2 9JT, U.K.Bragg Centre for Materials Research, ConfederatView Fabio MarcuccioFabio MarcuccioSchool U.K.Faculty Medicine, Imperial College London, London SW7 2AZ, United KingdomMore MarcuccioView Biographyhttps://orcid.org/0000-0003-4816-2896, Jean-François LemineurJean-François LemineurUniversité LemineurView Actis*Paolo ActisSchool U.K.*[email protected]More ActisView Biographyhttps://orcid.org/0000-0002-7146-1854, Frédéric Kanoufi*Frédéric KanoufiUniversité France*[email KanoufiView Biographyhttps://orcid.org/0000-0002-9784-2380, Patrick R. Unwin*Patrick UnwinDepartment UnwinView Biographyhttps://orcid.org/0000-0003-3106-2178Cite this: Anal. Chem. 2023, 95, 1, 319–356Publication Date (Web):January 10, 2023Publication History Received15 November 2022Published online10 January 2023Published inissue 10 2023https://doi.org/10.1021/acs.analchem.2c05105Copyright © 2023 The Authors. Published American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceCC: Creative CommonsBY: Credit must be given to the creatorArticle Views1582Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (5 MB) Get e-AlertsSUBJECTS:Ions,Nanoparticles,Nanopores,Probes,Surface charge e-Alerts

Language: Английский

Citations

91
Electrochemiluminescence biosensors for detection of cancer biomarkers in biofluids: Principles, opportunities, and challenges DOI
Ahmed Barhoum, Zeynep Altıntaş,

K. S. Shalini Devi

et al.

Nano Today, Journal Year: 2023, Volume and Issue: 50, P. 101874 - 101874

Published: May 18, 2023

Language: Английский

Citations

86
Operando Imaging of Chemical Activity on Gold Plates with Single‐Molecule Electrochemiluminescence Microscopy DOI
Jinrun Dong, Yang Xu, Ziqing Zhang

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(14)

Published: Jan. 27, 2022

Abstract Classical electrochemical characterization tools cannot avoid averaging between the active reaction sites and their support, thus obscuring intrinsic roles. Single‐molecule techniques are in high demand. Here, we demonstrate super‐resolution imaging of Ru(bpy) 3 2+ based reactions on Au plates using single‐molecule electrochemiluminescence microscopy. By converting signals into optical signals, manage to achieve ultimate sensitivity single‐entity chemistry, that is directly resolving single photons from individual reactions. High spatial resolution, up 37 nm, further enables mapping chemical activity kinetics. The spatiotemporally resolved dynamic structure–activity relationship shows restructuring catalysts plays an important role determining reactivity. Our approach may lead gaining new insights towards evaluating designing electrocatalytic systems.

Language: Английский

Citations

71
Nanoconfinement-Enhanced Electrochemiluminescence for in Situ Imaging of Single Biomolecules DOI

Yanwei Lu,

Xuedong Huang,

Shurong Wang

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(4), P. 3809 - 3817

Published: Feb. 17, 2023

Direct imaging of electrochemical reactions at the single-molecule level is potential interest in materials, diagnostic, and catalysis applications. Electrochemiluminescence (ECL) offers opportunity to convert redox events into photons. However, it challenging capture single photons emitted from a ECL reaction specific location, thus limiting high-quality We developed nanoreactors based on Ru(bpy)32+-doped nanoporous zeolite nanoparticles (Ru@zeolite) for direct visualization nanoconfinement-enhanced reactions. Each nanoreactor not only acts as matrix host Ru(bpy)32+ molecules but also provides nanoconfined environment collision co-reactant radicals realize efficient situ The nanoscale confinement resulted enhanced ECL. Using such probes, dual-signal sensing protocol visual tracking biomolecule was performed. High-resolution membrane proteins heterogeneous cells effectively addressed with near-zero backgrounds. This could provide more sensitive tool individual biomolecules significantly advance biological

Language: Английский

Citations

68
Gold Microbeads Enabled Proximity Electrochemiluminescence for Highly Sensitive and Size-Encoded Multiplex Immunoassays DOI

Xinrui Yang,

Junmeng Hang,

Weiyu Qu

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(29), P. 16026 - 16036

Published: July 17, 2023

Developing highly sensitive multiplex immunoassays is urgently needed to guide medical research and improve clinical diagnosis. Here, we report the proximity electrochemiluminescence (ECL) generation enabled by gold microbeads (GMBs) for improving detection sensitivity multiplexing capacity of ECL (ECLIAs). As demonstrated microscopy finite element simulation, GMBs can function as spherical ultramicroelectrodes triggering reactions in solutions. Employing solid carriers bead-based ECLIA, electrochemical oxidation a coreactant occur at both GMB surface substrate electrode, allowing radicals diffuse only short distance ∼100 nm react with luminophores that are labeled on surface. The via this low potential (LOP) route results 21.7-fold increase turnover frequency compared non-conductive rely exclusively conventional LOP route. Moreover, not restricted diffusion short-lived radicals, which enables simultaneous determination multiple acute myocardial infarction biomarkers using size-encoded GMB-based ECLIAs. This work brings new insight into understanding mechanisms may advance practical use

Language: Английский

Citations

64
Recent advances and challenges in developing electrochemiluminescence biosensors for health analysis DOI

Yuxi Wei,

Honglan Qi, Chengxiao Zhang

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(24), P. 3507 - 3522

Published: Jan. 1, 2023

Recent advances and challenges in developing electrochemiluminescence biosensors for health analysis are reviewed.

Language: Английский

Citations

49
Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence DOI Creative Commons
Julie Descamps,

Camille Colin,

Gilles Tessier

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(16)

Published: Feb. 22, 2023

Here we report on a label-free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3 ]2+ luminophore. This work addresses central point minimal concentration ECL luminophore required to image single entities. We demonstrate possibility record images cells and mitochondria at down nM pM. is 7 orders magnitude lower than classically-used corresponds few hundreds luminophores diffusing around biological Yet, it produces remarkably sharp negative optical contrast images, as demonstrated by structural similarity index metric analyses supported predictions covering time. Finally, show that reported approach simple, fast, highly sensitive method, which opens new avenues for ultrasensitive imaging reactivity molecule level.

Language: Английский

Citations

47
Timescale correlation of shallow trap states increases electrochemiluminescence efficiency in carbon nitrides DOI Creative Commons
Yanfeng Fang, Hong Yang,

Yuhua Hou

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 27, 2024

Abstract Highly efficient interconversion of different types energy plays a crucial role in both science and technology. Among them, electrochemiluminescence, an emission light excited by electrochemical reactions, has drawn attention as powerful tool for bioassays. Nonetheless, the large differences timescale among diverse charge-transfer pathways from picoseconds to seconds significantly limit electrochemiluminescence efficiency hamper their broad applications. Here, we report coordination strategy improve carbon nitrides engineering shallow electron trap states via Au-N bond functionalization. Quantitative kinetics measurements theoretic calculations jointly disclose that bonds endow states, which coordinate fast transfer bulk emitter slow redox reaction co-reagent at diffusion layers. The ultimately accelerate rate emissive electron-hole recombination, setting new cathodic record nitrides, empowering visual sensor nitrite ion, typical environmental contaminant, with superior detection range limit.

Language: Английский

Citations

32
Electrochemiluminescence Microscopy DOI Creative Commons
Sara Knežević, Dongni Han, Baohong Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(29)

Published: May 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Language: Английский

Citations

24