Activatable Probes for Ratiometric Imaging of Endogenous Biomarkers In Vivo

ACS Nano, Journal Year: 2024, Volume and Issue: 18(5), P. 3916 - 3968

Published: Jan. 23, 2024

Dynamic variations in the concentration and abnormal distribution of endogenous biomarkers are strongly associated with multiple physiological pathological states. Therefore, it is crucial to design imaging systems capable real-time detection dynamic changes for accurate diagnosis effective treatment diseases. Recently, ratiometric has emerged as a widely used technique sensing due its advantage circumventing limitations inherent conventional intensity-dependent signal readout methods while also providing built-in self-calibration correction. Here, recent progress probes their applications outlined. Ratiometric classified according mechanisms, photoacoustic imaging, optical including photoluminescence self-luminescence magnetic resonance dual-modal discussed. The such pH, reactive oxygen species (ROS), nitrogen (RNS), glutathione (GSH), gas molecules, enzymes, metal ions, hypoxia discussed detail. Additionally, this Review presents an overview challenges faced field along future research directions.

Language: Английский

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Exogenously and Endogenously Sequential Regulation of DNA Nanodevices Enables Organelle‐Specific Signal Amplification in Subcellular ATP Profiling

Angewandte Chemie International Edition, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Adenosine triphosphate (ATP), the primary energy currency in cells, is dynamically regulated across different subcellular compartments. The ATP interplay between mitochondria and endoplasmic reticulum (ER) underscores their coordinated roles various biochemical processes, highlighting necessity for precise profiling of dynamics. Here we present an exogenously endogenously dual‐regulated DNA nanodevice spatiotemporally selective, subcellular‐compartment specific signal amplification sensing. system allows exogenous NIR light‐controlled spatiotemporal localization activation aptamer sensor or ER, while a endogenous enzyme organelles further drives via consumption molecular beacon fuels, resulting significantly enhanced sensitivity spatial precision organelle interest. Furthermore, demonstrate application this robust monitoring fluctuations ER following drug interventions. This advancement provides powerful tool improving our understanding cellular energetics at level holds potential development targeted therapeutics.

Language: Английский

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Theranostic nanoparticles with disease-specific administration strategies

Nano Today, Journal Year: 2021, Volume and Issue: 42, P. 101335 - 101335

Published: Nov. 10, 2021

Language: Английский

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Construction of Fast-Walking Tetrahedral DNA Walker with Four Arms for Sensitive Detection and Intracellular Imaging of Apurinic/Apyrimidinic Endonuclease 1

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(24), P. 8732 - 8739

Published: June 9, 2022

Herein, a novel tetrahedral DNA walker with four arms was engineered to travel efficiently on the 3D-tracks via catalyzed hairpin assembly autonomously, realizing sensitive detection and activity assessment as well intracellular imaging of apurinic/apyrimidinic endonuclease 1 (APE1). In contrast traditional walkers, rigid 3D framework structure nonplanar multi-sites walking endowed high collision efficiency, showing fast rate nuclease resistance. Impressively, initial 4.54 times faster than that free bipedal produced significant fluorescence recovery in about 40 min, achieving APE1 low limit 5.54× 10–6 U/μL ultrasensitive activation imaging. This strategy provides for accurate identification low-abundance cancer biomarker potential medical diagnosis.

Language: Английский

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Unraveling and Overcoming Platinum Drug‐Resistant Cancer Tumors with DNA Nanostructures

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 33(2)

Published: Nov. 2, 2022

Abstract All chemotherapeutic treatments worldwide (>50%) use platinum‐based compounds. Despite their clinical success, an increasing number of platinum drug‐resistant tumors are reported, limiting the therapeutic application these While various kinds strategies pursued to circumvent resistances, there remains a lack understanding how cancer cells develop drug resistances. Within this study, involvement DNA repair enzyme apurinic/apyrimidinic endonuclease 1 (APE1) in occurrence resistance is directly visualized living by tetrahedron‐based molecular probe. Capitalizing on biochemical insight, suppression expression APE1 realized using RNA interference technique, presenting novel strategy overcome resistance. This study presents first example Pt(IV) complex loaded tumor aptamer‐modified tetrahedrons with specific small interfering (siRNA) strand for and treatment patient‐derived lung tumors.

Language: Английский

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Construction of an endogenously activated catalytic DNA circuit for highly robust in vivo microRNA imaging

Nano Today, Journal Year: 2022, Volume and Issue: 45, P. 101553 - 101553

Published: July 14, 2022

Language: Английский

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Target-mediated self-assembly of DNA networks for sensitive detection and intracellular imaging of APE1 in living cells

Chemical Science, Journal Year: 2023, Volume and Issue: 14(9), P. 2318 - 2324

Published: Jan. 1, 2023

Herein, giant DNA networks were assembled from two kinds of functionalized tetrahedral nanostructures (f-TDNs) for sensitive detection and intracellular imaging apurinic/apyrimidinic endonuclease 1 (APE1) as well gene therapy in tumor cells. Impressively, the reaction rate catalytic hairpin assembly (CHA) on f-TDNs was much faster than that conventional free CHA owing to high local concentration hairpins, spatial confinement effect production networks, which significantly enhanced fluorescence signal achieve APE1 with a limit 3.34 × 10-8 U μL-1. More importantly, aptamer Sgc8 could enhance targeting activity structure cells, allowing it endocytose into cells without any transfection reagents, selective living Meanwhile, siRNA carried by f-TDN1 be accurately released promote cell apoptosis presence endogenous target APE1, realizing effective precise therapy. Benefiting specificity sensitivity, developed provide an excellent nanoplatform cancer diagnosis

Language: Английский

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Molecular imaging: design mechanism and bioapplications

Science China Chemistry, Journal Year: 2023, Volume and Issue: 66(5), P. 1336 - 1383

Published: March 3, 2023

Language: Английский

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Optical and Photoacoustic Imaging In Vivo: Opportunities and Challenges

Chemical & Biomedical Imaging, Journal Year: 2023, Volume and Issue: 1(2), P. 99 - 109

Published: March 20, 2023

Optical and photoacoustic imaging plays an important role in biomedical applications owing to its noninvasiveness high resolution. Fluorescence emerge as powerful tools deconstruct molecular information investigate biological processes vivo. Despite great progress has been achieved chemical probe synthesis, how design probes with optimal fluorescence or performance dynamically visualize the process vivo still faces challenges. From this perspective, we will focus on advanced development of Furthermore, concerns prospects for future be demonstrated.

Language: Английский

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Enzyme-Triggered DNA Sensor Technology for Spatially-Controlled, Cell-Selective Molecular Imaging

Accounts of Chemical Research, Journal Year: 2023, Volume and Issue: 56(12), P. 1482 - 1493

Published: June 1, 2023

With unparalleled programmability, DNA has evolved as a powerful scaffold for engineering intricate and dynamic systems that can perform diverse tasks. By allowing serial detection of molecular targets in complex cellular milieus, increasingly sophisticated sensors have not only promoted significant advances unveiling the fundamental mechanisms various pathophysiological processes but also provided useful toolkit disease diagnostics based on signatures. Despite much progress, an inherent limitation DNA-based is they often lack spatial control cell-type selectivity sensing activity because their "always active" design mechanism. Since most interests are exclusive to cells, shared by normal application such biosensors disease-specific imaging limited inadequate signal-to-background ratios due indistinguishable signal response both cells. Therefore, imparting with controllability remains key issue achieve high sensitivity cell specificity.As biocatalyst, enzyme been found be closely related pathological conditions numerous diseases. For example, many nucleases, protease, kinases identified overexpressed cells considered important biomarkers cancer, inflammation, neurological Recently, we envisioned pathophysiology-associated enzymes could leveraged endogenous triggers over improved cell-specificity. In this Account, outline research efforts from our group development enzyme-triggered, sensor technology enables spatially controlled, selective imaging. programmable further enzymatically cleavable sites, series DNAzyme- aptamer-based developed enzyme-controlled (e.g., metal ions small molecules) cancer cell-selective manner. particular, introduction PNA bridge molecules engineer functional peptides, conceptual protease-activated established spatioselective extracellular tumor microenvironments. Furthermore, enzyme-triggered amplification approaches, activated beacon catalytic hairpin assembly, RNA specific inflammatory cells), which enhanced disease-site specificity thus ratio. The strategy expanded amplified non-RNA species through combination design. Finally, challenges potential future directions burgeoning field discussed. We hope Account offers insights into rational platforms opening new frontiers resolved, believe continuing together discoveries disease-associated will promise usher era diagnosis.

Language: Английский

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