Smartphone-assisted self-powered dual-mode biosensor designed on binary 3D DNA Walkers mediated CRISPR/Cas12a system

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 483, P. 149231 - 149231

Published: Jan. 30, 2024

Language: Английский

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Target-mediated self-assembly of DNA networks for sensitive detection and intracellular imaging of APE1 in living cells

Chemical Science, Journal Year: 2023, Volume and Issue: 14(9), P. 2318 - 2324

Published: Jan. 1, 2023

Herein, giant DNA networks were assembled from two kinds of functionalized tetrahedral nanostructures (f-TDNs) for sensitive detection and intracellular imaging apurinic/apyrimidinic endonuclease 1 (APE1) as well gene therapy in tumor cells. Impressively, the reaction rate catalytic hairpin assembly (CHA) on f-TDNs was much faster than that conventional free CHA owing to high local concentration hairpins, spatial confinement effect production networks, which significantly enhanced fluorescence signal achieve APE1 with a limit 3.34 × 10-8 U μL-1. More importantly, aptamer Sgc8 could enhance targeting activity structure cells, allowing it endocytose into cells without any transfection reagents, selective living Meanwhile, siRNA carried by f-TDN1 be accurately released promote cell apoptosis presence endogenous target APE1, realizing effective precise therapy. Benefiting specificity sensitivity, developed provide an excellent nanoplatform cancer diagnosis

Language: Английский

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Closed Cyclic DNA Machine for Sensitive Logic Operation and APE1 Detection

Small, Journal Year: 2023, Volume and Issue: 19(23)

Published: March 14, 2023

DNA self-assembly has been developed as a kind of robust signal amplification strategy, but most reported assembly pathways are programmed to amplify in one direction. Herein, based on mutual-activated cascade cycle hybridization chain reaction (HCR) and catalytic hairpin (CHA), closed circuit (CCC) machine is for sensitive logic operation molecular recognition. Benefiting from the synergistically accelerated amplification, cyclic enabled computing with strong significant output signals even at weak input signals. The typical operations such OR, YES, AND, INHIBIT, NOR, NAND gate, conveniently clearly executed this through rational design elements. Moreover, by integrating target recognition module CCC module, proposed further employed homogeneous detection apurinic/apyrimidinic endonuclease 1 (APE1). precise exponential facilitated highly selective APE1 limit (LOD) 7.8 × 10-5 U mL-1 . Besides, normal cells tumor distinguished unambiguously method according detected concentration difference cellular APE1, which indicates robustness practicability method.

Language: Английский

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Well-Aligned Track-Accelerated Tripedal DNA Walker for Photoelectrochemical Recognition of Dual-miRNAs Based on Molecular Logic Gates

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(13), P. 5764 - 5772

Published: March 24, 2023

Post-transcriptional regulators, microRNAs (miRNAs), are involved in the occurrence and progression of various diseases. However, due to complexity disease-related miRNA regulatory networks, typing identification miRNAs have remained challenging. Herein, a linear ladder-like DNA nanoarchitecture (LDN) was constructed promote movement efficiency tripedal walker (T-walker), which combined with DNA-based logic gates PTCDA@PDA/CdS/WO3 photoelectrode develop novel biosensor for detection dual-miRNAs. Two miRNAs, miR-122 miR-21, were used as targets operate module, while its output, trigger strands (TSs), initiated catalytic hairpin assembly (CHA) reaction form T-walker. By using LDN track, T-walker efficiently unfolded 4, further hybridized alkaline phosphatase-modified 5 (AP-H5). The remaining AP can catalyze ascorbic acid 2-phosphate (AAP) into (AA), an ideal electron donor, thus resulting photocurrent change. signals both AND OR displayed relationship logarithm dual-miRNA concentrations limits 10.1 13.6 fM, respectively. Moreover, intelligent rational design tracks gives impetus create well-organized sensing interface wide application clinical diagnosis cancer monitoring.

Language: Английский

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Development of a DNAzyme Walker for the Detection of APE1 in Living Cancer Cells

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(40), P. 14990 - 14997

Published: Sept. 19, 2023

DNAzyme walker technology is a compelling option for bioanalytical and drug delivery applications. While nucleic acid protein targets have been used to activate walkers, investigations into enzyme-triggered walkers in living cells are still their early stages. The base excision repair (BER) pathway presents an array of enzymes that overexpressed cancer cells. Here, we introduce system sensitively specifically detects the BER enzyme apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1). We constructed on surface 20 nm-diameter gold nanoparticles. achieved detection limit 160 fM APE1 buffer whole cell lysate equivalent amount single HeLa sample volume 100 μL. Confocal imaging walking reveals cytoplasmic distribution Walking activity tunable exogenous Mn2+ concentrations uptake does not require transfection assistance. demonstrate investigative potential up-regulated or overactive biomarkers

Language: Английский

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Nanoconfined Silver Nanoclusters Combined with X-Shaped DNA Recognizer-Triggered Cascade Amplification for Electrochemiluminescence Detection of APE1

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11383 - 11389

Published: July 1, 2024

Apurinic/apyrimidinic endonuclease 1 (APE1), as a vital base excision repair enzyme, is essential for maintaining genomic integrity and stability, its abnormal expression closely associated with malignant tumors. Herein, we constructed an electrochemiluminescence (ECL) biosensor detecting APE1 activity by combining nanoconfined ECL silver nanoclusters (Ag NCs) X-shaped DNA recognizer-triggered cascade amplification. Specifically, the Ag NCs were prepared confined in glutaraldehyde-cross-linked chitosan hydrogel network using one-pot method, resulting strong response exceptional stability comparison discrete NCs. Furthermore, self-assembled recognizers designed detection, which not only improved reaction kinetics due to ordered arrangement of recognition sites but also achieved high sensitivity utilizing amplification strand displacement (SDA) DNAzyme catalysis. As expected, this sensitive detection range 1.0 × 10–3 U·μL–1 10–10 limit 2.21 10–11 U·μL–1, rendering it desirable approach biomarker detection.

Language: Английский

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Ultrahigh-Speed 3D DNA Walker with Dual Self-Protected DNAzymes for Ultrasensitive Fluorescence Detection and Intracellular Imaging of microRNA

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(24), P. 9866 - 9875

Published: June 5, 2024

Herein, a dual self-protected DNAzyme-based 3D DNA walker (dSPD walker), composed of activated walking particles (ac-dSPWPs) and track (TPs), was constructed for ultrasensitive ultrahigh-speed fluorescence detection imaging microRNAs (miRNAs) in living cells. Impressively, compared with the defect that "one" target miRNA only initiates arm conventional single DNAzyme walker, dSPD benefits from secondary amplification spatial confinement effect could guide to generate "n" targets, thereby initiating nearby strands immediately, realizing initial rate over one-magnitude-order faster than one. Moreover, process relative motion between ac-dSPWPs TPs, cleave multiple substrate simultaneously speed up movement reduce derailment rate, as well combine successive TPs facilitate large amount continuous signal accumulation, achieving an ultrafast miRNA-221 within 10 min vitro high sensitivity low limit 0.84 pM. In addition, nanospheres obtained by rolling circle reaction can capture Cy5 dispersed liquids, which achieves high-contrast miRNA-221, resulting further cancer The proposed strategy has made bold innovation rapid sensitive intracellular low-abundance biomarkers, offering promising application early diagnosis relevant research tumors.

Language: Английский

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Proximity-Induced Bipedal DNA Walker for Accurately Visualizing microRNA in Living Cancer Cell

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(26), P. 10669 - 10676

Published: June 24, 2024

DNA walker, a type of dynamic device that is capable moving progressively along prescribed walking tracks, has emerged as an ideal and powerful tool for biosensing bioimaging. However, most the reported three-dimensional (3D) walker were merely designed detection single target, they not achieving universal applicability. Herein, we first time development proximity-induced 3D bipedal imaging low abundance biomolecules. As proof concept, miRNA-34a, biomarker breast cancer, chosen model system to demonstrate this approach. In our design, can be generated only by specific recognition two proximity probes miRNA-34a. Meanwhile, it stochastically autonomously traveled on tracks (gold nanoparticles) via catalytic hairpin assembly (CHA), resulting in amplified fluorescence signal. comparison with some conventional walkers utilized living cell imaging, induced ligation assay greatly improve ensure high selectivity bioanalysis. By taking advantage these unique features, successfully realizes accurate effective monitoring target miRNA-34a expression levels cells, affording universal, valuable, promising platform low-abundance cancer identification cancer.

Language: Английский

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Cascade signal enhancement by integrating DNA walking and RCA reaction-assisted “silver-link” crossing electrode for ultrasensitive electrochemical detection of Staphylococcus aureus

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 217, P. 114716 - 114716

Published: Sept. 14, 2022

Language: Английский

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A Review of Recent Progress in Drug Doping and Gene Doping Control Analysis

Molecules, Journal Year: 2023, Volume and Issue: 28(14), P. 5483 - 5483

Published: July 18, 2023

The illicit utilization of performance-enhancing substances, commonly referred to as doping, not only infringes upon the principles fair competition within athletic pursuits but also poses significant health hazards athletes. Doping control analysis has emerged a conventional approach ensuring equity and integrity in sports. Over past few decades, extensive advancements have been made doping methods, catering escalating need for qualitative quantitative numerous banned substances exhibiting diverse chemical biological characteristics. Progress science, technology, instrumentation facilitated proliferation varied techniques detecting doping. In this comprehensive review, we present succinct overview recent research developments last ten years pertaining these detection methodologies. We undertake comparative analysis, evaluating merits limitations each technique, offer insights into prospective future methods. It is noteworthy that continual design synthesis novel synthetic agents compelled researchers constantly refine innovate methods order address ever-expanding range covertly employed agents. Overall, remain passive position are always on road control.

Language: Английский

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