Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Feb. 27, 2024
Abnormal
physiological
processes
and
diseases
can
lead
to
content
or
activity
fluctuations
of
biocomponents
in
organelles
whole
blood.
However,
precise
monitoring
these
abnormalities
remains
extremely
challenging
due
the
insufficient
sensitivity
accuracy
available
fluorescence
probes,
which
be
attributed
background
arising
from
two
sources,
1)
biocomponent
autofluorescence
(BCAF)
2)
probe
intrinsic
(PIF).
To
overcome
obstacles,
we
have
re-engineered
far-red
NIR
II
rhodol
derivatives
that
possess
weak
BCAF
interference.
And
a
series
"zero"
PIF
sensing-platforms
were
created
by
systematically
regulating
open-loop/spirocyclic
forms.
Leveraging
advancements,
devised
various
ultra-sensitive
indicators,
achieving
substantial
boosts
(190
1300-fold).
Among
8-LAP
demonstrated
accurate
tracking
quantifying
leucine
aminopeptidase
(LAP)
blood
at
stages
tumor
metastasis.
Furthermore,
coupling
with
an
endoplasmic
reticulum-targeting
element
enabled
detection
ERAP1
HCT116
cells
p53
abnormalities.
This
delicate
design
eliminating
provides
insights
into
enhancing
existing
probes
toward
imaging
abnormal
diseases.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(46)
Published: Sept. 28, 2023
Abstract
To
realize
sensing
and
labeling
biomarkers
is
quite
challenging
in
terms
of
designing
multimodal
imaging
probes.
In
this
study,
we
developed
a
novel
β
‐galactosidase
(
‐gal)
activated
bimodal
probe
that
combines
near‐infrared
(NIR)
fluorescence
magnetic
resonance
(MRI)
to
enable
real‐time
visualization
activity
living
organisms.
Upon
‐gal
activation,
Gal‐Cy‐Gd‐1
exhibits
remarkable
42‐fold
increase
NIR
intensity
at
717
nm,
allowing
covalent
adjacent
target
enzymes
or
proteins
avoiding
molecular
escape
promote
accumulation
the
tumor
site.
This
reaction
enhances
longitudinal
relaxivity
by
approximately
1.9
times,
facilitating
high‐resolution
MRI.
The
unique
features
precise
live
cells
mice.
probe's
utilization
aids
identifying
situ
ovarian
tumors,
offering
valuable
assistance
removal
tissue
during
surgical
procedures
fusion
MRI
activation
through
self‐immobilizing
provides
robust
approach
for
visualizing
activity.
Moreover,
sets
groundwork
developing
other
activatable
probes,
vivo
enzyme
localization.
Chemical Science,
Journal Year:
2023,
Volume and Issue:
14(11), P. 2928 - 2934
Published: Jan. 1, 2023
Small-molecule
probes
emitting
in
the
second
near-infrared
window
(NIR-II)
are
attracting
great
attention
because
of
their
deep-tissue
imaging
ability.
However,
developing
NIR-II
fluorogenic
(off-on)
with
good
water
solubility
remains
a
challenge
due
to
lack
facile
approach.
Herein
we
first
report
combination
changeable
π-conjugation
and
hydrophilic
groups
as
an
effective
strategy
for
water-soluble
probes.
With
strategy,
new
fluorophores
prepared,
among
which
NIR-II-F2
NIR-II-F3
show
superior
stability
bright
fluorescence
aqueous
media,
thus
used
design
two
leucine
aminopeptidase
(LAP).
The
excellent
performance
real
bio-environments
is
demonstrated
by
mouse
vasculatures
organs
NIR-II-F2,
LAP
drug-induced
liver
injury
mice
one
enzymatic
probes;
however,
water-insoluble
dyes
cannot
achieve
such
vivo
under
same
conditions.
Our
may
be
helpful
further
organic
other
analytes.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(48)
Published: Oct. 6, 2024
Rheumatoid
arthritis
(RA)
represents
an
insidious
autoimmune
inflammatory
disorder
that
severely
lowers
the
life
quality
by
progressively
destructing
joint
functions
and
eventually
causing
permanent
disability,
posing
a
serious
public
health
problem.
Here,
advanced
theranostic
probe
is
introduced
integrates
activatable
second
near-infrared
(NIR-II)
fluorescence
imaging
for
precise
RA
diagnosis
with
multi-pronged
treatments.
A
novel
molecular
comprising
long-wavelength
aggregation-induced
emission
unit
manganese
carbonyl
cage
motif
synthesized,
which
enables
NIR-II
activation
concurrently
releasing
therapeutic
carbon
monoxide
(CO)
gas
in
inflamed
microenvironment.
This
self-assembles
into
biocompatible
nanoprobe,
subsequently
conjugated
anti-IL-6R
antibody
to
afford
active-targeting
ability
of
RA.
The
nanoprobe
exhibits
significant
turn-on
signal
at
lesion,
enabling
highly
sensitive
real-time
monitoring.
combination
ROS
scavenging,
on-demand
CO
release,
IL-6
signaling
blockade
results
potent
effect
synergistic
immunomodulation
impact,
significantly
alleviating
symptoms
preventing
destruction.
research
introduces
paradigm
development
high-performance,
strategies
facilitate
detection
enhanced
treatment
RA-related
diseases.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Feb. 27, 2024
Abnormal
physiological
processes
and
diseases
can
lead
to
content
or
activity
fluctuations
of
biocomponents
in
organelles
whole
blood.
However,
precise
monitoring
these
abnormalities
remains
extremely
challenging
due
the
insufficient
sensitivity
accuracy
available
fluorescence
probes,
which
be
attributed
background
arising
from
two
sources,
1)
biocomponent
autofluorescence
(BCAF)
2)
probe
intrinsic
(PIF).
To
overcome
obstacles,
we
have
re-engineered
far-red
NIR
II
rhodol
derivatives
that
possess
weak
BCAF
interference.
And
a
series
"zero"
PIF
sensing-platforms
were
created
by
systematically
regulating
open-loop/spirocyclic
forms.
Leveraging
advancements,
devised
various
ultra-sensitive
indicators,
achieving
substantial
boosts
(190
1300-fold).
Among
8-LAP
demonstrated
accurate
tracking
quantifying
leucine
aminopeptidase
(LAP)
blood
at
stages
tumor
metastasis.
Furthermore,
coupling
with
an
endoplasmic
reticulum-targeting
element
enabled
detection
ERAP1
HCT116
cells
p53
abnormalities.
This
delicate
design
eliminating
provides
insights
into
enhancing
existing
probes
toward
imaging
abnormal
diseases.
